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Patent Searching and Data


Title:
CHIP FOR CELL CLASSIFICATION
Document Type and Number:
WIPO Patent Application WO/2019/078277
Kind Code:
A1
Abstract:
This chip (20) is equipped with micro flow channel units for hydro-dynamically classifying cells in a blood sample. In each of the micro flow channel units, a liquid flowing out from a sub flow channel into a main flow channel squeezes cells flowing through the main flow channel toward the side of a removal flow channel and a recovery flow channel. When a fluid containing non-nucleated red blood cells among the cells being squeezed enters the removal flow channel, the non-nucleated red blood cells are removed from the blood sample. Micro flow channel units having the same pattern are repeatedly stacked on one another in the height direction. In the respective micro flow channel units, main flow channel entrances (51), sub flow channel entrances (61), removal flow channel exits (54a), recovery flow channel exits (54d), and main flow channel exits (55) are respectively connected to columnar flow channels (22, 23, 24a, 24d and 25) that penetrate respective layers (L01-L10) in a cross-cutting manner, so as to be consolidated by the respective columnar flow channels (22, 23, 24a, 24d and 25).

Inventors:
KUBO TOMOHIRO (JP)
AYANO MADOKA (JP)
Application Number:
PCT/JP2018/038728
Publication Date:
April 25, 2019
Filing Date:
October 17, 2018
Export Citation:
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Assignee:
TL GENOMICS INC (JP)
International Classes:
G01N33/48; C12M1/34; C12N5/078; C12Q1/68; G01N33/49; G01N37/00
Foreign References:
JP2013017429A2013-01-31
JPH11508182A1999-07-21
JPS5311356B21978-04-20
JP2009511001A2009-03-19
JP2005205387A2005-08-04
JP2007175684A2007-07-12
JP4091123B22008-05-28
JP2007530629A2007-11-01
JPS5857537B21983-12-20
JPS538834B21978-04-01
Other References:
HUANG, R. ET AL.: "A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women", PRENATAL DIAGNOSIS, vol. 28, 2008, pages 892 - 899, XP055197085, DOI: doi:10.1002/pd.2079
MIZUNO, M. ET AL.: "Magnetophoresis- Integrated Hydrodynamic Filtration System for Size- and Surface Marker-Based Two-Dimensional Cell Sorting", ANALYTICAL CHEMISTRY, vol. 85, June 2013 (2013-06-01), pages 7666 - 7673, XP055369554, DOI: doi:10.1021/ac303336f
SAJEESH, P. ET AL.: "Characterization and sorting of cells based on stiffness contrast in a microfluidic channel", RSC ADVANCES, vol. 6, no. 78, 29 July 2016 (2016-07-29), pages 74704 - 74714, XP055597930, ISSN: 2046-2069, DOI: 10.1039/C6RA09099K
TAIZAN KAMIDENAGAYOSHI UMEHARAHARUHIKO SAGO: "New Trials For Efficient Erythroblast Isolation From Maternal Blood", TOKYO JIKEIKAI MEDICAL JOURNAL, vol. 130, no. 1, 2015, pages 11 - 17
See also references of EP 3699587A4
Attorney, Agent or Firm:
IEIRI Takeshi (JP)
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