Title:
CELLS CAPABLE OF DIFFERENTIATING INTO PLACENTA-CONSTITUTING CELLS, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2020/250438
Kind Code:
A1
Abstract:
Cells that are derived from pluripotent stem cells, capable of differentiating into placenta-constituting cells, and negative for BRDT but positive for TP63. A method for producing the cells capable of differentiating into placenta-constituting cells, said method comprising: (a) a step for culturing mammalian pluripotent stem cells in a medium that contains bone morphogenetic factor 4; and (b) a step for, after step (a), culturing the obtained cells in a medium that contains a growth factor and a ROCK inhibitor.
Inventors:
ARIMA TAKAHIRO (JP)
KOBAYASHI NORIO (JP)
OKAE HIROAKI (JP)
KOBAYASHI NORIO (JP)
OKAE HIROAKI (JP)
Application Number:
PCT/JP2019/023725
Publication Date:
December 17, 2020
Filing Date:
June 14, 2019
Export Citation:
Assignee:
UNIV TOHOKU (JP)
International Classes:
C12N5/073; C12N15/09
Domestic Patent References:
| WO2016143866A1 | 2016-09-15 | |||
| WO2016186078A1 | 2016-11-24 |
Foreign References:
| JP2005520514A | 2005-07-14 | |||
| JPS64832B2 | 1989-01-09 |
Other References:
KRENDL C. ET AL.: "GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotentcy", PROC. NATL. ACAD. SCI. USA, vol. 114, no. 45, 2017, pages E9579 - E9588, XP055771167
OKAE H. ET AL.: "Derivation of Human Trophoblast Stem Cells", CELL STEM CELL, vol. 22, 2018, pages 50 - 63 , e1-e6, XP085331006
GAMAGE T. ET AL.: "Stem cell insights into human trophoblast lineage differentiation", HUMAN REPRODUCTION UPDATE, vol. 23, no. 1, 2017, pages 77 - 103, XP055771173
OKAE H ET AL.: "Derivation of Human Trophoblast Stem Cells", CELL STEM CELL, vol. 22, January 2018 (2018-01-01), pages 50 - 63
GAMAGE TK ET AL.: "Stem cell insights into human trophoblast lineage differentiation", HUM REPROD UPDATE, vol. 23, no. 1, December 2016 (2016-12-01), pages 77 - 103, XP055771173, DOI: 10.1093/humupd/dmw026
"GenBank", Database accession no. NM_001289394.1
"NCBI", Database accession no. ID: 79923
OKAE, H.TOH, H.SATO, T ET AL., CELL STEM CELL, vol. 22, 2018, pages 50 - 63
KRENDL, C.SHAPOSHNIKOV, D.RISHKO, V ET AL., PROC NATL ACAD SCI USA, vol. 114, 2017, pages E9579 - E9588
TRAPNELL, C.ROBERTS, A.GOFF, L ET AL., NAT PROTOC, vol. 7, 2012, pages 562 - 578
MIURA, F.ENOMOTO, Y.DAIRIKI, R ET AL., NUCLEIC ACIDS RES, vol. 40, 2012, pages e136
KRUEGER, F.ANDREWS, S. R., BISMARK: BIOINFORMATICS, vol. 27, 2011, pages 1571 - 1572
HEMBERGER M ET AL., HUM MOL GENET, vol. 19, no. 12, 15 June 2010 (2010-06-15), pages 2456 - 67
LEE CQ ET AL., STEM CELL REP, vol. 6, 2016, pages 257 - 272
See also references of EP 3985103A4
OKAE H. ET AL.: "Derivation of Human Trophoblast Stem Cells", CELL STEM CELL, vol. 22, 2018, pages 50 - 63 , e1-e6, XP085331006
GAMAGE T. ET AL.: "Stem cell insights into human trophoblast lineage differentiation", HUMAN REPRODUCTION UPDATE, vol. 23, no. 1, 2017, pages 77 - 103, XP055771173
OKAE H ET AL.: "Derivation of Human Trophoblast Stem Cells", CELL STEM CELL, vol. 22, January 2018 (2018-01-01), pages 50 - 63
GAMAGE TK ET AL.: "Stem cell insights into human trophoblast lineage differentiation", HUM REPROD UPDATE, vol. 23, no. 1, December 2016 (2016-12-01), pages 77 - 103, XP055771173, DOI: 10.1093/humupd/dmw026
"GenBank", Database accession no. NM_001289394.1
"NCBI", Database accession no. ID: 79923
OKAE, H.TOH, H.SATO, T ET AL., CELL STEM CELL, vol. 22, 2018, pages 50 - 63
KRENDL, C.SHAPOSHNIKOV, D.RISHKO, V ET AL., PROC NATL ACAD SCI USA, vol. 114, 2017, pages E9579 - E9588
TRAPNELL, C.ROBERTS, A.GOFF, L ET AL., NAT PROTOC, vol. 7, 2012, pages 562 - 578
MIURA, F.ENOMOTO, Y.DAIRIKI, R ET AL., NUCLEIC ACIDS RES, vol. 40, 2012, pages e136
KRUEGER, F.ANDREWS, S. R., BISMARK: BIOINFORMATICS, vol. 27, 2011, pages 1571 - 1572
HEMBERGER M ET AL., HUM MOL GENET, vol. 19, no. 12, 15 June 2010 (2010-06-15), pages 2456 - 67
LEE CQ ET AL., STEM CELL REP, vol. 6, 2016, pages 257 - 272
See also references of EP 3985103A4
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
Download PDF: