Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
CLONING VECTOR
Document Type and Number:
WIPO Patent Application WO/2017/002733
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: an expression vector capable of expressing, with higher expression efficiency, a protein derived from an exogenous structural gene through genetic engineering by using a yeast belonging to the genus Schizosaccharomyces as a host; a cloning vector for creating the expression vector; a method for manufacturing the expression vector; a transformant having an expression cassette of the expression vector; a method for manufacturing the transformant; and a protein production method using the transformant. This cloning vector is characterized by having a promoter that can function in a yeast belonging to the genus Schizosaccharomyces, a cloning site located downstream of the promoter and for introducing an exogenous structural gene to be controlled by the promoter, and a terminator included in the ihc2 gene of the yeast belonging to the genus Schizosaccharomyces. The expression vector is characterized by having the promoter, the exogenous structural gene, and the terminator included in the ihc2 gene.

Inventors:
TANAKA, Takayuki (Limited 5-1, Marunouchi 1-chome, Chiyoda-k, Tokyo 05, 〒1008405, JP)
KOTANI, Tetsuya (Limited 5-1, Marunouchi 1-chome, Chiyoda-k, Tokyo 05, 〒1008405, JP)
HARA, Futoshi (Limited 5-1, Marunouchi 1-chome, Chiyoda-k, Tokyo 05, 〒1008405, JP)
Application Number:
JP2016/068866
Publication Date:
January 05, 2017
Filing Date:
June 24, 2016
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
ASAHI GLASS COMPANY, LIMITED (5-1 Marunouchi 1-chome, Chiyoda-ku Tokyo, 05, 〒1008405, JP)
International Classes:
C12N15/09; C12N1/19; C12P21/00
Domestic Patent References:
WO2014030644A12014-02-27
Other References:
YAMANISHI M. ET AL.: "A Genome-Wide Activity Assessment of Terminator Regions in Saccharomyces cerevisiae Provides a ''Terminatome'' Toolbox.", ACS SYNTHETIC BIOLOGY, vol. 2, 11 February 2013 (2013-02-11), pages 337 - 347, XP055342770, ISSN: 2161-5063
DATABASE GenBank 27 February 2015 (2015-02-27), "Schizosaccharomyces pombe chromosome I, complete sequence", XP055192792, Database accession no. CU 329670.1
See also references of EP 3318631A4
Attorney, Agent or Firm:
SENMYO, Kenji et al. (4th Floor, SIA Kanda Square 17, Kanda-konyacho, Chiyoda-k, Tokyo 35, 〒1010035, JP)
Download PDF: