Title:
CELLOBIOHYDROLASE HAVING IMPROVED THERMAL STABILITY
Document Type and Number:
WIPO Patent Application WO/2015/182570
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide CBHI having excellent thermal stability. A polypeptide which comprises an amino acid sequence that is produced by adding a mutation (A) and/or a mutation (B) as mentioned below to any one of the amino acid sequence represented by SEQ ID NO: 1, an amino acid sequence having a 85% or more identity to the amino acid sequence represented by SEQ ID NO: 1 and an amino acid sequence produced by substituting, inserting, adding and/or deleting one or several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, and which has a cellobiohydrolase activity and improved thermal stability. The mutation (A): the substitution of at least one amino acid residue selected from the group consisting of S42Q, T43E, K45T, S46A, G47P, N53Q, S54N, T262G, S298P, A426P and V451F. The mutation (B): the substitution of an amino acid domain lying between position-413 to position-416 in the amino acid sequence represented by SEQ ID NO: 1 by the amino acid sequence represented by SEQ ID NO: 2.
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Inventors:
ISHIKAWA KAZUHIKO (JP)
KISHISHITA SEIICHIRO (JP)
NAKABAYASHI MAKOTO (JP)
KAMACHI SAORI (JP)
YANAMOTO TOSHIAKI (JP)
FUJII TATSUYA (JP)
KISHISHITA SEIICHIRO (JP)
NAKABAYASHI MAKOTO (JP)
KAMACHI SAORI (JP)
YANAMOTO TOSHIAKI (JP)
FUJII TATSUYA (JP)
Application Number:
PCT/JP2015/064994
Publication Date:
December 03, 2015
Filing Date:
May 26, 2015
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
International Classes:
C12N9/42; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P19/14
Domestic Patent References:
| WO2012104239A1 | 2012-08-09 |
Foreign References:
| JP2006515506A | 2006-06-01 | |||
| JP2012039967A | 2012-03-01 |
Other References:
VOUTILAINEN S.P. ET AL.: "Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity.", PROTEIN ENG. DES. SEL., vol. 23, no. 2, 2010, pages 69 - 79, XP002642935
GUSAKOV A.V.: "Alternatives to Trichoderma reesei in biofuel production.", TRENDS BIOTECHNOL., vol. 29, no. 9, 2011, pages 419 - 425
FANG X. ET AL.: "Strain improvement of Acremonium cellulolyticus for cellulase production by mutation.", J. BIOSCI. BIOENG., vol. 107, no. 3, 2009, pages 256 - 261, XP026006824, ISSN: 1389-1723
GUSAKOV A.V.: "Alternatives to Trichoderma reesei in biofuel production.", TRENDS BIOTECHNOL., vol. 29, no. 9, 2011, pages 419 - 425
FANG X. ET AL.: "Strain improvement of Acremonium cellulolyticus for cellulase production by mutation.", J. BIOSCI. BIOENG., vol. 107, no. 3, 2009, pages 256 - 261, XP026006824, ISSN: 1389-1723
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
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