CHEMICAL TAGGING-BASED METHOD FOR MODIFIED NUCLEOSIDE SEQUENCING, ENRICHMENT, AND MEASUREMENT

Title:

CHEMICAL TAGGING-BASED METHOD FOR MODIFIED NUCLEOSIDE SEQUENCING, ENRICHMENT, AND MEASUREMENT

Document Type and Number:

WIPO Patent Application WO/2021/000889

Kind Code:

A1

Abstract:

Provided is a chemical tagging-based method for modified nucleoside sequencing, enrichment, and measurement, comprising reacting a thiol compound with an N-hydroxymethyl chemically modified nucleoside, then using a chemical tag such as biotin to perform tagging, and then enriching and sequencing or measuring. In the method of the present invention, selective chemical tagging is used, and the selectivity of the chemical reaction ensures result specificity and reduces false positive results.

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Inventors:

JIA GUIFANG (CN)
WANG YE (CN)
DONG SHUNQING (CN)

Application Number:

PCT/CN2020/099707

Publication Date:

January 07, 2021

Filing Date:

July 01, 2020

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Assignee:

UNIV BEIJING (CN)

International Classes:

C12Q1/6809

Foreign References:

CN111088252A	2020-05-01
CN108918622A	2018-11-30
CN102731412A	2012-10-17

Other References:

PENG SHUANG , LI WEI , TIAN TIAN , ZHOU XIANG: "Advance in Technologies of Disease Diagnosis Based on Nucleic Acids", WORLD JOURNAL OF COMPLEX MEDICINE, vol. 1, no. 3, 15 September 2015 (2015-09-15), pages 212 - 222, XP055771600, ISSN: 2095-994X, DOI: 10.11966/j.issn.2095-994X.2015.01.03.05
PENG XUE , TAO JIANG , XINGJIA SHEN: "Advance in m~6A Modification and its Regulation of Viral Replication", HEREDITAS, vol. 41, no. 5, 16 April 2019 (2019-04-16), pages 404 - 412, XP055771603, ISSN: 0253-9772, DOI: 10.16288/j.yczz.19-042

Attorney, Agent or Firm:

PATENTSINO IP FIRM (CN)

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