Title:
CEPHALOSPORIN C ACYLASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2017/143945
Kind Code:
A1
Abstract:
Provided is a cephalosporin C acylase mutant constructed by means of a point mutation method, a gene for encoding said cephalosporin C acylase mutant, a plasmid comprising said gene, a microorganism for transforming said plasmid, and an application for said cephalosporin C acylase or said microorganism in producing 7-aminocephalosporanic acid (7-ACA).
Inventors:
WANG JINGANG (CN)
LIANG YAN (CN)
CHEN SHUMING (CN)
REN LIANG (CN)
LIANG YAN (CN)
CHEN SHUMING (CN)
REN LIANG (CN)
Application Number:
PCT/CN2017/074029
Publication Date:
August 31, 2017
Filing Date:
February 19, 2017
Export Citation:
Assignee:
SHANGHAI GENEWELL BIOTECHNOLOGY CO LTD (CN)
SHANXI XINBAOYUAN PHARMACEUTICAL CO LTD (CN)
SHANXI XINBAOYUAN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N9/80; C12N1/19; C12N1/21; C12N15/55; C12P35/02; C12R1/125; C12R1/19; C12R1/84; C12R1/865
Foreign References:
| CN105543201A | 2016-05-04 | |||
| CN103060298A | 2013-04-24 | |||
| KR20140094150A | 2014-07-30 |
Other References:
XU, XUELI ET AL.: "Study on Mutations of Cephalosporin C Acylase", CHINA BIOTECHNOLOGY, vol. 35, no. 2, 21 January 2015 (2015-01-21), ISSN: 1671-8135
ISHII, Y ET AL.: "High-level Production, Chemical Modification and Site- directed Mutagenesis of a Cephalosporin C Acylase from Pseudomonas Strain N176", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 230, no. 2, 1 June 1995 (1995-06-01), XP001145669, ISSN: 0014-2956
ISHII, Y ET AL.: "High-level Production, Chemical Modification and Site- directed Mutagenesis of a Cephalosporin C Acylase from Pseudomonas Strain N176", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 230, no. 2, 1 June 1995 (1995-06-01), XP001145669, ISSN: 0014-2956
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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