Title:
CHEMICAL IN VITRO PRESERVATION METHOD FOR PUNICA GRANATUM GERMPLASM
Document Type and Number:
WIPO Patent Application WO/2022/194312
Kind Code:
A2
Abstract:
Disclosed in the present invention is a culture medium for chemical in vitro preservation of Punica granatum germplasm. The culture medium is: WPM+IBA 0.5-0.7 mg·L -1+ agar 6 g·L -1+ sucrose 20-30 g·L -1+ paclobutrazol 5-9 mg·L -1. The present application defines the ingredients and amounts of the culture medium for chemical in vitro preservation of Punica granatum germplasm. The culture medium is used for chemical in vitro preservation of Punica granatum germplasm, which can break the dilemma that varieties or germplasms are lost due to natural disasters in conventional Punica granatum preservation, and break the research state that in vitro germplasm preservation of woody fruit trees is zero due to the problems of browning, extremely easy lignification, etc. The problem of browning occurring in an in vitro preservation process is overcome well, the in vitro preservation time is obviously prolonged, and the survival rate of tissue culture seedlings can still be 100% guaranteed when the seedlings are stored for 180 days.
Inventors:
QIAN JINGJING (CN)
WANG NING (CN)
WANG NING (CN)
Application Number:
PCT/CN2022/104011
Publication Date:
September 22, 2022
Filing Date:
July 06, 2022
Export Citation:
Assignee:
UNIV ANHUI SCIENCE & TECH (CN)
Attorney, Agent or Firm:
BEIJING FINELAND IP FIRM (CN)
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