Title:
COLLAGEN FUSION PROTEIN AND DRUG SCREENING METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2016/152882
Kind Code:
A1
Abstract:
The purpose of the present invention is to construct a system whereby secretion of procollagen from an animal cells can be detected. This purpose can be achieved by a fusion protein of a fluorescent protein or luminescent protein and a collagen-related protein which is a collagen protein, a procollagen protein, a preprocollagen protein having a repeating amino acid sequence of Gly-Xaa-Yaa (where Gly represents glycine and Xaa and Yaa represent arbitrary amino acids), or a variant or fragment thereof.
Inventors:
TANAKA TOSHIAKI (JP)
IKOMA TOSHIYUKI (JP)
TANAKA JUNZO (JP)
IKOMA TOSHIYUKI (JP)
TANAKA JUNZO (JP)
Application Number:
PCT/JP2016/059066
Publication Date:
September 29, 2016
Filing Date:
March 22, 2016
Export Citation:
Assignee:
TOKYO INST TECH (JP)
International Classes:
C12N15/09; C07K14/78; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/00; C12P21/02; C12Q1/02
Domestic Patent References:
| WO2004052934A1 | 2004-06-24 |
Foreign References:
| JP2004016144A | 2004-01-22 |
Other References:
CHUNG, H. J. ET AL.: "Fluorescent Protein Markers to Tag Collagenous Proteins; the Paradigm Procollagen VII", BIOCHEM BIOPHYS RES COMMUN, vol. 390, no. 3, 2009, pages 662 - 6, XP026770602
THEIN, M. C. ET AL.: "Caenorhabditis elegans Exoskeleton Collagen COL-19:An Adult-Specific Marker for Collagen Modification and Assembly, and the analysis od Organismal Morphology", DEVELOPMENTAL DYNAMICS, vol. 226, 2003, pages 523 - 39, XP055315687
MAYE, P. ET AL.: "Generation and Characterization of Coll10a1-mCherry Reporter Mice", GENESIS, vol. 49, 2011, pages 410 - 8, XP055315689
VENO, P. ET AL.: "Characterization of Collagen- GFP Transgenic Mice and Evidence for Osteocyte Deposition of Perilacunar Collagen", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 26, no. Supp.1, 2011
EMIKO ASHITAKA: "Cloning of the acceptor of sense of pain control peptide nosistatin and elucidation of the production structure.", RESEARCH REPORTS OF UEHARA MEMORIAL FOUNDATION, vol. 16, 2002, pages 257 - 259
THEIN, M. C. ET AL.: "Caenorhabditis elegans Exoskeleton Collagen COL-19:An Adult-Specific Marker for Collagen Modification and Assembly, and the analysis od Organismal Morphology", DEVELOPMENTAL DYNAMICS, vol. 226, 2003, pages 523 - 39, XP055315687
MAYE, P. ET AL.: "Generation and Characterization of Coll10a1-mCherry Reporter Mice", GENESIS, vol. 49, 2011, pages 410 - 8, XP055315689
VENO, P. ET AL.: "Characterization of Collagen- GFP Transgenic Mice and Evidence for Osteocyte Deposition of Perilacunar Collagen", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 26, no. Supp.1, 2011
EMIKO ASHITAKA: "Cloning of the acceptor of sense of pain control peptide nosistatin and elucidation of the production structure.", RESEARCH REPORTS OF UEHARA MEMORIAL FOUNDATION, vol. 16, 2002, pages 257 - 259
Attorney, Agent or Firm:
MORITA Kenichi et al. (JP)
Ken-ichi Morita (JP)
Ken-ichi Morita (JP)
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