Title:
COLD STORAGE SOLUTION AND STORAGE METHOD FOR MOUSE SPERM
Document Type and Number:
WIPO Patent Application WO/2018/101270
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide, with respect to the cold storage of mouse sperm, a cold storage solution and a storage method that enable cold storage for a longer period of time than the prior art. The present invention provides a cold storage solution for the cold storage of the mouse sperm held in cauda epididymis, wherein the storage solution contains dimethyl sulfoxide or dimethyl sulfoxide and quercitin. The present invention also provides a method for the cold storage of mouse sperm using this storage solution. Mouse sperm that has been cold-stored in accordance with the present invention can maintain a high fertility and motility.
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Inventors:
NAKAGATA NAOMI (JP)
TAKEO TORU (JP)
YOSHIMOTO HIDETAKA (JP)
TAKEO TORU (JP)
YOSHIMOTO HIDETAKA (JP)
Application Number:
PCT/JP2017/042653
Publication Date:
June 07, 2018
Filing Date:
November 28, 2017
Export Citation:
Assignee:
UNIV KUMAMOTO NAT UNIV CORP (JP)
International Classes:
A01N1/00; C12N1/04; C12N5/076
Domestic Patent References:
| WO1998037182A1 | 1998-08-27 | |||
| WO2014162910A1 | 2014-10-09 |
Other References:
TAKEO, TORU ET AL.: "Establishment of a transport system for mouse epididymal sperm at refrigerated temperatures", CRYOBIOLOGY, vol. 65, no. 3, 2 June 2012 (2012-06-02), pages 163 - 168, XP055489424
JONES, R. C.: "THE USE OF DIMETHYL SULPHOXIDE, GLYCEROL, AND RECONSTITUTED SKIM MILK FOR THEPRESERVATION OF RAM SPERMATOZOA", AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, vol. 18, no. 4, 1965, pages 877 - 885, XP055489430
KHALIFA, T. A. A. ET AL.: "Testing Usability of Butylated Hydroxytoluene in Conservation of Goat Semen", REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, no. 5, 2008, pages 525 - 530, XP055489437
JOHINKE, D. ET AL.: "Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa", ANIMAL REPRODUCTION SCIENCE, vol. 151, no. 3-4, 2014, pages 208 - 219, XP055489444
YOSHIMOTO, HIDETAKA ET AL., BIOLOGY OF REPRODUCTION, vol. 97, no. 6, 2017, pages 883 - 891
JONES, R. C.: "THE USE OF DIMETHYL SULPHOXIDE, GLYCEROL, AND RECONSTITUTED SKIM MILK FOR THEPRESERVATION OF RAM SPERMATOZOA", AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, vol. 18, no. 4, 1965, pages 877 - 885, XP055489430
KHALIFA, T. A. A. ET AL.: "Testing Usability of Butylated Hydroxytoluene in Conservation of Goat Semen", REPRODUCTION IN DOMESTIC ANIMALS, vol. 43, no. 5, 2008, pages 525 - 530, XP055489437
JOHINKE, D. ET AL.: "Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa", ANIMAL REPRODUCTION SCIENCE, vol. 151, no. 3-4, 2014, pages 208 - 219, XP055489444
YOSHIMOTO, HIDETAKA ET AL., BIOLOGY OF REPRODUCTION, vol. 97, no. 6, 2017, pages 883 - 891
Attorney, Agent or Firm:
OSAWA Kenichi (JP)
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