Title:
CHEMICAL PROBE-DEPENDENT EVALUATION OF PROTEIN ACTIVITY AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/104155
Kind Code:
A3
Abstract:
Aspects of the disclosure relate to a method for evaluating two or more target proteins of interest from the same family in a specified functional form, the method comprising: (i) contacting a composition comprising or suspected of comprising the two or more proteins of interest with a molecular construct comprising: a targeting group operatively linked to a retrieval tag; wherein the targeting group specifically binds to the specialized functional form of the two or more target proteins of interest; (ii) contacting the composition with at least two antibody-oligo constructs, wherein at least one of the constructs comprises a first antibody operatively linked to a first oligo and at least a second construct comprises a second antibody operatively linked to a second oligo; wherein the first antibody specifically binds to one of the two or more target proteins of interest and the second antibody specifically binds to the other of the two or more target proteins of interest; (iii) contacting the composition with a second molecular construct comprising a retrieval tag binder operatively linked to a retrieval oligo; (iv) incubating the composition under conditions sufficient for the ligation or annealing of the first oligo to the retrieval oligo when the first and retrieval oligos are in close proximity to each other and ligation or annealing of the second oligo to the retrieval oligo when the second and retrieval oligos are in close proximity to each other; and (v) detecting the ligated or annealed first and retrieval oligo and the ligated or annealed second and retrieval oligo.
Inventors:
MOELLERING RAYMOND E (US)
LI GANG (US)
LI GANG (US)
Application Number:
PCT/US2018/062231
Publication Date:
April 02, 2020
Filing Date:
November 21, 2018
Export Citation:
Assignee:
UNIV CHICAGO (US)
International Classes:
C12N15/10; C40B40/10; C40B70/00
Domestic Patent References:
| WO2012006556A2 | 2012-01-12 | |||
| WO2017192633A1 | 2017-11-09 |
Foreign References:
| US8940497B2 | 2015-01-27 |
Other References:
THOMPSON ET AL.: "SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR", BMC BIOTECHNOLOGY, vol. 15, 60, 26 June 2015 (2015-06-26), pages 1 - 13, XP055548769, DOI: 10.1186/s12896-015-0169-1
NOMURA ET AL.: "Activity-based protein profiling for biochemical pathway discovery in cancer", NAT. REV. CANCER, vol. 10, no. 9, September 2010 (2010-09-01), pages 630 - 638, XP055698185, DOI: 10.1038/nrc2901
LI: "An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells", NATURE COMMUNICATIONS, vol. 8, no. 1775, 24 November 2017 (2017-11-24), XP055548348, DOI: 10.1038/s41467-017-01854-0
SCHEDIN-WEISS, SOPHIA ET AL.: "Visualizing Active Enzyme Complexes Using a Photoreactive Inhibitor for Proximity Ligation – Application on γ-Secretase", PLOS ONE, vol. 8, no. 5, e63962, May 2013 (2013-05-01), pages 1 - 10, XP055318695, DOI: 10.1371/journal.pone.0063962
GREENWOOD ET AL.: "Proximity assays for sensitive quantification of proteins", BIOMOLECULAR DETECTION AND QUANTIFICATION , vol. 4, 20 May 2015 (2015-05-20), pages 10 - 16, XP055364680, DOI: 10.1016/j.bdq.2015.04.002
DONGWOOK KIM, RACHAEL R. JETSON‡ AND CASEY J. KRUSEMARK: " A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe", CHEMICAL COMMUNICATIONS, vol. 53, 2017, pages 9474 - 9477, XP055698212, DOI: 10.1039/c7cc05236g
NOMURA ET AL.: "Activity-based protein profiling for biochemical pathway discovery in cancer", NAT. REV. CANCER, vol. 10, no. 9, September 2010 (2010-09-01), pages 630 - 638, XP055698185, DOI: 10.1038/nrc2901
LI: "An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells", NATURE COMMUNICATIONS, vol. 8, no. 1775, 24 November 2017 (2017-11-24), XP055548348, DOI: 10.1038/s41467-017-01854-0
SCHEDIN-WEISS, SOPHIA ET AL.: "Visualizing Active Enzyme Complexes Using a Photoreactive Inhibitor for Proximity Ligation – Application on γ-Secretase", PLOS ONE, vol. 8, no. 5, e63962, May 2013 (2013-05-01), pages 1 - 10, XP055318695, DOI: 10.1371/journal.pone.0063962
GREENWOOD ET AL.: "Proximity assays for sensitive quantification of proteins", BIOMOLECULAR DETECTION AND QUANTIFICATION , vol. 4, 20 May 2015 (2015-05-20), pages 10 - 16, XP055364680, DOI: 10.1016/j.bdq.2015.04.002
DONGWOOK KIM, RACHAEL R. JETSON‡ AND CASEY J. KRUSEMARK: " A DNA-assisted immunoassay for enzyme activity via a DNA-linked, activity-based probe", CHEMICAL COMMUNICATIONS, vol. 53, 2017, pages 9474 - 9477, XP055698212, DOI: 10.1039/c7cc05236g
Attorney, Agent or Firm:
STELLMAN, Laurie Friesenhahn (US)
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