Title:
CLONING METHODS AND OTHER METHODS OF PRODUCING CELLS
Document Type and Number:
WIPO Patent Application WO2003057863
Kind Code:
A3
Abstract:
The present invention relates to a method of producing a viable hybrid cell having a single functional mitochondrial population. The method comprises the step of introducing genomic DNA from a mitochondrially depleted donor cell into a recipient cell from which genomic DNA has been removed.
Inventors:
ST JOHN JUSTIN (GB)
CAMPBELL KEITH HENRY STOCKMAN (GB)
CAMPBELL KEITH HENRY STOCKMAN (GB)
Application Number:
PCT/GB2003/000095
Publication Date:
October 16, 2003
Filing Date:
January 14, 2003
Export Citation:
Assignee:
UNIV BIRMINGHAM (GB)
ST JOHN JUSTIN (GB)
CAMPBELL KEITH HENRY STOCKMAN (GB)
ST JOHN JUSTIN (GB)
CAMPBELL KEITH HENRY STOCKMAN (GB)
International Classes:
C12N15/873; (IPC1-7): A01K67/027
Domestic Patent References:
| WO2001030980A2 | 2001-05-03 |
Other References:
JUN ALBERT S ET AL: "Use of transmitochondrial cybrids to assign a complex I defect to the mitochondrial DNA-encoded NADH dehydrogenase subunit 6 gene mutation at nucleotide pair 14459 that causes Leber hereditary optic neuropathy and dystonia.", MOLECULAR AND CELLULAR BIOLOGY, vol. 16, no. 3, 1996, pages 771 - 777, XP002245808, ISSN: 0270-7306
CAMPBELL K H S ET AL: "NUCLEAR TRANSFER IN PRACTICE", CLONING AND STEM CELLS, MARY ANN LIEBERT, LARCHMONT, US, vol. 3, no. 4, 2001, pages 201 - 208, XP008009809, ISSN: 1536-2302
SHITARA H ET AL: "Selective and continuous elimination of mitochondria microinjected into mouse eggs from sprematids, but not from liver cells, occurs throughout embryogenesis", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 156, November 2000 (2000-11-01), pages 1277 - 1284, XP002955772, ISSN: 0016-6731
BLERKOM VAN J ET AL: "MITOCHONDRIAL TRANSFER BETWEEN OOCYTES: POTENTIAL APPLICATIONS OF MITOCHONDRIAL DONATION AND THE ISSUE OF HETEROPLASMY", HUMAN REPRODUCTION, IRL PRESS, OXFORD, GB, vol. 13, no. 10, 1998, pages 2857 - 2868, XP001002313, ISSN: 0268-1161
CAMPBELL K H S ET AL: "NUCLEAR TRANSFER IN PRACTICE", CLONING AND STEM CELLS, MARY ANN LIEBERT, LARCHMONT, US, vol. 3, no. 4, 2001, pages 201 - 208, XP008009809, ISSN: 1536-2302
SHITARA H ET AL: "Selective and continuous elimination of mitochondria microinjected into mouse eggs from sprematids, but not from liver cells, occurs throughout embryogenesis", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 156, November 2000 (2000-11-01), pages 1277 - 1284, XP002955772, ISSN: 0016-6731
BLERKOM VAN J ET AL: "MITOCHONDRIAL TRANSFER BETWEEN OOCYTES: POTENTIAL APPLICATIONS OF MITOCHONDRIAL DONATION AND THE ISSUE OF HETEROPLASMY", HUMAN REPRODUCTION, IRL PRESS, OXFORD, GB, vol. 13, no. 10, 1998, pages 2857 - 2868, XP001002313, ISSN: 0268-1161
Download PDF: