1. Technical Field

The invention is in the field of chromatography. In particular, the invention relates to improvements in the separation of chiral compounds by micellar electrokinetic capillary chromatography.

2. Background .Art

Capillary electrophoresis ("CE") is a well known separation technique that is of increasing interest to those concerned with separations. It is a modification of electrophoresis, typically practiced in a thin glass capillary instead of on a 2-dimensional surface such as paper or in a gel. This technique offers the benefits of high efficiency and resolution, rapid separations, the ability to analyze small sample amounts, and a desirable simplicity from the point of view of the apparatus required when compared to competing analytical techniques such as gel electrophoresis, gas chromatography, and liquid chromatography. .As in all separation systems high resolution is the end objective and as in other systems resolution is a function of efficiency (theoretical plates) and selectivity (Weinberger, R. "Practical Capillary Electrophoresis", Academic Press, San Diego, CA 1993).

The benefits of capillary electrophoresis derive to a large extent from the use of narrow diameter capillary tubes, which permit efficient removal of the heat generated in the separation process. This heat removal prevents convective mixing which would degrade the separating power. The narrow diameter tubes also allow high voltages to be used to generate the electric field in the capillary while limiting current flow and hence heat generation.

A CE separation begins by filling the capillary with a supporting electrolyte. Next, a small amount of sample is injected into one end of the capillary. Typical sample injection volumes range from 1-20 nanoliters. .After sample injection, a high voltage is applied to the capillary and the sample components are separated on the basis of different charge/mass ratios. A capillary electrophoretic separation can also be augmented with a bulk fluid flow, called electroosmotic flow. If present, it moves all components through the capillary tube at the same rate, and generally does not contribute to the resolution of different sample components. Eventually, the sample components move through an appropriate detector, such as a UV detector. This can provide detection and quantitation of each separated sample zone.

A micelle is a colloidal particle formed from surfactant molecules. The practice of capillary electrophoresis in the presence of micelles is commonly referred to as micellar electrokinetic chromatography (Terabe, S., Otsuka, K., Ichikawa, K., Tsuchiya, A. and Ando, T. Analytical Chemistry, 1984, (56) 111-113; Terabe, S., Otsuka, K., and Ando, T. Analytical Chemistry, 1985, (57) 834-841 ). This term will be used to refer to both micellar electrophoretic separations,

(separations where the electroosmotic flow is negligible), and micellar electrokinetic separations (separations where d e electroosmotic flow impacts the separation time). The equations for retention and resolution in MEKC are shown as equations 1 and 2 (Terabe, S., et al., supra).

tr - t k = 0

mc- equation 1

where t- = retention time of the solute to = retention time of solute in the absence of micelles t _jjjf . = micelle retention time and k is the solute's capacity factor.

equation 2 where N = efficiency (theoretical plates) α = selectivity.

Equation 3 is the resolution equation for HPLC, with all terms as previously defined.

Rs = equation 3

The resolution equation for MEKC is very similar to the resolution equation for HPLC. In fact, as t _jjjj . approaches infinity, the equations become identical (equation 3). Considering die case where t _jjy . or to equals infinity, one can easily see the difference between HPLC and MEKC. The practical difference between HPLC and MEKC is related to the efficiency term of die resolution equation. In HPLC a typical value of N (theoretical plates) is 5000 while a typical value of N for MEKC is 100,000. In quantitative terms a widely accepted goal for resolution of two peaks is a value of 1.5. Assuming k' = 1, then die resolution requirement of 1.5 requires an alpha value of 1.20 for the HPLC case. However, the much higher efficiency of MEKC determines that a much smaller alpha of 1.04 achieves the same resolution of 1.5. Thus, if the small alphas associated with

many partial HPLC separations could be achieved in an MEKC system, useful resolutions would result. (Note that because resolution depends on die term ((α -1 )/ α ), an alpha of 1.04 provides twice die resolution of an alpha of 1.02.)

Chiral separations have been accomplished using a variety of techniques. Over die last thirty years investigators have shown tiiat chiral separations are possible using gas chromatography (GC) and liquid chromatography (LC) (Zief, M. and Crane, L.J., Editors, "Chromatographic Chiral Separations" Marcel Dekker, Inc., New York.Basel, 1988), gel electrophoresis (Barton, J. K, J. Biomolecular Structure and Dynamics, 1983, (1) 621-632), paper electrophoresis (Fanali, S., Cardaci V., Ossicini,L., J. Chromatogr. 1983, (265) 131-135) and capillary electrophoresis (CE) (Snopek, J., Jelinek, I. and Smolkova-Keulemansova, E. Journal of Chromatography, 1992, (609) 1-17). These separations are based on die ability of the enantiomers of die sample to differentially interact widi a chiral phase tiiat is part of the separation system. The chiral phase can be embodied in a variety of ways. In chromatography, the chiral phase is conventionally part of die stationary phase, or column. In botii GC and LC, a wide variety of chiral columns are available. The adsoφtion of die enantiomers by die stationary phase is die sum of both achiral and chiral interactions. The achiral interactions might include ionic, hydrogen bonding, and hydrophobic adsorption. The chiral interactions are derived from die spatial relationship of die achiral interactions. The energy difference contributed by tiiis chiral interaction is die basis for the chiral separation. The efficiency of d e current generation of chiral chromatographic systems is generally low, tiius the difference in die free energy of the interaction between die chiral modifier and die enantiomers must be relatively large in order to gain adequate resolution. This large energy difference requirement contributes to the low efficiency of many chiral HPLC systems (5000 to 10000 plates), and die tailing peaks observed on many chiral columns. This large energy difference requirement also prevents chiral HPLC columns from being of general use. Currentiy, chiral HPLC columns are selective for small classes of compounds, so more dian fifty chiral phases have been commercialized. In tiiis environment, metiiod development is highly empirical and very tedious. Chiral separations per se have little novelty today, die challenge being to create systems which - separate larger classes of enantiomers or provide easier method development. In conventional gel electrophoresis, a chiral phase may be created by adding a chiral modifier to die gel buffer, or by covalent attachment of die modifier to die gel matrix (Barton, J. K., J. Biomolecular Structure and Dynamics, 1983, (1) 621-632). The separation occurs, as in chromatography, through a differential interaction of die individual enantiomers with d e chiral phase. In gel electrophoresis, this results in a change in die overall electrophoretic mobilities of die two chiral molecules. As the two enantiomers move tiirough die gel at different velocities, the separation is effected. Because die presence of d e gel severely reduces bulk fluid movement, die

osmotic flow tiiat is commonly found in electrophoretic separations is minimized. Therefore, the final chiral separation is due largely to the change in electrophoretic mobility of the two enantiomers.

One of die major potential advantages of CE for chiral separations is die relatively high efficiency of die technique. This high efficiency permits die use of chiral modifiers tiiat create only a small difference in free energies between the two enantiomers and die modifier. In CE die chiral phase is generally added to die supporting electrolyte. As in conventional gel electrophoresis, the two enantiomers bind differentially to die chiral modifier, resulting in a change in die electrophoretic mobilities. The resulting difference between die mobilities of die two enantiomers results in tiieir separation. Although electroosmotic flow may sometimes be present in CE separations, it generally does not contribute significandy to die quality of the chiral separation. Chiral CE separations were first disclosed in 1985 (Zare, R.N. and Gassmann, E. U.S. Patent 4,675,300, June 23, 1987), and a few samples have now been analyzed. However, die present art does not allow useful separations of a wide variety of compounds - it suffers from the same hmitations as chiral HPLC separations; tedious mediod development and narrow selectivity. All separations have been accomplished dirough die addition of a chiral modifier to die supporting electrolyte. The kind of chiral modifiers used fall into tiiree categories. The first is die use of amino acid/metal complexes (Zare, R.N. and Gassmann, E. U.S. Patent 4,675,300, June 23, 1987; Gassmann, E., Kuo, J.E. and Zare, R.N. Science, 1985, (230) 813-814; Gozel, P., Gassmann, E., Mchelsen, H. and Zare, R.N. Analytical Chemistry, 1987, (59) 44-49). This type of complex is highly water soluble, and works well for chiral amino acid separations. This system has previously been shown to work using LC as well as gel electrophoresis for the same sample set. The second category of chiral modifiers is a class of carbohydrates called cyclodextrins (Guttmann, A., Paulus, A., Cohen, A.S., Grinberg, N. and Karger, B.L. Journal of Chromatography, 1988, (448) 41-53; Fanali, S. Journal of Chromatography, 1989, (474) 441-446). These modifiers are also highly water soluble, and are also employed extensively in LC. The tiiird category of chiral modifiers are chiral detergents (Cohen, A.S., Paulus, A. and Karger, B.L. Chromatographia, 1987, (24) 15-24; Dobashi, A., Ono, T., Hara, S. and Yamaguchi, J. Analytical Chemistry, 1989, (61) 1984-1986; Terabe, S., Shibata, M. and Miyashita, Y. Journal of Chromatography, 1989, (480) 403-411). The chiral detergent (S)-N,N-didecylalanine has been used witii sodium dodecyl sulfate micelles and copper complexation to effect chiral separations (Cohen, A.S., Paulus, A. and Karger, B.L. Chromatographia, 1987, (24) 15-24). The chiral separation mechanism is based on ligand exchange as in die system reported by Zare, supra. The use of chiral detergents in die supporting electrolyte above their critical micelle concentration is art tiiat has been practiced botii witii die use of sodium dodecanoylvaline (Dobashi, A., Ono, T., Hara, S. and Yamaguchi, J. Analytical

Chemistry, 1989, (61) 1984-1986; Dobashi, A., Ono, T., Hara, S. and Yamaguchi, J. Journal of

Chromatography, 1989, (480) 413-420), as well as with the use of bile salts (Terabe, S., Shibata, M. and Miyashita, Y. Journal of Chromatography, 1989, (480) 403-411). The micelle formed from these detergents is a structure composed of many individual detergent molecules. The inside of die micelle is generally a hydrophobic environment, much like die hydrophobic layer of a reversed phase chromatographic packing. The outside of d e micelle presents hydrophilic groups tiiat are often ionic, resulting in water solubility of the micelle particle. Somewhere in the surfactant tiiere is a chiral center or centers which confer chirality to the micellar environment.

The present art of CE and in particular MEKC is similar to die HPLC art in tiiat none of die systems have been shown to have broad applicability. Zare et al. have shown amino acid separations based on copper complexes which are well known from botii chiral electrophoretic separations and chiral HPLC separations and are not useful for most otiier types of compounds. Cyclodextrin separations are restricted to molecules tiiat can interact witii die cyclodextrin cavity. Chiral MEKC separations have not been shown for acidic compounds nor for a range of compounds of a given class. Sodium dodecanoylvaline, as reported by Hara, has allowed chiral separations of neutral amino acid derivatives as well as a few other neutral compounds. Successful separation of organic bases or acids has not been demonstrated with tiiis chiral surfactant. Bile salts have only been able to show chiral separation of analytes with very rigid structures containing fused ring systems.

3. Disclosure of die Invention

The invention provides a set of chiral surfactants and metiiods for tiieir use, tiiat are used in conjunction with capillary electrophoresis hardware for die purpose of effecting chiral separations. This invention provides a simple, efficient, and high speed metiiodology witii good resolution, high sensitivity, and easy methods development for effectively separating a wide variety of chiral compounds. As such, tiiis invention may, for the first time, provide a universal metiiod of separating any chiral compound without having to resort to specialized stationary phase columns for difficult separations.

In accordance with the preferred embodiment of tiiis invention, chiral surfactants are defined as being composed of die following parts. First, a chiral selector. This may be any amino acid, or derivative tiiereof, or an amino alcohol or tartaric acid derivative. It functions to interact with chiral molecules. Second, a hydrophilic head group that is linked to the chiral selector that confers overall solubility to the micelle, and may also potentiate (increase) enantioselectivity. Third, a "linker" group that links die tail to die chiral selector. This "linker" group is also used to potentiate the chiral selectivity of the chiral selector. Fourth,' a hydrophobic carbon chain or tail. The chain lengtii may vary, and may exist in hnear, branched, substituted, or otiier forms, so long

as it exhibits hydrophobicity and/or functions to partition the analyte into the chiral surfactant molecule. The tail may also potentiate the chiral selectivity of the chiral selector.

The invention is directed to a chiral surfactant having the general formula:

n wherein

R\ = C4-C1 g linear alkyls, C4-C]g branched alkyls, C4-C1 g halogen-substituted linear aUcyls, C4-C1 g polyether hydrocarbons, C4-C g alkyls having a chiral center, all alkyls optionally being unsaturated, and cholesterolic hydrocarbons;

A = NH, CO, SO or SO ₂ ; Y = O, NH or CH ₂ ; X = CO, O or NH; C = carbon; a ≠b, and n may be from 1 to 5;

Z = COO ^" , SO ₄ ^" , SO3 ^" , PO3 ^" , PO ₄ ^" , N ' ₃ ⁺ , PR'3 ⁺ , -OH, polyethers, zwitterions, polyalcohols;

R = H or C1-C4 linear or branched hydrocarbon, optionally halogenated, optionally unsaturated; and when R] is a linear hydrocarbon, and Y = CH2, A= CO, X = NH, Z=COO" and a or b = H but a≠b, tiien a or b ≠ methyl or isopropyl. The invention is also directed to a chiral surfactant having the general formula:

₂ RO H Y-A-X-C-C-Z H OR 3

wherein Rj = C4-C1 g hnear alkyls, C4-C1 g branched alkyls, C4-C _] g halogen-substituted hnear alkyls, C4-Cj polyether hydrocarbons, C4-Cjg alkyls having a chiral center, all alkyls optionally being unsaturated, and cholesterolic hydrocarbons;

R2, R3 = H or C1-C8 linear or branched alkyl or alkenyl hydrocarbons;

Y = CH ₂ ; A = NH; X = CO; C = carbon;

, + , + Z = COO , SO4 , SO3 , PO3 , PO4 , NR 3 , PR 3 , -OH, polyethers, zwitterions, polyalcohols; and

R = H or Ci -C4 hnear or branched hydrocarbon, optionally halogenated, optionally unsaturated. AAISO included in die invention is a chiral surfactant having the general formula:

wherein

R- = C4-C1 g hnear alkyls, C4~C] branched alkyls, C4-C]g halogen-substituted hnear alkyls, c _j -C _j polyether hydrocarbons, C4-C _j alkyls having a chiral center, all alkyls optionally being unsaturated, and cholesterolic hydrocarbons;

R = H or C|-C4 hnear or branched hydrocarbon, optionally halogenated, optionally unsaturated;

Y = O, NH or CH ₂ ; A = CO, SO or S0 ₂ ; X = O, NH; a ≠ b, and n may be from 1 to 5; and , + , +

Z = COO , SO4 , SO3 , PO3 , P0 ₄ , NR 3 , PR 3 , -OH, polyethers, zwitterions, or polyalcohols.

The invention is also directed to a chiral surfactant comprising: a chiral selector, said chiral selector having at least one chiral center;

a hydrophilic head group bonded to said chiral selector, which in combination witii said chiral selector potentiates the chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound;

a linker bonded to said chiral selector which in combination with said chiral selector potentiates die chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound; and

a hydrophobic tail bonded to said linker, which in combination with said chiral selector potentiates the chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound.

The head group is selected from the group consisting of quaternary ammoniums, ammonium salts, carboxylates, alcohols, sulfates, sulfonic acids, polyalcohols, zwitterions, and the

respective salts thereof. Representative sulfate head groups are included in: (S)-2-[( 1- oxododecoxy).amino]-3-memyl-l-sulfboxybutane. Carboxylate head groups are included in: (S)- N-dodecoxycarbonylvahne; (R)-N-dodecoxycarbonylvaline; (S)-N-dodecoxycarbonyl-tert-leucine; (S)-N-tetradecoxycarbonylvahne; and (S)-N-dodecoxycarbonylphenylglycine. Alcohol head groups are included in: (S)-N-dodecanoylvalinol; (S)-N-dodecylaminocarbonylvaUnol; and (S)-N- dodecoxycarbonylvahnol.

Chiral selectors are selected from the group consisting of amino acids, amino alcohols, and tartrates, and the salts thereof. Some representative compounds of this invention that demonstrate amino acids as chiral selectors are: (S)-N-Dodecoxycarbonylserine, (S)-N- Dodecoxycarbonylalanine, (S)-N-Dodecoxycarbonylleucine, and (S)-N-Dodecoxycarbonylproline. Some representative compounds of this invention that demonstrate amino alcohols as chiral selectors are: (S)-N-dodecanoylvalinol; (lS,2R)-N-dodecanoylephedrine; (lS,2R)-N-amino-l- phenyl-l,3-propanedioldodecanamide; and (S)-2-[(Oxododecyl)amino]-3-methyl-l-sulfooxybutane. Some representative compounds of this invention that demonstrate tartaric acid derivatives as chiral selectors are: (R,R)-N-dodecyl-0,0'-diacetyltartaric acid monoamide; (R,R)-N-dodecyltaιtaric acid monoamide; (R,R)-N-decyl-0,0'-diacetyltartaric acid monoamide; and (R,R)-N-decyltartaric acid monoamide.

This invention is also directed to chiral surfactants wherein die linker is selected from the group consisting of amides, carbamates, sulphonamides, and ureas. Carbamate-containing linkers include: (S)-N-dodecoxycarbonylvaline; (R)-N-dodecoxycarbonylvaline; (S)-N-dodecoxycarbonyl- tert-leucine; (S)-N-tetradecoxycarbonylvahne; and (S)-N-dodecoxycarbonylphenylglycine. Sulphonamide-containing linkers include: (S)-N-dodecylsulfonylvaline. Urea-containing linkers include: (S)-N- dodecylaminocarbonylvahne; and (S)-N-dodecylaminocarbonylvalinol. Amide- containing inkers include (R,R)-N-Decyltartaric acid monoamide, (S)-2-[(l-oxododecyl)amino]-3- metiiyl- 1 -sulfoxybutane, and N-Dodecyl-(S)-leucinamide hydrochloride.

This invention also includes chiral surfactants wherein the tail is selected from the group consisting of hnear alkyls, substituted hnear alkyls, hnear alkenyls, substituted hnear alkenyls, cholesterolic, and polyether hydrocarbons, including C4-C1 g hnear alkyls, C4-C1 g branched alkyls, C4-C _j g halogen-substituted hnear alkyls, C4-C1 g polyedier hydrocarbons, C4-C _] g alkyls having a chiral center, all alkyls optionally being unsaturated, and cholesterolic hydrocarbons. Tails having different lengtiis are demonstrated by (S)-N-octanoylvalinol, (S)-N- octoxycarbonylvaline; (S)-N-dodecoxycarbonylvaline; and (S)-N-pentadecafluorooctanoylvahne, and (S)-N-Tetradecoxycarbonylvaline. Halogen-substituted tails are demonstrated by fluorocarbon tails, particularly (S)-N-Pentadecafluorooctanoylvaline. Polyedier hydrocarbon tails are also demonstrated herein, and include (S)-N-dodecylpolyoxyethylene(4)oxycarbonylvahne.

Cholesterolic tails are also demonstrated herein and include (S)-N-cholesteroxycarbonylvalinol.

The invention also includes a kit for separating chiral compounds into tiieir constituent enantiomers comprising, alone or in combination, a chiral surfactant of the present invention in combination witii: an electrolyte effective for chiral capillary electrophoresis; a channel capable of containing the electrolyte; a power supply capable of generating a field strength of at least about 10 V/cm to about 1 kV/cm; at least one anode and cadiode electrically connected to die opposite ends of the channel; and a detector for sensing the presence of the separated enantiomers.

The invention also includes a kit for separating chiral compounds into tiieir constituent enantiomers, compartmentalized to receive in close confinement one or more containers, which comprises in combination a first container comprising any chiral surfactant of this invention. The kit may also comprise at least a second container comprising a different chiral surfactant of this invention, whereby said differing chiral surfactants are admixed to form a mixed chiral surfactant formulation. The kit may also comprise at least an additional container containing an achiral surfactant.

The invention is also direceted to a metiiod for separating a chiral compound into its constituent enantiomers, comprising the step of contacting said chiral compound witii an effective amount of a micellar chiral surfactant under electrophoretic capillary chromatographic conditions, said chiral surfactant comprising:

a chiral selector, said chiral selector having at least one chiral center;

a hydrophilic head group bonded to said chiral selector, which in combination with said chiral selector potentiates die chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound;

a linker bonded to said chiral selector which in combination with said chiral selector potentiates the chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound; and

a hydrophobic tail bonded to said linker, which in combination with said chiral selector potentiates die chiral selectivity of said chiral surfactant sufficientiy to effect substantial separation of a chiral compound. This metiiod also applies where die chiral surfactant is present at or above its critical micellar concentration in substantially aqueous solution, and wherein the electrophoretic conditions include substantial electroosmotic flow. The invention is also direceted to a novel method for analyzing the dipeptide

Aspartame, comprising the steps of injecting a sample of aspartame into a micellar electrokinetic capillary electrophoresis apparatus, said apparatus having an electrolyte

αmtaining a chiral surfactant of this invention, separating the enantiomers of aspartame, and then detecting the separated enantiomers. This invention provides a high-speed method for process testing in the food and beverage market

It is an object of this invention to provide a broad chiral surfactant selectivity which confers the abihty to do separations of a wide variety of compounds witii a single or small number of chiral surfactants.

It is another object to provide increased chiral surfactant types having solubility over a wide pH and concentration range, which further allows flexibility and broader selectivity in developing chiral separations. It as yet anodier object to provide the abihty to modulate enantioselectivity by altering the linking group, the hydrophilic head group, and die hydrophobic tail group.

It is still another object to provide better UV transmissibihty, which allows better sensitivity and linearity of quantitation.

Other aspects, advantages and objectives of the present invention will become apparent from the following detailed description taken in conjunction with the accompanying drawings.

4. Brief Description of the Drawings

FIG. 1 is a block diagram of a typical capillary electrophoresis system.

FIG. 2(A) shows the key elements in block diagram format of the chiral surfactants described in this invention, which include die tail, linker, chiral selector and head.

FIG. 2(B) is a chemical structure of a typical chiral surfactant corresponding to die block diagram of Fig. 2(A).

FIG. 3 lists die structures of the surfactants which have been prepared and evaluated for enantioselectivity for this invention. FIG. 4 shows the idealized structure of a micelle, with sodium dodecyl sulfate used as an example.

FIG. 5 is a vector diagram of micelle, electroosmotic and analyte mobilities in a typical low electroosmotic flow chiral MEKC separation.

FIG. 6 shows the effect that increasing the concentration of (S)-N-dodecoxycarbonylvaline from 25 mM to 100 mM has on the chiral separation of atenolol.

FIG. 7 shows how the concentration of (S)-N-dodecoxycarbonylvaline can be lowered from 25 mM to 5 mM in order to effect chiral separation of the hydrophobic analyte propranolol.

FIG. 8 is a chiral separation of die hydrophobic analyte oxprenolol obtained witii 25 mM (S)-N-dodecylpolyoxyethylene(4)oxycarbonylvahne. FIG. 9 is a chiral separation of bupivacaine obtained with 50 mM (S)-N- pentadecafluorooctanoylvaline.

FIG. 10 is a chiral separation of the hydrophobic analyte propranolol with 25 mM (S)-N- dodecoxycarbonylvahne and 30% acetonitrile.

FIG. 11 shows the separation of several PTΗ-amino acids using valine, phenylglycine, prohne and serine based chiral surfactants. FIG. 12(A) shows chiral separation of PTH-tryptophan obtained with 25 mM (S)-N- dodecanoylvahnol/25 mM sodium dodecyl sulfate.

FIG. 12(B) shows chiral separation of terbutaline obtained witii 25 mM (R,R)-N- dodecyltartaric acid monoamide, sodium salt/25 mM sodium dodecyl sulfate.

FIG. 12(C) shows chiral separation of bupivacaine obtained witii 15 mM (IS, 2S)-N- amino-l-phenyl-l,3-propanediol dodecanamide/15 mM sodium dodecyl sulfate.

FIG. 13(A) shows chiral separation of metoprolol obtained with 25 mM (S)-N- dodecylsulfonylvaline (sulfonamide linker).

FIG. 13(B) shows chiral separation of metoprolol obtained with 25 mM (S)-N- dodecylaminocarbonylvaline (urea linker). FIG. 13(C) shows chiral separation of metoprolol obtained witii 25 mM (S)-N- dodecoxycarbonylvaline (carbamate linker).

FIG. 14(A) shows chiral separation of ketamine with an alpha of 1.01 using 25 mM (S)- N-dodecoxycarbonylvahne (carbamate linker).

FIG. 14(B) shows chiral separation of ketamine with an alpha of 1.04 using 25 mM (S)-N- dodecylaminocarbonylvaline (urea linker).

FIG. 15 provides structures of proglumide and CBZ-tryptophan.

FIG. 16(A) shows chiral separation of proglumide obtained at pH 3.0 with 25 mM (S 2- [( 1 -oxododecyl)amino]-3-methyl- 1 -sulfooxybutane.

FIG. 16(B) shows chiral separation of CBZ-tryptophan obtained at pH 3.0 with 25 mM (S)-2-[(l-oxododecyl)amino]-3-metiiyl-l-sulfooxybutane.

FIG. 17 provides the reaction sequence of a primary or secondary amine to provide N-6- quinolylaminocarbonyl-tagged derivatives.

FIG. 18 shows the chiral separation of racemic N-6-quinolylaminocarbonylarginine using 100 mM (S)-N-dodecoxycarbonylvaline. FIG. 19(A) shows the chiral separation of benzoin obtained at an electroosmotic mobihty of 1.4 x 10-4 cm2 Vs (pH 4.0) with 20 mM (S)-2-[(l-oxododecyl)amino]-3-methyl-l- sulfooxybutane.

FIG. 19(B) shows the chiral separation of benzoin obtained at an electroosmotic mobihty of 3.19 x 10-4 cm2 Vs (pH 6.0) with 20 mM (S>2-[(l-oxododecyl)amino]-3-methyl-l- sulfooxybutane

FIG. 20(A) shows chiral separation of ephedrine obtained in a coated capillary at pH 7 with an electroosmotic mobihty of 0.7 x 10-4 cm2 Vs using 25 mM (S)-N- dodecoxycarbonylvaline.

FIG. 20(B) shows chiral separation of ephedrine obtained in a uncoated capillary at pH 7 with an electroosmotic mobihty of 5.7 x 10-4 cm2/Vs using 25 mM (S)-N- dodecoxycarbonylvaline.

FIG. 21(A)-(C) are chromatographs of the separation of ephedrine in urine. FIGS. 22(A (B) are chromatograms showing the separation of N-benzoyl-DL-alanine with (S)-2-[(l-oxododecyl)anuno]-4-methyl-l-sulfooxypentane ( Fig. 3bd, C12-amide- leucinol-sulfate); Figure 22(B) shows the separation of the same compound on N-dodecyl- (S)-leucinamidehydrodιloride (Fig. 3bl, C12-amide-leudne-ammonium salt).

FIG. 23 is a chromatogram of the separation of artφhetamine using the chiral surfactant (S)-N-dodecoxycarbonylleucine (Fig. 3ak).

FIGS. 24(A)-(B) are chromatograms of the separation of amphetamine using the chiral surfactants (S)-N-dodecoxycarbcttylcyclohexylalanine (Fig. 3ao, Figure 24A), and (R,R)-N-decyltartaric acid monoamide, sodium salt (Fig. 3ag, Fig. 24B).

FIG. 25 is a chromatogram showing the separation of the anionic compound carboxybenzoyl-DL-alanine using 25 mM N-dodecyl-(S)-prolinamide hydrochloride (Fig. 3bn) atpH 3.0. FIGS. 26(A)-(B) are chromatograms showing, in Fig. 26A, the separation of nicotine obtained with 100 mM (S)-dodecylammocarbonylvaline (Fig. 3g, C12 tail) at pH 8.0, and in Fig. 26B, with 100 mM (S)-decylaminocarbonylvaline (Fig. 3ay, CIO tail) at pH 7.5.

FIG. 27 is a chromatogram showing the separation of all four stereoisomers of aspartame using 25 mM (S)- 2-[(l-oxodode(^l)-amino]-3-methyl-l -sulfooxybutane (Fig. 3m) at pH 3.5.

5. Modes for Carrying Out the Invention

The invention is directed to chiral surfactants, methods of making and using them, apparatus and kits for performing chiral separations using the novel compounds of this invention. In addition to die abihty to form micelles, the chiral surfactants of this invention also possess several other characteristics. First, they show enantioselectivity, or alpha, toward enantiomeric mixtures. Second, they show the abihty to partition compounds of interest. Third, tiiey show high efficiency, or plates, when used in an MEKC system. Fourth, they have desirable properties witii

regard to die detection mode. Finally, their contribution to the conductivity of the MEKC buffer should be minimized. The figures and examples described below further illustrate these characteristics.

Figure 1 shows a block diagram of a CE system. The system includes a fused silica capillary 10 witii an inside diameter having a range of about 5μm to 500μm, and an outside diameter having a range of about lOOμm to lOOOμm and a lengtii having a range of about 5 cm to 200 cm. The capillary includes an inlet end 20 immersed in an reservoir 30 containing electrolyte and an outlet end 40 immersed in an electrolyte 50. Both inlet and outiet electrolyte reservoirs as well as the capillary are filled with electrolyte which is composed of a supporting buffer that contains a chiral surfactant. The capillary is also filled witii the electrolyte. Contacting the reservoirs are separate electrodes 60 and 70 that are connected to die output terminals 80 and 90 of a high voltage power supply 100. The electrical circuit is completed from the input electrode dirough the filled capillary to the outlet end of die capillary and to the high voltage electrode tiiat is immersed in die outiet reservoir. The high voltage power supply output is set by a controller 110, and the separation monitored by a UV Vis detector 120.

The structural components of the chiral surfactants of the present invention are shown in Figure 2. These surfactants can be divided into four structural units. Starting at the left, the first is the hydrophobic tail, which helps determine the abihty of the surfactant to form micelles. The tail provides die hydrophobic portion of the micelle that allows the partitioning of the analyte molecule between the aqueous phase and the micelle's hydrophobic environment. The tail can vary in chain lengtii from 4 to 20 carbons, although in the preferred embodiment it is from 6 to 18 carbons, and may be either hnear or branched in structure. Changing the tail length also changes the critical micellar concentration (cmc). Different surfactant concentration allows one to modulate partitioning. The tail may also contain an ether or polyether portion in addition to its hydrophobic portion. Halogens may also be substituted for hydrogen on the carbon backbone. In particular, halogens such as chlorine, fluorine, bromine and iodine are preferred. Most preferred are chlorine and fluorine. In Example 21 it is demonstrated that the tail's lengtii may influence enantioselectivity. It is shown that the placement of a chiral center within the tail affects the alpha • values obtainable, as does die degree of branching. The second portion of die chiral surfactant is the hnkage between the hydrophobic tail portion of the molecule and die chiral center. Variation of this hnkage results in significant changes in enantioselectivity, possibly because the linker is physically next to the chiral selector. Four types of linkers have been studied and are presented herein. They are amides, carbamates, sulphonamides, and ureas. The amide linkers are demonstrated in Fig. 2(B) by the R-CO-NH-R' subunit. A carbamate linker is demonstrated in Fig. 3(A) by the R-OCO-NH-R' subunit. A sulphonamide linker is shown in Fig. 3(H) by die R-SO2-NH-R' subunit. The urea linker is shown

in Fig. 3(G) by die R-NH-CO-NH-R' subunit. One of the surprizing results of this invention is the fact tiiat the linkers have such a strong influence on the separation. As will be shown by the Examples, the substitution of one linker type for another can transform a marginal separation into one that works. Although not wishing to be bound by any theory of the invention, it is believed 5. that selection of the correct linker potentiates, or increases the chiral selectivity of the chiral selector. Other linkers not disclosed herein may also come within the scope of this invention if they potentiate the chiral selector.

The third portion of the molecule is the chiral center. The chiral center can be derived from any chiral compound. A chiral molecule is one that rotates the plane of polarized light. A 0 chiral molecule is defined as not being superimposable on its mirror image. (March, J., Advanced Organic Chemistry, Third ed., John Wiley & Sons, New York, 1985, p. 82) This invention demonstrates chiral compounds including amino acids, amino alcohols, and tartaric acid derivatives. .Amino acids and tiieir derivatives are particularly preferred chiral selectors because both enantiomers are available. Not only are die 20 essential amino acids included herein, but all 5 other amino acids tiiat contain at least one chiral center come within the scope of this invention. Examples are provided for die synthesis of at least 8 different amino acid-containing selectors, including phenylglycine, serine, valine, proline, aspartic acid, leucine, isoleucine, and tertiary- leucine. Amino alcohols are also demonstrated as chiral selectors. Representative of amino alcohols are valinol-, ephedrine-, and aminopropanediol-based chiral surfactants. Tartaric acid 0 derivatives contain two chiral centers in die center of the molecule, and two carboxyl groups at each end (See Figs. 3af-ai). Linkage of the tartaric acid to the surfactant is tiirough formation of a carboxamide group. Tartaric acid derivatives demonstrate die incorporation of two functionalities (head group and chiral selector) into one precursor molecule, reducing die number of synthetic steps required. Otiier bifunctional molecules can be made tiiat also incorporate two or more of the 5 four functions of the chiral surfactants of this invention.

The final portion of the molecule is the head group. This portion of the structure strongly influences the size, aggregation number, and solubility of the micelle. In a micelle, the head group is located on the periphery, oriented outwards towards the aqueous phase. The head group should contain an ionizable moiety so tiiat solubility with the aqueous phase is enhanced. Moietys that are 0 not ionzied at neutral pH may become charged when the pH is raised or lowered, thus enhancing solubility. The invention discloses tiiree types of head groups, carboxylates, sulfates, and alcohols. Carboxlyates (R-COOH) are the most numerous, and an example is demonstrated in Fig. 3(a). Sulfates (Sθ3θ ^~ M ⁺ ) are also useful as head groups, as demonstrated in Fig. 3(p). .Alcohols, -OH' are also used to enhance solubility, as shown in Fig. 3(i). Alcoholic head groups are examples of 5 compounds that are ionizable. Alcohols may become protonated at lower pH's, and become deprotonated at higher pH's, resulting in a net charge and enhanced solubility in aqueous media.

Other ionizable groups come within the scope of this invention, such as amines (NH), particularly quaternary amines (NR_4 ⁺ ) and ammonium salts NH3 ⁺ , sulfhydrals (SH), sulfonates (SO3 ^" ), amides (CONH), amidines, and guanidines.

Table 1 summarizes the different types of the four strucural units which have been prepared.

Table 1

Summary of Different Head Groups, Chiral Selectors, Linkers and Tails Which Have Been

Investigated

Head Groups Chiral Selectors Linkers Hydrophobic Tails alcohol tartrate urea polyedier-hydrocarbons sulfate amino acids carbamate cholesterohc carboxylate amino alcohols sulfonamide fluorohydrocarbon substituted ammonium salts amide C8-C18 hnear hydrocarbon, chiral branched tail

Figure 3 summarizes the variety of chiral surfactants that have been synthesized and tested for enantioselectivity using capillary electrophoresis. The surfactants consist of a group of carbamate linked surfactants, a group of urea linked surfactants, a sulfonamide linked surfactant, a polyetherhydrocarbon tail surfactant, a group of fluorohydrocarbon tail surfactants, a group of cholesteric tail surfactants, a group of chiral surfactants of varied tail length, a group of alcohol head group surfactants, a group of sulfate head group surfactants, a group of carboxylate head group surfactants, a group of amide linked surfactants, a group of tartrate-derived chiral selector- containing surfactants, a group of amino acid-derived chiral selector-containing surfactants, and a group of amino alcohol-derived chiral selector-containing surfactants. In some cases bodi enantiomers of a chiral surfactant have been used to show the reversal of enantiomer migration order. To date, a total of 68 surfactants have been syntiiesized and examined.

Examples Unless otherwise noted chemicals were obtained from Aldrich Chemical or Sigma. Example 1- Synthesis Of (S)-N-dodecoxycarbonylvaline (Fig. 3a)

To a three neck round bottom flask equipped with a thermometer, an addition funnel, and a mechanical stirring apparatus was added (S)-valine [Aldrich Chemical Company] (34.17 grams, 291 mmol), water (180 milhhters), and acetone (120 milliliters). The slurry was cooled to 0-5°C. Sodium hydroxide pellets (21.25 grams, 531 mmol) were added slowly to the slurry to maintain the temperature between 5-10°C. Dodecyl chloroformate (48.21 grams, 207 mmol), prepared by the

method of Richter and Tucker (Richter, R. and Tucker, B., Journal of Organic Chemistry, 1983, (48) 2625-2627), was added dropwise over 1 hour to the reaction mixture so that the temperature was maintained between 5-10°C during the addition. The reaction mixture was stirred at 0-5°C for 1 hour, warmed to ambient temperature, and stirred for 16 hours. The reaction mixture was a clear, slightly yellow solution. The solution was concentrated on a rotary evaporator, and the resulting aqueous phase was diluted with water (700 mL) and extracted with ethyl acetate (6x250 mL). The aqueous phase was cooled to 5-10°C and concentrated hydrochloric acid was added to reduce the pH to 1. The aqueous phase and oily precipitate was extracted witii ethyl acetate (4x 250 mL). The organic phase was evaporated to yield a white sohd (47.6 g, 73%) which was the desired product by IH-NMR and pure by HPLC (C18 reverse phase, methanol/water/acetic acid mobile phase).

Example 2 - Synthesis of Compounds of Figs. 3b-3f, Figs. 3aj-3ax

(R)-N-dodecoxycarbonylvaline (Fig. 3b), (S)-N-dodecoxycarbonyl-tert-leucine (Fig. 3c), (S)-N-tetradecoxycarbonylvahne (Fig. 3d), (S)-N-dodecoxycarbonylphenylglycine (Fig. 3e), and (S)-N-dodecylpolyoxyetiιylene(4)-oxycarbonylvahne (Fig. 3f) were also synthesized by tiiis procedure. The starting material for the synthesis of (S)-N-dodecylpolyoxyethylene(4)- oxycarbonylvaline was Brij 30 [Aldrich Chemical Company], a mixture of polyoxyethylene(4 to 12) lauryl and myristyl ethers. The product chiral surfactant was thus also a mixture.

The compounds of Figures 3aj-3ax were also prepared according to the synthetic procedure outiined above, with the exceptions that the chloroforamte used was based on the tail structure of the corresponding surfactant being syntiiesized.

Example 3. Synthesis Of die Compounds of Figs. 3g, 3ay

This urea linker compound was synthesized using the same procedure as Example 1, except that dodecyhsocyanate [Kodak] was used in place of dodecylchloroformate. The product was obtained in 73% yield and was pure by ^H-NMR and HPLC.

The compound of Figure 3 ay was also prepared according to die synthetic procedure outiined above, with the exception that decyhsocyanate was used in place of dodecyhsocyanate.

Example 4. Syntiiesis Of Compounds of Figs. 3h

This sufonamide was syntiiesized using the same procedure as in Example 1 except that dodecylsulfonyl chloride [Lancaster labs] was used in place of dodecylchloroformate. A precipitate consisting of the desired product and dodecanesulfonic acid formed during the reaction. The desired product was isolated by preparative HPLC (Cl 8 reverse phase, methanol/water/acetic acid, 50% yield) and was pure by 1 H-NMR and HPLC.

Example 5 - Synthesis Of Compunds of Figs. 3s-3y

This amide-linker, Fig. 3w, was synthesized using die same procedure as in Example 1 except tiiat dodecanoyl chloride was used in place of dodecylchloroformate. The product was isolated via methylene chloride extraction in 97% yield. The product was used for subsequent CE experiments as is (containing 3% lauric acid); or pure product was isolated by preparative HPLC

(C18 reverse phase, methanol/water/acetic acid).

(S)-N-dodecanoylphenylglycine (Fig. 3s), (S)-N-dodecanoylserine (Fig. 3t), (S)-N- dodecanoylprohne (Fig. 3u), (S)-N-dodecanoylaspartic acid (Fig. 3v), (S)-N-octanoylvaline (Fig.

3x), and (S)-N-hexadecanoylvaline (Fig. 3y) were syntiiesized similarly.

Examples 6 - Synthesis of the Compounds of Fig. 3ab-ae, and Figs. 3az- 3bc

To a three neck round bottom flask equipped witii a thermometer, an addition funnel, and a mechanical stirring apparatus was added (S)-valinol (25.9 grams, 251 mmol) and dichloromethane

(180 mL). The solution was cooled to 0-5°C under nitrogen. Dodecanoyl chloride (27.3 grams, 125 mmol) in dichlorometiiane (120 mL) was added dropwise over 1.5 hour to the reaction mixture while maintaining the temperature between 5-10°C. The reaction mixture was stirred at 0-5°C for

1 hour, warmed to ambient temperature, and stirred for 17 hours. A white precipitate was observed. To remove ammonium salts, the reaction mixture was extracted witii water (1 x 250 mL), aqueous 0.1 molar hydrochloric acid (1 x 250 mL), and water (1 x 250 mL). The organic solution was concentrated on die rotary evaporator, and die resulting white sohd was dried in vacuo overnight to afford die desired product (35.0 g, 98% yield). The product, (S)-N-dodecanoylvahnol

(Fig. 3ab), was pure by HPLC and 1H NMR.

(S)-N-octanoylvahnol (Fig. 3ac), (lS,2R)-N-dodecanoylephedrine (Fig. 3ad), and

(lS,2S)-N-amino-l-phenyl-l,3-propanediol dodecanamide (Fig. 3ae) were syntiiesized similarly. The compounds of Figs. 3az- 3bc were also prepared according to the synthetic procedure outiined above.

Example 7. Synthesis of the Compounds of Figs. 3i-k

This carbamate-linker, (S)-N-dodecoxycarbonylvahnol, was syntiiesized using die same procedure as the amide, (S)-N-dodecanoylvalinol, (Ex. 6) except tiiat dodecylchloroformate was used in place of the acid chloride.

(S)-N-Cholesteroxycarbonylvahnol (Fig. 3j) was syntiiesized similarly from valinol and cholesteryl chloroformate.

(S)-N-dodecylaminocarbonylvahnol (Fig. 3k) was synthesized similarly from valinol and dodecyhsocyanate.

Example 8 - Synthesis Of the Compounds of Figs. 31-o, Figs. 3bd-bg

To a three neck round bottom flask equipped with a thermometer, an addition funnel, and a mechanical stirring apparatus was added (S)-N-dodecanoylvalinol (5.00 grams, 17.5 mmol) and dichloromethane (120 mL). The solution was cooled to 0-5°C under nitrogen. Chlorosulfonic acid (2.04 grams, 17.5 mmol) in dichloromethane (30 mL) was added dropwise over 0.5 hour to the reaction mixture while maintaining the temperature between 5-10°C. The reaction mixture was stirred at 0-5°C for 2 hours. The solution was concentrated on die rotary evaporator, and the remaining white sohd was dried in vacuo. To the solid was added metiianol (100 mL), R.O. water (10 mL), and sodium hydroxide pellets (0.700 grams, 17.5 mmol). When dissolution of the pellets was complete, the solution was concentrated on die rotary evaporator, and the remaining white sohd was dried in vacuo for 17 hours at 25°C. The identity of the product, (S)-2-[(l- oxododecyl)amino]-3-methyl-l-sulfooxy-butane (Fig. 3m), (6.64 g, 98% yield) was confirmed by IH-NMR.

(S)-2-[(l-oxododecoxy)amino]-3-metiιyl-l-sulfooxybutane (Fig. 31), (S)-2-[(l- oxododecylamino)amino]-3-metiιyl-l-sulfooxybutane (Fig. 3n), and (S)-[Oxocholesteroxylamino]- 3-methyl-l-sulfoxybutane (Fig. 3o) were synthesized by similar procedures.

The compounds of Figs. 3bd- 3bg were also prepared according to the synthetic procedure outiined above.

Example 9 - Synthesis Of Compounds of Figs. 3z ((S)-N-pentadecafluorooctanoylvaline) via (S)- N-pentadecafluorooctanoylvaline metiiyl ester.

To a three neck round bottom flask equipped with a thermometer, an addition funnel, and a mechanical stirring apparatus was added (S)-valine metiiyl ester hydrochloride (5.81 g, 34.7 mmol), dichloromethane (350 mL), and triethylamine (8.53 g, 84.3 mmol). The solution was cooled to 0-5°C under nitrogen. Pentadecafluorooctanoyl chloride (15.0 grams, 34.7 mmol) in dichloromethane (50 mL) was added dropwise over 0.5 hour while maintaining die temperature between 5-10°C. The reaction mixture was stirred at 0-5°C for 1 hour, warmed to ambient temperature, and stirred for 2 hours. A white precipitate was observed. The reaction mixture was extracted with water (1 x 250 mL), aqueous 0.1 molar hydrochloric acid (1 x 250 mL), and water ( 1 x 250 mL). The solution was concentrated on a rotary evaporator, and the resulting white sohd was dried in vacuo overnight to afford die desired product (18.2 g, 99% yield). The product was shown to be pure by HPLC and 13C-NMR.

To a three neck round bottom flask equipped with a thermometer, a stopper, and a mechanical stirring apparatus was added (S)-N-pentadecafluorooctanoylvahne methyl ester (2.00 g, 3.8 mmol), tetrahydrofuran (100 mL), and water (22 mL). The solution was cooled to 0-5°C. Potassium hydroxide pellets (0.70 grams, 12.5 mmol) were slowly added. After 12 hours, the

reaction vessel was stoppered and placed in the refrigerator for another 12 hours. The solution was concentrated on a rotary evaporator, and the remaining solution was diluted witii water (150 mL)and cooled to 5-10°C. The solution was acidified with concentrated hydrochloric acid to pH 1. The temperature was maintained between 5-15°C during die addition. A white precipitate was formed. The aqueous phase and precipitate were extracted witii ethyl acetate (3 x 150 mL). The organic solution was concentrated on a rotary evaporator to afford a white sohd which was dried in vacuo overnight to afford the desired product (2.02 g, 97% yield). 13C NMR spectroscopic and HPLC analysis showed die product to be pure.

Example 10 - Synthesis Of Compounds of Fig. 3ag ((R,R)-N-dodecyltartaric acid monoamide via (R,R)-N-dodecyl-0,0-diacetyltartaric acid monoamide, Fig. 3ai, (R,R)-N-decyltartaric acid monoamide, and Fig. 3bf, (R,R)-N-Octyltartaric acid monoamide

To a tiiree neck round bottom flask equipped with a thermometer, an addition funnel, and a mechanical stirring apparatus was added 1-dodecylamine (17.15 g, 92.5 mmol), tetrahydrofuran (350 mL), and triethylamine (9.36 grams, 92.5 mmol). The solution was cooled to 0-5°C under nitrogen. (R,R)-0,0-diacetyltartaric anhydride (20.0 g, 92.5 mmol) in tetrahydrofuran (150 mL) was added dropwise over 1 hour to the reaction mixture while maintaining the temperature between 5-10°C. The reaction mixture was stirred at 0-5°C for 1 hour, warmed to ambient temperature, and stirred for 4 hours. The solution was concentrated on a rotary evaporator, and die resulting brown, viscous oil was dried in vacuo overnight to afford crude product (46.1 grams) in 99% yield. To a three neck round bottom flask equipped witii a thermometer, a stopper, and a mechanical stirring apparatus was added (R,R)-N-dodecyl-0,0-diacetyltartaric acid monoamide (triethylamine form; 6.18 g, 12.3 mmol), methanol (150 mL), and water (15 mL). Sodium hydroxide (1.62 grams, 41.0 mmol) was added to the reaction mixture. After 0.5 hours, a white precipitate formed. The reaction mixture was heated to 55°C, and stirred for 18 hours. The sohd was filtered off and dried in vacuo to afford product (3.96 g, 95% yield) which was shown to be pure by 1H NMR.

(R,R)-N-decyltartaric acid monoamide (Fig. 3ai) (sodium form) and Fig. 3bf, (R,R)-N- Octyltartaric acid monoamide, were syntiiesized via a similar two step procedure.

Example 11 - Synthesis of the Compounds of Figs. 3bh-bn

To a 500 millilrter three neck round bottom flask equipped with a thermometer, an addition funnel, and a mechanical stir apparatus was added N-t-BOC-(S)-proline (Sigma Chemical Company, 13.69 g, 63.6 mmol), tri hylamine (6.44 g, 63.6 mmol), and dichloromethane (250 mL). The solution was cooled to 0-5 °C under nitrogen. Isobutyl

chloroformate (-Aldrich Chemical Company, 8.69 g, 63.6 mmol) in dichloromethane (50 mL) was slowly added dropwise over 0.5 hour to the reaction mixture while maintaining the temperature between 0-5 °C. After the addition was complete, the reaction mixture was stirred at 0-5°C for 15 min. 1-Dodecylamine (11.79 g, 63.6 mmol) in dichloromethane (50 mL) was then slowly added dropwise over 0.5 hour to the reaction mixture while maintaining the temperature between 0-5°C. The reaction mixture was stirred at 0-5°C for 2 hours, warmed up to ambient temperature, and then stirred for 16 hours. The reaction mixture was extracted with aqueous hydrochloric acid (0.1 N; 2 x 100 mL), aqueous sodium hydroxide (0. I N; 2 x 100 mL), and R.O. water (2 x 100 mL). Solvent was evaporated off on the rotary evaporator, and the remaining white solid was dried in vacuo overnight to afford crude product (24.02 g) in 99% yield. ^l H NMR spectroscopic and HPLC analysis (C 18 reverse phase, methanol/water/acetic acid mobile phase) showed that the desired product was obtained and was pure.

N-Dodecyl-(S)-prolinamide hydrochloride was obtained by reacting the crude product (24.0 g, 62.7 mmol) described above with concentrated aqueous hydrochloric acid (12N, 480 mmol) in methanol (160 mL) at 25°C for 16 hours. Solvent was evaporated off on the rotary evaporator, and the remaining white sohd was dried in vacuo overnight to afford crude product (19.68 g) in 98% yield. *H NMR spectroscopic and HPLC analysis (Cl 8 reverse phase, methanol/water/acetic acid mobile phase) showed that the desired product was obtained and was pure.

This same procedure was used for the synthesis of N-octyl-(S)-valinamide hydrochloride (Fig. 3bi), N-decyl-(S)-valinamide hydrochloride (Fig. 3bh), N-tetradecyl- (S)-valinamide hydrochloride (Fig. 3bj), N-dodecyl-(S)-alamnamide hydrochloride (Fig. 3bk), N-dodecyl-(S)-leucinamide hydrochloride (Fig. 3bl), and N-dodecyl-(S)- isoleucinamide hydrochloride (Fig. 3bm).

Examples 12-17. Separation of Enantiomers

When a surfactant is added to an aqueous environment above its critical micelle concentration (cmc), individual surfactant molecules aggregate to form a structure called a micelle. A model of this structure is seen in Figure 4 (the surfactant sodium dodecyl sulfate is shown as an example). The long hydrophobic tails of die surfactant become buried together within the structure, while the polar head group is hydrated witii the water of the' electrolyte. Once the sample is injected into the capillary, it has the opportunity to interact with the chiral micelles formed in the electrolyte.

If the compound or a portion of it is hydrophobic, it will partition within the hydrophobic environment of the micelle.

The micelle has a characteristic charge/mass ratio which confers electrophoretic mobihty to the micelle. Mobihties for the surfactants of this invention vary from about 1 to 5 xlO-4 cm2/V- sec. As the result of its electrophoretic mobihty the micelle will migrate through the capillary with a characteristic migration time, t _m c which depends on die capillary length and field strength. When an analyte is partitioned within the micelle, it will also display die apparent mobihty of the micelle. If a molecule partitions totally within the aqueous phase, then it will have its own characteristic electrophoretic mobihty defined by its charge/mass ratio. In Figure 5, a vector diagram of micelle (-4x10-4 cm2 Vs) and electroosmotic (+1x10-4 cm2/Vs) mobilities is shown, as well as the apparent mobihty of a neutral analyte (-1.5x10-4 cm2/Vs) which spends 50% of the time in the micelle and 50% of die time in bulk solution. Once partitioned within the micelle, the sample analyte has the opportunity to interact with the chiral center. If the sample molecules partition within the micelle only a portion of the time and die sample enantiomers differentially interact with the chiral center, the two enantiomers will have shghtiy different apparent mobihties, and can then be separated. Optimum levels of partitioning, or k, that result in maximum resolution are determined by the efficiency and selectivity of the separation system as shown in equation 2.

Experimental Apparatus and Conditions for Examples 12-17 Capillary electrophoretic separations were performed witii a Waters Quanta® 4000 CE unit. Separations were performed in a 50 μm i.d. x 60 cm uncoated or polyethylene glycol coated (J&W Scientific) fused silica capillary, 52.5 cm injection to detection. Apphed voltage was 12 kV, generating currents ranging from 20-60 μAmps. Injection was achieved by raising the inlet end of the capillary immersed in the sample solution to a height of 10 cm above the outiet end for 2-5 seconds. On-column UV detection was performed at 214 nm. Buffers were prepared from phosphate or phosphate/borate stock, and adjusted to die desired pH after solubilization of the surfactant. Data collection was achieved witii Waters ExpertEase™ or Millenium™ software (Waters Corporation, Milford, MA).

Example 12-Separation of Atenolol and Effect of Surfactant Concentration on Partitioning

The abihty to modulate partitioning is an important element of the present invention. This was demonstrated in the separation of atenolol using a C12-carbamate-linked valine surfactant (Fig 3a) seen in Figure 6. At a surfactant concentration of 25 mM (Fig. 6A), k= 0.8, alpha=1.04 and resolution Rs= 1.0. As the surfactant concentration was increased to 100 mM (Fig. 6B), k increased to 2.2, and the resolution to 2.5, with alpha remaining constant. In this manner, partitioning was optimized by changing the surfactant concentration. In order to optimize the

system for samples with large k's, a much lower surfactant concentration can be employed. As seen in Figure 7, propranolol was almost totally partitioned within the micelle at 25 mM (Fig. 7A), with no enantiomeric resolution. However, by lowering the concentration to 5 mM (Fig. 7B) and decreasing k, resolution emerges. However, as the detergent concentration is lowered to the range of the cmc, plates rapidly decrease.

Example 13-Effect of a Change in Hydrophobicity

Another way to modulate partitioning is to change the hydrophobic character of the surfactant. Figure 8 shows a chiral separation of the hydrophobic racemate oxprenolol using a vahne modified Brij-30 detergent (Fig. 3f). Because the Brij surfactant is more hydrophihc than the previously used hydrocarbons, hydrophobic analytes which partition too strongly into the micelles of the previous example show the desired reduction in partitioning and improved separations. Other chain compositions such as polyethers and glycols are also possible.

Example 14-Effect of Halogenated Tail Hydrocarbons

It is also possible to modulate die partitioning through halogenation of the hydrocarbon chain of the surfactant. Figure 9 shows a separation of bupivacaine employing a vahne modified fluorinated hydrocarbon (Fig. 3z). The corresponding hydrocarbon surfactant does not even form micelles under these conditions.

Example 15-Effect of Changing Hydrophobicity of the Electrolyte

Partitioning can also be altered by adding organic solvent to the MEKC buffer. This is shown in Figure 10, where propranolol is separated witii 25 mM (S)-N-Dodecoxycarbonylvahne (Fig. 3 a) and 30% acetonitrile. Recall that in Figure 7A, propranolol was completely partitioned into the micelle at 25 mM in the absence of organic solvent.

Example 16-Effect of Changing Chiral Selector on Selectivity

The selectivity of the chiral separation can be modulated by changing the chiral selector. A variety of amino acids may be employed. Figure 11 shows the use of several amino acid-modified detergents at 25 mM concentration in the separation of some PTH-amino acids. Figure 11 A shows the separation of PTH-alanine with a valine-based surfactant (Fig. 3q) and a phenyglycine-based surfactant (Fig. 3s), witii the phenylglycine surfactant showing resolution. Figure 1 IB shows the separation of PTH-norvaline with proline- (Fig. 3u), phenylgycine- and valine-based surfactants. In tiiis case, the phenylglycine surfactant shows the best resolution, although all three show some resolution. Figure 11 C and 1 ID show separation of PTH-tryptophan on serine- (Fig. 3t), vahne-, proline- and phenylglycine-based surfactants, with the valine surfactant showing the best

resolution. By using a screen of sample analytes it is clear that some amino acid selectors work better than others. These data are summarized in Table 2.

Table 2

Alpha Values for Several Enantiomeric Pairs Obtained with 6 Different Amino Acid-Modified

Detergents

Compound Valine Phenyl¬ Aspartic Serine Proline tertiary- glycine Acid Leucine benzoin 1.057 1.039 1.000 1.000 1.000 1.000

PTH-alanine 1.000 1.067 1.000 - 1.000 1.000

PTH-norleucine 1.122 1.213 1.000 1.096 1.000 1.000

PTΗ-norvaline 1.119 1.181 1.000 1.000 1.040 1.000

PTH-serine 1.000 - 1.000 - ' - 1.000

PTH-tryptophan 1.266 1.196 1.000 1.108 1.036 1.000

PTH-valine 1.125 1.180 1.000 1.116 1.035 1.000

Non-amino acid chiral selectors may also be employed. .Amino alcohols, organic acids including tartaric acids, cyciodextrins, sugar monomers, ohgosaccharides, and other organic chiral molecules may be employed for this purpose. Examples using vahnol (Fig. 3k) (12A), tartaric acid (Fig. 3ag) (12B) and (lS,2S)-N-2-aιnino-l-phenyl-l,3-propranedioldodecanamide (Fig. 3ae) (12C) are seen in Figure 12.

Example 17-Effect of Linker on Selectivity

The immediate chemical environment of the chiral center may also lead to important changes in selectivity. An unexpectedly effective way to modulate the environment of the chiral center is by changing the chemical group that links the chiral center to the hydrophobic tail of the surfactant. As seen in Figure 13, the use of sulfonamide (Fig. 3h) (13A), urea (Fig. 3g) (13B), and carbamate (Fig. 3a) (13C) hnkers can change die selectivity of a particular separation dramatically. Table 3 shows a list of 14 compounds that have been screened under a single set of conditions using surfactants containing tiiese three hnkers.

Table 3

Alpha and Resolution Differences of 14 Test Racemates on Urea, Sulfonamide and Carbamate Linked Surfactants

Analyte Urea Carbamate Sulfonamid Urea Carbamate Sulfonamid α α α Rs Rs Rs atenolol 1.03 1.04 1.02 0.30 0.80 0.50 bupivacaine 1.14 1.05 1.04 2.16 1.16 0.80 ephedrine 1.09 1.10 1.08 1.56 1.81 0.89 homatotropine 1.10 1.03 1.00 1.20 0 0 ketamine 1.04 1.01 1.00 1.03 0.24 0 metoprolol 1.03 1.06 1.00 0.20 1.00 0

N-methyl- 1.04 1.32 1.29 0.25 2.50 2.00 pseudoephedrine nadolol (1) 1.02 1.03 1.04 0.30 0.55 1.15 nadolol (2) 1.00 1.00 1.05 0 0 0.86 norephedrine 1.06 1.10 1.06 1.16 1.74 1.05 norphenylephrine 1.05 1.09 1.06 0.90 1.79 0.73 octopamine 1.02 1.05 1.02 0.60 0.54 0.23 pindolol 1.03 1.06 1.04 0.25 1.06 0.73 terbutaline 1.06 1.01 1.03 2.12 0.40 0.69

Clearly, optimum selectivity and resolution are dependent upon the specific interaction of the sample analyte and the surfactant. Although differences in alpha that are shown in Table 3 appear small, a difference of 0.01-0.03 can dramatically improve performance. Figure 14 shows an example of a chiral separation with alphas of 1.01 ( 14 A, carbamate linker) and 1.04 ( 14B, urea linker). Given similar plates and partitioning, this represents a four-fold increase in resolution. The improvement in the quality of the separation is dramatic. The surfactants can also be used in mixtures that perform more separations than a given single surfactant. Table 4 shows data for a termolecular mixture of these surfactants which is effective in separating 13 of 14 compounds.

Table 4

Separation Data for 14 Racemates Separated with 15mM each of Sulfonamide-, Urea- and

Carbamate-Linked Surfactants

Analyte k Rs α measured atenolol 2.18 1.20 1.03 bupivacaine 10.9 2.22 1.06 ephedrine 5.18 3.06 1.07 homatotropine 4.74 2.07 1.04 ketamine 3.28 1.05 1.02 metoprolol 8.42 1.53 1.03

N-methyl-pseudoephedrine 2.80 6.17 1.19 nadolol (1) 4.49 1.60 1.04 nadolol (2) 4.72 1.12 1.05 norephedrine 4.39 2.67 1.06 norphenyl-ephrine 1.69 2.87 1.08 octopamine 0.97 1.16 1.03 pindolol 7.09 1.73 1.04 terbutaline 2.88 0 1.00

There is no one universal chiral center, linker, tail or head group that solves all problems. Flexibility to change the selectivity and partitioning is critical to the overall success in chiral separations. Unlike the circumstance of HPLC or GC where changing columns involves operator intervention, many CE instruments allow automated buffer selection. Thus, a set of surfactants as described herein can be automatically screened to identify the best separation.

Example 18-Effect of pH on Chiral Separations

As mentioned above, in the normal operation of this invention, the analyte must partition within the micelle. In some cases this interaction can be modulated by changing the pH of the aqueous environment. For instance, negatively charged analytes typically do not partition into negatively charged micelles. Examples of two acids are shown in Fig. 15. However, if die pH is lowered so the carboxyhc acid functionalities of the analyte are protonated, then the now-neutral analyte can more easily partition within the micelle. However, previously demonstrated embodiments of this invention also contain a carboxylate functionality that is essential to the solubihty of the surfactant. When these surfactants are rendered neutral, they are no longer soluble. Therefore, a charged head group that will retain its charge throughout the usable pH range would allow the separation of acids. However, the addition of a different head group, such as sulfate

dramatically changes this property. In one embodiment a sulfate group is substituted for a carboxylate functionality in a C12 amide-linked vahnol detergent (Fig. 3m). Separations may now be conducted down to pH 2.0 using this surfactant. Figure 16 shows chiral separations of the organic acids in Figure 15 that were not possible at elevated pH of 6 or above (16A is proglumide, 16B CBZ-tryptophan). Other head groups such as sulfonic acids, sulfonates, alcohols, and diols are also possible.

Another type of molecule that was separated using the present invention are 6- quinolylaminocarbonyl-tagged amino containing compounds, as shown in Figure 17. A typical separation is shown in Figure 18.

Example 19-Effect of Electroosmotic Flow

One of the side effects of electrophoresis in a fused sihca capillary tube can be a bulk fluid flow called electroosmotic flow. This fluid flow moves all components of the separation at the same velocity through the capillary, without contributing to the resolution of the chiral separation. This is demonstrated in Figure 19. AtpH 4.0 (19A), the mobihty of the osmotic flow was 1.4x 10'^cm^ /Vs, while the micelle mobihty is -4.4x 10 ^' ^cm^ /Vs. When the pH was increased to 6.0 ( 19B) the micelle mobihty remained the same, while the osmotic mobihty increases 2.28x. This enhancement of the electroosmotic flow had no impact on the observed resolution, but resulted in an overall increase in the analysis time of several minutes. In a different embodiment of this invention, the electroosmotic flow was greatly reduced with the use of a fused sihca capillary with a modified wall chemistry. An embodiment of this is shown in Figure 20, using 25 mM (S)-N-dodecoxycarbonylvahne at pH 7 (coated capillary is 20A, μos=0.7 x 10"^ cm^ Vs, uncoated capillary 20B, μos=5.7 x 10-4 cm^/Vs). In this case, the presence of the osmotic flow reduces the analysis time, but does not improve resolution.

Example 20-Detection of Ephedrine Enantiomers in Urine

MEKC is also useful for performing separations in physiological samples. Fig. 21 is a electropherogram showing the separation of ephedrine enantiomers in untreated urine. Fig. 21(A) is 100 ug/ml racemic ephedrine standard solution. Baseline separation occurs between 17 and 18 minutes. Fig. 21(B) is a sample of 100 ug/ml ephedrine-spiked urine run under the same conditions, with separation occurring at approximately the same time. Fig. 21 (C) is a urine blank. Note the absence of any interfering peaks in the area between

17 and 18 minutes. The abihty to separate both chiral and achiral compounds in the urine samples illustrates the broad applicability of this method.

Conditions: capillary 50 urn i.d. by 60 cm length; +15 kV applied voltage; detection at 214 nm, 0.1 second time constant; data aquisition at 10 points/sec; injection over 5 sees. Buffer was 25mM phosphate/borate, pH 8.8, with 50 mM N-dodecoxycarbonylvaline. Sample preparation consisted of filtration only.

Example 21 -Tail potentiating enantioselectivity

To demonstrate that the tail potentiates enantioselectivity, alpha values obtained for ten analytes were compared using four novel chiral surfactants which have the same linker (carbamate), chiral selector (valine) and head (carboxylate), but different tails. The four novel chiral surfactants were (S)-N-dodecyloxyethylene-(4)oxycarbonylvahne (Fig. 3f, Brij-30 tail), (S)-N-dodecoxycarbonylvaline (Fig. 3a, linear hydrocarbon tail), (S)-N-(2R)- octoxycarbonylvaline (Fig. 3av, chiral (R), branched hydrocarbon tail), and (S)-N-(2S)- octoxycarbonylvahne (Fig. 3au, chiral (S), branched hydrocarbon tail). All separations were performed at pH 8.8 with either 25 mM (straight hydrocarbon tail), 50 mM (Brij-30 tail) or 100 mM (R and S branched hydrocarbon tails) surfactant.

Alphas for the test compounds on each tail are given in Table 5 below. The linear hydrocarbon tail generally had the highest alpha, except in the cases of nadolol, octopamine and terbutaline. For terbutaline, ( S )-N-(2R)-octoxy carbonylvaline (R tail) had the highest alpha, for nadolol (1) (S)-N-(2S)-octoxy carbonylvaline (S tail) had the highest alpha, for nadolol (2) (S)-N-dodecyloxyethylene-(4)oxycarbcnylvaline had the highest alpha, and for oct amine (S)-N-(2S)-ortoxycarbcmylvaline (S tail) had the highest alpha. Note that for all compounds the alphas were different on at least one of the tails.

Table 5

Analvte Brii-30 Tail Hvdrocarbon Tail α Chiral R Tail α Chiral S Tail α α bupivacaine 1.02 1.05 1.01 1.02 ephedrine 1.03 1.10 1.07 1.10 homatropine 1.01 1.03 1.03 1.02 ketamine 1.00 1.01 1.00 1.00 nadolol (1) 1.03 1.03 1.03 1.04 nadolol (2) 1.04 1.00 1.00 1.00 norphenylephrine 1.05 1.09 1.06 1.09 octopamine 1.03 1.05 1.04 1.07 pindolol 1.04 1.06 1.05 1.05 terbutaline 1.03 1.01 1.05 1.00

Other tail types also influence enantioselectivity. Table 6 compares enantioselectivity data obtained with (S)-N-perfluorooctanoyl-(L)-valine (perfluoro tail, amide linker, valine chiral selector, carboxylate head) to (S)-N-dodecanoylvaline (hydrocarbon tail, amide linker, valine chiral selector, carboxylate head). In all cases, the two tails showed different enantioselectivity values. Conditions were 25 mM surfactant at pH 8.8.

Table 6

.Analyte Perfluoro Tail α Hydrocarbon Tail α bupivacaine 1.07 1.06 ephedrine 1.04 1.05 homatropine 1.00 1.02 metoprolol 1.00 1.01 nadolol (1) 1.03 1.00 nadolol (2) 1.02 1.00 terbutaline 1.03 1.00

Example 22 - Head Potentiating Enantioselectivity

To demonstrate that the hydrophihc head potentiates enantioselectivity, alpha values obtained for eleven compounds on six novel chiral surfactants were compared. These six surfactants all have C12 tails and valine-based chiral selectors. Sulfate and carboxylate head groups were compared for three linkers (amide, carbamate and urea). The six novel surfactants are (S)-N-dodecanoylvaline (Fig. 3q, amide-valine-carboxylate), (S)- N-dodecoxycarbonylvaline (Fig. 3a, carbamate-valine-carboxylate), (S)-N- dodecylaminocarbonylvaline (Fig. 3g, urea-valine-carboxylate), (S)-2-[(l-oxododecyl)- amino]-3-methyl-l -sulfooxybutane (Fig. 3m, ai de-valinol-sulfate), (S)-2-[(l- oxododecoxy)amino]-3-methyl-l -sulfooxybutane (Fig. 31, carbamate- valinol-sulfate) and (S)-2-[(l-oxododecylamώo)amino]-3-memyl-l-sulfooxybutane (Fig. 3n, urea-valinol- sulfate). Conditions were 25 mM surfactant atpH 8.8.

Table 7 below gives the comparison data of sulfate vs. carboxylate for each linker. In most cases the alpha was different due to the two head groups. In general, the carboxylate head group was superior.

Table 7 Amide Carbamate Urea

Analyte Sulfate Carbox. α Sulfate α Carbox. α Sulfate α Carbox. α atenolol 1.01 1.00 1.05 1.04 1.00 1.03 bupivacaine 1.10 1.06 1.08 1.05 1.09 1.14 homatropine 1.00 1.02 1.01 1.03 1.06 1.10 ketamine 1.02 1.05 1.02 1.01 1.02 1.04 metoprolol 1.00 1.01 1.05 1.06 1.00 1.03 nadolol (1) 1.03 1.00 1.02 1.03 1.03 1.02 nadolol (2) 1.03 1.00 1.14 1.00 1.00 1.00 norphenyl-ephrine 1.02 1.09 1.03 1.09 1.02 1.05 octopamine 1.00 1.00 1.00 1.05 1.00 1.02 pindolol 1.00 1.02 1.04 1.06 1.00 1.03 terbutaline 1.00 1.00 1.02 1.01 1.04 1.06

Changing the charge on the head group can also dramatically influence enantioselectivity, and hence resolution. Figure 22A shows the separation of N-benzoyl- DL-alanine with (S)-2-[(l-oxododecyl)ammo]-4-memyl-l-sulfooxypentane ( Fig. 3bd, C12-amide-leucinol-sulfate), while Figure 22B shows the separation of the same

compound on N-dodecyl-(S)-leucmamide hydrochloride (Fig. 3bl, C12-amide-leucine- ammonium salt). Superior enantioselectivity and resolution is obtained with the positively charged ammonium head group. Conditions were 25 mM surfactant at pH 3.0.

Example 23 - Chiral Selector Potentiating Enantioselectivity

Comparison data for several novel chiral surfactants which have carbamate linkers, C12 tails and carboxylate heads, but different amino acid chiral selectors, is shown below in Table 8. Ten chiral bases were examined with (S)-N-dodecoxycarbc«ιylalanine (Fig. 3aj), (S)-N-decoxycarbonylasparagine (Fig. 3ar ), (2S,3S)-N-dodecoxycarbonylisoleucine (Fig. 3al), (S)-N-decoxycarbonylleucine (Fig. 3ak), (S)-N-dodecoxycarbonyltertleucine (Fig. 3c), (2S,3S)-N-dodecoxycarbonylthreonine (Fig. 3as) and (S)-N- dodecoxycarbonylvaline (Fig. 3 a). Enantioselectivity values obtained for the ten compounds on each amino acid derivative are given below. Conditions were 25 mM surfactant at pH 8.8. Overall, the leucine derivative showed superior enantioselectivity. Furthermore, it showed enantioselectivity for the chiral base aπφhetamine which contains only one hydrogen bonding site, see Figure 23. Other chiral surfactants which have shown the abihty to separate amphetamine are (S)-N-dodecoxycarbcnylcyclohexyldanine (Fig. 3ao, Figure 24A), and (R,R)-N-decyltartaric acid monoamide, sodium salt (Fig. 3ag, Fig. 24B). Table 8

.Analyte Ala Asp lie Leu t-Leu Thre Val amphetamine 1.00 1.00 1.00 1.02 1.00 1.00 1.00 bupivacaine 1.03 1.00 1.13 1.03 1.06 1.09 1.05 ephedrine 1.08 1.04 1.11 1. •14 1.09 1.07 1.10 homatropine 1.02 1.03 1.02 1.03 1.00 1.00 1.03 ketamine 1.01 1.00 1.01 1.02 1.02 1.00 1.01 metoprolol 1.04 1.03 1.06 1.08 1.04 1.05 1.06 norephedrine 1.07 1.04 1.10 1.11 1.09 1.07 1.10 noφhenylephrme 1.10 1.04 1.08 1.10 1.06 1.07 1.09 pindolol 1.05 1.04 1.06 1.08 1.04 1.06 1.06 terbutaline 1.01 1.00 1.00 1.00 1.00 1.01 1.01

Example 24 - Hydrophihc Head Groups Influence Partitioning

As previously demonstrated sulfate head groups have an advantage over carboxylate head groups in terms of their abihty to be used at acidic pH. At acidic pH, separation of hydrophihc acids can be obtained since the charge on the acids is inirώiiized and partitioning is higher than at neutral pH where they are fully anionic. The reason for the low partiticming at neutral pH is due to charge repulsion of the anionic analyte by the anionic micelle. A positively charged head group also leads to improved partitioning and resolution of anionic compounds, since charge attraction will increase partitioning. For example, Figure 25 shows the separation of the anionic compound carboxybenzoyl-DL- alanine using 25 mM N-dodecyl-(S)-prolinamide hydrochloride (Fig. 3bn) at pH 3.0.

Example 25 - pH Influencing Partitioning and Enantioselectivity

In this study, 25 mM (S)-N-dodecoxycarbonylvaline (Fig. 3a) was used to separate twelve basic analytes and one neutral one (benzoin) at pHs 7.0 and 8.8. Table 9 below gives free solution mobihty data (μ), capacity factor values (k) and alpha values for all thirteen compounds at the two pHs. For all the analytes except benzoin (neutral at both pHs), free solution mobihty was higher at pH 7.0. These results were expected, since all the basic analytes contain a ino groups with pK _a s in the pH 7-9 range. At lower pH, the compounds had more positive charge, leading to the increased mobihty. For all the analytes except benzoin, capacity factor values were higher at pH 7.0, which is attributable to the increased ionic attraction between the anionic micelles and catiomc analyte. Finally, alpha values were the same or higher for all the basic compounds at pH 7.0 vs. pH 8.8. Note especially the large increase in alpha for bupivacaine, ketamine and metoprolol. None of the bases showed lower enantioselectivity at pH 7.0.

This data clearly indicates the importance of charge in deteπnining partitioning and enantioselectivity for cationic analytes with anionic micelles. Similar trends should be expected with anionic analytes and cationic micelles.

TABLE 9 μ α

ANALYTE pH 7.0 pH 8.8 pH 7.0 pH 8.8 pH 7.0 DH 8.8 atenolol 1.44 1.29 1.94 1.56 1.05 1.04 benzoin 0 0 2.35 2.41 1.04 1.04 bupivacaine 1.54 0.41 40.5 7.62 1.26 1.05 ephedrine 2.16 1.79 6.60 3.77 1.14 1.10 homatropine 1.79 1.66 4.10 3.35 1.03 1.03 ketamine 1.58 0.13 12.5 2.20 1.06 1.01 metoprolol 1.58 1.30 26.5 7.44 1.19 1.06

N-methyl- 2.45 1.44 4.05 1.96 1.38 1.32 pseudoephedrine nor-ephedrine 2.18 1.42 6.10 3.44 1.12 1.10 noφhenylephrin 1.99 0.94 2.02 1.01 1.09 1.09 ortopamine 1.97 1.03 1.09 0.64 1.05 1.05 pindolol 1.84 1.44 9.50 6.70 1.09 1.06 terbutaline 1.82 1.60 3.32 2.40 1.02 1.01

Example 26 - Tail Length Enabling Solubihty

In chiral surfactants αmtaining carboxylate head groups, it was found that there is a minimum pH below which the surfactant is insoluble. This minimum pH value is a function of the length of the hydrocarbon tail. For instance, for surfactants containing a carbamate linker, valine chiral selector and carboxylate head group, the derivative with a C 14 tail, (S)- N-tetrade∞xycarbonylvaline, is insoluble below pH 7.5, the derivative with a C12 tail, (S)- N-dodecoxycarbonylvaline, is insoluble below pH 6.5, the derivative with a CIO tail, (S)- N-decoxycarbonylvaline, is insoluble below pH 5.5, and the derivative with a C8 tail, (S)- N-octoxycarbonylvaline, is insoluble below pH 4.5. It is important to have a wide range of pH solubihty, since enantioselectivity and partitioning is influenced by pH for ionizable compounds (see above). The tradeoff for the wider pH range of solubihty is a higher critical micelle concentration (cmc).

An example of how tail length can improve resolution through better pH solubihty is shown in the separation of nicotine enantiomers, Figures 26A and 26B. The separation shown in Figure 26 A was obtained with 100 mM (S)-dodecylaminocarbonylvahne (Fig. 3g, C 12 tail) at pH 8.0. complete resolution and peak tailing were evident. The tailing was due to the fact that the surfactant was insoluble at pH 7.8, and at the analysis pH of 8.0 surfactant was binding to the wall. Resolution was incomplete because the enantioselectivity was too low. The separation in Figure 26B was obtained with 100 mM (S)-decylammocarbonylvaline (Fig. 3ay, CIO tail) atpH 7.5. Baseline resolution and symmetrical peaks were obtained, since at this pH the C 10 derivative was well above its minimum pH of solubihty (pH 6.7), and since higher enantioselectivity was obtained.

Example 27 - Separation of Aspartame Stereoisomers

Aspartame, or Nutrasweet™ (GD Searle, Chicago, IL), is an artificial sweetener used in products ranging from diet soft drinks to pharmaceuticals. Aspartame is a dipeptide of aspartic acid and phenylalanine, with the C-terminus protected as the methyl ester. Since the dipeptide has two chiral centers, a total of four stereoisomers are possible; LL, DD, LD, DL. The LL isomer is marketed as the sweetener. However, it is important to monitor for the other stereoisomers as well, both in the raw material as well as in the finished product. Figure 27 shows separation of all four stereoisomers using 25 mM (S)- 2-[(l- oxododecyl)-amino]-3-methyl- 1 -sulfooxybutane (Fig. 3m) at pH 3.5. The four stereoisomers were baseline resolved within 19 minutes. The later peaks at 22 and 24 minutes were probably due to breakdown products of the main isomers.

Example 28 - Effect of Linker on UV Background In order to show the influence of the linker on the resulting background UV absorbance of the chiral MECC buffer, the following experiment was conducted:

25 mM solutions of (S)-N-dodecanoylvaline (amide linker), (S)-N- dodecylammocarbonylvaline (urea linker), and (S)-N-dodecoxycarbonylvaline (carbamate linker) were individually prepared in 25 mM Na ₂ HPO /25 mM Na ₂ B ₄ O ₇ buffer. Using a 50μm i.d. capillary and 214 nm UV detection, deionized water was drawn into the capillary with vacuum and the absorbance set to 0.000 absorbance units. The UV absorbance was then measured when blank buffer, 25 mM (S)-N-dodecanoylvaline in buffer, 25 mM (S)- N-dodecylaminocarbonylvaline in buffer, and 25mM (S)-N-dodecoxycarbonylvahne in

buffer were drawn into the capillary with vacuum. The absorbance of each solution is given in Table 10 below.

Table 10 Solution Absorbance (Absorbance units)

Blank Buffer 0.000

25 mM amide linker in buffer 0.037

25 mM urea hnker in buffer 0.026

25 mM carbamate linker in buffer 0.007

As seen from the table, the amide hnker led to the highest absorbance, 1.4 times the urea's absorbance and 5.3 times the carbamate' s absorbance. High background absorbance is a large disadvantage in CE, since it will increase the noise level and decrease the linear range of detection. Note that a surfactant with two amide groups would show roughly 2 time the absorbance of one with only one amide group (assuming no other parts of the surfactant are UV active). This data should clearly show the disadvantage of amide linkers in chiral surfactants when employing UV absorbance detection, as well as highhght the advantage of the carbamate linker.

Although the foregoing invention has been described by way of illustration and example for puφoses of clarity and understanding, it will be apparent that certain changes and modifications may be practiced within the scope of the invention, as limited only by the scope of the appended claims. For instance, the chiral selector may be one or more chiral carbons, arranged proximal to one another, or separated by some distance. The Tartaric Acid derivatives presented herein are but one example of a multiple chiral selector arrangement. The specific chiral selector is not limited, but rather may be selected from any class of chiral molecules imaginable, and still come within the scope of this invention. Similarly, the tail portion of the molecule, although primarily present to enhance hydrophobic interaction (partitioning) with the chiral analyte, may also contain a chiral carbon, as demonstrated herein. Chiral centers may be situated anywhere within the tail so that they may interact with the chiral center of the analyte. Head groups may be of any composition, so long as they fulfill the primary function of solubilizing the molecule in the solvent that the micelles are supported in, and potentiate enantioselectivity. Any hnker molecules that potentiate the chiral selectivity of the chiral selector come within the scope of this invention.