Title:
CLASS OF ARSENITE INHIBITORY FACTOR REPORTER GENE PLASMIDS, CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/061381
Kind Code:
A1
Abstract:
Provided are a class of arsenite inhibitory factor reporter gene plasmids, a construction method therefor and an application thereof, the plasmids being pLHPars9 and pLLPars9, and the nucleotide sequences thereof being as shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The plasmids are sensitive to arsenite, the detection range being 0.04～50 μM, and the plasmids may be used as a biosensor for use in the detection of arsenite. In addition, arsenite inhibitory factor reporter gene plasmid pLHPars9 may further be used for detecting antimonite.
Inventors:
LI XIANQIANG (CN)
FANG YUN (CN)
JIANG XIN (US)
XU MEIYING (CN)
GUO JUN (CN)
FANG YUN (CN)
JIANG XIN (US)
XU MEIYING (CN)
GUO JUN (CN)
Application Number:
PCT/CN2017/104721
Publication Date:
April 04, 2019
Filing Date:
September 30, 2017
Export Citation:
Assignee:
GUANGDONG INST MICROBIOLOGY GUANGDONG DETECTION CT MICROBIOLOGY (CN)
SIGNOSIS INC (US)
SIGNOSIS INC (US)
International Classes:
C12N15/64; C12N1/21; C12N15/70; C12Q1/02; C12Q1/66; C12R1/19
Domestic Patent References:
| WO2003102223A1 | 2003-12-11 | |||
| WO2016133830A1 | 2016-08-25 | |||
| WO2014012089A1 | 2014-01-16 |
Foreign References:
| CN104894042A | 2015-09-09 | |||
| US20130337464A1 | 2013-12-19 |
Other References:
ROBERTO, F.F. ET AL.: "Evaluation of a GFP Reporter Gene Construct for Environmental Arsenic Detection", TALANTA, vol. 58, no. 1, 16 August 2002 (2002-08-16), pages 181 - 188, XP055587813, ISSN: 0039-9140
DATABASE Nucleotide 9 March 2016 (2016-03-09), BUTCHER, B.G. ET AL.: "Acidithiobacillus ferridurans strain ATCC 33020 arsenate reductase (arsC), ArsR-like protein, arsenical membrane pump (arsB), ArsH-like protein, signal recognition particle protein-like protein, 30S ribosomal protein S16-like protein, and 16S ribosomal RNA pro...", XP055587831, retrieved from NCBI Database accession no. AF173880.1
DATABASE Nucleotide 9 March 2016 (2016-03-09), BUTCHER, B.G. ET AL.: "Acidithiobacillus ferridurans strain ATCC 33020 arsenate reductase (arsC), ArsR-like protein, arsenical membrane pump (arsB), ArsH-like protein, signal recognition particle protein-like protein, 30S ribosomal protein S16-like protein, and 16S ribosomal RNA pro...", XP055587831, retrieved from NCBI Database accession no. AF173880.1
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (CN)
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