Title:
CELL-STAINING METHOD AND SPECIMEN COLLECTION TUBE FOR USE IN METHOD
Document Type and Number:
WIPO Patent Application WO/2014/185151
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a simple cell-staining method which takes less time and is used in a step for staining cells in a liquid specimen, and prevents damage to or loss (spillage) of cells in the collected specimen, when specifically staining a specific substance expressed in cells to be detected in the liquid specimen. The present invention also addresses the problem of providing a specimen collection tube suitable for use with this cell-staining method. The present invention solves these problems by using a method for specifically staining a specific substance present in cells in a specimen, the method involving the execution of the following steps as one step: (A) a step for immobilizing the cells, and (B) a step for soaking the cells.
Inventors:
CHIYODA TSUNEKO (JP)
ARAKI JUNGO (JP)
ARAKI JUNGO (JP)
Application Number:
PCT/JP2014/058029
Publication Date:
November 20, 2014
Filing Date:
March 24, 2014
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/48; C12M1/26; C12M1/34; C12Q1/04; G01N1/30; G01N33/53; G01N33/533
Domestic Patent References:
| WO2012134813A1 | 2012-10-04 | |||
| WO2009065226A1 | 2009-05-28 |
Foreign References:
| JP2012532873A | 2012-12-20 | |||
| JP2009525468A | 2009-07-09 | |||
| JP2008256713A | 2008-10-23 |
Other References:
MARIA CELIA JAMUR: "Permeabilization of Cell Membranes", METHODS IN MOLECULAR BIOLOGY, vol. 588, 2010, pages 63 - 66, XP008161767
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
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