Title:
CLDN18.2-TARGETING ANTIBODY, BISPECIFIC ANTIBODY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/016348
Kind Code:
A1
Abstract:
The present invention discloses CLDN18.2-targeting antibodies, bispecific antibodies and use thereof. The CLDN18.2-targeting antibody is a single-domain heavy-chain antibody that has high affinity for tumor cells endogenously expressing CLDN18.2 and can induce high endocytic activity. The bispecific antibody can target CLDN18.2 and CD3 and retains the binding effect of an Fc to an FcRn; meanwhile, a mutant Fc is preferred so as to reduce the binding to an FcgR and thus the activation of non-specific T cells caused by the cross-linking of an FcgR. The CD3-terminus activity is optimized so that the release of common cytokines in CRS, such as IL6 and TNFα can be reduced.
Inventors:
JHENG MING-JIN (CN)
WANG YONGQIANG (CN)
ZHANG YUN (CN)
ZHAO CHUCHU (CN)
YANG YUNXING (CN)
CHEN FEI (CN)
QIN BEIBEI (CN)
WU YUETAO (CN)
DING YI (CN)
WANG YONGQIANG (CN)
ZHANG YUN (CN)
ZHAO CHUCHU (CN)
YANG YUNXING (CN)
CHEN FEI (CN)
QIN BEIBEI (CN)
WU YUETAO (CN)
DING YI (CN)
Application Number:
PCT/CN2022/110314
Publication Date:
February 16, 2023
Filing Date:
August 04, 2022
Export Citation:
Assignee:
HARBOUR BIOMED SHANGHAI CO LTD (CN)
International Classes:
C07K16/28; A61P35/00
Domestic Patent References:
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| WO2020238730A1 | 2020-12-03 | |||
| WO2021011885A1 | 2021-01-21 | |||
| WO2014075788A1 | 2014-05-22 | |||
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| WO2014146672A1 | 2014-09-25 | |||
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FRONT PHARMACOL, vol. 9, 13 September 2018 (2018-09-13), pages 404
"Genbank", Database accession no. NP_001002026
MOL CELL BIOL, vol. 21, no. 21, November 2001 (2001-11-01), pages 7380 - 90
EUR J CANCER, vol. 100, September 2018 (2018-09-01), pages 17 - 26
BIRD ET AL., SCIENCE, vol. 242, 1988, pages 423 - 426
HUSTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 5879 - 5883
DORONINA, BIOCONJUGATE CHEM, vol. 17, 2006, pages 114 - 124
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH (U.S.
JMOL BIOL, vol. 273, 1997, pages 927 - 48
J. BIOL. CHEM, vol. 243, 1968, pages 3558
YU ET AL., PNAS, vol. 99, 2002, pages 7968 - 7973
Attorney, Agent or Firm:
CHINA PATENT AGENT (H.K.) LTD. (CN)
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