Title:
CHIMERIC ANTIGEN RECEPTOR T CELL SECRETING BISPECIFIC ANTIBODIES, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/104171
Kind Code:
A1
Abstract:
Disclosed are a chimeric antigen receptor T cell secreting bispecific antibodies, a preparation method therefor and use thereof. Experiments show that a chimeric antigen receptor T cell targeting GPC3 and secreting bispecific antibodies has strong killing activity against a GPC3- and CD276-positive tumor cell line and a GPC3-negative CD276-positive tumor cell line. Therefore, the chimeric antigen receptor T cell secreting the bispecific antibodies has great prospects for use in treating hepatocellular carcinoma and the like.
Inventors:
WAN XIAOCHUN (CN)
CAO GUOZHENG (CN)
CAO GUOZHENG (CN)
Application Number:
PCT/CN2022/137695
Publication Date:
June 15, 2023
Filing Date:
December 08, 2022
Export Citation:
Assignee:
SHENZHEN INST ADV TECH (CN)
SHENZHEN INST OF ADV TECH CAS (CN)
SHENZHEN INST OF ADV TECH CAS (CN)
International Classes:
C07K19/00; A61K39/00; A61P1/16; A61P35/00; C12N5/10; C12N15/62; C12N15/867
Foreign References:
| CN114163537A | 2022-03-11 | |||
| CN110545847A | 2019-12-06 | |||
| CN111454358A | 2020-07-28 | |||
| CN109157662A | 2019-01-08 |
Other References:
ZHENG MEIJUN, YU LINGYU, HU JUANJUAN, ZHANG ZONGLIANG, WANG HAIYANG, LU DAN, TANG XIN, HUANG JIANHAN, ZHONG KUNHONG, WANG ZENG, LI: "Efficacy of B7-H3-Redirected BiTE and CAR-T Immunotherapies Against Extranodal Nasal Natural Killer/T Cell Lymphoma", TRANSLATIONAL ONCOLOGY, NEOPLASIA PRESS, UNITED STATES, vol. 13, no. 5, 1 May 2020 (2020-05-01), United States , pages 100770, XP093071719, ISSN: 1936-5233, DOI: 10.1016/j.tranon.2020.100770
MAJZNER ROBBIE G., THERUVATH JOHANNA L., NELLAN ANANDANI, HEITZENEDER SABINE, CUI YONGZHI, MOUNT CHRISTOPHER W., RIETBERG SKYLER P: "CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors", CLINICAL CANCER RESEARCH, ASSOCIATION FOR CANCER RESEARCH, US, vol. 25, no. 8, 15 April 2019 (2019-04-15), US, pages 2560 - 2574, XP055913475, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-18-0432
CAO GUOZHENG, ZHANG GUIZHONG, LIU MAOXUAN, LIU JUNCHEN, WANG QI, ZHU LIFANG, WAN XIAOCHUN: "GPC3-targeted CAR-T cells secreting B7H3-targeted BiTE exhibit potent cytotoxicity activity against hepatocellular carcinoma cell in the in vitro assay", BIOCHEMISTRY AND BIOPHYSICS REPORTS, ELSEVIER, vol. 31, 1 September 2022 (2022-09-01), pages 101324, XP093071737, ISSN: 2405-5808, DOI: 10.1016/j.bbrep.2022.101324
MAJZNER ROBBIE G., THERUVATH JOHANNA L., NELLAN ANANDANI, HEITZENEDER SABINE, CUI YONGZHI, MOUNT CHRISTOPHER W., RIETBERG SKYLER P: "CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors", CLINICAL CANCER RESEARCH, ASSOCIATION FOR CANCER RESEARCH, US, vol. 25, no. 8, 15 April 2019 (2019-04-15), US, pages 2560 - 2574, XP055913475, ISSN: 1078-0432, DOI: 10.1158/1078-0432.CCR-18-0432
CAO GUOZHENG, ZHANG GUIZHONG, LIU MAOXUAN, LIU JUNCHEN, WANG QI, ZHU LIFANG, WAN XIAOCHUN: "GPC3-targeted CAR-T cells secreting B7H3-targeted BiTE exhibit potent cytotoxicity activity against hepatocellular carcinoma cell in the in vitro assay", BIOCHEMISTRY AND BIOPHYSICS REPORTS, ELSEVIER, vol. 31, 1 September 2022 (2022-09-01), pages 101324, XP093071737, ISSN: 2405-5808, DOI: 10.1016/j.bbrep.2022.101324
Attorney, Agent or Firm:
BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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