Title:
CHEMICAL PREPARATION METHOD OF SKELETAL MUSCLE-DERIVED MULTIPOTENT CELLS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/064090
Kind Code:
A2
Abstract:
The present invention provides a chemical preparation method of skeletal muscle-derived multipotent cells comprising: (a) a step for obtaining differentiated cells from mammals; and (b) a step for obtaining multipotent cells by treating the differentiated cells with a dedifferentiation agent after inducing the down-regulation of p21 expression of the differentiated cells. The multipotent cells of the invention enable not only dedifferentiation into a musculogenesis cell population but also the transdifferentiation into an adipogenesis cell population or an osteogenesis cell population. Therefore, the chemical-based approach method of the invention is important in the development of strategies for inducing limb regeneration in mammals. In addition, the invention discloses a new approach method for the development of induced pluripotent stem cells (iPSCs) from completely differentiated refractory tissues.
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Inventors:
WILLIAMS DARREN R (KR)
JUNG DA-WOON (KR)
JUNG DA-WOON (KR)
Application Number:
PCT/KR2011/008473
Publication Date:
May 18, 2012
Filing Date:
November 08, 2011
Export Citation:
Assignee:
KWANGJU INST SCI & TECH (KR)
WILLIAMS DARREN R (KR)
JUNG DA-WOON (KR)
WILLIAMS DARREN R (KR)
JUNG DA-WOON (KR)
International Classes:
C12N5/077; A61K35/34; A61P21/00; C12N5/02
Other References:
CHEN ET AL.: 'Dedifferentiation of lineage-committed cells by a small molecule' JOURNAL OF THE AMERICAN CHEMICAL SOCIETY vol. 126, 19 December 2003, pages 410 - 411
SHAN ET AL.: 'Induction of growth arrest and polycomb gene expression by reversine allows C2C12 cells to be reprogrammed to various differentiated cell types' PROTEOMICS vol. 7, 2007, pages 4303 - 4316
LEE ET AL.: 'Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage' THE JOURNAL OF BIOLOGICAL CHEMISTRY vol. 284, no. 5, 30 January 2009, pages 2891 - 2901
JUNG ET AL.: 'Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia' ACS CHEMICAL BIOLOGY vol. 6, 15 February 2011, pages 553 - 562
SHAN ET AL.: 'Induction of growth arrest and polycomb gene expression by reversine allows C2C12 cells to be reprogrammed to various differentiated cell types' PROTEOMICS vol. 7, 2007, pages 4303 - 4316
LEE ET AL.: 'Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage' THE JOURNAL OF BIOLOGICAL CHEMISTRY vol. 284, no. 5, 30 January 2009, pages 2891 - 2901
JUNG ET AL.: 'Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia' ACS CHEMICAL BIOLOGY vol. 6, 15 February 2011, pages 553 - 562
Attorney, Agent or Firm:
YANG, Boo-Hyun (1659-2 Inhundong Gwanak-gu, Seoul 151-832, KR)
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Claims: