Title:
CHROMOGENIC SUBSTRATE WITH A PH INDICATOR DYE
Document Type and Number:
WIPO Patent Application WO2004097367
Kind Code:
A3
Abstract:
A composition for an enzyme-linked assay is described which comprises a chromogenic substrate that produces a detectable color with a maximum absorbance at one wavelength in reactions of the assay which contain the enzyme and a pH indicator dye that produces a detectable color with a maximum absorbance at a second wavelength when the pH of the reactions of the assay is changed to terminate the reactions. The chromogenic substrate enables colorimetric detection of the bindable substance in positive reactions of the assay and the pH indicator dye enables negative reactions of the assay to be colorimetrically distinguished from false negative 3,3',5,5'-tetramethylbenzidine (TMB) and the pH indicator dye is preferably selected from the group consisting of cresol red and m-cresol purple.
Inventors:
MAYER BRENT A
Application Number:
PCT/US2004/010356
Publication Date:
March 24, 2005
Filing Date:
April 02, 2004
Export Citation:
Assignee:
NEOGEN CORP (US)
International Classes:
C12Q1/28; G01N33/58; (IPC1-7): G01N33/53; C12Q1/28
Domestic Patent References:
| WO1999001768A1 | 1999-01-14 |
Foreign References:
| US6221624B1 | 2001-04-24 | |||
| US4444879A | 1984-04-24 | |||
| US5215885A | 1993-06-01 | |||
| US4946776A | 1990-08-07 |
Other References:
SHELEF ET AL.: "Novel selective and non-selective optical detection of microorganisms", LETTERS IN APPLIED MICROBIOLOGY, vol. 25, 1997, pages 202 - 206, XP002904880
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