Title:
CITRUS TRISTEZA VIRUS BASED VECTORS FOR FOREIGN GENE/S EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2013/044023
Kind Code:
A3
Abstract:
Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode heterologous polypeptide s. The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.
Inventors:
DAWSON WILLIAM O (US)
FOLIMONOVA SVETLANA Y (US)
EL-MOHTAR CHOAA (US)
FOLIMONOVA SVETLANA Y (US)
EL-MOHTAR CHOAA (US)
Application Number:
PCT/US2012/056565
Publication Date:
June 27, 2013
Filing Date:
September 21, 2012
Export Citation:
Assignee:
UNIV FLORIDA (US)
International Classes:
A01H5/00; C12N15/11; C12N15/83
Foreign References:
| US20100333230A1 | 2010-12-30 | |||
| US20100017911A1 | 2010-01-21 |
Other References:
FOLIMONOVA, SVETLANA Y. ET AL.: "Infection with strains of Citrus tristeza virus does not exclude superinfection by other strains of the virus", JOURNAL OF VIROLOGY., vol. 84, no. 3, February 2010 (2010-02-01), pages 1314 - 1325, XP055072179
FAGOAGA, CARMEN ET AL.: "Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza virus Confers Resistance to the Virus in Transgenic Mexican Lime", PLANT MOLECULAR BIOLOGY, vol. 60, no. 2, January 2006 (2006-01-01), pages 153 - 165, XP019262827
EHRENGRUBER, MAKRUS U ET AL.: "Semliki forest virus vectors with mutations in the nonstructural protein 2 gene permit extended superinfection of neuronal and non-neuronal cells", JOURNAL OF NEUROVIROLOGY, vol. 13, no. 4, 2007, pages 353 - 363
FAGOAGA, CARMEN ET AL.: "Post-Transcriptional Gene Silencing of the p23 Silencing Suppressor of Citrus tristeza virus Confers Resistance to the Virus in Transgenic Mexican Lime", PLANT MOLECULAR BIOLOGY, vol. 60, no. 2, January 2006 (2006-01-01), pages 153 - 165, XP019262827
EHRENGRUBER, MAKRUS U ET AL.: "Semliki forest virus vectors with mutations in the nonstructural protein 2 gene permit extended superinfection of neuronal and non-neuronal cells", JOURNAL OF NEUROVIROLOGY, vol. 13, no. 4, 2007, pages 353 - 363
Attorney, Agent or Firm:
VAN DYKE, Timothy, H. (390 N. Orange Ave. Suite 250, Orlando FL, US)
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