Title:
CHEMICAL SAMPLE INDEXING FOR HIGH-THROUGHPUT SINGLE-CELL ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2022/188827
Kind Code:
A1
Abstract:
The present invention provides methods and kits for indexing a plurality of samples or analyzing nucleic acids in a plurality of samples, wherein a plurality of cells are associated with the sample-specific-indexing sequence through a coupling agent.
Inventors:
LI JIE (CN)
ZHAO XINLU (CN)
YU WENHAO (CN)
YANG YANG (CN)
ZHU WENQI (CN)
SUN DAFEI (CN)
DING XIUHENG (CN)
ZHAO XINLU (CN)
YU WENHAO (CN)
YANG YANG (CN)
ZHU WENQI (CN)
SUN DAFEI (CN)
DING XIUHENG (CN)
Application Number:
PCT/CN2022/080093
Publication Date:
September 15, 2022
Filing Date:
March 10, 2022
Export Citation:
Assignee:
NANJING UNIVERSITY OF TECHNOLOGY (CN)
SINGLERON NANJING BIOTECHNOLOGIES LTD (CN)
SINGLERON NANJING BIOTECHNOLOGIES LTD (CN)
International Classes:
C12Q1/6869
Foreign References:
| GB2582850A | 2020-10-07 | |||
| CN111378728A | 2020-07-07 | |||
| US20180346969A1 | 2018-12-06 |
Other References:
MCGINNIS CHRISTOPHER S.; PATTERSON DAVID M.; WINKLER JULIANE; CONRAD DANIEL N.; HEIN MARCO Y.; SRIVASTAVA VASUDHA; HU JENNIFER L.;: "MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices", NATURE METHODS, vol. 16, no. 7, 17 June 2019 (2019-06-17), New York, pages 619 - 626, XP036901129, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0433-8
GUO CHUNER, KONG WENJUN, KAMIMOTO KENJI, RIVERA-GONZALEZ GUILLERMO C., YANG XUE, KIRITA YUHEI, MORRIS SAMANTHA A.: "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics", GENOME BIOLOGY, vol. 20, no. 1, 1 December 2019 (2019-12-01), pages 1 - 13, XP055964684, DOI: 10.1186/s13059-019-1699-y
GUO CHUNER, KONG WENJUN, KAMIMOTO KENJI, RIVERA-GONZALEZ GUILLERMO C., YANG XUE, KIRITA YUHEI, MORRIS SAMANTHA A.: "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics", GENOME BIOLOGY, vol. 20, no. 1, 1 December 2019 (2019-12-01), pages 1 - 13, XP055964684, DOI: 10.1186/s13059-019-1699-y
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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