Title:
CELL SORTING DEVICE AND SORTING METHOD
Document Type and Number:
WIPO Patent Application WO/2020/000525
Kind Code:
A1
Abstract:
Disclosed is a cell sorting device, comprising a fluid pipe (1), a cell sorting unit (2), a cell delivery unit (3) and a magnetic field assembly (4). The cell sorting unit (2) comprises at least one cell sorting chamber (21), the fluid pipe (1) enables the cell sorting chamber (21) to be in communication in sequence, and magnetic beads modified with an antibody are contained in the cell sorting chamber (21). The types of cells bound to antibodies located in the same cell sorting chamber (21) are the same, and the type of cells bound to an antibody located in at least one cell sorting chamber (21) is different from the type of cells bound to antibodies located in the other cell sorting chambers (21). The specific identification and bonding of different types of cells are realized by means of the magnetic beads located in the cell sorting chamber (21), and the magnetic beads are attracted by means of a magnetic force generated by the magnetic field assembly (4), such that target cells are accumulated in a corresponding cell sorting chamber (21), thereby achieving the sorting of living cells, and having the advantages of high specificity and sensitivity and of needing no pretreatment on a sample solution. Further disclosed is a cell sorting method which uses the cell sorting device and by which the high-efficiency sorting of living cells can be achieved.
Inventors:
CHE TUANJIE (CN)
XU HONG (CN)
LI LIN (CN)
LI YAPENG (CN)
XU HONG (CN)
LI LIN (CN)
LI YAPENG (CN)
Application Number:
PCT/CN2018/095556
Publication Date:
January 02, 2020
Filing Date:
July 13, 2018
Export Citation:
Assignee:
SUZHOU BAIYUAN GENT CO LTD (CN)
International Classes:
C12M1/00; C12M1/42; C12N5/00; C12N13/00
Domestic Patent References:
| WO2018096005A1 | 2018-05-31 | |||
| WO2001036449A1 | 2001-05-25 |
Foreign References:
| US20070231888A1 | 2007-10-04 | |||
| CN102758259A | 2012-10-31 | |||
| CN103865752A | 2014-06-18 | |||
| CN102170971A | 2011-08-31 | |||
| US20160354773A1 | 2016-12-08 | |||
| CN102115711A | 2011-07-06 | |||
| CN204346918U | 2015-05-20 |
Attorney, Agent or Firm:
SUNSHINE INTELLECTUAL PROPERTY INTERNATIONAL CO., LTD (CN)
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