Thereof

1

Background ofthe Invention

Field ofthe Invention

5 The present invention relates to a substantially pure thermostable DNA polymerase. Specifically, the DNA polymerase of the present invention is a Thermotoga DNA polymerase and more specifically a Thermotoga neapolitana (Tne) DNA polymerase or Thermotoga maritima (Tma) DNA polymerase. Preferably, the polymerase has a molecular weight of about 100 kilodaltons. The

10 present invention also relates to the cloning and expression of the Thermotoga

DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to hosts which express said genes. The DNA polymerase ofthe present invention may be used in DNA sequencing, amplification reactions, and cDNA synthesis.

This invention also relates to mutants of the Thermotoga DNA

15 polymerase, including Tne and Tma DNA polymerase. Specifically, the DNA polymerases ofthe present invention have mutations which substantially reduce 3'-5' exonuclease activity; mutations resulting in the ability ofthe mutant DNA polymerase to incorporate dideoxynucleotides into a DNA molecule about as efficiently as deoxynucleotides; and mutations which substantially reduce 5'-3'

20 exonuclease activity. The Thermotoga (e.g., Tne and Tma) mutant DNA polymerase of this invention can have one or more of these properties. These DNA polymerase mutants may also be used in DNA sequencing, amplification reactions, and cDNA synthesis.

The present invention is also directed to novel mutants of other DNA

*.

25 polymerases which have substantially reduced 5 '-3 ' exonuclease activity.

Background Information

DNA polymerases synthesize the formation of DNA molecules which are complementary to a DNA template. Upon hybridization of a primer to the single- stranded DNA template, polymerases synthesize DNA in the 5' to 3' direction, successively adding nucleotides to the 3 '-hydroxyl group ofthe growing strand.

Thus, in the presence of deoxyribonucleoside triphosphates (dNTPs) and a primer, a new DNA molecule, complementary to the single stranded DNA template, can be synthesized.

A number of DNA polymerases have been isolated from mesophilic microorganisms such as E coli. A number of these mesophilic DNA polymerases have also been cloned. Lin et al. cloned and expressed T4 DNA polymerase in E. coli (Proc. Natl. Acad. Sci. USA 54:7000-7004 (1987)). Tabor et al. (U.S. Patent No. 4,795.699) describes a cloned T7 DNA polymerase, while Minkley et al. (J. Biol. Chem. 259: 10386- 10392 (1984)) and Chatterjee (U.S. Patent No. 5,047,342) described E. coli DNA polymerase I and the cloning of T5

DNA polymerase, respectively.

Although DNA polymerases from thermophiles are known, relatively little investigation has been done to isolate and even clone these enzymes. Chien et al., J. Bacteriol. 727:1550-1557 (1976) describe a purification scheme for obtaining a polymerase from Thermus aquaticus (Taq). The resulting protein had a molecular weight of about 63,000 daltons by gel filtration analysis and 68,000 daltons by sucrose gradient centrifugation. Kaledin et al, Biokhymiya 45:644-51 (1980) disclosed a purification procedure for isolating DNA polymerase from T. aquaticus YT1 strain. The purified enzyme was reported to be a 62,000 dalton monomeric protein. Gelfand et al. (U.S. Patent No. 4,889,818) cloned a gene encoding a thermostable DNA polymerase from Thermus aquaticus. The molecular weight of this protein was found to be about 86,000 to 90,000 daltons.

Simpson et al. purified and partially characterized a thermostable DNA polymerase from a Thermotoga species (Biochem. CeU. Biol. 5(5:1292-1296

(1990)). The purified DNA polymerase isolated by Simpson et al. exhibited a molecular weight of 85,000 daltons as determined by SDS-polyacrylamide gel electrophoresis and size-exclusion chromatography. The enzyme exhibited half- lives of 3 minutes at 95 °C and 60 minutes at 50 °C in the absence of substrate and its pH optimum was in the range of pH 7.5 to 8.0. Triton X- 100 appeared to enhance the thermostability of this enzyme. The strain used to obtain the thermostable DNA polymerase described by Simpson et al. was Thermotoga species strain FJSS3-B.1 (Hussar et aL, FEMS Microbiology Letters 37:121-127 (1986)). Others have cloned and sequenced a thermostable DNA polymerase from Thermotoga maritima (U.S. Patent 5,374,553, which is expressly incorporated herein by reference).

Other DNA polymerases have been isolated from thermophilic bacteria including Bacillus steraothermophilus (Stenesh et al. , Biochim. Biophys. Acta 272:156-166 (1972); and Kaboev et al, J. Bacteriol. 145:21-26 (1981)) and several archaebacterial species (Rossi et al, System. Appl. Microbiol. 7:337-341

(1986); Klimczak et al, Biochemistry 25:4850-4855 (1986); and Elie et al, Eur. J. Biochem. 75:619-626 (1989)). The most extensively purified archaebacterial DNA polymerase had a reported half-life of 15 minutes at 87°C (Elie et al. (1989), supra). Innis et al, In PCR Protocol: A Guide To Methods and Amplification. Academic Press, Inc., San Diego (1990) noted that there are several extreme thermophilic eubacteria and archaebacteria that are capable of growth at very high temperatures (Bergquist et al , Biotech. Genet. Eng. Rev. 5:199-244 (1987); and Kelly et al, Biotechnol. Prog. 4Λ7-62 (1988)) and suggested that these organisms may contain very thermostable DNA polymerases. In many of the known polymerases, the 5 '-3' exonuclease activity is present in the N-terminal region of the polymerase. (Ollis, et al., Nature 313:762-766 (1985); Freemont et al., Proteins 7:66-73 (1986); Joyce, Cur. Opin. Struct. Biol. 7:123-129 (1991).) There are some amino acids, the mutation of which are thought to impair the 5 '-3' exonuclease activity of E. coli DNA polymerase I. (Gutman & Minton, Nucl. Acids Res. 21 :4406-4407 (1993).) These

amino acids include Tyr ⁷⁷ , Gly ¹⁰³ , Gly ¹⁸⁴ , and Gly ¹⁹² in E. coli DNA polymerase

I. It is known that the 5 '-exonuclease domain is dispensable. The best known example is the BClenow fragment of E. coli polymerase I. The Klenow fragment is a natural proteolytic fragment devoid of 5 '-exonuclease activity (Joyce et. al, J. Biol. Chem. 257:1958-64 (1990).) Polymerases lacking this activity are useful for DNA sequencing.

Most DNA polymerases also contain a 3 '-5' exonuclease activity. This exonuclease activity provides a proofreading ability to the DNA polymerase. A

T5 DNA polymerase that lacks 3 '-5' exonuclease activity is disclosed in U.S. Patent No. 5,270,179. Polymerases lacking this activity are particularly useful for DNA sequencing.

The polymerase active site, including the dNTP binding domain is usually present at the carboxyl terminal region of the polymerase (Ollis et al, Nature 313:762-766 (1985); Freemont et al.. Proteins 7:66-73 (1986)). It has been shown that Phe ⁷⁶² of E. coli polymerase I is one ofthe amino acids that directly interacts with the nucleotides (Joyce & Steitz, Ann. Rev. Biochem. 63:777-822 (1994); Astatke, J. Biol. Chem. 270:1945-54 (1995)). Converting this amino acid to a Tyr results in a mutant DNA polymerase that does not discriminate against dideoxynucleotides. See copending U.S. Application No. 08/525,087, of Deb K. Chatterjee. filed September 8, 1995, entitled "Mutant DNA Polymerases and the

Use Thereof," which is expressly incorporated herein by reference.

Thus, there exists a need in the art to develop more thermostable DNA polymerases. There also exists a need in the art to obtain wild type or mutant DNA polymerases that are devoid of exonuclease activities and are non- discriminating against dideoxynucleotides.

Summary ofthe Invention

The present invention satisfies these needs in the art by providing additional DNA polymerases useful in molecular biology. Specifically, this invention includes a thermostable DNA polymerase. Preferably, the polymerase has a molecular weight of about 100 kilodaltons. Specifically, the DNA polymerase ofthe invention is isolated from Thermotoga, and more specifically, the DNA polymerase is obtained from Thermotoga neapolitana (Tne) and Thermotoga maritima (Tma). The Thermotoga species preferred for isolating the DNA polymerase of the present invention was isolated from an African continental solfataric spring (Windberger et al, Arch. Microbiol. 151. 506-512,

(1989)).

The Thermotoga DNA polymerases ofthe present invention are extremely thermostable, showing more than 50% of activity after being heated for 60 minutes at 90°C with or without detergent. Thus, the DNA polymerases ofthe present invention is more thermostable than Taq DNA polymerase.

The present invention is also directed to cloning a gene encoding a Thermotoga DNA polymerase enzyme. DNA molecules containing the Thermotoga DNA polymerase genes, according to the present invention, can be transformed and expressed in a host cell to produce the DNA polymerase. Any number of hosts may be used to express the Thermotoga DNA polymerase gene ofthe present invention; including prokaryotic and eukaryotic cells. Preferably, prokaryotic cells are used to express the DNA polymerase ofthe invention. The preferred prokaryotic host according to the present invention is E. coli.

The present invention also relates mutant thermostable DNA polymerases ofthe Poll type and DNA coding therefor, wherein there is amino acid change in the O-helix which renders the polymerase nondiscriminatory against ddNTPs in sequencing reactions. The O-helix is defined as RXXXKXXXFXXXYX, wherein X is any amino acid.

The present invention also relates to Thermotoga DNA polymerase mutants that lack exonuclease activity and/or which are nondiscriminatory against ddNTPs in sequencing reactions.

The present invention is also directed generally to DNA polymerases that have mutations that result in substantially reduced or missing 5 '-3 ' exonuclease activity.

In particular, the invention relates to a Thermotoga DNA polymerase mutant which is modified at least one way selected from the group consisting of

(a) to reduce or eliminate the 3 '-5' exonuclease activity of the polymerase;

(b) to reduce or eliminate the 5 '-3' exonuclease activity of the polymerase; and

(c) to reduce or eliminate discriminatory behavior against a dideoxynucleotide. The invention also relates to a method of producing a DNA polymerase, said method comprising:

(a) culturing the host cell ofthe invention;

(b) expressing said gene; and

(c) isolating said DNA polymerase from said host cell. The invention also relates to a method of synthesizing a double-stranded

DNA molecule comprising:

(a) hybridizing a primer to a first DNA molecule; and

(b) incubating said DNA molecule of step (a) in the presence of one or more deoxy- or dideoxyribonucleoside triphosphates and the DNA polymerase of the invention, under conditions sufficient to synthesize a second DNA molecule complementary to all or a portion of said first DNA molecule. Such deoxy- and dideoxyribonucleoside triphosphates include dATP, dCTP, dGTP, dTTP, dITP, 7-deaza-dGTP, 7-deaza-dATP, dUTP, ddATP, ddCTP, ddGTP, ddlTP, ddTTP, [α-S]dATP, [α-S]dTTP, [α-S]dGTP, and [α-S]dCTP.

The invention also relates to a method of sequencing a DNA molecule, comprising:

(a) hybridizing a primer to a first DNA molecule;

(b) contacting said DNA molecule of step (a) with deoxyribonucleoside triphosphates, the DNA polymerase ofthe invention, and a terminator nucleotide;

(c) incubating the mixture of step (b) under conditions sufficient to synthesize a random population of DNA molecules complementary to said first DNA molecule, wherein said synthesized DNA molecules are shorter in length than said first DNA molecule and wherein said synthesized DNA molecules comprise a terminator nucleotide at their 3 ' termini; and

(d) separating said synthesized DNA molecules by size so that at least a part ofthe nucleotide sequence of said first DNA molecule can be determined. Such terminator nucleotides include ddTTP, ddATP, ddGTP, ddlTP or ddCTP. The invention also relates to a method for amplifying a double stranded

DNA molecule, comprising:

(a) providing a first and second primer, wherein said first primer is complementary to a sequence at or near the 3 '-termini ofthe first strand of said DNA molecule and said second primer is complementary to a sequence at or near the 3 '-termini of the second strand of said DNA molecule;

(b) hybridizing said first primer to said first strand and said second primer to said second strand in the presence of the DNA polymerase of the invention, under conditions such that a third DNA molecule complementary to said first strand and a fourth DNA molecule complementary to said second strand are synthesized;

(c) denaturing said first and third strand, and said second and fourth strands; and

(d) repeating steps (a) to (c) one or more times.

The invention also relates to a kit for sequencing a DNA molecule, comprising:

(a) a first container means comprising the DNA polymerase of the invention; (b) a second container means comprising one or more dideoxyribonucleoside triphosphates; and

(c) a third container means comprising one or more deoxyribonucleoside triphosphates .

The invention also relates to a kit for amplifying a DNA molecule, comprising:

(a) a first container means comprising the DNA polymerase of the invention; and

(b) a second container means comprising one or more deoxyribonucleoside triphosphates. The present invention also relates to a mutant DNA polymerase having substantially reduced or eliminated 5 '-3' exonuclease activity, wherein at least one of the amino acids coπesponding to Asp ⁸ , Glu" ² , Asp" ⁴ , Asp" ⁵ , Asp ¹³⁷ , Asp ¹³⁹ , Gly ¹⁰² , Gly ¹⁸⁷ , or Gly ¹⁹⁵ of Tne DNA polymerase has been mutated.

The present invention also relates to a method of producing a mutant DNA polymerase having substantially reduced or eliminated 5 '-3' exonuclease activity, wherein at least one ofthe amino acids coπesponding to Asp ⁸ , Glu ¹¹² , Asp" ⁴ . Asp" ⁵ , Asp ¹³⁷ , Asp ¹³⁹ , Gly ¹⁰² , Gly ¹⁸⁷ , or Gly ¹⁹⁵ of Tne DNA polymerase has been mutated, comprising:

(a) culturing the host cell ofthe invention; (b) expressing the mutant DNA polymerase; and

(c) isolating said mutant DNA polymerase.

Brief Description ofthe Figures

FIG. 1 demonstrates the heat stability of Tne DNA polymerase at 90 °C over time. Partially purified DNA polymerase from the crude extract of Thermotoga neapolitana cells was used in the assay. FIG. 2 shows the time-dependent DNA polymerase activity of Tne DNA polymerase isolated from an E. coli host containing the cloned Tne DNA polymerase gene.

FIG. 3 compares the ability of various DNA polymerases to incorporate radioactive dATP and [αS]dATP. Tne DNA polymerase is more effective at incorporating [αSJdATP than was Taq DNA polymerase.

FIG.4 shows the restriction map ofthe approximate DNA fragment which contains the Tne DNA polymerase gene in pSport 1 and pUC19. This figure also shows the region containing the O-helix homologous sequences.

FIGS. 5A and 5B shows the nucleotide and deduced amino acid sequences, in all 3 reading frames, for the carboxyl terminal portion, including the O-helix region, ofthe Thermotoga neapolitana polymerase gene.

FIG. 6A schematically depicts the construction of plasmids pUC-Tne (3'~5') and pUC-Tne FY.

FIG. 6B schematically depicts the construction of plasmids pTrc Tne35 and pTrcTne F Y.

FIG. 7 schematically depicts the construction of plasmid pTrcTne35 FY.

FIG. 8 schematically depicts the construction of plasmid pTTQTne5 FY and pTTQTne535FY.

FIG. 9 depicts a gel containing two sequencing reaction sets showing the efficient ³⁵ S incorporation by 7>je DNA polymerase of Example 12. Alkali- denatured pUC 19 DNA was sequenced with Tne DNA polymerase in set A. M 13 mpl9(+) DNA was sequenced in set B.

FIG. 10 depicts a gel containing three sequencing reaction sets showing that the mutant Tne DNA polymerase of Example 12 generates clear sequence

from plasmids containing cDNAs with poly(dA) tails. Alkali-denatured plasmid DNAs containing cDNA inserts were sequenced using either Tne DNA polymerase (sets A and B), or Sequenase Ver 2.0 (set C).

FIG. 11 depicts a gel containing three sequencing reaction sets that compare the mutant Tne DNA polymerase of Example 12 (set A), Sequenase™

(set B) and Taq DNA polymerase (set C) generated sequences from a plasmid containing poly(dC).

FIG. 12 depicts a gel containing three sequencing reaction sets showing that the mutant Tne DNA polymerase of Example 12 (set A) produces ³⁵ S-labeled sequence 3-fold stronger than Thermo Sequenase™ (set B) and without the uneven band intensities obtained with Taq DNA polymerase (set C).

FIG. 13 depicts a gel containing four sequencing reaction sets demonstrating that the mutant Tne DNA polymerase of Example 12 produces high quality sequences of in vitro amplified DNA (set A, E. coli β poll (~450bp); set B, E. coli πsE (-350 bp); set C, ori from pSClOl (-1.5 kb); and set D, an exon from human HSINF gene (-750 bp).

FIGS. 14A and 14B depict gels containing three and four sequencing reaction sets, respectively, showing that the mutant Tne DNA polymerase of Example 12 provides superior sequence from double-stranded DNA clones containing poly(dA) or poly(dC) stretches. Fig. 14A, supercoiled plasmid DNAs containing inserts with homopolymers were cycle sequenced using the mutant Tne DNA polymerase (set A, RPA1; set B, elf (cap binding protein); and set C, a poly(dC)-tailed 5' RACE-derived insert). Fig. 14B, supercoiled plasmid DNAs containing inserts with homopolymers were cycled sequenced using Taq DNA polymerase (set D), or SequiTherm™ (sets E-G) (set D, RPA; set E, RPA; set F, a poly(dC)-tailed 5' RACE-derived insert; and set G, elf).

FIG. 15 depicts a gel containing two sequencing reaction sets showing cycle sequencing using the mutant Tne DNA polymerase of Example 12 and ³² P end-labeled primer.

FIGS. 16A-16C and 16D-16F depict two sets of chromatograms showing comparison of the mutant Tne DNA polymerase of Example 12 (16A-16C) to AmpliTaq FS™ (16D-16F) in Fluorescent Dye Primer Sequencing.

FIGS. 17A-17C and 17D-17F depict chromatograms showing a comparison of the mutant Tne DNA polymerase of Example 12 (17A) to

AmpliTaq FS™ (17B) in Fluorescent Dye Terminator Sequencing.

Detailed Description ofthe Preferred Embodiments

Definitions

In the description that follows, a number of terms used in recombinant DNA technology are extensively utilized. In order to provide a clearer and consistent understanding ofthe specification and claims, including the scope to be given such terms, the following definitions are provided.

Cloning vector. A plasmid, cosmid or phage DNA or other DNA molecule which is able to replicate autonomously in a host cell, and which is characterized by one or a small number of restriction endonuclease recognition sites at which such DNA sequences may be cut in a determinable fashion without loss of an essential biological function ofthe vector, and into which DNA may be spliced in order to bring about its replication and cloning. The cloning vector may further contain a marker suitable for use in the identification of cells transformed with the cloning vector. Markers, for example, are tetracycline resistance or ampicillin resistance.

Expression vector. A vector similar to a cloning vector but which is capable of enhancing the expression of a gene which has been cloned into it, after transformation into a host. The cloned gene is usually placed under the control of (i.e., operably linked to) certain control sequences such as promoter sequences.

Recombinant host. Any prokaryotic or eukaryotic or microorganism which contains the desired cloned genes in an expression vector, cloning vector

or any DNA molecule. The term "recombinant host" is also meant to include those host cells which have been genetically engineered to contain the desired gene on the host chromosome or genome.

Host. Any prokaryotic or eukaryotic microorganism that is the recipient of a replicable expression vector, cloning vector or any DNA molecule. The

DNA molecule may contain, but is not limited to, a structural gene, a promoter and/or an origin of replication.

Promoter. A DNA sequence generally described as the 5' region of a gene, located proximal to the start codon. At the promoter region, transcription of an adjacent gene(s) is initiated.

Gene. A DNA sequence that contains information necessary for expression of a polypeptide or protein. It includes the promoter and the structural gene as well as other sequences involved in expression ofthe protein.

Structural gene. A DNA sequence that is transcribed into messenger RNA that is then translated into a sequence of amino acids characteristic of a specific polypeptide.

Operably linked. As used herein means that the promoter is positioned to control the initiation of expression ofthe polypeptide encoded by the structural gene. Expression. Expression is the process by which a gene produces a polypeptide. It includes transcription ofthe gene into messenger RNA (mRNA) and the translation of such mRNA into polypeptide(s).

Substantially Pure. As used herein "substantially pure" means that the desired purified protein is essentially free from contaminating cellular contaminants which are associated with the desired protein in nature.

Contaminating cellular components may include, but are not limited to, phosphatases, exonucleases, endonucleases or undesirable DNA polymerase enzymes.

Primer. As used herein "primer" refers to a single-stranded oligonucleotide that is extended by covalent bonding of nucleotide monomers during amplification or polymerization of a DNA molecule.

Template. The term "template" as used herein refers to a double-stranded or single-stranded DNA molecule which is to be amplified, synthesized or sequenced. In the case of a double-stranded DNA molecule, denaturation of its strands to form a first and a second strand is performed before these molecules may be amplified, synthesized or sequenced. A primer, complementary to a portion of a DNA template is hybridized under appropriate conditions and the DNA polymerase of the invention may then synthesize a DNA molecule complementary to said template or a portion thereof. The newly synthesized DNA molecule, according to the invention, may be equal or shorter in length than the original DNA template. Mismatch incorporation during the synthesis or extension ofthe newly synthesized DNA molecule may result in one or a number of mismatched base pairs. Thus, the synthesized DNA molecule need not be exactly complementary to the DNA template.

Incorporating. The term "incorporating" as used herein means becoming a part of a DNA molecule or primer.

Amplification. As used herein "amplification" refers to any in vitro method for increasing the number of copies of a nucleotide sequence with the use of a DNA polymerase. Nucleic acid amplification results in the incorporation of nucleotides into a DNA molecule or primer thereby forming a new DNA molecule complementary to a DNA template. The formed DNA molecule and its template can be used as templates to synthesize additional DNA molecules. As used herein, one amplification reaction may consist of many rounds of DNA replication. DNA amplification reactions include, for example, polymerase chain reactions (PCR). One PCR reaction may consist of 30 to 100 "cycles" of denaturation and synthesis of a DNA molecule.

Oligonucleotide. "Oligonucleotide" refers to a synthetic or natural molecule comprising a covalently linked sequence of nucleotides which are

joined by a phosphodiester bond between the 3' position of the pentose of one nucleotide and the 5' position ofthe pentose ofthe adjacent nucleotide.

Nucleotide. As used herein "nucleotide" refers to a base-sugar-phosphate combination. Nucleotides are monomeric units of a nucleic acid sequence (DNA and RNA). The term nucleotide includes deoxyribonucleoside triphosphates such as dATP, dCTP, dITP, dUTP, dGTP, dTTP, or derivatives thereof. Such derivatives include, for example, [αS]dATP, 7-deaza-dGTP and 7-deaza-dATP. The term nucleotide as used herein also refers to dideoxyribonucleoside triphosphates (ddNTPs) and their derivatives. Illustrated examples of dideoxyribonucleoside triphosphates include, but are not limited to. ddATP. ddCTP. ddGTP, ddlTP, and ddTTP. According to the present invention, a "nucleotide" may be unlabeled or detectably labeled by well known techniques. Detectable labels include, for example, radioactive isotopes, fluorescent labels, chemiluminescent labels, bioluminescent labels and enzyme labels. Thermostable. As used herein "thermostable" refers to a DNA polymerase which is resistant to inactivation by heat. DNA polymerases synthesize the formation of a DNA molecule complementary to a single-stranded DNA template by extending a primer in the 5'-to-3' direction. This activity for mesophilic DNA polymerases may be inactivated by heat treatment. For example, T5 DNA polymerase activity is totally inactivated by exposing the enzyme to a temperature of 90 °C for 30 seconds. As used herein, a thermostable DNA polymerase activity is more resistant to heat inactivation than a mesophilic DNA polymerase. However, a thermostable DNA polymerase does not mean to refer to an enzyme which is totally resistant to heat inactivation and thus heat treatment may reduce the DNA polymerase activity to some extent. A thermostable DNA polymerase typically will also have a higher optimum temperature than mesophilic DNA polymerases.

Hybridization. The terms "hybridization" and "hybridizing" refers to the pairing of two complementary single-stranded nucleic acid molecules (RNA and/or DNA) to give a double-stranded molecule. As used herein, two nucleic

acid molecules may be hybridized, although the base pairing is not completely complementary. Accordingly, mismatched bases do not prevent hybridization of two nucleic acid molecules provided that appropriate conditions, well known in the art, are used. 3'-to-5' Exonuclease Activity. "3'-to-5' exonuclease activity" is an enzymatic activity well known to the art. This activity is often associated with DNA polymerases, and is thought to be involved in a DNA replication "editing" or correction mechanism.

A "DNA polymerase substantially reduced in 3'-to-5' exonuclease activity" is defined herein as either ( 1 ) a mutated DNA polymerase that has about or less than 10%. or preferably about or less than 1%, ofthe 3'-to-5' exonuclease activity of the coπesponding unmutated, wild-type enzyme, or (2) a DNA polymerase having a 3'-to-5' exonuclease specific activity which is less than about 1 unit/mg protein, or preferably about or less than 0.1 units/mg protein. A unit of activity of 3 '-to-5 ' exonuclease is defined as the amount of activity that solubilizes 10 nmoles of substrate ends in 60 min. at 37 °C, assayed as described in the "BRL 1989 Catalogue & Reference Guide", page 5, with Hhal fragments of lambda DNA 3 '-end labeled with [ ³ H]dTTP by terminal deoxynucleotidyl transferase (TdT). Protein is measured by the method of Bradford, Anal. Biochem. 72:248 (1976). As a means of comparison, natural, wild-type T5-DNA polymerase (DNAP) or T5-DNAP encoded by pTTQ19-T5-2 has a specific activity of about 10 units/mg protein while the DNA polymerase encoded by pTTQ19-T5-2(Exo ^" ) (U.S. 5,270,179) has a specific activity of about 0.0001 units/mg protein. or 0.001%) of the specific activity of the unmodified enzyme, a 10 ⁵ -fold reduction.

5'-to-3' Exonuclease Activity. "5'-to-3' exonuclease activity" is also an enzymatic activity well known in the art. This activity is often associated with DNA polymerases. such as E. coli Poll and PolIII.

A "DNA polymerase substantially reduced in 5'-to-3' exonuclease activity" is defined herein as either ( 1 ) a mutated DNA polymerase that has about

or less than 10%. or preferably about or less than 1%, ofthe 5'-to-3' exonuclease activity of the coπesponding unmutated, wild-type enzyme, or (2) a DNA polymerase having 5'-to-3' exonuclease specific activity which is less than about 1 unit mg protein. or preferably about or less than 0.1 units/mg protein. Both of the 3'-to-5' and 5'-to-3' exonuclease activities can be observed on sequencing gels. Active 5 '-to-3 ' exonuclease activity will produce nonspecific ladders in a sequencing gel by removing nucleotides from the 5'-end of the growing primers. 3'-to-5' exonuclease activity can be measured by following the degradation of radiolabeled primers in a sequencing gel. Thus, the relative amounts of these activities, e.g. by comparing wild-type and mutant polymerases. can be determined with no more than routine experimentation.

1. Cloning and Expression of Thermotoga DNA Polymerases

The Thermotoga DNA polymerase ofthe invention can be isolated from any strain of Thermotoga which produces a DNA polymerase. The prefeπed strain to isolate the gene encoding Thermotoga DNA polymerase ofthe present invention is Thermotoga neapolitana (Tne) and Thermotoga maritima (Tma). The most prefeπed Thermotoga neapolitana for isolating the DNA polymerase of the invention was isolated from an African continental solfataric spring (Windberger et al. Arch. Microbiol. 757:506-512 (1989) and may be obtained from Deutsche Sammlung von Microorganismen und Zellkulturen GmbH

(DSMZ; German Collection of Microorganisms and Cell Culture) Mascheroder Weg lb D-38124 Braunschweig, Germany, as Deposit No. 5068 (deposited December 13. 1988).

To clone a gene encoding a Thermotoga DNA polymerase of the invention, isolated DNA which contains the polymerase gene obtained from

Thermotoga cells, is used to construct a recombinant DNA library in a vector. Any vector, well known in the art, can be used to clone the wild type or mutant Thermotoga DNA polymerase ofthe present invention. However, the vector used

must be compatible with the host in which the recombinant DNA library will be transformed.

Prokaryotic vectors for constructing the plasmid library include plasmids such as those capable of replication in E. coli such as, for example, pBR322, ColΕl, pSClOl, pUC-vectors (pUC18, pUC19, etc.: In: Molecular Cloning, A

Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1982); and Sambrook et al, In: Molecular Cloning A Laboratory Manual (2d ed.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989)). Bacillus plasmids include pC194, pC221, pC217. etc. Such plasmids are disclosed by Glyczan, T. In: 77ze Molecular Biology Bacilli,

Academic Press, York (1982), 307-329. Suitable Streptomyces plasmids include pIJlOl (Kendall et al, J. Bacteriol 169:4177-4183 (1987)). Pseudomonas plasmids are reviewed by John et al, (Rad. Insec. Dis. 5:693-704 (1986)), and Igaki, (Jpn. J. Bacteriol. 33:729-742 (1978)). Broad-host range plasmids or cosmids, such as pCP13 (Darzins and Chakrabarbary, J. Bacteriol. 759:9-18,

1984) can also be used for the present invention. The prefeπed vectors for cloning the genes ofthe present invention are prokaryotic vectors. Preferably, pCP13 and pUC vectors are used to clone the genes ofthe present invention.

The prefeπed host for cloning the wild type or mutant DNA polymerase genes ofthe invention is a prokaryotic host. The most prefeπed prokaryotic host is E. coli. However, the wild type or mutant DNA polymerase genes of the present invention may be cloned in other prokaryotic hosts including, but not limited to, Escherichia, Bacillus, Streptomyces, Pseudomonas, Salmonella, Serratia, and Proteus. Bacterial hosts of particular interest include E. coli DH10B, which may be obtained from Life Technologies, Inc. (LTI)

(Gaithersburg, MD).

Eukaryotic hosts for cloning and expression of the wild type or mutant DNA polymerases ofthe present invention include yeast, fungi, and mammalian cells. Expression ofthe desired DNA polymerase in such eukaryotic cells may require the use of eukaryotic regulatory regions which include eukaryotic

promoters. Cloning and expressing the wild type or mutant DNA polymerase gene of the invention in eukaryotic cells may be accomplished by well known techniques using well known eukaryotic vector systems.

Once a DNA library has been constructed in a particular vector, an appropriate host is transformed by well known techniques. Transformed colonies are plated at a density of approximately 200-300 colonies per petri dish. Colonies are then screened for the expression of a heat stable DNA polymerase by transferring transformed E. coli colonies to nitrocellulose membranes. After the transfeπed cells are grown on nitrocellulose (approximately 12 hours), the cells are lysed by standard techniques. and the membranes are then treated at 95 °C for

5 minutes to inactivate the endogenous E coli enzyme. Other temperatures may be used to inactivate the host polymerases depending on the host used and the temperature stability of the DNA polymerase to be cloned. Stable DNA polymerase activity is then detected by assaying for the presence of DNA polymerase activity using well known techniques. Sagner et al, Gene

97:119-123 (1991). which is hereby incoφorated by reference in its entirety. The gene encoding a DNA polymerase ofthe present invention can be cloned using the procedure described by Sagner et al. supra.

The recombinant host containing the wild type gene encoding Tne DNA polymerase. E. coli DH10B (pUC-Tne), was deposited on September 30, 1994. with the Agricultural Research Culture Collection (NRRL), 1815 N. University Street. Peoria, IL 61604 USA as Deposit No. NRRL B-21338. The gene encoding Tma DNA polymerase has also been cloned and sequenced (U.S. Patent 5,374,553, which is expressly incoφorated by reference in its entirety). If the Thermotoga (e.g., Tne or Tma) DNA polymerase has 3'-to-5' exonuclease activity, this activity may be reduced, substantially reduced, or eliminated by mutating the DNA polymerase gene. Such mutations include point mutations, frame shift mutations, deletions and insertions. Preferably, the region ofthe gene encoding the 3'-to-5' exonuclease activity is mutated or deleted using

techniques well known in the art (Sambrook et al. , (1989) in: Molecular Cloning, A Laboratory Manual (2nd Ed.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor. N.Y.).

The 3'-to-5' exonuclease activity can be reduced or impaired by creating site specific mutants within the 3 '-5' exonuclease domain. See infra. In a specific embodiment ofthe invention Asp ³²³ of Tne DNA polymerase (SEQ ID NO. 3) is changed to any amino acid, preferably to Ala ³²³ to substantially reduce 3'-to-5' exonuclease activity. In another specific embodiment ofthe invention. Asp ³²³ of Tma may be changed to any other amino acid, preferably to Ala to substantially reduce 3'-to-5' exonuclease activity.

The 5 '-3' exonuclease activity of the DNA polymerase can be reduced or eliminated by mutating the DNA polymerase gene. Such mutations include point mutations, frame shift mutations, deletions, and insertions. Preferably, the region of the gene encoding the 5 '-3' exonuclease activity is deleted using techniques well known in the art. In embodiments of this invention, any one of six conserved amino acids that are associated with the 5 '-3' exonuclease activity can be mutated. Examples of these conserved amino acids with respect to Tne DNA polymerase include Asp ⁸ , Glu ¹¹² , Asp" ⁴ , Asp" ⁵ , Asp ¹³⁷ , and Asp ¹³⁹ . Other possible sites for mutation are: Gly ¹⁰² , Gly ¹⁸⁷ and Gly ¹⁹⁵ . The present invention is directed broadly to mutations of DNA polymerases that result in the reduction or elimination of 5 '-3' exonuclease activity. Other particular mutations conespond to the following amino acids.

E. coli poll: Asp ¹³ , Glu ¹¹³ , Asp ¹¹⁵ , Asp" ⁶ , Asp ¹³⁸ , and Asp ¹⁴⁰ Taq pol: Asp ¹⁸ , Glu" ⁷ , Asp" ⁹ , Asp ¹²⁰ , Asp ¹⁴² , and Asp ¹⁴⁴ Tma pol: Asp ⁸ , Glu" ² , Asp" ⁴ , Asp" ⁵ , Asp ¹³⁷ , and Asp ¹³⁹

Amino acid residues of Taq DNA polymerase are as numbered in U.S. 5,079,352.

Amino acid residues oϊ Thermotoga maritima (Tma) DNA polymerase are numbered as in U.S. Patent No. 5,374,553.

By comparison to the amino acid sequence of other DNA polymerases, the coπesponding sites can easily be located and the DNA mutanigized to prepare a coding sequence for the coπesponding DNA polymerase which lacks the 5 '-3' exonuclease activity. Examples of other DNA polymerases that can be so mutated include:

Enzyme or source Mutation positions

Streptococcus pneumoniae Asp ¹⁰ , Glu" ⁴ , Asp" ⁶ , Asp" ⁷ , Asp ¹³⁹ , Asp ¹⁴¹

Thermus flavus Asp ¹⁷ , Glu" ⁶ , Asp" ⁸ , Asp" ⁹ , Asp ¹⁴¹ , Asp ¹⁴³

Thermus thermophilus Asp ¹⁸ , Glu" ⁸ , Asp ¹²⁰ , Asp ¹²¹ , Asp' ⁴³ , Asp ¹⁴⁵

Deinococcus radiodwans Asp ¹⁸ , Glu" ⁷ , Asp" ⁹ , Asp' ²⁰ , Asp ¹⁴² , Asp ¹⁴⁴

Bacillus caldotenax Asp ⁹ , Glu ¹⁰⁹ , Asp ¹ ", Asp" ² , Asp ¹³⁴ , Asp ¹³⁶

Coordinates of S. pneumoniae, T flavus. D. radiodurans, B. caldotenax were obtained from Gutman and Minton. Coordinates of T. thermophilus were obtained from International Patent No. WO 92/06200.

To abolish the 5 '-3' exonuclease activity, amino acids are selected which have different properties. For example, an acidic amino acid such as Asp may be changed to a basic, neutral or polar but uncharged amino acid such as Lys, Arg, His (basic); Ala, Val, Leu, He, Pro, Met, Phe, Tφ (neutral); or Gly, Ser, Thr, Cys, Tyr, Asn or Gin (polar but uncharged). Glu may be changed to Asp, Ala, Val Leu, He. Pro, Met, Phe, Tφ, Gly, Ser, Thr, Cys, Tyr, Asn or Gin. Specifically, the Ala substitution in the coπesponding position is expected to abolish 5'-exo activity.

Preferably, oligonucleotide directed mutagenesis is used to create the mutant DNA polymerase which allows for all possible classes of base pair changes at any determined site along the encoding DNA molecule. In general, this technique involves annealing a oligonucleotide complementary (except for

one or more mismatches) to a single stranded nucleotide sequence coding for the DNA polymerase of interest. The mismatched oligonucleotide is then extended by DNA polymerase, generating a double stranded DNA molecule which contains the desired change in sequence on one strand. The changes in sequence can of course result in the deletion, substitution, or insertion of an amino acid. The double stranded polynucleotide can then be inserted into an appropriate expression vector, and a mutant polypeptide can thus be produced. The above- described oligonucleotide directed mutagenesis can of course be carried out via PCR. In other embodiments, the entire 5 ' - 3 ' exonuclease domain of the DNA polymerase can be deleted by proteolytic cleavage or by genetic engineering. For example, a unique SphI restriction site can be used to obtain a clone devoid of nucleotides encoding the 219 amino terminal amino acids of Tne DNA polymerase. Examples of such a clone are pTTQTne535FY and pTTQTne5FY. Alternatively, less than the 219 amino terminal amino acids may be removed, for example, by treating the DNA coding for the Tne DNA polymerase with an exonuclease, isolating the fragments, ligating the fragments into a cloning vehicle, transfecting cells with the cloning vehicle, and screening the transformants for DNA polymerase activity and lack of 5 '-3' exonuclease activity, with no more than routine experimentation.

Thermotoga DNA polymerase mutants can also be made to render the polymerase non-discriminating against non-natural nucleotides such as dideoxynucleotides. Changes within the O-helix of Thermotoga polymerases, such as other point mutations, deletions, and insertions, can be made to render the polymerase non-discriminating. By way of example, one Tne DNA polymerase mutant having this property substitutes a nonnatural amino acid such as Tyr for

Phe at amino acid 67 as numbered in Figs. 5A and 5B, and 730 of SEQ ID NO:3.

The O-helix region is a 14 amino acid sequence coπesponding to amino acids 722-735 of SEQ ID NO:3 or amino acids 59-72 as numbered in Figs 5A and 5B. The O-helix may be defined as RXXXKXXXFXXXYX, wherein X is any

amino acid. The most important amino acids in conferring discriminatory activity include Arg, Lys and Phe. Amino acids which may be substituted for Arg at positions 722 are selected independently from Asp, Glu, Ala, Val Leu, lie, Pro, Met, Phe, Tφ, Gly, Ser, Thr, Cys, Tyr, Gin, Asn, Lys and His. Amino acids that may be substituted for Phe at position 730 include Lys, Arg, His, Asp, Glu, Ala,

Val, Leu, He, Pro, Met, Tφ, Gly, Ser. Thr, Cys, Tyr, Asn or Gin. Amino acids that may be substituted for Lys at position 726 of SEQ ID NO: 3 include Tyr, Arg, His, Asp, Glu, Ala, Val, Leu, He, Pro, Met, Tφ, Gly, Ser, Thr, Cys, Phe, Asn or Gin. Prefeπed mutants include Tyr ⁷³⁰ , Ala ⁷³⁰ , Ser ⁷³⁰ and Thr ⁷³⁰ . Such Tne mutants may be prepared by well known methods of site directed mutagenesis as described herein. See also Example 10.

The coπesponding mutants can also be prepared from Tma DNA polymerase, including Arg ⁷²² , Lys ⁷²⁶ and Phe ⁷³⁰ . Most prefered mutants include Phe ⁷³⁰ to Tyr ⁷³⁰ , Ser ⁷³⁰ , Thr ⁷³⁰ and Ala ⁷³⁰ .

2. Enhancing Expression of Thermotoga DNA Polymerase

To optimize expression of the wild type or mutant Thermotoga DNA polymerases ofthe present invention. inducible or constitutive promoters are well known and may be used to express high levels of a polymerase structural gene in a recombinant host. Similarly, high copy number vectors, well known in the art, may be used to achieve high levels of expression. Vectors having an inducible high copy number may also be useful to enhance expression of Thermotoga DNA polymerase in a recombinant host.

To express the desired structural gene in a prokaryotic cell (such as, E. coli, B. subtilis, Pseudomonas, etc.), it is necessary to operably link the desired structural gene to a functional prokaryotic promoter. However, the natural

Thermotoga promoter may function in prokaryotic hosts allowing expression of the polymerase gene. Thus, the natural Thermotoga promoter or other promoters may be used to express the DNA polymerase gene. Such other promoters may

be used to enhance expression and may either be constitutive or regulatable (i.e., inducible or derepressible) promoters. Examples of constitutive promoters include the int promoter of bacteriophage λ, and the bla promoter of the β-lactamase gene of pBR322. Examples of inducible prokaryotic promoters include the major right and left promoters of bacteriophage λ (P _R and PJ, trp, ree A, lacL, lacl. tet, gal, tre, and tac promoters of E. coli. The B. subtilis promoters include α-amylase (Ulmanen et al, J. Bacteriol 162:176-182 (1985)) and Bacillus bacteriophage promoters (Gryczan. T., In: The Molecular Biology Of Bacilli, Academic Press, New York (1982)). Streptomyces promoters are described by Ward et al, Mol. Gen. Genet. 203:468478 (1986)). Prokaryotic promoters are also reviewed by Glick, J Ind. Microbiol. 1:277-282 (1987); Cenatiempto, Y., Biochimie 68:505-516 (1986); and Gottesman, Ann. Rev. Genet. 75:415-442 (1984). Expression in a prokaryotic cell also requires the presence of a ribosomal binding site upstream of the gene-encoding sequence. Such ribosomal binding sites are disclosed, for example, by Gold et al, Ann. Rev.

Microbiol. 35:365404 (1981).

To enhance the expression of Thermotoga (e.g., 77ze and Tma) DNA polymerase in a eukaryotic cell, well known eukaryotic promoters and hosts may be used. Preferably, however, enhanced expression of Thermotoga DNA polymerase is accomplished in a prokaryotic host. The prefeπed prokaryotic host for overexpressing this enzyme is E. coli.

3. Isolation and Purification of Thermotoga DNA Polymerase

The enzyme(s) ofthe present invention (Thermotoga DNA polymerases and mutants thereof) is preferably produced by fermentation ofthe recombinant host containing and expressing the cloned DNA polymerase gene. However, the wild type and mutant DNA polymerases ofthe present invention may be isolated from any Thermotoga strain which produces the polymerase of the present invention. Fragments of the polymerase are also included in the present

invention. Such fragments include proteolytic fragments and fragments having polymerase activity.

Any nutrient that can be assimilated by Thermotoga or a host containing the cloned Thermotoga DNA polymerase gene may be added to the culture medium. Optimal culture conditions should be selected case by case according to the strain used and the composition ofthe culture medium. Antibiotics may also be added to the growth media to insure maintenance of vector DNA containing the desired gene to be expressed. Culture conditions for Thermotoga neapolitana have, for example, been described by Huber et al, Arch. Microbiol. 144:324-333 (1986). Media formulations are also described in DSM or ATCC

Catalogs and Sambrook et al, In: Molecular Cloning, a Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989).

Thermotoga and recombinant host cells producing the DNA polymerase of this invention can be separated from liquid culture, for example, by centrifugation. In general, the collected microbial cells are dispersed in a suitable buffer, and then broken down by ultrasonic treatment or by other well known procedures to allow extraction of the enzymes by the buffer solution. After removal of cell debris by ultracentrifugation or centrifugation, the DNA polymerase can be purified by standard protein purification techniques such as extraction, precipitation, chromatography, affinity chromatography, electrophoresis or the like. Assays to detect the presence ofthe DNA polymerase during purification are well known in the art and can be used during conventional biochemical purification methods to determine the presence of these enzymes.

4. Uses of Thermotoga DNA Polymerase

The wild type and mutant Thermotoga DNA polymerases (e.g., Tma and

Tne) ofthe present invention may be used in well known DNA sequencing, DNA labeling, DNA amplification and cDNA synthesis reactions. Thermotoga DNA polymerase mutants devoid of or substantially reduced in 3'- 5' exonuclease

activity, devoid of or substantially reduced in 5'-* 3' exonuclease activity, or containing one or mutations in the O-helix that make the enzyme nondiscriminatory for dNTPs and ddNTPs (e.g., a Phe ⁷³⁰ →Tyr ⁷³⁰ mutation of SEQ ID NO: 3) are especially useful for DNA sequencing, DNA labeling, and DNA amplification reactions and cDNA synthesis. Moreover, Thermotoga DNA polymerase mutants containing two or more of these properties are also especially useful for DNA sequencing, DNA labeling, DNA amplification or cDNA synthesis reactions. As is well known, sequencing reactions (isothermal DNA sequencing and cycle sequencing of DNA) require the use of DNA polymerases. Dideoxy-mediated sequencing involves the use of a chain-termination technique which uses a specific polymer for extension by DNA polymerase, a base-specific chain terminator and the use of polyacrylamide gels to separate the newly synthesized chain-terminated DNA molecules by size so that at least a part ofthe nucleotide sequence of the original DNA molecule can be determined. Specifically, a DNA molecule is sequenced by using four separate DNA sequence reactions, each of which contains different base-specific terminators. For example, the first reaction will contain a G-specific terminator, the second reaction will contain a T-specific terminator, the third reaction will contain an A-specific terminator, and a fourth reaction may contain a C-specific terminator. Prefeπed terminator nucleotides include dideoxyribonucleoside triphosphates

(ddNTPs) such as ddATP. ddTTP, ddGTP, ddlTP and ddCTP. Analogs of dideoxyribonucleoside triphosphates may also be used and are well known in the art.

When sequencing a DNA molecule, ddNTPs lack a hydroxyl residue at the 3' position of the deoxyribose base and thus, although they can be incoφorated by DNA polymerases into the growing DNA chain, the absence of the 3 '-hydroxy residue prevents formation of the next phosphodiester bond resulting in termination of extension ofthe DNA molecule. Thus, when a small amount of one ddNTP is included in a sequencing reaction mixture, there is competition between extension of the chain and base-specific termination

resulting in a population of synthesized DNA molecules which are shorter in length than the DNA template to be sequenced. By using four different ddNTPs in four separate enzymatic reactions, populations of the synthesized DNA molecules can be separated by size so that at least a part of the nucleotide sequence ofthe original DNA molecule can be determined. DNA sequencing by dideoxy-nucleotides is well known and is described by Sambrook et al, In: Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989). As will be readily recognized, the Thermotoga DNA polymerases and mutants thereof ofthe present invention may be used in such sequencing reactions.

As is well known, detectably labeled nucleotides are typically included in sequencing reactions. Any number of labeled nucleotides can be used in sequencing (or labeling) reactions, including, but not limited to, radioactive isotopes, fluorescent labels, chemiluminescent labels, bioluminescent labels, and enzyme labels. It has been discovered that the wild type and mutant DNA polymerase of the present invention may be useful for incoφorating αS nucleotides ([αS]dATP, [αS]dTTP, [αSjdCTP and [αSJdGTP) during sequencing (or labeling) reactions. For example, [α ³⁵ S]dATP, a commonly used detectably labeled nucleotide in sequencing reactions, is incoφorated three times more efficiently with the Tne DNA polymerase ofthe present invention, than with Taq

DNA polymerase. Thus, the enzyme of the present invention is particularly suited for sequencing or labeling DNA molecules with [α ³⁵ S]dNTPs.

Polymerase chain reaction (PCR), a well known DNA amplification technique, is a process by which DNA polymerase and deoxyribonucleoside triphosphates are used to amplify a target DNA template. In such PCR reactions, two primers, one complementary to the 3 ' termini (or near the 3 '-termini) ofthe first strand of the DNA molecule to be amplified, and a second primer complementary to the 3' termini (or near the 3 '-termini) ofthe second strand of the DNA molecule to be amplified, are hybridized to their respective DNA strands. After hybridization, DNA polymerase, in the presence of

deoxyribonucleoside triphosphates, allows the synthesis of a third DNA molecule complementary to the first strand and a fourth DNA molecule complementary to the second strand ofthe DNA molecule to be amplified. This synthesis results in two double stranded DNA molecules. Such double stranded DNA molecules may then be used as DNA templates for synthesis of additional DNA molecules by providing a DNA polymerase, primers, and deoxyribonucleoside triphosphates. As is well known, the additional synthesis is carried out by "cycling" the original reaction (with excess primers and deoxyribonucleoside triphosphates) allowing multiple denaturing and synthesis steps. Typically, denaturing of double stranded DNA molecules to form single stranded DNA templates is accomplished by high temperatures. The wild type and mutant Thermotoga DNA polymerases of the present invention are heat stable DNA polymerases, and thus will survive such thermal cycling during DNA amplification reactions. Thus, the wild type and mutant DNA polymerases ofthe invention are ideally suited for PCR reactions, particularly where high temperatures are used to denature the DNA molecules during amplification.

The Thermotoga DNA polymerase and mutants ofthe present invention (e.g. Tne and Tma) may also be used to prepare cDNA from mRNA templates. See, U.S. Patent Nos. 5,405,776 and 5,244,797, the disclosures of which are explicitly incoφorated by reference herein. Thus, the invention also relates to a method of preparing cDNA from mRNA, comprising

(a) contacting mRNA with an oligo(dT) primer or other complementary primer to form a hybrid, and

(b) contacting said hybrid formed in step (a) with the Thermotoga DNA polymerase or mutant of the invention and the four dNTPs, whereby a cDNA-RNA hybrid is obtained.

If the reaction mixture is step (b) further comprises an appropriate oligonucleotide which is complementary to the cDNA being produced, it is also possible to obtain dsDNA following first strand synthesis. Thus, the invention

is also directed to a method of preparing dsDNA with the Thermotoga DNA polymerases and mutants thereof of the present invention.

5. Kits

The wild type and mutant Thermotoga DNA polymerases ofthe invention are suited for the preparation of a kit. Kits comprising the wild type or mutant

DNA polymerase(s) may be used for detectably labeling DNA molecules, DNA sequencing, amplifying DNA molecules or cDNA synthesis by well known techniques, depending on the content ofthe kit. See U.S. Patent Nos. 4,962,020, 5,173.411, 4,795,699, 5,498,523, 5,405,776 and 5,244,797. Such kits may comprise a carrying means being compartmentalized to receive in close confinement one or more container means such as vials, test tubes and the like. Each of such container means comprises components or a mixture of components needed to perform DNA sequencing, DNA labeling, DNA amplification, or cDNA synthesis. A kit for sequencing DNA may comprise a number of container means.

A first container means may, for example, comprise a substantially purified sample of Thermotoga DNA polymerases or mutants thereof. A second container means may comprise one or a number of types of nucleotides needed to synthesize a DNA molecule complementary to DNA template. A third container means may comprise one or a number of different types of dideoxynucleoside triphosphates. A fourth container means may comprise pyrophosphatase. In addition to the above container means, additional container means may be included in the kit which comprise one or a number of DNA primers.

A kit used for amplifying DNA will comprise, for example, a first container means comprising a substantially pure mutant or wild type Thermotoga

DNA polymerase ofthe invention and one or a number of additional container means which comprise a single type of nucleotide or mixtures of nucleotides. Various primers may or may not be included in a kit for amplifying DNA.

Kits for cDNA synthesis will comprise a first container means containing the wild type or mutant Tne DNA polymerase ofthe invention, a second container means will contain the four dNTPs and the third container means will contain oligo(dT) primer. See U.S. Patent Nos. 5,405,776 and 5,244,797. Since the Thermotoga DNA polymerases of the present invention are also capable of preparing dsDNA, a fourth container means may contain an appropriate primer complementary to the first strand cDNA.

Of course, it is also possible to combine one or more of these reagents in a single tube. A detailed description of such formulations at working concentrations is described in the patent application entitled "Stable

Compositions for Nucleic Acid Amplification and Sequencing" filed on August

14, 1996, which is expressly incoφorated by reference herein in its entirety.

When desired, the kit ofthe present invention may also include container means which comprise detectably labeled nucleotides which may be used during the synthesis or sequencing of a DNA molecule. One of a number of labels may be used to detect such nucleotides. Illustrative labels include, but are not limited to, radioactive isotopes, fluorescent labels, chemiluminescent labels, bioluminescent labels and enzyme labels.

6. Advantages ofthe Thermotoga DNA Polymerase

Thermotoga DNA polymerases ofthe invention have distinct advantages in DNA sequencing. For example, when using the Tne DNA polymerase mutants ofthe invention in single-extension sequencing, they generate strong, clear ³⁵ S- labeled sequence, increase sequence signal to background ratio, generate ≥500 bases of sequence, reduce false stops in the sequencing ladder, and permit high temperature sequencing reactions. The efficient ³⁵ S incoφoration by the 7>ze

DNA polymerase mutants ofthe invention can reduce template requirement 10- fold, give shaφer bands than ³² P, emit lower energy radiation than ³² P, and have a longer shelflife than ³² P. Further, the Tne polymerase mutants produce longer

sequence reads and gives more accurate sequence inteφretation. In addition, the use of a 70°C reaction temperature with this thermophilic polymerase increases sequencing efficiency of structure-containing and GC-rich templates.

Compared to modified T7 DNA polymerase (Sequenase™), Tne DNA polymerase mutants allow improved sequencing efficiency of structure containing and GC-rich templates, are more forgiving in incubation times for labeling and extensions, and allow one to obtain full length sequence from one-tenth the amount of template. With regard to other polymerases, the Tne DNA polymerase mutants provide, under appropriate reaction conditions, more even band intensities and give longer, more accurate sequence reads, exhibit no weak or absent "dropout" bands, exhibit improved sequencing efficiency of structure containing and GC-rich templates, exhibit no sequence artifacts from templates containing homopolymers, and provide for shorter film exposure and/or less template input due to the efficient ³⁵ S-dNTP incoφoration. With regard to cycle sequencing, the Tne DNA polymerase mutants generate strong, clear ³⁵ S-labeled sequence, they increase sequence signal to background ratio, generate ≥500 bases of sequence, reduce false stops in the sequencing ladder under appropriate conditions, and permit high temperature reactions. The Tne DNA polymerase mutants also allow for highly efficient ³⁵ S dATP incoφoration and therefore shorter film exposures and/or less template input, give shaφer bands than ² P, give off lower energy radiation than ³² P and have a longer shelflife than ³² P. The Tne DNA polymerase mutants also produce longer sequence reads and give more accurate sequence inteφretation. ³² P end labeling of primers generates data with less background from less pure DNA and requires as little as 5 fmole (0.01 μg) of DNA.

With regard to cycle sequencing, compared to the mutant Taq DNA polymerase (ThermoSequenase™), the Tne DNA polymerase mutants generate three times stronger ³⁵ S-labeled sequence without an extra 2 hour cycled labeling step, require no special primer design for ³⁵ S labeling, and allow for sequencing of PCR products directly using any primer. Compared to SequiTherm™, the

mutants of Tne DNA polymerase generate three times stronger ³⁵ S-labeled sequence, give more even band intensities, gives longer and more accurate sequence reads, require less template and less primer, and give no sequence artifacts from templates containing homopolymers. Compared to various other polymerases (e.g. Tth DNA polymerase), the Tne DNA polymerase mutants under appropriate reaction conditions generate three times stronger ³⁵ S-labeled sequence, give more even band intensities, give longer and more accurate sequence reads, give no weak or absent "dropout" bands, improve sequencing efficiency of structure-containing and GC-rich templates, and reduce false stops in sequencing ladders, including through homopolymer regions.

With regard to fluorescent sequencing, the mutants of Tne DNA polymerase readily accept dye primers and dye terminators, increase sequence signal to background ratio, produce fewer ambiguous calls, and generate ≥500 bases of sequence. The Tne DNA polymerase mutants also produce longer sequence read lengths, give more accurate sequence inteφretation, and allow for quantitation of bases in heterologous mixtures. Since the Tne DNA polymerase mutants provide for good incoφoration of dye terminators, such dye terminators can be reduced 500-fold. Further, increased signal improves bases calling, reduces cost and time to sequence, eliminates the need to remove excess dye terminators before gel loading, and produces more even band intensities. The efficient use of dye primers generates data with less background from impure DNA and requires as little as 0.6 μg of dsDNA (double-stranded DNA).

With regard to the use of Thermo Sequenase™ and AmpliTaq FS™ in fluorescent sequencing, the Tne DNA polymerase mutants provide more even band intensities in dye terminator sequencing and give comparable results with dye primers. With regard to SequiTherm™, the Tne DNA polymerase mutants give more even band intensities that give longer, more accurate sequencing reads with both dye terminators and dye primers, use 500-fold less dye terminators, eliminate post reaction clean up of dye terminators, require 10-fold less template, and allow for quantitation of bases in heterologous mixtures using dye primers.

With regard to the use of various other enzymes in fluorescent sequencing, such as AmpliTaq™ and AmpliTaqCS ™ mutant Tne DNA polymerases under appropriate reaction conditions provide more even band intensities and more accurate sequence reads with both dye terminators and dye primers, give no weak or absent "dropout" bands, have lower background and fewer false stops, use 500-fold less dye terminators, eliminate post reaction clean up of dye terminators, require 10-fold less template, and allow for quantitation of bases in heterologous mixtures.

As shown in Fig. 3, Tne DNA polymerase incoφorates α-thio dATP at three times the rate of Taq DNA polymerase. However, suφrisingly, when α-thio dATP is used in place of dATP in sequencing reactions using [α- ³⁵ S]dATP and mutants of Tne DNA polymerase, the resulting sequencing band signal intensity is increased by approximately 8-10 fold. The weak signal seen when dATP is used reflects the mutant DNA polymerase 's strong preference for incoφorating dATP over α-thio dATP from a mixed pool. Attempts to improve signal intensity by merely decreasing the amount of dATP resulted in very poor quality sequence with many false stops. Parallel experiments with [α- ³² P]dATP and low concentrations of dATP produced similar poor quality sequence, indicating that the nucleotide concentration imbalance was causing the enzyme to perform poorly. By using α-thio dATP mixed with [α- ³⁵ S]dATP, the four nucleotide concentrations kept constant without diminishing signal or sequence quality.

Having now generally described the invention. the same will be more readily understood through reference to the following Examples which are provided by way of illustration, and are not intended to be limiting ofthe present invention, unless specified.

Example 1: Bacterial Strains And Growth Conditions

Thermotoga neapolitana DSM No. 5068 was grown under anaerobic conditions as described in the DSM catalog (addition of resazurin. Na ₂ S, and sulfur granules while sparging the media with nitrogen) at 85 °C in an oil bath from 12 to 24 hours. The cells were harvested by filtering the broth through

Whatman #1 filter paper. The supernatant was collected in an ice bath and then centrifuged in a refrigerated centrifuge at 8,000 φms for twenty minutes. The cell paste was stored at -70 °C prior to total genomic DNA isolation.

E. coli strains were grown in 2X LB broth base (Lennox L broth base: GIBCO/BRL) medium. Transformed cells were incubated in SOC (2% tryptone,

0.5% yeast extract, yeast 10 mM NaCl, 2.5 mM KCI, 20mM glucose. lOmM MgCl ₂ , and lOmM MgSO ₄ per liter) before plating. When appropriate antibiotic supplements were 20 mg/1 tetracycline and 100 mg/1 ampicillin. E. coli strain DH10B (Lorow et al. Focus 12:19-20 (1990)) was used as host strain. Competent DH10B may be obtained from Life Technologies, Inc. (LTI)

(Gaithersburg, MD).

Example 2: DNA Isolation

Thermotoga neapolitana chromosomal DNA was isolated from l.lg of cells by suspending the cells in 2.5 ml TNΕ (50mM Tris-HCl, pH 8.0, 50mM NaCl, lOmM ΕDTA) and treated with 1% SDS for 10 minutes at 37°C. DNA was extracted with phenol by gently rocking the lysed cells overnight at 4°C. The next day, the lysed cells were extracted with chloroform: isoamyl alcohol. The resulting chromosomal DNA was further purified by centrifugation in a CsCl density gradient. Chromosomal DNA isolated from the density gradient was extracted three times with isopropanol and dialyzed overnight against a buffer containing 10 mM Tris-HCl (pH 8.0) and 1 mM ΕDTA (TΕ).

Example 3: Construction of Genomic Libraries

The chromosomal DNA isolated in Example 2 was used to construct a genomic library in the plasmid pCP13. Briefly, 10 tubes each containing lOμg of Thermotoga neapolitana chromosomal DNA was digested with 0.01 to 10 units of Sau3Al for 1 hour at 37°C. A portion ofthe digested DNA was tested in an agarose (1.2%) gel to determine the extent of digestion. Samples with less than 50%) digestion were pooled, ethanol precipitated and dissolved in TE. 6.5 μg of partially digested chromosomal DNA was ligated into 1.5 μg of pCP13 cosmid which had been digested with BamEΪ restriction endonuclease and dephosphorylated with calf intestinal alkaline phosphatase. Ligation of the partially digested Thermotoga DNA and BamHl cleaved pCP13 was carried out with T4 DNA ligase at 22 °C for 16 hours. After ligation, about lμg of ligated DNA was packaged using λ-packaging extract (obtained from Life Technologies, Inc., Gaithersburg, MD). DH10B cells (Life Tech. Inc.) were then infected with 100 μl ofthe packaged material. The infected cells were plated on tetracycline containing plates. Serial dilutions were made so that approximately 200 to 300 tetracycline resistant colonies were obtained per plate.

Example 4: Screening for Clones Expressing Thermotoga neapolitana DNA Polymerase

Identification ofthe Thermotoga neapolitana DNA polymerase gene of the invention was cloned using the method of Sagner et al. Gene 97:119-123 (1991) which reference is herein incoφorated in its entirety. Briefly, the E. coli tetracycline resistant colonies from Example 3 were transfeπed to nitrocellulose membranes and allowed to grow for 12 hours. The cells were then lysed with the fumes of chloroform:toluene (1 :1) for 20 minutes and dried for 10 minutes at room temperature. The membranes were then treated at 95 °C for 5 minutes to inactivate the endogenous E. coli enzymes. Surviving DNA polymerase activity

was detected by submerging the membranes in 15 ml of polymerase reaction mix (50 mM Tris-HCl (pH 8.8), 1 mM MgCl ₂ , 3 mM β-mercaptoethanol, 10 μM dCTP, dGTP, dTTP, and 15 μCi of 3,000 Ci/mmol [α ³² P]dATP) for 30 minutes at 65°C. Using autoradiography, three colonies were identified that expressed a

Thermotoga neapolitana DNA polymerase. The cells were grown in liquid culture and the protein extract was made by sonication. The presence of the cloned thermostable polymerase was confirmed by treatment at 90 °C followed by measurement of DNA polymerase activity at 72°C by incoφoration of radioactive deoxyribonucleoside triphosphates into acid insoluble DNA. One of the clones, expressing Tne DNA polymerase, contained a plasmid designated pCP13-32 and was used for further study.

Example 5: Subcloning of Tne DNA polymerase

Since the pCP13-32 clone expressing the Tne DNA polymerase gene contains about 25 kb of T. neapolitana DNA, subcloning a smaller fragment of the Tne polymerase gene was attempted. The molecular weight ofthe Tne DNA polymerase purified from E coli/pC? 13-32 was about 100 kd. Therefore, a

2.5-3.0 kb DNA fragment will be sufficient to code for full-length polymerase.

A second round of Sau3A partial digestion similar to Example 3 was done using pCP 13-32 DNA. In this case, a 3.5 kb region was cut out from the agarose gel, purified by Gene Clean (BIO 101, La Jolla, CA) and ligated into plasmid pSport 1 (Life Technologies, Inc.) which had been linearized with BamHl and dephosphorylated with calf intestinal alkaline phosphatase. After ligation,

DH10B was transformed and colonies were tested for DNA polymerase activity as described in Example 4. Several clones were identified that expressed Tne

DNA polymerase. One ofthe clones (pSpoτt-Tne) containing about 3 kb insert was further characterized. A restriction map ofthe DNA fragment is shown in

Fig. 4. Further, a 2.7 Kb Hindlϊl-Sstl fragment was subcloned into pUC19 to generate pUC19-7we. E. coli/pUC19-Tne also produced Tne DNA polymerase. The Tne polymerase clone was sequenced by methods known in the art. The nucleotide sequence obtained of the 5' end prior to the start ATG is shown in SΕQ ID NO: 1. The nucleotide sequence obtained which encodes carboxy- terminal region of the Tne polymerase is shown in Figs. 5 A and 5B (SΕQ ID NO: 17). When SΕQ ID NO: 17 is translated it does not produce the entire amino acid sequence ofthe Tne polymerase due to frame shift enors generated during the determination ofthe nucleotide sequence. However, an amino acid sequence of the Tne polymerase was obtained by translating all three reading frames of

SΕQ ID NO: 17, comparing these sequences with known polymerase amino acid sequences, and splicing the Tne polymerase sequence together to form the amino acid sequence set forth in SΕQ ID NO: 18. The complete nucleotide sequence coding for Tne is shown in SΕQ ID NO:2 and the complete amino acid sequence is shown in SΕQ ID NO:3.

SΕQ ID NO:3 shows that the Tne sequence has an N-terminal methionine. It is not known with certainty whether the wild type 7>2e protein comprises an N- terminal methionine. It is possible to remove this N-terminal methionine according to methods well known to those of ordinary skill in the art, e.g. with a methionine amino peptidase.

Example 6: Purification of Thermotoga neapolitana DNA Polymerase from E. coli

Twelve grams of E. coli cells expressing cloned Tne DNA polymerase (DH10B/pSport-7«e) were lysed by sonication (four thirty-second bursts with a medium tip at the setting of nine with a Heat Systems Ultrasonics Inc., model 375 sonicator) in 20 ml of ice cold extraction buffer (50 mM Tris HCl (pH 7.4), 8% glycerol. 5 mM mercaptoethanol, 10 mM NaCl, 1 mM ΕDTA, 0.5 mM PMSF). The sonicated extract was heated at 80 °C for 15 min. and then cooled in ice for

5 min. 50 mM KCI and PEI (0.4%>) was added to remove nucleic acids. The extract was centrifuged for clarification. Ammonium sulfate was added to 60%, the pellet was collected by centrifugation and resuspended in 10 ml of column buffer (25 mM Tris-HCl (pH 7.4), 8% glycerol, 0.5% EDTA, 5mM 2-mercaptoethanol, 10 mM KCI). A Blue-Sepharose (Pharmacia) column, or preferably a Toso heparin (Tosohaas) column, was washed with 7 column volumes of column buffer and eluted with a 15 column volume gradient of buffer from 1 OmM to 2 M KCI. Fractions containing polymerase activity were pooled. The fractions were dialyzed against 20 volumes of column buffer. The pooled fractions were applied to a Toso650Q column (Tosohaas). The column was washed to baseline OD ₂₈₀ and elution effected with a linear 10 column volume gradient of 25 mM Tris (pH 7.4), 8% glycerol, 0.5 mM EDTA, 10 mM KCI, 5 mM β-mercaptoethanol to the same buffer plus 650 mM KCI. Active fractions were pooled.

Example 7: Characterization of Purified Tne DNA

Polymerase

Determination of the Molecular Weight of Thermotoga neapolitana DNA Polymerase

The molecular weight of 100 kilodaltons was determined by electrophoresis in a 12.5% SDS gel by the method of Laemmli, U.K., Nature

(Lond.) 227:680-685 (1970). Proteins were detected by staining with Coomassie brilliant blue. A 10 kd protein ladder (Life Technologies, Inc.) was used as a standard.

Method for Measuring Incorporation of [a ³⁵ S]-dATP Relative to -dATP

Incoφoration of [αS]dATP was evaluated in a final volume of 500 μl of reaction mix, which was preincubated at 72 °C for five minutes, containing either a [Η]TTP nucleotide cocktail (100 μM each TTP, dATP, dCTP, dGTP with

[ ³ H]TTP at 90.3 cpm/pmol), a nucleotide cocktail containing [αSjdATP as the only source of dATP (100 μM each [αSjdATP, dCTP, dGTP. TTP with [α ³⁵ S]dATP at 235 cpm/pmol). or a mixed cocktail (50 μM [αS]dATP, 50 μM dATP, 100 μM TTP, 100 μM dCTP. 100 μM dGTP with [ ³⁵ αS] dATP at 118 cpm/pmol and [ ³ H]TTP at 45.2 cpm/pmol) and 50 mM bicine, pH 8.5. 30 mM

MgCl ₂ , 0.25 mg/ml activated salmon sperm DNA, 20% glycerol. The reaction was initiated by the addition of 0.3 units of T. neapolitana DNA polymerase or T. aquaticus DNA polymerase. At the times indicated a 25 μl aliquot was removed and quenched by addition of ice cold EDTA to a final concentration of 83 mM. 20 μl aliquots ofthe quenched reaction samples were spotted onto GF/C filters. Rates of incoφoration were compared and expressed as a ratio of T. neapolitana to T. aquaticus. The incoφoration of [α ³⁵ S]dATP by T neapolitana DNA polymerase was three-fold higher than that of T. aquaticus DNA polymerase.

Example 8: Reverse Transcriptase Activity

(A) _n :(dT) ₁₂ . ₁₈ is the synthetic template primer used most frequently to assay for reverse transcriptase activity of DNA polymerases. It is not specific for retroviral-like reverse transcriptase, however, being copied by many prokaryotic and eukaryotic DNA polymerases (Modak and Marcus, J. Biol. Chem. 252:11-19 (1977); Gerard et al, Biochem. 75:1632-1641 (1974); Spadari and Weissbach, J.

Biol. Chem. 249:5809-5815 (1974)). (A) _n :(dT) _I2 . ₁₈ is copied particularly well by cellular, replicative DNA polymerases in the presence of Mn ^{"^+} , and much less

efficiently in the presence of Mg ^** (Modak and Marcus, J Biol. Chem. 252: 11-19 (1977); Gerard et al, Biochem. 75:1632-1641 (1974); Spadari and Weissbach, J Biol. Chem. 249:5809-5815 (1974)). In contrast, most cellular, replicative DNA polymerases do not copy the synthetic template primer (C) _n :(dG) _I2 . ₁₈ efficiently in presence of either Mn ^* ' or Mg ⁺⁺ , but retroviral reverse transcriptases do.

Therefore, in testing for the reverse transcriptase activity of a DNA polymerase with synthetic template primers, the stringency of the test increases in the following manner from least to most stringent: (A) _π :(dT) ₁₂ .ι ₈ (Mn ⁺⁺ ) < (A) _n :(dT) ₁₂ . ₁₈ (MgA « (C) _n :(dG) _I2 . ₁₈ (Mn ⁺⁺ ) < (C) _π :(dG) ₁₂ . ₁₈ (Mg ⁺⁺ ). The reverse transcriptase activity of Tne DNA polymerase was compared with Thermus thermophilus (Tth) DNA polymerase utilizing both (A) _n :(dT) ₂₀ and (C) _π :(dG) _I2 . _Ig . Reaction mixtures (50 μl) with (A) _n :(dT) ₂₀ contained 50 mM Tris- HCl (pH 8.4), 100 μM (A) _n , 100 μM (dT) ₂₀ , and either 40 mM KCI, 6 mM MgCl ₂ , 10 mM dithiothreitol, and 500 μM [ΗjdTTP (85 cpm pmole), or 100 mM KCI, 1 mM MnCl,. and 200 μM [ ³ H]dTTP (92 cpm/pmole). Reaction mixtures

(50 μl) with (C) _n :(dG) _12-18 contained 50 mM Tris-HCl (pH 8.4), 60 μM (C)„, 24 μM (dG).,.- ₈ , and either 50 mM KCI, 10 mM MgCl,, 10 mM dithiothreitol, and 100 μM [ ³ H]dGTP (132 cpm/pmole), or 100 mM KCI. 0.5 mM MnCl,, and 200 μM [ ³ H]dGTP (107 cpm/pmole). Reaction mixtures also contained either 2.5 units of the Tth DNA polymerase (Perkin-Elmer) or 2.5 units of the Tne DNA polymerase. Incubations were at 45 °C for 10 min followed by 75 °C for 20 min.

The table shows the results of determining the relative levels of incoφoration of Tne and Tth DNA polymerase with (A) _n :(dT) ₂₀ and (C) _n :(dG) _12.18 in the presence of Mg ⁺⁺ and Mn ⁺⁺ . Tne DNA polymerase appears to be a better reverse transcriptase than Tth DNA polymerase under reaction conditions more specific for reverse transcriptase, i.e., in the presence of (A) _n :(dT), ₀ with Mg ⁺⁺ and (C) _n :(dG) _12.I8 with Mn ⁺⁺ or Mg ⁺⁺ .

DNA Polymerase Activity of Tth and Tne DNA Polymerase with (A)„:(dT) ₂₀ and (C) _n :(dG) . _lg

DNA Polymerase Activity (pMoles Complementary [ ³ H]dNTP Incoφorated)

Enzyme

(A) _n :(dT) ₂₀ (C) _n :(dG) Mg Mn* ^*

Tne 161.8 188.7 0.6 4.2 Tth 44.8 541.8 0 0.9

Example 9: Construction of Thermotoga Neapolitana 3'-to-5'

Exonuclease Mutant

The amino acid sequence of portions of the Tne DNA polymerase was compared with other known DNA polymerases such as E. coli DNA polymerase 1, Taq DNA polymerase, T5 DNA polymerase, and T7 DNA polymerase to localize the regions of 3'-to-5' exonuclease activity, and the dNTP binding domains within the DNA polymerase. One ofthe 3'-to-5' exonuclease domains was determined based on the comparison ofthe amino acid sequences of various DNA polymerases (Blanco. L., et al. Gene 112: 139-144 (1992); Braithwaite and Ito, Nucleic Acids Res. 21 : 787-802 (1993)) is as follows:

Tne 318 PSFALD'LETSS 328 (SEQ ID NO: 4)

Pol I 350 PVFAFDTETDS 360 (SEQ ID NO:5; Braithwaite and Ito, supra)

T5 133 GPVAFDSETSA 143 (SEQ ID NO:6; Braithwaite and Ito, supra)

T7 1 MIVSDIEANA 10 (SEQ ID NO:7; Braithwaite and Ito, supra).

As a first step to make the Tne DNA polymerase devoid of 3 '-5' exonuclease activity, a 2kb Sph fragment from pSport-Tne was cloned into M13mpl9 (LTI, Gaithersburg, MD). The recombinant clone was selected in E. coli DH5αFTQ (LTI, Gaithersburg, MD). One of the clones with the proper insert was used to isolate uracilated single-stranded DNA by infecting E. coli

CJ236 (Biorad, California) with the phage particle obtained from E. coli DH5αFTQ. An oligonucleotide, GA CGT TTC AAG CGC TAG GGC AAA AGA (SΕQ ID NO:8) was used to perform site directed mutagenesis. This site- directed mutagenesis converted Asp ³²³ (indicated as * above) to Ala ³²³ . An Eco47III restriction site was created as part of this mutagenesis to facilitate screening ofthe mutant following mutagenesis. The mutagenesis was performed using a protocol as described in the Biorad manual (1987) except T7 DNA polymerase was used instead of T4 DNA polymerase (USB, Cleveland, OH). The mutant clones were screened for the Eco47III restriction site that was created in the mutagenic oligonucleotide. One of the mutants having the created

Ecø47III restriction site was used for further study. The mutation Asp ³²³ to Ala ³²³ has been confirmed by DNA sequencing.

To incoφorate the 3'-to-5' exonuclease mutation in an expression vector, the mutant phage was digested with SphI and Hindlll. A 2 kb fragment containing the mutation was isolated. This fragment was cloned in pUC-Tne to replace the wild type fragment. See Figure 6A. The desired clone. pUC-Tne (3 '-5'), was isolated. The presence ofthe mutant sequence was confirmed by the presence ofthe unique Eco47lll site. The plasmid was then digested with Sstl and Hindlll. The entire mutant polymerase gene (2.6 kb) was purified and cloned into Sstl and Hindlll digested pTrc99 expression vector (Pharmacia, Sweden).

The clones were selected in DH10B (LTI, Gaithersburg, MD). The resulting plasmid was designated pTrcTne35. See Figure 6B. This clone produced active heat stable DNA polymerase.

Example 10: Phenylalanine to Tyrosine Mutant

As discussed supra, the polymerase active site including the dNTP binding domain is usually present at the carboxyl terminal region of the polymerase. The sequence ofthe 7>7e polymerase gene suggests that the amino acids that presumably contact and interact with the dNTPs are present within the

694 bases starting at the internal BamHl site. See Figure 4 and Figs. 5 A and 5B. This conclusion is based on homology with a prototype polymerase E coli DNA polymerase 1. See Polisky et al.. J. Biol. Chem. 265:14579-14591 (1990). The sequence of the carboxyl terminal portion of the polymerase gene is shown in Figs. 5A and 5B. Based upon this sequence, it is possible to compare the amino acid sequence within the O-helix for various polymerases. The complete sequence ofthe DNA polymerase is shown in SΕQ ID NO:3. The coπesponding O-helix region band on the sequence in Figs. 5A and 5B includes amino acids 59 to 72.

Tne 722 RRVGKMVNFSIIYG 735 (SΕQ ID NO:9)

Pol l 754 RRSAKAINFGLIYG 767 (SEQ ID NO:10)

T5 562 RQAAKAITFGILYG 575 (SEQ ID NO: 11)

T7 518 RDNAKTFIYGFLYG 531 (SEQ ID NO: 12)

Taq 659 RRAAKTINFGVLYG 672 (SEQ ID NO: 13)

It was shown that by replacing the phenylalanine residue of Taq DNA polymerase, the polymerase becomes non-discriminating against non-natural nucleotides such as dideoxynucleotides. See application Serial No. 08/525,087 entitled "Mutant DNA Polymerases and Use Thereof of Deb K. Chatterjee, filed September 8, 1995, specifically incoφorated herein by reference. The mutation was based on the assumption that T7 DNA polymerase contains a tyrosine residue in place of the phenylalanine, and T7 DNA polymerase is non-discriminating against dideoxynucleotides. The coπesponding residue, Phe ⁷⁶² of E. coli Poll is

an amino acid that directly interacts with nucleotides. (Joyce and Steitz, Ann. Rev. Biochem. 63:777-822 (1994); Astake, M .. J. Biol. Chem. 270:1945-1954 (1995)). A similar mutant of Tne DNA polymerase was prepared.

In order to change Phe ⁷³⁰ ofthe Tne polymerase to a Tyr ⁷³⁰ as numbered in SEQ ID NO:3, site directed mutagenesis was performed using the oligonucleotide GTA TAT TAT AGA GTA GTT AAC CAT CTT TCC A. (SEQ ID NO: 14). As part of this oligonucleotide directed mutagenesis, a Hpal restriction site was created in order to screen mutants easily. The same uracilated single-stranded DNA and mutagenesis procedure described in Example 9 were used for this mutagenesis. Following mutagenesis. the mutants were screened for the Hpal site. Mutants with the desired Hpal site were used for further study. The mutation has been confirmed by DNA sequencing.

The Phe ⁷³⁰ to Tyr ⁷³⁰ mutation was incoφorated into pUC-7>7e by replacing the wild type SphI -Hindlll fragment with the mutant fragment obtained from the mutant phage DNA. The presence of the desired clone, pUC-TneFY, was confirmed by the presence of the unique Hpal site, see Figure 6A. The entire mutant polymerase gene was subcloned into pTrc99 as an Ssil-Hindlll fragment as described above in DH10B. The resulting plasmid was designated pTrcTneFY. (Figure 6B). The clone produced active heat stable polymerase.

Example 11: 3'-to-5' Exonuclease and Phe ⁷³⁰ → Tyr ⁷³⁰

Double Mutants

In order to introduce the 3'-* 5' exonuclease mutation and the Phe ⁷³⁰ →Tyr ⁷³⁰ mutation in the same expression vector, pTrc99, it was necessary to first reconstitute both mutations in the pUC-Tne clone. See Figure 7. Both the pUC-Tne (3 '-5 ') and the pUC-TneFY were digested with BamHl. The digested pUC-Tne (3'-A) was dephosphorylated to avoid recirculation in the following ligations. The resulting fragments were purified on a 1% agarose gel. The largest BamHl fragment (4.4 kb) was purified from pUC-Tne (3 '-5') digested DNA and

the smallest BamHl fragment (0.8 kb) containing the Phe ⁷³⁰ →Tyr ⁷³⁰ mutation was purified and ligated to generate pUC-Tne35FY. The proper orientation and the presence of both mutations in the same plasmid was confirmed by Eco47III, Hpal, and Sphl-Hindlll restriction digests. See Figure 7. The entire polymerase containing both mutations was subcloned as a Sytl-

Hindlll fragment in pTrc99 to generate pTrcTne35FY in DH10B. The clone produced active heat stable polymerase.

Example 12: 3' -to- 5' Exonuclease, 5'- to-3' Exonuclease, and Phe ⁷³⁰ →Tyr ⁷³⁰ Triple Mutants

In most of the known polymerases, the 5'-to-3' exonuclease activity is present at the amino terminal region ofthe polymerase (Ollis. D.L., et al, Nature 313, 762-766, 1985; Freemont, P.S., etal, Proteins 1, 66-73. 1986; Joyce, CM., Curr. Opin. Struct. Biol. 1: 123-129 (1991). There are some conserved amino acids that are implicated to be responsible for 5'-to-3' exonuclease activity (Gutman and Minton, Nucl. Acids Res. 21, 4406-4407, 1993). See supra. It is known that 5'-to-3' exonuclease domain is dispensable. The best known example is the Klenow fragment of E. coli Pol I. The Klenow fragment is a natural proteolytic fragment devoid of 5'-to-3' exonuclease activity (Joyce, CM., et al, J. Biol. Chem. 257, 1958-1964, 1990). In order to generate an equivalent mutant for Tne DNA polymerase devoid of 5'-to-3' exonuclease activity, the presence of a unique SphI site present 680 bases from the Sytl site was exploited. pUC-Tne35FY was digested with Hindlll, filled-in with Klenow fragment to generate a blunt-end, and digested with SphI. The 1.9 kb fragment was cloned into an expression vector pTTQ19 (Stark, M.J.R., Gene 51, 255-267, 1987) at the Sphl-Smal sites and was introduced into DH 10B . This cloning strategy generated an in-frame polymerase clone with an initiation codon for methionine from the vector. The resulting clone is devoid of 219 amino terminal amino acids of Tne DNA polymerase. This clone is designated as pTTQTne535FY. The clone

produced active heat stable polymerase. No exonuclease activity could be detected in the mutant polymerase as evidenced by lack of presence of unusual sequence ladders in the sequencing reaction. This particular mutant polymerase is highly suitable for DNA sequencing.

Example 13: 5' -to-3' Exonuclease Deletion and Phe ⁷³⁰ → Tyr ⁷³⁰

Substitution Mutant

In order to generate the 5'-* 3' exonuclease deletion mutant of the Tne DNA polymerase Phe ⁷³⁰ →Tyr ⁷³⁰ mutant, the 1.8 kb Sphl-Spel fragment of pTTQTne535FY was replaced with the identical fragment of pUC-Tne FY. See Fig. 8. A resulting clone, pTTQTne5FY, produced active heat stable DNA polymerase. As measured by the rate of degradation of a labeled primer, this mutant has a modulated, low but detectable, 3 '-5 ' exonuclease activity compared to wild type Tne DNA polymerase. M13/pUC Forward 23-Base Sequencing Primer™, obtainable from LTI, Gaithersburg, MD, was labeled at the 5' end with [P ³² ] ATP and T4 kinase. also obtainable from LTI, Gaithersburg, MD, as described by the manufacturer. The reaction mixtures contained 20 units of either wild-type or mutant Tne DNA polymerase. 0.25 pmol of labeled primer, 20 mM tricine, pH 8.7, 85 mM potassium acetate, 1.2 mM magnesium acetate, and 8% glycerol. Incubation was carried out at 70°C. At various time points, 10 μl aliquots were removed to 5 μl cycle sequencing stop solution and were resolved in a 6 % polyacrylamide sequencing gel followed by andoradiography. While the wild-type polymerase degraded the primer in 5 to 15 minutes, it took the mutant polymerase more than 60 minutes for the same amount of degradation of the primer. Preliminary results suggest that this mutant polymerase is able to amplify more than 12 kb of genomic DNA when used in conjunction with Taq DNA polymerase. Thus, the mutant polymerase is suitable for large fragment PCR.

Example 14: Purification ofthe Mutant Polymerases

The purification of the mutant polymerases was done essentially as described in U.S. Patent Application Serial No. 08/370,190, filed January 9. 1995, entitled "Cloned DNA Polymerases for Thermotoga neapolitana " and as in Example 6, supra, with minor modifications. Specifically, 5 to 10 grams of cells expressing cloned mutant Tne DNA polymerase were lysed by sonication with a Heat Systems Ultrasonic, Inc. Model 375 machine in a sonication buffer comprising 50 mM Tris-HCl (pH 7.4); 8% glycerol; 5 mM 2-mercaptoethanol, 10 mM NaCl, 1 mM EDTA, and 0.5 mM PMSF. The sonication sample was heated at 75 °C for 15 minutes. Following heat treatment, 200 mM NaCl and

0.4% PEI was added to remove nucleic acids. The extract was centrifuged for clarification. Ammonium sulfate was added to 48%, the pellet was resuspended in a column buffer consisting of 25 mM Tris-HCl (pH 7.4); 8% glycerol; 0.5% EDTA; 5 mM 2-mercaptoethanol; 10 mM KCI and loaded on a heparin agarose (LTI) column. The column was washed with 10 column volumes using the loading buffer and eluted with a 10 column volume buffer gradient from 10 mM to 1 M KCI. Fractions containing polymerase activity were pooled and dialyzed in column buffer as above with the pH adjusted to 7.8. The dialyzed pool of fractions were loaded onto a MonoQ (Pharmacia) column. The column was washed and eluted as described above for the heparin column. The active fractions are pooled and a unit assay was performed.

The unit assay reaction mixture contained 25 mM TAPS (pH 9.3), 2 mM MgCl,, 50 mM KCI, 1 mM DTT, 0.2 mM dNTPs, 500 μg/ml DNAse I treated salmon sperm DNA, 21 mCi/ml [αP ³² ] dCTP and various amounts of polymerase in a final volume of 50 μl. After 10 minutes incubation at 70°C, 10 μl of 0.5 M

EDTA was added to the tube. TCA precipitable counts were measured in GF/C filters using 40 μl ofthe reaction mixture.

Example 15: DNA Sequencing with the Mutant Polymerases

M13/pUC 23-base forward sequencing primer was ³² P-end-labeled for use in sequencing by incubating the following mixture at 37 °C for 10 minutes: 60 mM Tris-HCl (pH 7.8), 10 mM MgCl,, 200 mM KCI. 0.2 μM primer, 0.4 μM (2 μCi μl) [γ- ³² P]ATP, 0.2 U/μl T4 polynucleotide kinase. Labeling was terminated by incubating at 55 °C for 5 minutes.

Four 10 μl base-specific sequencing reactions were set up for each test. The polymerase and the ddNTP concentrations were varied as follows:

Test Tne DNA |ddATP| IddCTP] IddGTP] IddTTP] polymerase

1 wild-type 0.4 mM 0.2 mM 0.04 mM 0.4 M

2 TneFY 0.4 mM 0.2 M 0.04 mM 0.4 mM

3 TneFY 0.04 mM 0.02 mM 0.004 mM 0.04 mM

4 TneFY 0.004 mM 0.002 mM 0.0004 mM 0.004 mM

5 Tne35FY 0.4 mM 0.2 mM 0.04 M 0.4 M

6 Tne35FY 0.04 mM 0.02 mM 0.004 mM 0.04 mM

7 Tne35FY 0.004 mM 0.002 M 0.0004 M 0.004 mM

8 Tne535FY 0.4 mM 0.2 mM 0.04 mM 0.4 M

9 Tne535FY 0.04 mM 0.02 mM 0.004 mM 0.04 mM

10 Tne535FY 0.004 mM 0.002 M 0.0004 mM 0.004 mM

Other components ofthe reaction were held constant: 1.1 nM pUC 18 DNA, 22 nM ³² P-end-labeled primer, 30 mM Tris-HCl (pH 9.0), 5 mM MgCl ₂ , 50 mM KCI, 0.05% (w/v) W-l, 0.056 U/μl DNA polymerase (see table), 20 μM dATP, 20 μM dCTP. 20 μM 7-deaza-dGTP, 20 μM dTTP. Samples were incubated in a thermal cycler at 95 °C for 3 minutes, followed by 20 cycles of (30 seconds at 95 °C, 30 seconds at 55 °C, 60 seconds at 70 °C) and 10 cycles of (30 seconds at 95 °C, 60 seconds at 70°C). Reactions were terminated with 5 μl of stop solution (95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue,

0.1%) (w/v) xylene cyanol and denatured for two minutes at 70 °C. Three μl aliquots were separated on a 6% TBE/urea sequencing gel. The dried gel was exposed to BioMAX-MR x-ray film for 16 hours.

Results

Cycle sequencing reactions using P ³² end-labeled primers were prepared using wild-type Tne DNA polymerase and each of the three mutants, TneFY, Tne35FY, and Tne535FY. All four of the polymerases produced sequencing ladders. The TneFY mutant gave only a 9 base sequencing ladder when the Taq cycle sequencing reaction conditions were used. This is suggestive of premature termination due to efficient ddNTP incoφoration. Diluting the dideoxynucleotides by a factor of 100 extended the ladder to about 200 bases. The F-Y mutation in the TneFY polymerase therefore allowed dideoxynucleotides to be incoφorated at a much higher frequency than for wild- type polymerase. The 7«e35FY mutant demonstrated a similar ability to incoφorate dideoxynucleotides. In this case, the sequence extended to beyond

400 bases and the excess P ³² end-labeled M13/pUC forward 23 -Base sequencing primer band remained at the 23-base position in the ladder. The persistence of the 23-base primer band confirmed that the 3' - 5' exonuclease activity had been significantly reduced. The 7«e535FY mutant performed similarly to the 7 2e35FY mutant except that the signal intensity increased by at least fivefold.

The background was very low and relative band intensities were extremely even, showing no patterns of sequence-dependent intensity variation.

Example 16: Generation of 5' -3' exonuclease mutant of full length Tne DNA polymerase

Identification of Two Amino Acids Responsible for 5' -3' Exonuclease Activity

Tne DNA polymerase contains three enzymatic activities similar to E coli

DNA polymerase I: 5 '-3' DNA polymerase activity, 3 '-5' exonuclease activity and 5 '-3' exonuclease activity. This example is directed to the elimination ofthe 5 '-3 ' exonuclease activity in full length Tne DNA polymerase. Gutman and Minton (Nucleic Acids Res. 1993, 27, 4406-4407) identified six (A-F) conserved 5 '-3 ' exonuclease domains containing a total of 10 carboxylates in various DNA polymerases in the poll family. Seven out of 10 carboxylates (in domains A, D and Ε) have been implicated to be involved in divalent metal ions binding as judged from the crystal structure (Kim et al. Nature, 1995, 376, 612-616) of Taq DNA polymerase. However, there was no clear demonstration that these carboxylates are actually involved 5 '-3 'exonuclease activity. In order to find out the biochemical characteristics of some of these carboxylates, two ofthe aspartic acids in domains A and Ε were chosen for mutagenesis. The following aspartic acids in these two domains were identified:

Tne DNA polymerase: 5 F L F D ⁸ G T 10 (domain A) Taq DNA polymerase: 15 L L V D ¹⁸ G H 20

and

Tne DNA polymerase: 132 S L I T G D ¹³⁷ K D M L 141 (domain Ε)

Taq DNA polymerase: 137 R I L T A D ¹⁴² K D L Y 146

2. Isolation of Single Stranded DNA for Mutagenesis

Single stranded DNA was isolated from pSportTne (see infra). pSportTne was introduced into DH5αFTQ (LTI, Gaithersburg, MD) by transformation. A single colony was grown in 2 ml Circle Grow (Bio 101, CA) medium with ampicillin at 37°C for 16 hrs. A 10 ml fresh media was inoculated with 0.1 ml ofthe culture and grown at 37°C until the A590 reached approximately 0.5. At that time, 0.1 ml of M13KO7 helper phage (1X10" pfu/ml, LTI) was added to the culture. The infected culture was grown for 75 min. Kanamycin was then added at 50 μg/ml, and the culture was grown overnight (16 hrs.). The culture was spun down. 9 ml ofthe supernatant was treated with 50 μg each of RNaseA and DNasel in the presence of 10 mM MgCl ₂ for 30 min. at room temperature. To this mixture, 0.25 volume of a cocktail of 3M ammonium acetate plus 20% polyethylene glycol was added and incubated for 20 min. on ice to precipitate phage. The phage was recovered by centrifugation. The phage pellet was dissolved in 200 μl of TE (10 mM Tris-HCl (pH 8) and 1 mM EDTA). The phage solution was extracted twice with equal volume of buffer saturated phenol (LTI, Gaithersburg, MD). twice with equal volume of phenol:chloroform:isoamyl alcohol mixture (25:24:1. LTI. Gaithersburg, MD) and finally, twice with chloroform: isoamyl alcohol (24:1). To the aqueous layer, 0.1 volume of 7.5 M ammonium acetate and 2.5 volume of ethanol were added and incubated for 15 min. at room temperature to precipitate single stranded DNA. The DNA was recovered by centrifugation and suspended in 200 μl TE.

Mutagenesis ofD ⁸ and D ,137

Two oligos were designed to mutagenize D ⁸ and D ¹³⁷ to alanine. The oligos are: 5' GTAGGCCAGGGCTGTGCCGGCAAAGAGAAATAGTC 3'

(SEQ ID NO: 15) (D8A) and 5' GAAGCATATCCTTGGCGCCGGTTAT

TATGAAAATC 3' (SEQ ID NO: 16) (D137A). In the D8A oligo a NgoAIV

(bold underlined) and in the oligo D137A a Kasl (bold underlined) site was created for easy identification of clones following mutagenesis. 200 pmol of each oligo was kinased according to the Muta-gene protocol (Bio-Rad, CA) using 5 units of T4 Kinase (LTI, Gaithersburg, MD). 200 ng of single stranded DNA was annealed with 2 pmol of oligo according to the Muta-gene protocol. The reaction volume was 10 μl. Following the annealing step, complementary DNA synthesis and ligation was canied out using 5 units of wild-type T7 DNA polymerase (USB, Ohio) and 0.5 unit T4 ligase (LTI). 1 μl of the reaction was used to transform a MutS E. coli (obtainable from Dr. Paul Modrich at the Duke University, NC) and selected in agar plates containing ampicillin. A control annealing and synthesis reaction was canied out without addition of any oligo to deteπnine the background. There were 50-60 fold more colonies in the transfomation plates with the oligos than without any oligo. Six colonies from each mutagenic oligo directed synthesis were grown and checked for respective restriction site (NgoAlW or Kasl). For D8A (NgoAΪV), 4 out of 6 generated two fragments (3 kb and 4.1 kb). Since pSportTne has an NgoAlV site near the fl intergenic region, the new NgoAYV site within the Tne DNA polymerase produced the expected fragments. The plasmid was designated as pSportTneNgoAlV. For D137A (Kasl), 5 out of 6 clones produced two expected fragments of 1.1 kb and 6 kb in size. Since pSportTne has another Kasl site, the newly created Kasl site generated these two expected fragments. The plasmid was designated as pSportTneKasI. Both D8A and D137A mutations have been confirmed by DNA sequencing.

4. Reconstruction ofthe Mutant Polymerase into Expression Vector

During the course of expression of Tne DNA polymerase or mutant Tne

DNA polymerase, a variety of clones were constructed. One such clone was designated as pTTQ Tne SeqSl. This plasmid was constructed as follows: first, similar to above mutagenesis technique glycine 195 was changed to an aspartic

acid in pSportTne. A mutation in the coπesponding amino acid in E. coli DNA polymerasel (polA214, domain F) was found to have lost the 5 '-3' exonuclease activity (Gutman and Minton, see above). An Sspl site was created in the mutant polymerase. Second, a 650 bp Sstl-Sphl fragment containing the G195D mutation was subcloned in pUCTne35FY (see infra) to replace the wild type fragment. This plasmid was called pUCTne3022. Finally, the entire mutant Tne DNA polymerase was subcloned from pUCTne3022 into pTTQl 8 as Sstl-Hindlll fragment to generate pTTQTneSeqS 1. To introduce the mutation D8A or D 137A in this expression vector, the 650 bp Sstl-Sphl was replaced with the same Sytl- SphI fragment from pSportTneNgoAlV or pSportTneKasI. The plasmids were designated as pTTQTneNgo(D8A) and pTTQTneKas(D137A), respectively.

5. Confirmation ofthe Mutations by DNA Sequencing

DNA sequencing of both mutant polymerases confirmed the presence of the restriction site NgoATV as well as the mutation D8A; and Kasl site as well as the mutation D137A. Also confirmed by DNA sequencing was the presence ofthe mutation D323A and the Ecø47III restriction site in the 3 '-5 'exonuclease region. In addition, confirmed by DNA sequencing was the F730Y mutation and the Hpal restriction site in the O-helix region of the mutant Tne DNA polymerase.

6. 5' -3 ¹ exonuclease Activity ofthe Mutant Tne DNA Polymerases

The full length mutant DNA polymerase was purified as described above. The 5 '-3 'exonuclease activity was determined as described in the LTI catalog. Briefly. 1 pmol of labeled ( ³² P) Hαelll digested λ DNA (LTI) was used for the assay. The buffer composition is: 25 mM Tris-ΗCl (pΗ 8.3), 5 mM MgCl ₂ , 50 mM NaCl, 0.01% gelatin. The reaction was initiated by the addition of 0, 2, 4,

6 and 10 units of either wild type or mutant Tne DNA polymerase in a 50 μl

reaction. The reaction mix was incubated for 1 hr at 72 °C A IO μl aliquot was subjected to PEI-cellulose thin layer chromatography and the label released was quantitated by liquid scintillation. In this assay, both D8A and D137A mutants showed less than 0.01% label release compared to the wild type Tne DNA polymerase. The result demonstrates that in both D8A and D137A mutants the

5 '-3 'exonuclease activity has been considerably diminished. Thus, it has been confirmed for the first time that these two aspartates are involved with the 5 '-3' exonuclease activity.

7. DNA Sequencing Characteristics ofthe Mutant DNA Polymerases

Four separate base-specific reactions ofthe following composition were set up for each Tne polymerase mutant. 6.5 nM pUC 18, 111 nM M13/pUC 23 base forward sequencing primer, 30 mM Tris-HCl (pH 9.0), 5 mM MgCl ₂ , 10 mM NaCl, 10 mM DTT, 0.05% (w/v) W-l, 0.00185 U/μl inorganic pyrophosphatase, 0.37 μCi/μl (0.37 μM) [α- ³⁵ S]dATP, 16.7 μM α-thio-dATP, 16.7 μM dCTP, 16.7 μM 7-deaza-dGTP, 16.7 μM dTTP, and either 0.042 μM ddATP, 0.3 μM ddCTP, 0.255 μM ddGTP or 0.375 μM ddTTP. In these reactions, the concentrations ofthe various mutants were: 0.185 U/μl Tne535FY, or 0.170 U/μl D8A, or 0.185 U/μl D137A. Reaction volumes were 6 μl each. Sample tubes were incubated in an MJ Research DNA Engine thermal cycler at 95 °C for 3 minutes, followed by 20 cycles of (30 seconds at 95 °C, 30 seconds at 55 °C and 60 seconds at 70°C), and 10 cycles of (30 seconds at 95 °C and 60 seconds at 70 °C). Reactions were terminated with 3 μl of stop solution (95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue, 0.1% (w/v) xylene cyanol) and denatured for two minutes at 70 °C Three μl aliquots were separated on a 6% TBE/urea sequencing gel. The dried gel was exposed to

Kodak BioMAX x-ray film at room temperature approximately 18 hours.

The results of the sequencing data suggest that both D8A and D137A mutants of Tne DNA polymerase produced equivalent sequence ladders with

equal band intensity in all 4 lanes comparable to another Tne DNA polymerase where the 5 '-exonuclease domain was deleted (Tne535FY). This result also suggests that both D8A and D137A mutants are devoid of 5 '-exonuclease activity since no false bands are seen in the sequencing ladder, a characteristic of 5 '-3' exonuclease containing DNA polymerase.

Example 17: Advantages of Tne DNA Polymerase Mutant in

Sequencing Reactions

In this example, the Tne DNA polymerase of Example 12 was used which has the Phe ⁷³⁰ →Tyr ⁷³⁰ mutation (making it non-discriminatory for dNTPs over ddNTPs), the Asp ³²³ →Ala ³²³ mutation (which substantially reduces 3'-to-5' exonuclease activity), and the N-terminal 219 amino acid deletion mutation

(which eliminates 5'-to-3' exonuclease activity).

Sequenase Ver 2.0™ is a modified T7 DNA polymerase sold by Amersham International pic, Little Chalfont, England. Taq DNA polymerase was purchase from LTI, Gaithersburg, MD.

Thermo Sequenase™ is a Taq F→Y mutant containing a 5 '-exonuclease deletion sold by Amersham International pic. Little Chalfont, England.

AmpliTaq FS™ is a Taq F→Y mutant believed to contain a Gly ³⁷ mutation sold by Perkin Elmer ABI, Foster City, CA. Sequitherm™ is a thermophilic DNA polymerase sold by Epicenter,

Madison, WI.

Methods

³⁵ S cycle Sequencing with Tne DNA Polymerase

Four separate base-specific reactions ofthe following composition are set up for each template: 6.5 nM dsDNA, 111 nM primer, 30 mM Tris-HC 1 (pH 9.0),

5 mM MgCl ₂ . 10 mM NaCl, 10 mM DTT, 0.05% (w/v) W-l, 0.185 U/μL Tne DNA polymerase mutant, 0.00185 U/μl thermophilic inorganic pyrophosphatase, 0.37 μCi/μl (0.37 μM) [α- ³⁵ S]dATP, 16.7 μM α-thio-dATP, 16.7 μM dCTP, 16.7 μM 7-deaza-dGTP, 16.7 μM dTTP, and either 0.042 μM ddATP, 0.3 μM ddCTP, 0.255 μM ddGTP or 0.375 μM ddTTP. Reaction volumes are 6 μl each. Sample tubes are incubated in an MJ Research DNA Engine thermal cycler at 95°C for 3 minutes, followed by 20 cycles of (30 seconds at 95°C, 30 seconds at 55°C and 60 seconds at 70°C), and 10 cycles of (30 seconds at 95°C and 60 seconds at 70°C). Reactions are terminated with 3 μl of stop solution (95% (v/v) formamide. 10 mM EDTA (pH 8.0), 0.1 % (w/v) bromophenol blue, 0.1 % (w/v) xylene cyanol) and denatured for 2 minutes at 70°C. Three microliter aliquots are separated on a 6% TBE/urea sequencing gel. The dried gel is exposed to Kodak BioMAX x-ray film at room temperature for approximately 18 hours, unless otherwise specified.

³² P-end Labeled Primer Cycle Sequencing with Tne DNA Polymerase

The sequencing primer is labeled by incubating the following 5 μl reaction for 10 minutes at 37°C: 60 mM Tris-HCl. 10 mM MgCl ₂ , 200 mM KCI. 0.6 μM primer. 0.4 μM (2 μCi/μl) [γ- ³² P]ATP, 0.2 U/μl T4 polynucleotide kinase. The reaction is stopped by incubating 5 minutes at 55 °C. Four separate base-specific reactions of the following composition are then set up for each template: 1.1 nM dsDNA, 67 nM ³² P-end-labeled primer, 30 mM Tris-HCl (pH 9.0), 5 mM MgCl ₂ , 50 mM KCI, 0.05% (w/v) W-l, 0.185 U/μl Tne DNA polymerase, 0.00185 U/μl thermophilic inorganic pyrophosphatase, 20 μM dATP, 20 μM dCTP, 20 μM 7-deaza-dGTP, 20 μM dTTP, and either 0.4 μM ddATP, 0.4 μM ddCTP, 0.4 μM ddGTP or 0.4 μM ddTTP. Reaction volumes are

10 μl each. Sample tubes are incubated in an MJ Research DNA Engine thermal cycler at 95 °C for 3 minutes, followed by 20 cycles of (30 seconds at 95 °C, 30 seconds at 55 °C and 60 seconds at 70 °C), and 10 cycles of (30 seconds at

95 °C and 60 seconds at 70 °C). Reactions are terminated with 5 μl of stop solution (95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue. 0.1% (w/v) xylene cyanol) and denatured for 2 minutes at 70 °C Three μl aliquots are separated on a 6% TBE/urea sequencing gel. The dried gel is exposed to Kodak BioMAX x-ray film at room temperature for approximately 18 hours, unless otherwise specified.

Single-extension Sequencing with Tne DNA Polymerase

This reaction requires either ssDNA or denatured dsDNA. The DNA is annealed to primer in a 10 μl volume by heating for five minutes at 50°C under the following reaction conditions: 150 nM dsDNA and 150 nM primer or 50 nM ssDNA and 50 nM primer with 60 mM Tris-HCl (pH 9.0), 60 mM KCI, 10 mM MgCl ₂ , 0.1% (w/v) W-l. The following labeling reaction is then incubated for five minutes at 50°C in a 15.5 μl volume: 1 Oμl annealed DNA-primer 0.32 μCi/μl (0.32 μM) [α- ³⁵ S]dATP, 48.4 mM Tris HCl (pH 9.0), 48.4 mM KCI, 8.1 mM MgCl ₂ , 194 nM dCTP, 194 nM 7-deaza-dGTP, 194 nM dTTP, 6.5 nM DTT,

0.081% (w/v) W-l. 0.32 U/μl Tne DNA polymerase, 0.0032 U/μl thermophilic inorganic pyrophosphatase. The label mixture is then dispensed into four base- specific reaction tubes. Each tube contains a total reaction volume of 6 μl and is incubated for 5 minutes at 70°C under the following conditions: DNA-labeled primer 0.19 μCi μl (0.19 μM) [α- ³⁵ S]dATP, 28 mM Tris-HCl (pH 9.0), 28 mM

KCI , 4.7 mM MgCl ₂ , 42 μM dATP, 42 μM dCTP, 42 μM 7-deaza-dGTP, 42 μM dTTP, 3.8 mM DTT, 0.047% (w/v) W-l, 0.19 U/μl Tne DNA polymerase, 0.0019 U/μl thermophilic inorganic pyrophosphatase and either 0.83 μM ddATP, 0.83 μM ddCTP, 0.83 μM ddGTP or 0.83 μM ddTTP. Reactions are terminated by adding 4μl of stop solution (95% (v/v) formamide. 10 mM EDTA (pH 8.0), 0.1 %

(w/v) bromophenol blue, 0.1% (w/v) xylene cyanol) and denatured for 2 minutes at 70°C Two μl aliquots are separated on a 6% TBE/urea sequencing gel. The

dried gel is exposed to Kodak BioMAX x-ray film at room temperature for approximately 2 hours, unless otherwise specified.

Fluorescent Dye Primer Sequencing with Tne DNA Polymerase

Four base-specific reactions are set up for each template. The A and C reaction volumes are 5 μl and the G and T reaction volumes are 10 μl. The composition of the reactions are as follows: 20 nM dsDNA or 10 nM ssDNA, with 30 mM Tris-HCl (pH 9.0), 30 mM KCI, 5 mM MgCl ₂ , 0.05% (w/v) W-l, 20 μM dATP, 20 μM dCTP, 20 μM 7-deaza-dGTP, 20 μM dTTP, 0.29 U/μl The DNA polymerase, 0.0029 U/μl thermophilic inorganic pyrophosphatase. Each ofthe four tubes also contains a base-specific dye primer and ddNTP as follows:

A: 0.4 μM JOE dye primer. 0.4 μM ddATP C: 0.4 μM FAM dye primer, 0.4 μM ddCTP G: 0.4 μM TAMRA dye primer, 0.4 μM ddGTP T: 0.4 μM ROX dye primer, 0.4 μM ddTTP Sample tubes are incubated in a thermal cycler at 95°C for 3 minutes, followed by 20 cycles of (30 seconds at 95°C, 30 seconds at 55°C and 60 seconds at 70°C), and 10 cycles of (30 seconds at 95°C and 60 seconds at 70°C). Reactions are pooled, purified over a CentriSep spin column, and dried. The dried pellet is dissolved in 3 μl of 83% formamide, 4.2 mM EDTA (pH 8.0) and heated for 2 minutes at 90°C just before loading the entire sample on a 4.75% polyacrylamide/TBE/urea gel in an ABI 373 Stretch machine. The gel is run at 32 watts for 14 hours.

Fluorescent Dye Terminator Sequencing with Tne DNA Polymerase

One 20 μl reaction is set up for each template. The composition ofthe reaction is an follows: 12.5 nM dsDNA or 6.25 nM ssDNA, with 0.16 μM primer, 30 mM Tris-HCl (pH 9.0), 30 mM KCI, 5 mM MgCl ₂ , 0.05% (w/v) W-

1, 0.6 mM dATP, 0.6 mM dCTP, 1.8 mM dITP, 0.6 mM dTTP, 0.5 U/ml Tne DNA polymerase, 0.005 U/μl thermophilic inorganic pyrophosphatase. The reaction also includes four base-specific dye terminators at a final concentration 16-fold lower than the original concentration supplied by ABI. The sample tube is incubated in a thermal cycler for 25 cycles of (30 seconds at 96°C, 15 seconds at 50°C and 4 minutes at 60°C). The reaction is purified over a CentriSep spin column, and dried. The dried pellet is dissolved in 3 μl of 83% formamide, 4.2 mM EDTA (pH 8.0) and heated for 2 minutes at 90°C just before loading the entire sample on a 4.75% polyacrylamide/TBE/urea gel in an ABI 373 Stretch machine. The gel is run at 32 watts for 14 hours.

Results

Single-extension Sequencing

FIG. 9 shows that the efficient ³⁵ S incoφoration by Tne DNA polymerase mutant provides strong signals in single- and double-strand DNA sequencing. Alkali-denatured pUC 19 DNA ( 1.5 pmol) was sequenced using single-extension sequencing with Tne DNA polymerase of Example 12 as described above (set A); film was exposed for only 2 hours. M 13 mp 19(+) DNA was used at one-tenth the normal amount of template (40 pmol) in the Tne DNA polymerase single- extension sequencing reactions as described (set B); film exposed for 20 hours. Since the Tne mutant produces such a strong signal, templates can be used more economically without sacrificing sequence quality.

FIG. 10 shows that the Tne DNA polymerase mutant generates clear sequence from plasmids containing cDNAs with poly(dA) tails. Alkali-denatured plasmid DNAs containing cDNA inserts (1.5 pmol) were sequenced using either the Tne DNA polymerase mutant in single-extension sequencing (sets A and B) as described, or Sequenase Ver 2.0™ (set C) following the standard kit protocol.

Set A, β-actin cDNA; set B, RPA1 cDNA (a replication protein); and set C, RPA2 cDNA (a replication protein).

FIG. 11 compares the Tne DNA polymerase mutant, Sequenase™ and Taq DNA polymerase generated sequences from a plasmid containing poly(dC). Plasmid DNA (1.5 pmol) containing a poly(dC)-tailed 5' RACE-derived insert was alkali denatured. The DNA was sequenced using Tne DNA polymerase mutant in single-extension sequencing (set A) as described, Sequenase Ver 2.0™ (set B) as described in the kit manual, and by Taq DNA polymerase (set C) following the recommended protocol in the TaqTrack kit (Promega, Madison, WI).

Cycle Sequencing.

FIG. 12 shows that the Tne DNA polymerase mutant in cycle sequencing produces ³⁵ S-labeled sequence 3-fold stronger than Thermo Sequenase™ and without the 60-cycle labeling step. Plasmid DNA (0.5 μg) containing a poly(dC)- tailed 5 ' RACE-derived insert was cycled sequenced using Tne DNA polymerase mutant (set A) as described; film exposure was 6 hours. Using Thermo Sequenase™ as described in the kit manual, the plasmid DNA (0.5 μg) was labeled with ³⁵ S by partial primer extension using an incubation of 60 cycles. This was followed by the standard cycle sequencing protocol in the presence of chain terminators (set B); film exposure was 18 hours. The plasmid DNA (0.5 μg) was cycle sequenced using Taq DNA polymerase (set C) as described in the fmol kit manual; film exposure was 18 hours. Note, uneven band intensities in set C

FIG. 13 shows that the Tne DNA polymerase mutant produces high quality sequences of in vitro amplified DNA. Templates were in vitro amplified directly from E. coli chromosomal DNA, from plasmid pSClOl and from human genomic DNA, purified by simple isopropanol precipitation and quantitated. DNAs (100 fmol) were cycle sequenced as described using the Tne DNA

polymerase mutant and one ofthe amplification primers. Set A, E. coli β poll (~450bp); set B, E. coli nsΕ (-350 bp); set C, ori from pSCl 01 (-1.5 kb); and set D, an exon from human HSINF gene (-750 bp); amplified product sizes in parentheses. Note, these DNAs could not be sequenced using Thermo Sequenase™ because the primers did not meet the extra requirements for the labeling reaction.

FIGS. 14A and 14B show that the Tne DNA polymerase mutant provides superior sequence from double-stranded DNA clones containing poly(dA) or poly(dC) stretches. Fig. 14A, supercoiled plasmid DNAs containing inserts with homopolymers were cycle sequenced using the Tne DNA polymerase mutant as described; film exposure was 6 hours. Set A, RPA1; set B, elf (cap binding protein); and set C, a poly(dC)-tailed 5' RACΕ-derived insert.

FIG. 14B, supercoiled plasmid DNAs containing inserts with homopolymers were cycled sequenced using Taq DNA polymerase (set D) in the fmol kit manual, or SequiTherm™ (sets Ε-G) following the kit manual; film exposure was 18 hours. Set D, RPA; set Ε, RPA; set F, a poly(dC)-tailed 5' RACΕ-derived insert; and set G, elf. Note, the many false stops, especially in the homopolymer region.

FIG. 15 shows cycle sequencing using the Tne DNA polymerase mutant and ³² P end-labeled primer. A sequencing primer was first 5 '-end labeled with

³² P using T4 kinase. A supercoiled plasmid DNA (50 fmol) was cycle sequenced using the Tne DNA polymerase mutant as described; film exposure was 18 hours. The left and right sets are aliquots ofthe same reaction, the right set loaded on the gel 45 minutes after the left.

Fluorescent Automated Sequencing

FIGS. 16A-16C and 16D-16F show a comparison of the Tne DNA polymerase mutant (16A-16C) to AmpliTaq FS™ (16D-16F) in fluorescent dye primer sequencing. pUC19 DNA was sequenced with dye primers (ABI, Foster

City, CA) using either the Tne DNA polymerase mutant or AmpliTaq FS™ as described.

FIGS. 17A-17C and 17D-17F show a comparison of the Tne DNA polymerase mutant (17A-17C) to AmpliTaq FS™ (17D-17F) in fluorescent dye terminator sequencing. pUC 19 DNA was sequenced with dye terminators (ABI,

Foster City, CA) using either the Tne DNA polymerase mutant or AmpliTaq

FS™ as described. Note, greater evenness of peak heights with Tne.

These results demonstrate that the Tne DNA polymerase mutant gives unexpectedly better results in DNA sequencing compared to other DNA polymerases, whether they are similar mutants or not.

From the foregoing description, one skilled in the art can easily ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions without undue experimentation. All patents, patent applications and publications cited herein are incoφorated by reference in their entirety.

SEQϋENCE LISTING

(1) GENERAL INFORMATION:

(i) APPLICANT: Life Technologies, Inc. 8717 Grovemont Circle Gaithersburg, MD 20884-9980 APPLICANTS/INVENTORS: Chatterjee, Deb K.

Hughes, Jr. , A. John

(ii) TITLE OF INVENTION: Cloned DNA Polymerases from Thermotoga and Mutants Thereof

(iii) NUMBER OF SEQUENCES: 22

(iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: STERNE, KESSLER, GOLDSTEIN & FOX P.L.L.C.

(B) STREET: 1100 New York Ave., N.W. , Suite 600

(C) CITY: Washington

(D) STATE: DC

(E) COUNTRY: USA

(F) ZIP: 20005

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy disk

(B) COMPUTER: IBM PC compatible

(C) OPERATING SYSTEM: PC-DOS/MS-DOS

(D) SOFTWARE: Patentin Release #1.0, Version #1.30

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER: To be assigned

(B) FILING DATE: Herewith

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: 08/525,057

(B) FILING DATE: 08-SEP-1995

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: 08/537,397

(B) FILING DATE: 02-OCT-1995

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: 08/537,400

(B) FILING DATE: 02-OCT-1995

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: 08/576,759

(B) FILING DATE: 21-DEC-1995

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: To be assigned

(B) FILING DATE: 14-AUG-1996

(C) CLASSIFICATION:

( iii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Esmond, Robert W.

(B) REGISTRATION NUMBER: 32,893

(C) REFERENCE/DOCKET NUMBER: 0942.280PC03

(ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 202-371-2600

(B) TELEFAX: 202-371-2540

(2) INFORMATION FOR SEQ ID NO:l:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 23 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: GAGCTCACGG GGGATGCAGG AAA 23

(2) INFORMATION FOR SEQ ID NO:2:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2682 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2

ATGGCGAGAC TATTTCTCTT TGATGGCACA GCCCTGGCCT ACAGGGCATA TTACGCCCTC 60

GACAGATCCC TTTCCACATC CACAGGAATT CCAACGAACG CCGTCTATGG CGTTGCCAGG 120

ATGCTCGTTA AATTCATTAA GGAACACATT ATACCCGAAA AGGACTACGC GGCTGTGGCC 180

TTCGACAAGA AGGCAGCGAC GTTCAGACAC AAACTGCTCG TAAGCGACAA GGCGCAAAGG 240

CCAAAGACTC CGGCTCTTCT AGTTCAGCAG CTACCTTACA TCAAGCGGCT GATAGAAGCT 300

CTTGGTTTCA AAGTGCTGGA GCTGGAGGGA TACGAAGCAG ACGATATCAT CGCCACGCTT 360

GCAGTCAGGG CTGCACGTTT TTTGATGAGA TTTTCATTAA TAACCGGTGA CAAGGATATG 420

CTTCAACTTG TAAACGAGAA GATAAAGGTC TGGAGAATCG TCAAGGGGAT ATCGGATCTT 480

GAGCTTTACG ATTCGAAAAA GGTGAAAGAA AGATACGGTG TGGAACCACA TCAGATACCG 540

GATCTTCTAG CACTGACGGG AGACGACATA GACAACATTC CCGGTGTAAC GGGAATAGGT 600

GAAAAGACCG CTGTACAGCT TCTCGGCAAG TATAGAAATC TTGAATACAT TCTGGAGCAT 660

GCCCGTGAAC TCCCCCAGAG AGTGAGAAAG GCTCTCTTGA GAGACAGGGA AGTTGCCATC 720

CTCAGTAAAA AACTTGCAAC TCTGGTGACG AACGCACCTG TTGAAGTGGA CTGGGAAGAG 780

ATGAAATACA GAGGATACGA CAAGAGAAAA CTACTTCCGA TATTGAAAGA ACTGGAGTTT 840

GCTTCCATCA TGAAGGAACT TCAACTGTAC GAAGAAGCAG AACCCACCGG ATACGAAATC 900

GTGAAGGATC ATAAGACCTT CGAAGATCTC ATCGAAAAGC TGAAGGAGGT TCCATCTTTT 960

GCCCTGGACC TTGAAACGTC CTCCTTGGAC CCGTTCAACT GTGAGATAGT CGGCATCTCC 1020

GTGTCGTTCA AACCGAAAAC AGCTTATTAC ATTCCACTTC ATCACAGAAA CGCCCACAAT 1080

CTTGATGAAA CACTGGTGCT GTCGAAGTTG AAAGAGATCC TCGAAGACCC GTCTTCGAAG 1140

ATTGTGGGTC AGAACCTGAA GTACGACTAC AAGGTTCTTA TGGTAAAGGG TATATCGCCA 1200

GTTTATCCGC ATTTTGACAC GATGATAGCT GCATATTTGC TGGAGCCAAA CGAGAAAAAA 1260

TTCAATCTCG AAGATCTGTC TTTGAAATTT CTCGGATACA AAATGACGTC TTATCAGGAA 1320

CTGATGTCGT TTTCCTCACC ACTTTTTGGT TTCAGCTTTG CGGATGTTCC GGTAGACAAG 1380

GCTGCCGAAT ACTCCTGCGA GGATGCAGAC ATCACTTATA GGCTCTACAA GATACTCAGC 1440

ATGAAGCTCC ATGAAGCGGA ACTTGAGAAC GTCTTCTACA GGATAGAGAT GCCGTTGGTG 1500

AACGTCTTGG CACGAATGGA ATTCAACTGG GTGTATGTTG ACACAGAATT CCTGAAAAAG 1560

CTCTCGGAGG AGTACGGCAA AAAGCTCGAG GAACTGGCCG AAAAAATCTA CCAGATAGCA 1620

GGTGAGCCCT TCAACATCAA TTCTCCAAAA CAGGTTTCAA ACATCCTTTT TGAGAAGCTG 1680

GGAATAAAAC CCCGTGGAAA AACGACAAAA ACAGGAGATT ACTCTACCAG GATAGAGGTG 1740

TTGGAAGAGA TAGCGAATGA GCACGAGATA GTACCCCTCA TTCTCGAGTT CAGAAAGATC 1800

CTGAAACTGA AATCGACCTA CATAGACACC CTTCCGAAAC TTGTGAACCC GAAAACCGGA 1860

AGATTTCATG CATCTTTCCA CCAGACGGGT ACCGCCACTG GCAGGTTGAG TAGCAGTGAT 1920

CCAAATCTTC AGAATCTTCC GACAAAGAGC GAAGAGGGAA AAGAAATTAG AAAAGCGATT 1980

GTGCCCCAGG ATCCAGACTG GTGGATCGTC AGTGCGGATT ATTCCCAAAT AGAACTCAGA 2040

ATCCTCGCTC ATCTCAGTGG TGATGAGAAC CTTGTGAAGG CCTTCGAGGA GGGCATCGAT 2100

GTGCACACCT TGACTGCCTC CAGGATCTAC AACGTAAAGC CAGAAGAAGT GAACGAAGAA 2160

ATGCGACGGG TTGGAAAGAT GGTGAACTTC TCTATAATAT ACGGTGTCAC ACCGTACGGT 2220

CTTTCTGTGA GACTTGGAAT ACCGGTTAAA GAAGCAGAAA AGATGATTAT CAGCTATTTC 2280

ACACTGTATC CAAAGGTGCG AAGCTACATC CAGCAGGTTG TTGCAGAGGC AAAAGAGAAG 2340

GGCTACGTCA GGACTCTCTT TGGAAGAAAA AGAGATATTC CCCAGCTCAT GGCAAGGGAC 2400

AAGAACACCC AGTCCGAAGG CGAAAGAATC GCGATAAACA CCCCCATTCA GGGAACTGCG 2460

GCAGATATAA TAAAATTGGC TATGATAGAT ATAGACGAGG AGCTGAGAAA AAGAAACATG 2520

AAATCCAGAA TGATCATTCA GGTTCATGAC GAACTGGTCT TCGAGGTTCC CGATGAGGAA 2580

AAAGAAGAAC TAGTTGATCT GGTGAAGAAC AAAATGACAA ATGTGGTGAA ACTCTCTGTG 2640

CCTCTTGAGG TTGACA AG CATCGGAAAA AGCTGGTCTT GA 2682 (2) INFORMATION FOR SEQ ID NO:3:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 893 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:

Met Ala Arg Leu Phe Leu Phe Asp Gly Thr Ala Leu Ala Tyr Arg Ala 1 5 10 15

Tyr Tyr Ala Leu Asp Arg Ser Leu Ser Thr Ser Thr Gly lie Pro Thr 20 25 30

Asn Ala Val Tyr Gly Val Ala Arg Met Leu Val Lys Phe lie Lys Glu 35 40 45

His lie lie Pro Glu Lys Asp Tyr Ala Ala Val Ala Phe Asp Lys Lys 50 55 60

Ala Ala Thr Phe Arg His Lys Leu Leu Val Ser Asp Lys Ala Gin Arg 65 70 75 80

Pro Lys Thr Pro Ala Leu Leu Val Gin Gin Leu Pro Tyr lie Lys Arg 85 90 95

Leu lie Glu Ala Leu Gly Phe Lys Val Leu Glu Leu Glu Gly Tyr Glu 100 105 110

Ala Asp Asp lie lie Ala Thr Leu Ala Val Arg Ala Ala Arg Phe Leu 115 120 125

Met Arg Phe Ser Leu lie Thr Gly Asp Lys Asp Met Leu Gin Leu Val 130 135 140

Asn Glu Lys lie Lys Val Trp Arg lie Val Lys Gly lie Ser Asp Leu 145 150 155 160

Glu Leu Tyr Asp Ser Lys Lys Val Lys Glu Arg Tyr Gly Val Glu Pro 165 170 175

His Gin lie Pro Asp Leu Leu Ala Leu Thr Gly Asp Asp lie Asp Asn 180 185 190 lie Pro Gly Val Thr Gly lie Gly Glu Lys Thr Ala Val Gin Leu Leu 195 200 205

Gly Lys Tyr Arg Asn Leu Glu Tyr lie Leu Glu His Ala Arg Glu Leu 210 215 220

Pro Gin Arg Val Arg Lys Ala Leu Leu Arg Asp Arg Glu Val Ala lie 225 230 235 240

Leu Ser Lys Lys Leu Ala Thr Leu Val Thr Asn Ala Pro Val Glu Val 245 250 255

Asp Trp Glu Glu Met Lys Tyr Arg Gly Tyr Asp Lys Arg Lys Leu Leu 260 265 270

Pro lie Leu Lys Glu Leu Glu Phe Ala Ser lie Met Lys Glu Leu Gin 275 280 285

Leu Tyr Glu Glu Ala Glu Pro Thr Gly Tyr Glu lie Val Lys Asp His 290 295 300

Lys Thr Phe Glu Asp Leu lie Glu Lys Leu Lys Glu Val Pro Ser Phe 305 310 315 320

Ala Leu Asp Leu Glu Thr Ser Ser Leu Asp Pro Phe Asn Cys Glu lie 325 330 335

Val Gly lie Ser Val Ser Phe Lys Pro Lys Thr Ala Tyr Tyr lie Pro 340 345 350

Leu His His Arg Asn Ala His Asn Leu Asp Glu Thr Leu Val Leu Ser 355 360 365

Lys Leu Lys Glu He Leu Glu Asp Pro Ser Ser Lys He Val Gly Gin 370 375 380

Asn Leu Lys Tyr Asp Tyr Lys Val Leu Met Val Lys Gly He Ser Pro 385 390 395 400

Val Tyr Pro His Phe Asp Thr Met He Ala Ala Tyr Leu Leu Glu Pro 405 410 415

Asn Glu Lys Lys Phe Asn Leu Glu Asp Leu Ser Leu Lys Phe Leu Gly 420 425 430

Tyr Lys Met Thr Ser Tyr Gin Glu Leu Met Ser Phe Ser Ser Pro Leu 435 440 445

Phe Gly Phe Ser Phe Ala Asp Val Pro Val Asp Lys Ala Ala Glu Tyr 450 455 460

Ser Cys Glu Asp Ala Asp He Thr Tyr Arg Leu Tyr Lys He Leu Ser 465 470 475 480

Met Lys Leu His Glu Ala Glu Leu Glu Asn Val Phe Tyr Arg He Glu 485 490 495

Met Pro Leu Val Asn Val Leu Ala Arg Met Glu Phe Asn Trp Val Tyr 500 505 510

Val Asp Thr Glu Phe Leu Lys Lys Leu Ser Glu Glu Tyr Gly Lys Lys 515 520 525

Leu Glu Glu Leu Ala Glu Lys He Tyr Gin He Ala Gly Glu Pro Phe 530 535 540

Asn He Asn Ser Pro Lys Gin Val Ser Asn He Leu Phe Glu Lys Leu 545 550 555 560

Gly He Lys Pro Arg Gly Lys Thr Thr Lys Thr Gly Asp Tyr Ser Thr 565 570 575

Arg He Glu Val Leu Glu Glu He Ala Asn Glu His Glu He Val Pro 580 585 590

Leu He Leu Glu Phe Arg Lys He Leu Lys Leu Lys Ser Thr Tyr He 595 600 605

Asp Thr Leu Pro Lys Leu Val Asn Pro Lys Thr Gly Arg Phe His Ala 610 615 620

Ser Phe His Gin Thr Gly Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 625 630 635 640

Pro Asn Leu Gin Asn Leu Pro Thr Lys Ser Glu Glu Gly Lys Glu He 645 650 655

Arg Lys Ala He Val Pro Gin Asp Pro Asp Trp Trp He Val Ser Ala 660 665 670

Asp Tyr Ser Gin He Glu Leu Arg He Leu Ala His Leu Ser Gly Asp 675 680 685

Glu Asn Leu Val Lys Ala Phe Glu Glu Gly He Asp Val His Thr Leu 690 695 700

Thr Ala Ser Arg He Tyr Asn Val Lys Pro Glu Glu Val Asn Glu Glu 705 710 715 720

Met Arg Arg Val Gly Lys Met Val Asn Phe Ser He He Tyr Gly Val 725 730 735

Thr Pro Tyr Gly Leu Ser Val Arg Leu Gly He Pro Val Lys Glu Ala 740 745 750

Glu Lys Met He He Ser Tyr Phe Thr Leu Tyr Pro Lys Val Arg Ser 755 760 765

Tyr He Gin Gin Val Val Ala Glu Ala Lys Glu Lys Gly Tyr Val Arg 770 775 780

Thr Leu Phe Gly Arg Lys Arg Asp He Pro Gin Leu Met Ala Arg Asp 785 790 795 800

Lys Asn Thr Gin Ser Glu Gly Glu Arg He Ala He Asn Thr Pro He 805 810 815

Gin Gly Thr Ala Ala Asp He He Lys Leu Ala Met He Asp He Asp 820 825 830

Glu Glu Leu Arg Lys Arg Asn Met Lys Ser Arg Met He He Gin Val 835 840 845

His Asp Glu Leu Val Phe Glu Val Pro Asp Glu Glu Lys Glu Glu Leu 850 855 860

Val Asp Leu Val Lys Asn Lys Met Thr Asn Val Val Lys Leu Ser Val 865 870 875 880

Pro Leu Glu Val Asp He Ser He Gly Lys Ser Trp Ser 885 890

(2) INFORMATION FOR SEQ ID NO:4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4 :

Pro Ser Phe Ala Leu Asp Leu Glu Thr Ser Ser 1 5 10

(2) INFORMATION FOR SEQ ID NO:5:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:

Pro Val Phe Ala Phe Asp Thr Glu Thr Asp Ser 1 5 10

(2) INFORMATION FOR SEQ ID NO:6:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: ll amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:6

Gly Pro Val Ala Phe Asp Ser Glu Thr Ser Ala 1 5 10

(2) INFORMATION FOR SEQ ID NO:7:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7 :

Met He Val Ser Asp He Glu Ala Asn Ala 1 5 10

(2) INFORMATION FOR SEQ ID NO:8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 26 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: GACGTTTCAA GCGCTAGGGC AAAAGA 26

(2) INFORMATION FOR SEQ ID NO:9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: Lys Met Val Asn Phe Ser He He Tyr Gly

1 5 10

(2) INFORMATION FOR SEQ ID NO:10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:

Lys Ala He Asn Phe Gly Leu He Tyr Gly 1 5 10

(2) INFORMATION FOR SEQ ID NO:11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:

Lys Ala He Thr Phe Gly He Leu Tyr Gly 1 5 10

(2) INFORMATION FOR SEQ ID NO:12:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: Lys Thr Phe He Tyr Gly Phe Leu Tyr Gly

10

(2) INFORMATION FOR SEQ ID NO:13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:

Lys Thr He Asn Phe Gly Val Leu Tyr Gly 1 5 10

(2) INFORMATION FOR SEQ ID NO:14:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 31 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: CDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: GTATATTATA GAGTAGTTAA CCATCTTTCC A 31 (2) INFORMATION FOR SEQ ID NO:15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 36 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: CDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:15 GTAGGCCAGG GGCTGTGCCG GCAAAGAGAA ATAGTC 36

( 2 ) INFORMATION FOR SEQ ID NO : 16 :

( i ) SEQUENCE CHARACTERISTICS :

(A) LENGTH : 35 base pairs

(B) TYPE : nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: cDNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: GAAGGATATC CTTGGCGCCG GTTATTATGA AAATC 35

(2) INFORMATION FOR SEQ ID NO:17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1310 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: linear

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:

ATGGCGAGAC TATTTCTCTT TGATGGCACA GCCCTGGCCT ACAGGGCATA TTACGCCCTC 60

GACAGATCCC TTTCCACATC CACAGGAATT CCAACGAACG CCGTCTATGG CGTTGCCAGG 120

ATGCTCGTTA AATTCATTAA GGAACACATT ATACCCGAAA AGGACTACGC GGCTGTGGCC 180

TTCGACAAGA AGGCAGCGAC GTTCAGACAC AAACTGCTCG TAAGCGACAA GGCGCAAAGG 240

CCAAAGACGC CGGCTCTTCT AGTTCAGCAG CTACCTTACA TCAAGCGGCT GATAGAAGCT 300

CTTGGTTTCA AAGTGCTGGA GCTGGAAGGG TACGAAGCAG ACGATATCAT CGCCACGCTT 360

GCAGCAAAGG GCTGCACGTT TTTTGATGAG ATTTTCATAA TAACCGGTGA CAAGGATATG 420

CTTCAACTTG TAAACGAGAA GATAAAGGTC TGGAGAATCG TCAAGGGGAT ATCGGATCTT 480

GAGCTTTACG ATTCGAAAAA GGTGAAAGAA AGATACGGTG TGGAACCACA TCAGATACCG 540

GATCTTCTAG CACTGACGGG AGACGACATA GACAACATTC CCGGTGTAAC GGGAATAGGT 600

GAAAAGACCG CTGTACAGCT TCTCGGCAAG TATAGAAATC TTGAATACAT TCTGGAGCAT 660

GCCCGTGAAC TCCCCCAGAG AGTGAGAAAG GCTCTCTTGA GAGACAGGGA AGTTGCCATC 720

CTCAGTAAAA AACTTGCAAC TCTGGTGACG AACGCACCTG TTGAAGTGGA CTGGGAAGAG 780

ATGAAATACA GAGGATACGA CAAGAGAAAA CTACTTCCGA TATTGAAAGA ACTGGAGTTT 840

GCTTCCATCA TGAAGGAACT TCAACTGTAC GAAGAAGCAG AACCCACCGG ATACGAAATC 900

GTGAAGGATC ATAAGACCTT CGAAGATCTC ATCGAAAAGC TGAAGGAGGT TCCATCTTTT 960

GCCCTGGACC TTGAAACGTC CTCCTTGGAC CCGTTCAACT GTGAGATAGT CGGCATCTCC 1020

GTGTCGTTCA AACCGAAAAC AGCTTATTAC ATTCCACTTC ATCACAGAAA CGCCCACAAT 1080

CTTGATGAAA CACTGGTGCT GTCGAAGTTG AAAGAGATCC TCGAAGACCC GTCTTCGAAG 1140

ATTGTGGGTC AGAACCTGAA GTACGACTAC AAGGTTCTTA TGGTAAAGGG TATATCGCCA 1200

GTTTATCCGC ATTTTGACAC GATGATAGCT GCATATTTGC TGGAGCCAAA CGAGAAAAAA 1260

TTCAATCTCG AAGATCTGTC TTTGAAATTT CTCGGATACA AAATGACGTC 1310

(2) INFORMATION FOR SEQ ID NO:18:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 436 amino acids

(B) TYPE: amino acid (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:

Met Ala Arg Leu Phe Leu Phe Asp Gly Thr Ala Leu Ala Tyr Arg Ala 1 5 10 15

Tyr Tyr Ala Leu Asp Arg Ser Leu Ser Thr Ser Thr Gly He Pro Thr 20 25 30

Asn Ala Val Tyr Gly Val Ala Arg Met Leu Val Lys Phe He Lys Glu 35 40 45

His He He Pro Glu Lys Asp Tyr Ala Ala Val Ala Phe Asp Lys Lys 50 55 60

Ala Ala Thr Phe Arg His Lys Leu Leu Val Ser Asp Lys Ala Gin Arg 65 70 75 80

Pro Lys Thr Pro Ala Leu Leu Val Gin Gin Leu Pro Tyr He Lys Arg 85 90 95

Leu He Glu Ala Leu Gly Phe Lys Val Leu Glu Leu Glu Gly Tyr Glu 100 105 110

Ala Asp Asp He He Ala Thr Leu Ala Ala Lys Gly Cys Thr Phe Phe 115 120 125

Asp Glu He Phe He He Thr Gly Asp Lys Asp Met Leu Gin Leu Val 130 135 140

Asn Glu Lys He Lys Val Trp Arg He Val Lys Gly He Ser Asp Leu 145 150 155 160

Glu Leu Tyr Asp Ser Lys Lys Val Lys Glu Arg Tyr Gly Val Glu Pro 165 170 175

His Gin He Pro Asp Leu Leu Ala Leu Thr Gly Asp Asp He Asp Asn 180 185 190

He Pro Gly Val Thr Gly He Gly Glu Lys Thr Ala Val Gin Leu Leu 195 200 205

Gly Lys Tyr Arg Asn Leu Glu Tyr He Leu Glu His Ala Arg Glu Leu 210 215 220

Pro Gin Arg Val Arg Lys Ala Leu Leu Arg Asp Arg Glu Val Ala He 225 230 235 240

Leu Ser Lys Lys Leu Ala Thr Leu Val Thr Asn Ala Pro Val Glu Val 245 250 255

Asp Trp Glu Glu Met Lys Tyr Arg Gly Tyr Asp Lys Arg Lys Leu Leu 260 265 270

Pro He Leu Lys Glu Leu Glu Phe Ala Ser He Met Lys Glu Leu Gin 275 280 285

Leu Tyr Glu Glu Ala Glu Pro Thr Gly Tyr Glu He Val Lys Asp His 290 295 300

Lys Thr Phe Glu Asp Leu He Glu Lys Leu Lys Glu Val Pro Ser Phe 305 310 315 320

Ala Leu Asp Leu Glu Thr Ser Ser Leu Asp Pro Phe Asn Cys Glu He 325 330 335

Val Gly He Ser Val Ser Phe Lys Pro Lys Thr Ala Tyr Tyr He Pro 340 345 350

Leu His His Arg Asn Ala His Asn Leu Asp Glu Thr Leu Val Leu Ser 355 360 365

Lys Leu Lys Glu He Leu Glu Asp Pro Ser Ser Lys He Val Gly Gin 370 375 380

Asn Leu Lys Tyr Asp Tyr Lys Val Leu Met Val Lys Gly He Ser Pro 385 390 395 400

Val Tyr Pro His Phe Asp Thr Met He Ala Ala Tyr Leu Leu Glu Pro 405 410 415

Asn Glu Lys Lys Phe Asn Leu Glu Asp Leu Ser Leu Lys Phe Leu Gly 420 425 430

Tyr Lys Met Thr 435

(2) INFORMATION FOR SEQ ID NO:19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 687 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: DNA (genomic)

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:

TCGTACCNGG GNTCNCNANA TCGACTGCAG CATGCAAGCT GGCTAATCAT GGTCATAGCT 60

GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 120

AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 180

ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 240

CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 300

GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 360

ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 420

CAGGAACCGT TAAAAAGGCC GCGTTGCTGG GCGTTTTTCC ATAGGCTCCG CCCCCCTTGA 480

CGAGCATCAC AAAAATTCGA CGCTTCAAGT TCAGAGGTGG GCGAAACCCG ACAGGGACTA 540

TAAAGATTAC CAGGGCGTTT TCCCCCTGGG AAGCTNCCTT CGTGCGCTCT CCTGTTCCCG 600

AACCTGGCCG GTTTAACCGG GATACCNGNT CGGCCTTTTN TCCCCTTNGG GGGAANCCTT 660

GGGGNTTTTN GNAAAANGCT AAGGGTT 687 (2) INFORMATION FOR SEQ ID NO:20:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 701 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: DNA (genomic)

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:

GCTCGTACCG GGGATCTNNN ANATCGACTG CAGCATGCAA GCTTGGCGTA ATCATGGTCA 60

TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 120

AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG 180

CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 240

CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC 300

TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 360

CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 420

AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGGCGTTT TTTCCATAGG CTCCGCCCCC 480

CTGANGAGCA TCANAAAAAT CGANGCTCAN GTCANAGGTG GCGAAACCCG ACAGGNCTAT 540

TAAAAGATNC CCAGGCGTTT TCCCCCCTGG GAAGCTCCCT CGTGGGGCTC TCCTGGTTNC 600

GGNNCCCTGN CCGGNTTACC GGGGATAANC TTGTTCCGGN CTTTNTCCCC TTCNGGGAAA 660

ANGGTGGGGG GTTTTNTNNA AAAGGCTCAA AGGCTGGTAN G 701 (2) INFORMATION FOR SEQ ID NO:21:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 717 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: DNA (genomic)

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: GNTNTAGNNN GGNCTAANNG GCGGGGAAAT CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT 60

CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 120

GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 180

GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 240

CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 300

TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 360

TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAAATCAGGG 420

GATAACGCAG GGAAAGAACA TGTGAGCAAA AGGCCCAGCA AAAGGCCAGG AACCCGTAAA 480

AAGGCCGCGT TGCCTGGCGT TTTTCCATAG GCTCCGCCCC CCTTGACGAG CAATCACAAA 540

AATCGACGCT CAAAGTCAAG AGGTGGCGAA ACCCCGACAG GGACTTATAA AGATACCCAG 600

GCCGTTTCCC CCTGGAAGCT CCCCTCCGTG CGCTTCTCCT TGGTTCCCGA CCCTGCCGCT 660

TTACCNGGAT NCCTGTCCGC CCTTTTNTCC CTTTCNGGNA ACCGGGCGCT TTTTTTT 717

(2) INFORMATION FOR SEQ ID NO:22:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 713 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: both

(D) TOPOLOGY: both

(ii) MOLECULE TYPE: DNA (genomic)

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:

NNNNCNNNNG GCTGANAGCG ATAAATCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC 60

CTGCAGGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 120

TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 180

CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 240

AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 300

TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 360

GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 420

CGCAGGAAAG AACATGTTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 480

CGTTTGCTGG CGTTTTTCCC ATAGGCTCCG CCCCCCTTGA CGAACCATCA CAAAAATCGA 540

CGCTCAATTC AGAAGTTGGC GAAAACCCGA CAGGACTAAT AAAGATACCC AGCGTTTCCC 600

CCCCTGGAAA CTCCCCTCCG TTGCGCCTCT CCCTGTTCCC GAACCTTGCC CGCTTACCGG 660

GAATACCTTG TCCNCCTTTT CTCCCCTTCC GGGAANCGTT NGCGCCTTTC CCC 713