Title:
CHARACTERISTIC PEPTIDE SEGMENT CAPABLE OF DISTINGUISHING DEER HORN GELATIN FROM DEER SKIN GELATIN, AND TEST METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/048049
Kind Code:
A1
Abstract:
A characteristic peptide segment capable of distinguishing a deer horn gelatin from a deer skin gelatin, and a test method therefor. By screening through a large number of experiments, a graph is drawn by using the proportion of a deer horn gelatin in a mixed gelatin as an abscissa and using values of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3 as ordinates, the proportion of the deer horn gelatin has a linear relationship to Apeptide 1/Apeptide 3, and a standard curve equation is established as: y=1.034x+0.0157, R2=0.9908; the proportion of the deer horn gelatin also has a linear relationship to Apeptide 2/Apeptide 3, and a standard curve equation is established as: y=0.7883x+0.0949, R 2=0.9905; the proportion of the deer horn gelatin doped is related to the values of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3, and a mixing proportion of the deer horn gelatin and a deer skin gelatin can be determined according to the ratios of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3. The method is strong in specificity, high in sensitivity and simple in operation, and is capable of distinguishing the deer horn gelatin from the deer skin gelatin, and controlling the quality.
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Inventors:
LIU RUI (CN)
CAI SHUO (CN)
ZHAO KEXUAN (CN)
JIANG MENGTONG (CN)
DUAN JINAO (CN)
CAI SHUO (CN)
ZHAO KEXUAN (CN)
JIANG MENGTONG (CN)
DUAN JINAO (CN)
Application Number:
PCT/CN2020/131799
Publication Date:
March 10, 2022
Filing Date:
November 26, 2020
Export Citation:
Assignee:
UNIV NANJING CHINESE MEDICINE (CN)
International Classes:
G01N30/88; C07K7/00; G01N33/68
Foreign References:
| CN107532196A | 2018-01-02 | |||
| CN109187835A | 2019-01-11 | |||
| CN105273061A | 2016-01-27 | |||
| US20030049715A1 | 2003-03-13 |
Other References:
KATELYN ELIZABETH MASON: "Human Bone Protein and Peptide Output from PEAKS Software", MENDELEY DATA, vol. 1, 4 April 2018 (2018-04-04), DOI: 10.17632/xw4srn74wh.1
ZHANG TAO;SONG SHI-ZHEN;LIU RUI: "Investigation on Identifying Collagen from Colla Corii Bovis Using Nano LC-MS/MS Method", JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE, vol. 36, no. 1, 10 January 2020 (2020-01-10), pages 105 - 109, XP055906667, ISSN: 1672-0482, DOI: 10.14148/j.issn.1672-0482.2020.0105
LIU RUI; HUANG YONG; XU HAOKUN; ZHENG YUNFENG; LIU YANG; HAN SHUYING; YANG MIN; XIE YU; WANG KAIXUAN; DUAN JIN-AO; LI LINGJUN: "A strategy for identifying species-specific peptide biomarkers in deer-hide gelatin using untargeted and targeted mass spectrometry approaches", ANALYTICA CHIMICA ACTA, vol. 1092, 26 September 2019 (2019-09-26), AMSTERDAM, NL , pages 32 - 41, XP085900989, ISSN: 0003-2670, DOI: 10.1016/j.aca.2019.09.064
ZHANG, G. ; LIU, T. ; WANG, Q. ; CHEN, L. ; LEI, J. ; LUO, J. ; MA, G. ; SU, Z.: "Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin", FOOD HYDROCOLLOIDS, vol. 23, no. 7, 1 October 2009 (2009-10-01), NL , pages 2001 - 2007, XP026145900, ISSN: 0268-005X, DOI: 10.1016/j.foodhyd.2009.03.010
YUKI KUMAZAWA;YUKI TAGA;MIHO TAKASHIMA;SHUNJI HATTORI: "A novel LC-MS method using collagen marker peptides for species identification of glue applicable to samples with multiple animal origins", HERITAGE SCIENCE, vol. 6, no. 1, 13 July 2018 (2018-07-13), London, UK , pages 1 - 9, XP021258540, DOI: 10.1186/s40494-018-0209-y
ZHANG TAO;SONG SHI-ZHEN;LIU RUI: "Investigation on Identifying Collagen from Colla Corii Bovis Using Nano LC-MS/MS Method", JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE, vol. 36, no. 1, 10 January 2020 (2020-01-10), pages 105 - 109, XP055906667, ISSN: 1672-0482, DOI: 10.14148/j.issn.1672-0482.2020.0105
LIU RUI; HUANG YONG; XU HAOKUN; ZHENG YUNFENG; LIU YANG; HAN SHUYING; YANG MIN; XIE YU; WANG KAIXUAN; DUAN JIN-AO; LI LINGJUN: "A strategy for identifying species-specific peptide biomarkers in deer-hide gelatin using untargeted and targeted mass spectrometry approaches", ANALYTICA CHIMICA ACTA, vol. 1092, 26 September 2019 (2019-09-26), AMSTERDAM, NL , pages 32 - 41, XP085900989, ISSN: 0003-2670, DOI: 10.1016/j.aca.2019.09.064
ZHANG, G. ; LIU, T. ; WANG, Q. ; CHEN, L. ; LEI, J. ; LUO, J. ; MA, G. ; SU, Z.: "Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin", FOOD HYDROCOLLOIDS, vol. 23, no. 7, 1 October 2009 (2009-10-01), NL , pages 2001 - 2007, XP026145900, ISSN: 0268-005X, DOI: 10.1016/j.foodhyd.2009.03.010
YUKI KUMAZAWA;YUKI TAGA;MIHO TAKASHIMA;SHUNJI HATTORI: "A novel LC-MS method using collagen marker peptides for species identification of glue applicable to samples with multiple animal origins", HERITAGE SCIENCE, vol. 6, no. 1, 13 July 2018 (2018-07-13), London, UK , pages 1 - 9, XP021258540, DOI: 10.1186/s40494-018-0209-y
Attorney, Agent or Firm:
JIANGSU SUNDY LAW FIRM (CN)
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