Title:
CELL SORTING
Document Type and Number:
WIPO Patent Application WO/2016/205745
Kind Code:
A3
Abstract:
The invention provides for use of CRISPR-Cas systems to sort barcoded cells or molecules. Cells or nucleic acid molecules may be sorted from a heterogenous population by targeting a barcode of interest specific for a cell or cell progeny or nucleic acid molecule of interest.
Inventors:
KONERMANN SILVANA (CH)
FELDMAN DAVID (US)
ZHANG FENG (US)
BLAINEY PAUL (US)
FELDMAN DAVID (US)
ZHANG FENG (US)
BLAINEY PAUL (US)
Application Number:
PCT/US2016/038234
Publication Date:
June 15, 2017
Filing Date:
June 17, 2016
Export Citation:
Assignee:
BROAD INST INC (UM)
MASSACHUSETTS INST TECHNOLOGY (US)
KONERMANN SILVANA (CH)
FELDMAN DAVID (US)
ZHANG FENG (US)
BLAINEY PAUL (US)
MASSACHUSETTS INST TECHNOLOGY (US)
KONERMANN SILVANA (CH)
FELDMAN DAVID (US)
ZHANG FENG (US)
BLAINEY PAUL (US)
International Classes:
C12N15/10
Domestic Patent References:
| WO2015006294A2 | 2015-01-15 |
Other References:
L. MALI ET AL. ET AL: "RNA guided human genome engineering via Cas9", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), US, pages 823 - 826, XP055300091, ISSN: 0036-8075, DOI: 10.1126/science.1232033
S. CONG ET AL: "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, vol. 339, no. 6121, 3 January 2013 (2013-01-03), US, pages 819 - 823, XP055300065, ISSN: 0036-8075, DOI: 10.1126/science.1229223
SILVANA KONERMANN ET AL: "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", NATURE, vol. 517, no. 7536, 10 December 2014 (2014-12-10), United Kingdom, pages 583 - 588, XP055294959, ISSN: 0028-0836, DOI: 10.1038/nature14136
PRASHANT MALI ET AL: "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, vol. 31, no. 9, 1 August 2013 (2013-08-01), pages 833 - 838, XP055186073, ISSN: 1087-0156, DOI: 10.1038/nbt.2675
BHANG HYO-EUN C ET AL: "Studying clonal dynamics in response to cancer therapy using high-complexity barcoding", NATURE MEDICINE, vol. 21, no. 5, May 2015 (2015-05-01), pages 440 - 448+4PP, XP002763724, ISSN: 1546-170X, DOI: 10.1038/nm.3841
SILVANA KONERMANN ET AL: "Optical control of mammalian endogenous transcription and epigenetic states", NATURE, 23 July 2013 (2013-07-23), XP055087168, ISSN: 0028-0836, DOI: 10.1038/nature12466
HECKL DIRK ET AL: "Toward Whole-Transcriptome Editing with CRISPR-Cas9", MOLECULAR CELL, vol. 58, no. 4, 21 May 2015 (2015-05-21), pages 560 - 562, XP029129116, ISSN: 1097-2765, DOI: 10.1016/J.MOLCEL.2015.05.016
SHAINA N PORTER ET AL: "Lentiviral and targeted cellular barcoding reveals ongoing clonal dynamics of cell lines in vitro and in vivo", GENOME BIOLOGY, vol. 15, no. 5, R75, 30 May 2014 (2014-05-30), BIOMED CENTRAL LTD., LONDON, GB, XP021191459, ISSN: 1465-6906, DOI: 10.1186/GB-2014-15-5-R75
PRASHANT MALI ET AL: "Barcoding cells using cell-surface programmable DNA-binding domains", NATURE METHODS, vol. 10, no. 5, 1 January 2013 (2013-01-01), GB, pages 403 - 406, XP055236686, ISSN: 1548-7091, DOI: 10.1038/nmeth.2407
S. CONG ET AL: "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, vol. 339, no. 6121, 3 January 2013 (2013-01-03), US, pages 819 - 823, XP055300065, ISSN: 0036-8075, DOI: 10.1126/science.1229223
SILVANA KONERMANN ET AL: "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", NATURE, vol. 517, no. 7536, 10 December 2014 (2014-12-10), United Kingdom, pages 583 - 588, XP055294959, ISSN: 0028-0836, DOI: 10.1038/nature14136
PRASHANT MALI ET AL: "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, vol. 31, no. 9, 1 August 2013 (2013-08-01), pages 833 - 838, XP055186073, ISSN: 1087-0156, DOI: 10.1038/nbt.2675
BHANG HYO-EUN C ET AL: "Studying clonal dynamics in response to cancer therapy using high-complexity barcoding", NATURE MEDICINE, vol. 21, no. 5, May 2015 (2015-05-01), pages 440 - 448+4PP, XP002763724, ISSN: 1546-170X, DOI: 10.1038/nm.3841
SILVANA KONERMANN ET AL: "Optical control of mammalian endogenous transcription and epigenetic states", NATURE, 23 July 2013 (2013-07-23), XP055087168, ISSN: 0028-0836, DOI: 10.1038/nature12466
HECKL DIRK ET AL: "Toward Whole-Transcriptome Editing with CRISPR-Cas9", MOLECULAR CELL, vol. 58, no. 4, 21 May 2015 (2015-05-21), pages 560 - 562, XP029129116, ISSN: 1097-2765, DOI: 10.1016/J.MOLCEL.2015.05.016
SHAINA N PORTER ET AL: "Lentiviral and targeted cellular barcoding reveals ongoing clonal dynamics of cell lines in vitro and in vivo", GENOME BIOLOGY, vol. 15, no. 5, R75, 30 May 2014 (2014-05-30), BIOMED CENTRAL LTD., LONDON, GB, XP021191459, ISSN: 1465-6906, DOI: 10.1186/GB-2014-15-5-R75
PRASHANT MALI ET AL: "Barcoding cells using cell-surface programmable DNA-binding domains", NATURE METHODS, vol. 10, no. 5, 1 January 2013 (2013-01-01), GB, pages 403 - 406, XP055236686, ISSN: 1548-7091, DOI: 10.1038/nmeth.2407
Attorney, Agent or Firm:
SCHER, Michael, B. et al. (US)
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