Title:
CHEMICALLY MODIFIED PROTEINS WITH A CARBOHYDRATE MOIETY
Document Type and Number:
WIPO Patent Application WO2000001712
Kind Code:
A3
Abstract:
The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.
Inventors:
JONES J BRYAN
DAVIS BENJAMIN G
DAVIS BENJAMIN G
Application Number:
PCT/US1999/015138
Publication Date:
May 11, 2000
Filing Date:
July 02, 1999
Export Citation:
Assignee:
GENENCOR INT (US)
International Classes:
C12N15/09; C07K14/00; C12N9/54; C12N9/96; C12Q1/25; C12Q1/37; (IPC1-7): C07K1/107; C07K14/00; C12N9/00; C12N9/54; C12N9/96; C12N15/00; C12N15/52; C12N15/57; C12Q1/25; C12Q1/37
Foreign References:
| US5955340A | 1999-09-21 | |||
| US5340735A | 1994-08-23 |
Other References:
BERGLUND P. ET AL: "Chemical Modification of Cysteine Mutants of Subtilisin Bacillus Lentus Can Create Better Catalysts than the Wild-Type Enzyme", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 119, no. 22, 4 June 1997 (1997-06-04), pages 5265 - 5266, XP002063097
SPURA A. ET AL: "Probing the Agonist Domain of the Nicotinic Acetylcholine Receptor by Cysteine Scanninf Mutagenesis Reveals Residues in Proximity to the Alpha-Bungarotoxin Binding Site", BIOCHEMISTRY, vol. 38, no. 16, 20 March 1999 (1999-03-20), pages 4912 - 4921, XP002923569
LO B. ET AL: "Replacement of Ala-166 with Cysteine in the High Affinity Rabbit Sodium/Glucose Transporter Alters Transport Kinetics and Allows Methanethiosulfonate Ethylamine to Inhibit Transporter Function", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 2, 9 January 1998 (1998-01-09), pages 903 - 909, XP002923570
SPURA A. ET AL: "Probing the Agonist Domain of the Nicotinic Acetylcholine Receptor by Cysteine Scanninf Mutagenesis Reveals Residues in Proximity to the Alpha-Bungarotoxin Binding Site", BIOCHEMISTRY, vol. 38, no. 16, 20 March 1999 (1999-03-20), pages 4912 - 4921, XP002923569
LO B. ET AL: "Replacement of Ala-166 with Cysteine in the High Affinity Rabbit Sodium/Glucose Transporter Alters Transport Kinetics and Allows Methanethiosulfonate Ethylamine to Inhibit Transporter Function", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 2, 9 January 1998 (1998-01-09), pages 903 - 909, XP002923570
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