Title:
CELL-SPREADING METHOD AND CELL-SPREADING KIT FOR OBSERVING RARE CELLS
Document Type and Number:
WIPO Patent Application WO/2017/073533
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a means for increasing the efficiency of recovering rare cells in a cell suspension by suppressing adsorption of the cells to the experimental equipment without inhibiting adsorption of the cells to a cell-spreading substrate. A cell-spreading method according to the present invention includes: a step for adding protein to a cell suspension in such an amount that the final concentration is 0.0001-0.1 %, or, preferably, 0.0005-0.01 % (protein adding step); and a step for making cells in the cell suspension, which has been subjected to the protein adding step, spread on, stand still on, and adsorb to a cell-spreading substrate in which the contact angle with water is equal to or greater than 60º or, preferably, 70-90º respect to (cell spreading step).
Inventors:
ARAKI JUNGO (JP)
MASUBUCHI MANAMI (JP)
MASUBUCHI MANAMI (JP)
Application Number:
PCT/JP2016/081512
Publication Date:
May 04, 2017
Filing Date:
October 25, 2016
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
C12M1/26; C12N1/02; C07K14/765; C12N1/00; C12N5/07
Domestic Patent References:
| WO2014061675A1 | 2014-04-24 |
Foreign References:
| JP2006296427A | 2006-11-02 | |||
| JP2009000012A | 2009-01-08 |
Other References:
CHEN, MENG. ET AL.: "Cell attachment and biocompatibility of polytetrafluoroethylene (PTFE) treated with glow-discharge plasma of mixed ammonia and oxygen", JOURNAL OF BIOMATERIALS SCIENCE, vol. 14, no. 9, 2003, pages 917 - 935, XP055379527
IKEDA, Y.: "Surface modification of polymers for medical applications", BIOMATERIALS, vol. 15, no. 10, 1994, pages 725 - 736, XP024142207
See also references of EP 3369804A4
IKEDA, Y.: "Surface modification of polymers for medical applications", BIOMATERIALS, vol. 15, no. 10, 1994, pages 725 - 736, XP024142207
See also references of EP 3369804A4
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
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