FIELD OF THE INVENTION [0002] The present invention relates to combinatorial libraries of autoinducer analogs, methods using such combinatorial libraries, autoinducer agonists and antagonists, and methods of use thereof BACKGROUND OF THE INVENTION [0003] Pseudomonas aeruginosa is a ubiquitous gram-negative bacterium capable of infecting a wide variety of animals, plants, and insects (Rahme et al., Science 268: 1899-1902 (1995) ). P. aeruginosa is classified as an opportunistic pathogen because while it rarely causes disease in healthy people, it does infect people with impaired host defense systems that include cystic fibrosis (CF), cancer, AIDS, diabetes, deep burns, and wounds (Van Delden et al., Emerg.

Infect. Dis. 4: 551-560 (1998) ). During infection, P. ae°uginosa produces numerous virulence factors that cause tissue damage. In the case of CF, P. aeruginosa causes chronic, fatal lung infections in over 90% of patients (Hoiby, Acta. Path. Microbiol. Scand. Sect. B 82 : 551 (1974); Mahenthiralingam et al., Infectlmmun. 62: 596-605 (1994); Smith et al., Cell 85 : 229-236 (1996)).

Although several anti-pseudomonal antibiotics, such as 8-lactams, aminoglycosides and quinolones, are used in clinical drug regimens (Kovacs et al., Infect. Med. 15: 467-472 (1998)), P. aeruginosa frequently develops resistance to these treatments (Oliver et al., Science 288: 1251-1253 (2000) ). Compounding the difficulty in eliminating the infection is the intrinsic ability of P. aeruginosa to develop a biofilm (Costerton et al., Science 284: 1318-1322 (1999)). A biofilm is

a complex, polysaccharide-laden microniche that protects the bacteria from both antibiotics and the host's humoral and cell-mediated responses.

[0004] Regulation of virulence factor production and biofilm development in P. aeruginosa is controlled by a sophisticated inter-cellular signaling mechanism responding to cell population density, known as quorum sensing (QS) (de Kievit et aL, Sci. Med. Nov/Dec: 42-50 (1999); Passador et aL, Science 260: 1127-1130 (1993); Passador et al., J. Bacterial 178: 5995-6000 (1996); Hastings et al., J. Bacteriol. 181: 2667-2668 (1999); Pesci et al., Proc. Natl Acad.

Sci. USA 96: 11229-11234 (1999); Parsek et al., Proc. Natl Acad. Sci. USA 97: 8789-8793 (2000); Whiteley et al. , Proc. Natl Acad. Sci. USA 96: 13904-13909 (1999) ). This regulatory circuit was first discovered in the marine bacterium Vibriofischeri (Dunlap et al., J. BacterioL 164: 45-50 (1985) ), but later found in P. aerugifaosa and many other bacteria (Hastings et al., J. Bacteriol. 181: 2667-2668 (1999); de Kievit et al., Infect. Immun. 68: 4839-5862 (2000)). The QS system generally consists of two families of proteins, R (regulator) and I (autoinducer synthase) proteins. The R proteins are known to activate transcription of both R and I genes that encode these protein families, as well as numerous downstream targets that include virulence factor and biofilm genes. In contrast, the I proteins are synthases of autoinducer signal molecules (vide infra). Importantly, the R protein is"activated"for DNA binding and transcriptional activation only upon binding to its cognate autoinducer (Gambello et al., J. Bacteriol. 173: 3000-3009 (1991) ), which is synthesized by the respective I protein. Therefore, as more autoinducers are produced by the I proteins, more activated R proteins exist in the system, which results in increased I protein levels. Thus, it is no coincidence that the signaling molecules are called autoinducers, because of the feedback role they play in QS. In P. aeruginosa, two R proteins, known as LasR and RhlR, and two I proteins, known as LasI and RhlI, have been identified (Passador et al., Science 260: 1127-1130 (1993); Gambello et aL, J. Bacteriol. 173: 3000-3009 (1991); Ochsner et al., J. Bacterial 176: 2044-2054 (1994); Ochsner et al., Proc. Natl Acad. Sci. USA 92: 6424-6428 (1995) ). Two autoinducers that bind to the respective R proteins have been identified, and are known as N- (3- oxododecanoyl) -L-homoserine lactone (3-oxo-Cl2-HSL) (Pearson et al., Proc.

Natl Acad. Sci. USA 91: 197-201 (1994); Seed et al., J. Bacteriol. 177: 654-659 <BR> <BR> <BR> <BR> (1995) ) and N-butyloyl-L-homoserine lactone (C4-HSL) (Pearson et al., Proc. Natl Acad. Sci. USA 92: 1490-1494 (1995); Winson et al., Proc. Natl Acad. Sci. USA 92: 9427-9431 (1995) ). For simplicity, hereafter 3-oxo-Cl2-HSL and C4-HSL are called All and AI2, respectively. By means of these diffusible autoinducers, the bacterial cells can monitor cell density in their immediate surroundings, and regulate gene expression accordingly.

[0005] While the las and rhl QS system consists of two separate regulons, their functions are apparently not independent. Even though both AIl-LasR and AI2-RhIR can activate transcription of lasB (which encodes the elastase (LasB) virulence factor), it has been shown that the las QS system controls the rhl QS system at both the transcriptional (Pearson et al., J. Bacteriol. 179: 5756-5767 <BR> <BR> <BR> <BR> (1997) ) andpost-translational (Pesci et al., J. Bacteriol. 179: 3127-3132 (1997)) levels. For example, as cell density increases, All builds to a critical concentration, at which point it interacts with LasR (Passador et al., Science 260: 1127-1130 (1993) ). This AIl-LasR complex then activates transcription of a number of genes, such as lasB, toxA, rA, and lasI (Whiteley et al., Proc. Natl Acad. Sci. USA 96: 13904-13909 (1999); Gambello et al., J. Bacteriol. 173: 3000- 3009 (1991); Pesci et al., J. Bacteriol. 179: 3127-3132 (1997); Storey et al., Infect.

Immun. 66: 2521-2528 (1998); Toder et al., Infect. Immun. 62: 1320-1327 (1994); Gambello et al., Infect. Immun. 61: 1320-1327 (1993) ). This indicates the two systems are arranged in a hierarchy where the las QS system is dominant over the rhl QS system. Since activation of the lasI gene by the All-LasR complex essentially triggers the initial steps in the cascade, LasR is considered the master regulator of P. aeruginosa QS.

[0006] More recently, a third signal molecule, 2-heptyl-3-hydroxy-4- quinolone (Pseudomonas quinolone signal, PQS), has been found to provide a possible additional link between the las and rhl QS networks (Pesci et al., Proc.

NatlAcad. Sci. USA 96: 11229-11234 (1999); McKnight et al., J. Bacteriol.

182: 2702-2708 (2000) ). These studies showed that PQS strongly induces roll while it has lesser positive effects on the transcription of lasR and rhlR. Still, the

mechanism by which PQS influences the las and rlzl QS networks and the proteins involved in PQS biosynthesis are unknown.

[0007] The QS system has been shown to directly relate to bacterial pathogenicity. In particular, a number of reports have shown a positive correlation between QS and P. aeruginosa virulence (Costerton et al., Science 284: 1318-1322 (1999); de Kievit et al., Sci. Med. Nov/Dec: 42-50 (1999); Passador et al., Science 260: 1127-1130 (1993); de Kievit et al., Infect. Immun.

68 : 4839-5862 (2000); Storey et al., Infect. Immun. 66: 2521-2528 (1998); Hassett et al., MoL Microbiol. 34: 1082-1093 (1999); Pearson et al., Infect. Immun.

68: 4331-4334 (2000); Singh et al., Nature 407: 762-764 (2000); Rumbaugh et al., Infect. Immun. 67: 5854-5862 (1999); Tang et al., Infect. Immun. 64: 37-43 (1996); Davies et al., Science 280 : 295-298 (1998); Tan et al., Proc. Natl Acad. Sci. USA 96: 715-720 (1998); Tan et al. , Proc. Natl Acad.. Sci. USA 96: 2408-2413 (1999); Sawa et al., Infect. Immun. 66: 3242-3249 (1998); Telford et al., Infect. Immun.

66: 36-42 (1998); Saleh et al., Infect. Immun. 67: 5076-5082 (1999); Wu et al., Microbiol. 147: 1105-1113 (2001) ; Silo-Suh et al., Proc. Natl Acad. Sci. USA 99: 15699-15704 (2002); Lesprit et al., American Journal of Respiratory and Critical Care Medicine epub (2003)), for review see (Rumbaugh et al., Microbes and Infection 2: 1721-1731 (2000) ). Disruption of QS genes leads to decreased virulence in mouse, plant, nematode, Drosophila, and wax moth pathogenicity studies. For example, Hamood et al. have shown that the in vivo virulence of lasl, lasR, and rlzli mutants in a burned-mouse model (a commonly used mammalian pathogenicity model) was significantly reduced compared to the wild-type strain with an intact QS cascade (Rumbaugh et al., Infect. Immun. 67: 5854-5862 (1999) ). While the wild-type strain killed 94% of infected mice, the lasR mutant was able to kill only 28%, and the lasIrhlI double mutant killed only 7%.

Expression in trans of constitutively active lasI and rhll genes in the lasIrAlI mutant restored the ability of the organism to (i) spread within the burned tissue and systemically, and (ii) cause death at near wild-type levels (93%). Ausubel et al. have utilized a C. elegans-P. aeruginosa pathogenesis system to identify virulence-associated genes, and demonstrated that a lasR mutant is significantly less virulent in this surrogate model system (Tan et al., Proc. Natl Acad. Sci. USA

96: 715-720 (1998); Tan et al. , Proc. Natl Acad. Sci. USA 96: 2408-2413 (1999); Mahajan-Miklos et al., Cell 96 : 47-56 (2003) ). Interestingly, the QS mutants behave similarly to mutants with gene disruptions in crucial virulence factor genes. This is the expected result if indeed QS controls expression of virulence factors required for the disease process. It is interesting to note that interference with PQS production led to decreased expression of the LasB elastase virulence factor, further evidence that disruption of the QS system leads to decreased virulence (Calfee et al., Proc. NatlAcad. Sci. USA 98: 11633-11637 (2001)).

[0008] Another critical discovery was the overlapping role of virulence factors that spanned different phyla (Rahme et al., Science 268: 1899-1902 (1995); Tan et al., Proc. Natl Acad. Sci. USA 96: 2408-2413 (1999); Mahajan-Miklos et al. , Cell 96: 47-56 (2003); Rahme et al., Proc. NatlAcad. Sci. USA 94: 13245- 13250 (1997); Plotnikova et al., PlantPhysiol. 124: 1766-1774 (2000) ). The same P. aeruginosa mutants with reduced virulence in the worm, C. elegant, were also less virulent in the plant and burned mouse models. Based upon such experiments, it was postulated that the bacteria use the same virulence factors for infection of all hosts, a mode dependent upon QS.

[0009] A likely explanation for the decrease in virulence when QS is disrupted is that the QS transcriptional activators, LasR and RhIR, directly activate expression of genes encoding critical enzymes (LasB elastase, LasA protease, exoenzyme S, alkaline protease, and phospholipase C) and toxins (rhamnolipid, exotoxin A, cyanide, and pyocyanin) that contribute to the disease process (Rumbaugh et al., Microbes and Infection 2: 1721-1731 (2000); de Kievit et al., Sci. Med. Nov/Dec: 42-50 (1999); Whiteley et al., Proc. Natl Acad. Sci. USA 96: 13904-13909 (1999) ). QS also regulates components of the MexAB-OprM multidrug efflux pump (Evans et al., J. Bacteriol. 180: 5443-5447 (1998) ) and genes involved in resistance to reactive oxygen intermediates (sodA, sodB, and katA) (Hassett et al., Mol. Microbiol. 34: 1082-1093 (1999) ). Interestingly, the ability of P. aeruginosa to form complex, highly developed biofilms is also controlled by QS. Strains unable to form biofilms are less infectious. Davies et al. showed that a lasI mutant produces abnormal (thin and much more uniform) biofilms that are not resistant to detergent treatment. However, in the presence of

exogenous All, this mutant forms normal detergent-resistant biofilms of an average thickness and cell density similar to that of the wild-type biofilms (Davies et al., Science 280: 295-298 (1998) ). Singh et al. recently demonstrated that P. aeruginosa in sputum of CF patients are living in the biofilm mode of growth. A direct correlation was made that revealed in vitro biofilms and CF sputum harbored 3 times the AI2 relative to All levels (Singh et al., Nature 407: 762-764 (2000) ), implicating the rhl system in playing a critical role in the biofilm mode of growth. Wu et al. confirmed these results, showing that acyl-HSL molecules are not only expressed by P. aeruginosa infecting mouse lung tissue, but their presence in the lung coincides with the occurrence of pathological changes (Wu et al. , Microbiol. 146: 2481-2493 (2000) ). This strongly suggests that biofilms contribute to the chronic and refractory nature of P. aeruginosa infections in CF lungs, and biofilm formation is dependent on QS. Of particular importance is a study of sputa from lungs of CF patients by Storey et al. which found a direct correlation between the level of lasR mRNA and the level of LasB elastase, LasA protease, exotoxin A, and possibly algD (a gene involved in alginate synthesis, a major component of the biofilm matrix) MARNA (Storey et al., Infect. Izflmun.

66: 2521-2528 (1998)). These studies provide the first direct evidence that QS is indeed active in human lung infections. However, drugs designed to fight P. aeruginosa and QS pathogens based on the QS pathway have not been identified.

[00101 In the last few years, investigations of synthetic autoinducer analogs, with respect to agonist and antagonist activities in the QS systems of P. aeruginosa (Passador et al., J. Bacteriol. 178: 5995-6000 (1996); Kline et al., Bioorg. Med. Cliem. Lett. 9: 3447-3452 (1999)), V fischeri (Schaefer et al., J.

Bacteriol. 178 : 2897-2901 (1996)), andA. tumefaciens (Zhu et al., J Bacteriol.

180 : 5398-5405 (1998) ), have been reported. In these studies, major efforts were made to vary the length of the aliphatic side chain or modify the 3-oxo functionality of the autoinducers (substituting the 3-oxo group with 3-hydroxyl or methylene groups). The analog molecules whose structures are closely related to the cognate autoinducer structure exhibited weak-to-moderate gene activation (agonist activity). In some cases, the analogs weakly inhibited the induction of

target genes (antagonist activity). These studies, however, failed to identify potent synthetic antagonists.

[0011] Interestingly, secondary metabolites isolated from the Australian macroalga, Delisea pulchra, exhibited moderate antagonist activity against QS- controlled gene expression in Serratia liquefaciens, Z fischeri, and Eravinia carotovora, all gram-negative bacteria with QS systems similar to that of P. aeruginosa (Givskov et al., J. Bacteriol. 178: 6618-6622 (1996); Manefield et al., Microbiol. 145: 283-291 (1999), Rasmussen et aL, Microbiol. 146: 3237-3244 (2000) ; Manefield et aL, FEMSMicrobiol. Lett. 205: 131-138 (2001) ). These natural products are highly halogenated furanone derivatives that are able to act as an antagonist of an A12-like autoinducer in S. liquefaciens and 3-oxo-C6-HSL in both V fischer and E. carotovora. Although their structure, in terms of the electronic and steric features, significantly differs from that of the HSL autoinducers, it still shares the features of the aliphatic side chain and the five- membered lactone ring (but fully conjugated) with the HSL autoinducers. The above naturally occurring furanone was nearly inactive in wild type of P. aeruginosa, PAO1. In 2002, Hentzer et al. reported that a furanone that lacks the alkyl side chain of the natural product exhibits antagonist activity using a PAO- JP2 mini-Tn5-PlasB-gfp (ASV) reporter system (Hentzer et al., Microbiol. 148: 87- 102 (2002) ). Approximately 50% inhibition was observed in the presence of 320 uM of the furanone competing with 0. 1 uM All. It should be noted that Whiteley et al. grouped QS promoters into four classes based on their responsiveness to All vs. AI2, and the degree and timing of that response (Whiteley et al., Proc. Natl Acad. Sci. USA 96: 13904-13909 (1999) ). The lasB promoter used in the Hentzer's study is a Class IV promoter that requires both All and AI2 for full induction, and responds very little to All alone. Additionally, they used a low All concentration.

[0012] The present invention is directed to overcoming these and other deficiencies in the art.

SUMMARY OF THE INVENTION [0013] The present invention relates to combinatorial libraries of autoinducer analogs as described below. The combinatorial libraries include solid phase (i. e. , members of the library are attached to a solid support) and solution phase (i. e. , members of the library are present in solution) libraries.

[0014] Another aspect of the present invention relates to an autoinducer agonist or antagonist having the structure

wherein Xi is selected from the group consisting of H and OH, Xa is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR\ Suri, NHa, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Ri and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl and n is 0 to 4; or

wherein X3 is independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl and n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl aryl heteroaryl, carbonyl, and sulfonyl ; or

wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+l, wherein m is 1 to 14, wherein p is 1 to 14, and wherein q is 1 to 14, X4 is OH, NH2, or SH and Xs is H, or X4 is H and X5 is OH, NH2, or SH.

[0015] Compositions including the autoinducer analog (s) of the present invention are also disclosed and may also include an antibiotic.

[0016] Yet another aspect of the present invention relates to a method of identifying an autoinducer agonist. This method involves providing an autoinducer analog having the structure

wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alky, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2al7 wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NHz, or SH and Xs is H, or X4 is H and X5 is OH, NH2, or SH. The autoinducer analog is contacted with an autoinducer receptor and activity of the receptor is measured in the presence of the autoinducer analog.

[0017] A further aspect of the present invention relates to a method of identifying an autoinducer antagonist. This method involves providing an autoinducer analog having the structure wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R and R are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xl, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or

wherein Xa and X3 are independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+l, wherein m is 1 to 14, wherein p is 1 to 14, and wherein q is 1 to 14, X4 is OH, NH2, or SH and Xs is H, or X4 is H and Xs is OH, NH2, or SH. The autoinducer analog is contacted with an autoinducer receptor and an autoinducer, whereby competition between the autoinducer analog and the autoinducer for the autoinducer receptor is allowed to occur. Activity of the receptor in the presence of the autoinducer analog and autoinducer is then measured and compared with activity of the receptor in the presence of only the autoinducer.

[0018] Yet another aspect of the present invention relates to a method for regulating the activity of an autoinducer receptor. This method involves contacting an autoinducer receptor with an autoinducer analog, wherein the autoinducer analog is an autoinducer agonist or antagonist. In one embodiment, the autoinducer analog has the structure

wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl,

heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or

wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro,

halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+i, wherein m is 1 to 14, wherein p is 1 to 14, and wherein q is 1 to 14, X4 is OH, NH2, or SH and X5 is H, or X4 is H and Xs is OH, NH2, or SH, whereby activity of the autoinducer receptor is regulated.

[0019] A further aspect of the present invention relates to a method of regulating biofilm formation. This method involves contacting a cell of an organism with an autoinducer analog as described herein, whereby biofilm formation on the cell is regulated. In accordance with the present invention, biofilm formation may be inhibited or biofilm architecture may be modified by contact with the autoinducer analog.

[0020] The present invention also relates to a method for regulating the growth or virulence of an organism in a subject. This method involves contacting a cell of an organism with an autoinducer analog as described herein, whereby growth or virulence of the organism in the subject is regulated.

[0021] Another aspect of the present invention relates to a method of inhibiting a quorum sensing mechanism in an organism. This method involves contacting a cell of an organism possessing a quorum sensing mechanism with an

autoinducer analog as described herein, whereby the quorum sensing mechanism of the organism is inhibited.

[0022] Yet another aspect of the present invention relates to a method of treating an infection in a subject caused by an organism possessing a quorum sensing mechanism. This method involves administering to the subject an effective amount of a composition including an autoinducer analog as described herein and a pharmaceutically acceptable carrier or diluent.

[0023] In accordance with the present invention, combinatorial libraries of autoinducer analogs can be prepared. Such libraries can be used to identify antagonists that will inhibit the interaction between wild type autoinducer molecules and their cognate transcription regulator proteins, both of which play critical roles in regulating the quorum sensing cascade. The antagonists can be used in new therapies to fight infection by P. aeruginosa as well as other organisms which use a quorum sensing system to regulate the production of numerous virulence factors and to form protective biofilms.

BRIEF DESCRIPTION OF THE DRAWINGS [0024] Figures 1A-B shows quorum sensing (QS) in P. aeruginosa.

Figure 1A shows a schematic representation of QS activation from low cell density to high cell density, to biofilm development (left), and the mechanism of two QS circuits, las and rhl (right). Light grey lines and arrows indicate specific activations by the complex of R proteins to the cognate autoinducers (AIs). Dark grey arrows indicate secretion of AIs or virulence factors to initiate the cell-to-cell communication or host attacks. Figure 1B shows the chemical structures of autoinducers found in P. aeruginosa QS circuits. All activates the las circuit, while AI2 activates the rhl circuit.

[0025] Figure 2 shows a solid phase library synthesis scheme of the present invention. Reagents and conditions: (a) n-BuLi, THF,-78 °C ; undecenoyl chloride. (b) HOCH2CH2OH, p-TsOH, benzene, reflux. (c) BH3. THF, THF; H202, NaHCO3 aq, 38% for three steps (a-c). (d) DHP resin, PPTS, CH2Ck, room

temperature, 27 hours. (e) LiOH in THF/water, 75 °C, 20 hours. (f) parallel couplings with 94 amines and 2 alcohols (X), EDC, DMAP, DIPEA, DMF, room temperature, 72 hours. (g) 95% TFA, room temperature, 30 minutes, R = CF3CO or H.

[0026] Figure 3 shows a solid phase library synthesis scheme of the present invention. Reagents and conditions: (a) BH3-THF, THF; H202, NaHCO3 aq.; (b) DHP resin, PPTS, CH2C12, room temperature, 27 hours; (c) LiOH in THF/water, 75 °C, 20 hours; (d) Meldrum's acid, DCC, DMAP, CH2C12 ; (e) parallel couplings with amines or alcohols (X), Et3N, CH3CN, reflux; (f) 95% TFA, room temperature, 30 minutes, R = CF3CO or H.

[0027] Figure 4 shows the structures of 96 molecules used for parallel synthesis in accordance with the present invention. The Aldrich/Sigma catalog numbers are listed under the molecules.

[0028] Figures 5A-B show agonist screening. Figure 5A is a 96-well plate assay of PAO-JP2 (plasl-LVAgfp) in the presence of library compounds.

Negative control is no treatment. Positive control is 1 uM All. Each well of the plate contains roughly 400 u, M of one library compound. Figure 5B shows the structures of compounds in wells with GFP expression and related compounds lacking agonist activity.

[0029] Figure 6 shows the structure of antagonists and similar compounds with agonist or no activity.

[0030] Figure 7 is a schematic showing the inhibition of quorum sensing that attenuates pathogenicity. Anta abbreviates antagonist.

[0031] Figure 8 shows representative on-resin FT-IR spectra of solid phase reactions. Only the region between 1000 cri 1 an 2000 cell ils shown here.

[0032] Figures 9A-B show reporter gene assays. Figure 9A shows PAO- JP2 (plasl-LVAgfp) expression of GFP in the presence of increasing amounts of designated compounds. Figure 9B shows MG4 (pKDT37) beta-galactosidase activity in the presence of increasing amounts of designated compounds.

[0033] Figures 1OA-C show the chemical structure of an antagonist and antagonist assays. In Figure 10B, PAO-JP2 (plasl-LVAgfp) was grown for 6 hours in the presence of 1 uM All alone (white) or with 3-oxo-Cl2-D10 competitor (dark grey). In Figure 10C, PAO-JP2 (prhll-LVAgfp) was grown for 6 hours in the presence of 1 uM AI1 and 10 µM AI2 alone (white) or with 3-oxo- C12-D 10 (dark grey) or C4-D10 (grey). Fluorescence intensity was divided by OD600 of cell culture. Control was set to 1 and all other values are relative.

[0034] Figures 11A-D show virulence factor assays. In Figure 11A, PAO- JP2 was grown for 6 hours in the absence (negative control, white) or presence (positive control, grey) of 5 µM AI1 and 10 uM AI2 or in combination with 3- oxo-Cl2-D10 (dark grey), and elastase activity was determined. In Figure 11B, wild type strain PAO1 was grown without (grey) or with antagonist (dark grey).

In Figure 11C, PAO-JP2 was grown for 18 hours in the absence (negative control, white) or presence (positive control, grey) of 25 uM All and 25, uM AI2 or in combination with 3-oxo-Cl2-D10 (dark grey) then assayed for pyocyanin production. In Figure 1 1D, PA01 was grown without (grey) or with antagonist (dark grey) then assayed for pyocyanin production.

[0035] Figures 12A-E show static biofilm assays. Figures 12A-C show PAO-JP2 (pTdK-GFP) (de Kievit et al., Appl. Environ. Microbiol. 67: 865-1873 (2001), which is hereby incorporated by reference in its entirety) biofilm development in the presence of designated compounds. Figures 12A and B represent negative and positive controls. Figures 12D and E show the absence (negative control) or presence of antagonist, respectively.

[0036] Figure 13 shows the chemical structures of autoinducers and analogs. All, 3-oxo-Cl2HSL, AI2, C4 HSL; 1, 3-oxo-Cl2-(2- aminocyclopentanone); 2, 3-oxo-Cl2- (2-aminocyclopentanol) ; 3, 3-oxo-Cl2-(2- aminocyclohexanone) ; 4 (formerly called 3-oxo-Cl2-D12 in Figure 5), 3-oxo-C12- (2-aminocyclohexanol) ; 5, C4- (2-aminocyclopentanone), 6, C4- (2- aminocyclopentanol); 7, C4- (2-aminocyclohexanone) ; 8, C4- (2- aminocyclohexanol).

[0037] Figures 14A-D show reporter gene induction by autoinducers and their analogs. Figure 14A shows a Molecular Imager scan of a 96-well plate containing PAO-JP2 (plasI-LVAgfp) in the presence of 1,100, and 400 u, M of the designated 3-oxo-Cl2 compound. Figure 14B shows the quantification of results of 3-oxo-Cl3 compounds. Cont, negative control of untreated cell (not shown in Figure 14A). Average fluorescence of four replicate wells was corrected for cell density by dividing by OD6oo of cell culture. The standard deviation was derived from three independent experiments shown in Figure 14A. Figure 14C shows a Molecular Imager scan of a 96-well plate containing PAO-JP2 (pf-hll-LVAgfp) in the presence ofl uM AI2 and 10,100, and 400 uM designated C4 compound.

Figure 14D shows the quantification of results of C4 compounds. Data analysis was the same as in Figure 14B.

[0038] Figures 15A-B show antagonist assays. Figure 15A shows GFP expression by PAO-JP2 (plasI-LVAgfp) in the presence of 1, uM AI1 alone (control, black bar) or 1 µM AI1 plus 2 or 3. Figure 15B shows GFP expression by PAO-JP2 (prhlI-LVAgfp) in the presence of l, uM AI1 and 10 M AI2 (control, black bar) or 1 uM All and 10, M AI2 plus2 or 3.

[0039] Figures 16A-E show virulence factor assays. Figure 16A shows pyocyanin expression by PAO-JP2. -, negative control; +, positive control, 25 uM each of All and AI2 ; 2, 25 µM each of All and AI2 in the presence of 100 uM 2; 3, 25 uM each of All and AI2 in the presence of 100 uM 3. Figure 16B shows the quantification of pyocyanin expression by PAO-JP2 in the presence of various concentrations of 2 or 3. -, negative control containing no AIs ; +, positive control containing 25 pM each of All and AI2 ; 2, 25 uM each of All and AI2 in the presence of 2; 3, 25 uM each of All and AI2 in the presence of 3. Figure 16C shows the quantification of pyocyanin expression of wild type strain PAO1. The conditions were the same as Figure 16B, except for control where no AIs were added. Figure 16D shows elastase B activity produced by PAO-JP2 in the absence (-control) or presence of 5 gM All and 10 uM AI2 (+ control) or 5 uM All and 10 uM AI2 plus 2 or 3. Figure 16E shows elastase B activity produced by PA01 control or in the presence of 2 or 3.

[0040] Figures 17A-B show a static biofilm assay. Figure 17A shows PAO-JP2 (pTdK-GFP) biofilm development in the presence of designated compounds. Figure 17B shows PAO-1 (pTdK-GFP) biofilm development in the absence (negative control) or presence of 50 uM 3.

[0041] Figure 18 shows agonist and antagonists in P. aeruginosa QS circuits found in accordance with the present invention.

[0042] Figure 19 shows the synthesis of chiral aminoalcohols. Reagents and conditions: (a) (i) 2 mol% Jacobsen catalyst, TMSN3 ; (ii) MeOH, cat TFA; (iii) 2 mol% PtO2, Ho; (b) (i) Ac20 ; (ii) SOC12 ; (iii) HC1, heat.

[0043] Figure 20A shows the synthesis of las antagonist focused libraries.

Figure 20A shows solid phase synthesis of the library based on 3- aminocyclohexene. Reagents and Conditions: (a) (i) PPTS; (ii) mCPBA ; (b) ) CM+ or Lewis acid with TMS-X; (c) (i) TFA; (ii) Dess-Martin reagent. Figure 20B shows epoxy and chloro-substituted suicide compounds.

[0044] Figures 21A-B show aniline derivatives. In Figure 21 A, Xi, X2, and X3 can be any functional group. Figure 21B shows aniline derivatives with expanded rings.

DETAILED DESCRIPTION OF THE INVENTION [0045] The present invention relates to a combinatorial library of autoinducer analogs, including two or more different autoinducer analogs of the structure

wherein Xi is independently selected from the group consisting of H and OH, X2 is independently selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR1, SR1, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, # is a solid support, and n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, OR', SR1, NH2, NHR', NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, # is a solid support, and n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NHa, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkenyl aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and # is a solid support; or

wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and is a solid support, and wherein R is selected from the group consisting of CmH2m, wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NH2, or SH and X5 is H, or X4 is H and X5 is OH, NH2, or SH. The side chain (CmH2m, Cfp), or C (q)) in the R group may include saturated and unsaturated bonds and may be substituted or unsubstituted. Suitable substitutions are described below with respect to alkyl groups in general. In this embodiment of the present invention, the combinatorial library is a solid phase library.

[0046] As used herein, the term alkyl refers to a saturated aliphatic hydrocarbon include straight chain and branched chain groups. Preferably, the alkyl group has from 1 to 12 carbon atoms. The alkyl group may be substituted or unsubstituted. Suitable substituents include, but are not limited to, cycloalkyl, aryl, heteroaryl, heteroalicyclic, hydroxy, alkoxy, aryloxy, thiohydroxy, thioalkoxy, thioaryloxy, cyano, halogen, carbonyl, thiocarbonyl, carboxy, nitro, silyl, and amino. A cycloalkyl group refers to an all carbon monocyclic or fused ring (i. e. , rings which share an adjacent pair of atoms) group wherein one or more of the rings does not have a completely conjugated pi-electron system. Suitable cycloalkyl groups include, but are not limited to, cyclopropane, cyclobutane, cyclopentane, cyclopentene, cyclohexane, cyclohexadiene, cycloheptane, and cycloheptatriene. A cycloalkyl group may be substituted or unsubstituted.

Suitable substituents include those describe above an alkyl groups.

[0047] As used herein, an aryl group refers to an all carbon monocyclic or fused-ring polycyclic group having a completely conjugated pi-electron system.

Suitable examples of aryl groups include, but are not limited to, phenyl, benzyl, benzoyl, naphthalenyl, and anthracenyl. The aryl group may be substituted or unsubstituted. Suitable substituents include, but are not limited to, alkyl, hydroxy, alkoxy, aryloxy, thiohydroxy, thioalkoxy, thioaryloxy, cyano, nitro, carbonyl, thiocarbonyl, carboxy, sulfinyl, sulfonyl, amino, halogen, and triohalomethyl.

[0048] As used herein, a heteroaryl group refers to a monocyclic or fused ring group having in the ring (s) one or more heteroatoms, such as sulfur, nitrogen, and oxygen, and in addition having a completely conjugated pi-electron system.

Suitable heteroaryl groups include, but are not limited to, pyrrole, furan, thiophene, imidazole, oxazole, thiazole, pyrazole, pyridine, pyrimidine, quinoline, isoquinoline, purine, and carbazole. The heteroaryl group may be substituted or unsubstituted. Suitable substituents include, but are not limited to, alkyl, cycloalkyl, hydroxy, alkoxy, aryloxy, thiohydroxy, thioalkoxy, thioaryloxy, cyano, nitro, carbonyl, thiocarbonyl, carboxy, sulfinyl, sulfonyl, amino, halogen, and triohalomethyl.

[0049] As used herein, a heteroalicyclic group (or heterocycloalkyl) refers to a monocyclic or fused ring group having in the ring (s) one or more heteroatoms, such as sulfur, nitrogen, and oxygen. The rings may also have one or more double bonds. However, the rings do not have a completely conjugated pi-electron system. The heteroalicyclic ring may be substituted or unsubstituted.

Suitable substituents include, but are not limited to, alkyl, cycloalkyl, hydroxy, alkoxy, aryloxy, thiohydroxy, thioalkoxy, thioaryloxy, cyano, nitro, carbonyl, thiocarbonyl, carboxy, sulfinyl, sulfonyl, amino, halogen, and triohalomethyl.

[0050] A second embodiment of the present invention relates to a combinatorial library including two or more different autoinducer analogs of the structure

wherein Xi is independently selected from the group consisting of H and OH, X2 is independently selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR1, Suri, NHa, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, OR', SR1, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R and R are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR1, Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl,. carbonyl, and sulfonyl, and wherein R is selected from the group consisting of C. H2. +l, wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NH2, or SH and X5 is H, or X4 is H and Xs is OH, NH2, or SH. Thus, in this embodiment of the present invention, the combinatorial library is a solution phase library.

[0051] As used herein an autoinducer is a molecule encoded by an autoinducer synthase gene (e. g. , I gene) in a quorum sensing pathway of an organism. Figures 1A-B show the quorum sensing pathway in P. aeruginosa and the generation of two autoinducer molecules, All and AI2, by the I genes LasI and RhII. As used herein, a"combinatorial library"is an intentionally created collection of differing molecules which can be created by the techniques set forth below or otherwise and screened for activity in a variety of formats (e. g. , libraries of soluble molecules or libraries of compounds attached to a solid support). A "combinatorial library"involves successive and/or parallel rounds of chemical synthesis based on a common starting structure. The combinatorial libraries can be screened in a variety of assays, such as those described below as well as other assays useful for assessing biological or chemical activities. The combinatorial libraries will generally have at least one active compound and are generally prepared such that the compounds are in equimolar quantities. Thus, in one embodiment of the present invention, the combinatorial library includes at least one compound which is active as an autoinducer agonist or an autoinducer antagonist. Compounds disclosed in the prior art that are not in an intentionally created collection are not part of a"combinatorial library"of the present invention. In addition, compounds that are part of an unintentional or undesired mixture are not part of a"combinatorial library"of the present invention.

[0052] A combinatorial library in accordance with the present invention can include any number of different autoinducer analogs. As used herein an autoinducer analog is different if one autoinducer analog includes an R group, an Xi group, an X2 group, or an X3 group which is not present in the other autoinducer analog or an n value which is different than the other autoinducer analog. In one embodiment, the combinatorial library contains at least about 1,000 to 10,000 different autoinducer analogs. l0053] The combinatorial library of autoinducer analogs of the present invention encompasses autoinducer analogs for any organism having a quorum sensing pathway. Such organisms include gram negative and gram positive bacteria. Suitable examples include, but are not limited to, Pseudomonas aeruginosa, Aeromonas hydrophilia, Aeromonas sal2onicida, Yersinia <BR> <BR> <BR> <BR> pseudotuberculosis, Helicobacter pylori, Agrobacterium tumefaciens, Vibrio<BR> <BR> <BR> <BR> <BR> <BR> <BR> f scheri Vibrio harveyi, Erlwinia carotovora, Rhizobium leguminosarum, Rhodobacter sphaeroides, and Escherichia coli. In one embodiment, the combinatorial library of the present invention encompasses autoinducer analogs for Pseudomonas aeruginosa.

[0054] In accordance with the present invention, the combinatorial library synthesis can be carried out either manually or through the use of an automated process. For manual synthesis, the chemical manipulations would be performed by a scientist or technician. For automated synthesis, the chemical manipulations would typically be performed robotically. The choice and implementation of such techniques is within the skill of one of ordinary skill in the art of combinatorial chemistry and will not be discussed in detail herein.

[0055] As described in detail herein, the synthesis of an autoinducer analog combinatorial library of the present invention can be performed on a solid support. As used herein, a solid support is a insoluble substrate that has been appropriately derivatized such that a chemical molecule can be attached to the surface of the substrate through standard chemical methods. Suitable solid supports include, but are not limited to, beads and particles. The solid support can include many different materials which are capable of being functionalized through synthetic methods or already include a suitable functional group.

Examples of such materials include, but are not limited to, polymers, plastics, resins, polysaccharides, silicon or silica based materials, carbon, metals, inorganic glasses, and membranes. In one embodiment, the solid support is a 3,4-dihydro- 2H-pyran-2-ylmethoxymethyl polystyrene (DHP resin). Other preferred solid supports include those which allow attachment of a hydroxyl group.

[0056] As described above, the solid support can be provided with a suitable functionality already present or the solid support can be chemically modified such that a desired chemical molecule is attached to the support surface.

The choice of functionality used for attaching a chemical molecule to the solid support will depend on the nature of the molecule to be attached and the type of solid support. Examples of suitable functionalities on a solid support used to attach a chemical molecule to the solid support include, but are not limited to, alkyl or aryl halides, aldehydes, alcohols, ketones, amines, sulfides, carboxyl groups, aldehyde groups, and sulfonyl groups. Preferably, the functional group on the solid support in the present invention is an alcohol.

[0057] For the attachment of certain molecules to the solid support, masking of functionality that is not involved in the attachment process may be necessary. Strategies for the use of masking or protecting groups are well known in the art and will not be described in detail herein.

[0058] General synthetic strategies for the construction of autoinducer analog combinatorial libraries in accordance with the present invention are shown in Figures 2 and 3. It should be appreciated that the chemical compounds described herein are merely exemplary and other groups can be used (e. g., different alkyl side chain lengths can be used without changing the synthetic methods). In particular, with regard to Figure 2, an R group as set forth above (in this case a 3-oxo-C12 side chain) was immobilized onto a solid phase and coupling with various amines and alcohols was carried out in parallel. More specifically, ethyl 3,3-ethylenedioxo-13-hydroxy-tridecanoate (2) was synthesized in solution phase through a three-step sequence. The attachment of this molecule to DHP resin (3) and on-resin hydrolysis of the ester moiety (4) was then performed.

Parallel coupling of amines and alcohols having different structural features was accomplished on a 96 well reactor block. Suitable amines and alcohols are set

forth in Figure 4, however, this listing is merely representative. Any suitable molecule to produce a combinatorial library as described above may be used. The products are released from the resin by treatment with 95% TFA, which simultaneously removes the protective ketal group. The individual products are then collected into, for example, a 96 deep well plate, dried, and then passed through a tertiary alkyl amine resin to remove residual TFA.

[0059] With regard to Figure 3, an alternative synthesis scheme is set forth. In the methodology set forth in Figure 2, protection and deprotection steps of the 3-keto protecting group (cyclic ketal) are included. In contrast, in the methodology shown in Figure, 3, such steps are not required and, thus, the synthesis technique is shortened.

[0060] The synthesis for molecules without a 3-oxo group can be carried out in an analogous method as shown in Figure 3, using a different starting material (with additional carbon atoms) and substituting the methods and reagents in steps (d) and (e) of Figure 3 with those of step (f) in Figure 2. The remaining procedures are the same as those in Figure 3.

[0061] Thus, in one embodiment of the present invention, the autoinducer analog library is bound to a solid support. In another embodiment of the present invention, the autoinducer analog library is cleaved from the solid support.

Cleavage of compounds from a solid support to produce a soluble chemical library can be accomplished using a variety of methods including hydrolysis, as described above, as well as phytolytic cleavage, nucleophilic attack, and Lewis acid- catalyzed hydrolysis. Alternatively, techniques for solution phase synthesis of a combinational library may be used, as are known in the art (Edwards et al., Curr.

Opin. Drug Discov. Devel. 5 (4): 594-605 (2002), which is hereby incorporated by reference in its entirety). Solid supported libraries and solution phase libraries of the present invention may be used, for example, in the assays described below to identify autoinducer agonists and antagonists.

[0062] In another embodiment of the present invention, a position- scanning combinatorial approach is used to generate the combinatorial libraries of the present invention. Such techniques are generally known in the art. In particular, in this embodiment, once a structural motif (e. g., amine or alcohol

group) identified using a library generated, for example, using the synthetic scheme of Figure 2 or 3 is identified as possessing the desired activity (e. g., agonist or antagonist activity), a second generation library is constructed based on this information. In this second generation library, a different portion of the analog molecule (e. g. , the alkyl side chain and/or the 3-oxoamide group shown in structure (6) in Figure 2) is extensively varied into different structural elements while maintaining the previously identified structural motif (amine group or alcohol group) which displayed the desired activity. The new library (and any further libraries) can then be screened for increased activity. The number of compounds tested is the product of the number of varied structural elements in all of the libraries produced.

[0063] The autoinducer analogs produced in accordance with the present invention can be used to enhance bacterial growth by acting in concert with or instead of the wild-type autoinducer (i. e. , an autoinducer agonist). Alternatively, the autoinducer analog can inhibit the wild-type autoinducer by competing with the wild-type autoinducer in the quorum sensing pathway (i. e. , an autoinducer antagonist).

[0064] Thus, the present invention also relates to an autoinducer agonist or antagonist having the structure wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, n is 0 to 4; or

wherein X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X1, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or

wherein Xa and X3 are independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHRI, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+,, wherein m is 1 to 14, wherein p is 1 to 14, and wherein q is 1 to 14, X4 is OH, NH2, or SH and Xs is H, or X4 is H and Xs is OH, NH2, or SH. In one embodiment, n is 1 to 4.

[0065] Preferred autoinducer agonists of the present invention are set forth in Figure 5B and are described in detail in the Examples, below. Preferred autoinducer antagonists of the present invention are set forth in Figure 6 and are described in detail in the Examples, below. As described in detail below, autoinducer antagonists of the present invention can be used to attenuate the pathogenicity of an organism possessing the quorum sensing mechanism (see also Figure 7). Additional preferred autoinducer agonists and antagonists are set forth in Figure 18 and are described in Example 11, below. As described herein, in one embodiment, the agonists and antagonists have a hydrogen bond donor or acceptor adjacent to the amino group that connects to the R side chain.

[0066] The autoinducer analogs identified in accordance with the present invention (e. g. autoinducer agonists or antagonists) can be used in compositions which include one or more autoinducer analogs. In one embodiment, the composition also includes an antibiotic. Suitable antibiotics include bactericidal and bacteristatic agents, such as sulfonamides, anti-urinary tract agents, p-lactam antibiotics, quinolones, cephalosporins, clavulanic acid derivatives, aminoglycosides, carbapenems, tetracyclines and related antibacterial agents, macrolides, anti-tuberculosis drugs, anti-mycobacterium avium agents, anti- leprosy agents, antifungal agents, and antiviral agents. In this embodiment, the autoinducer analog is preferably an autoinducer antagonist. The autoinducer antagonist may be present in the composition in an amount of from about 10 ng/L to about 10 mg/L and the antibiotic may be present in the composition in an amount of from about 10 ng/L to about 10 mg/L, however, any desirable quantity of autoinducer antagonist or antibiotic may be used.

[0067] Such compositions are useful for inhibition of growth of an organism possessing a quorum sensing pathway. One example of such as use is in medical devices used both percutaneously and implanted within a patient. Such medical devices commonly serve as loci for bacterial infection. Thus, the autoinducer analogs and compositions of the present invention can be used to inhibit growth of an organism possessing a quorum sensing pathway on a medical device associated with non-invasive or invasive medical procedures. Such medical devices include, but are not limited to, catheters, pacemakers, vascular

grafts, vascular stents, dialysis membranes, heart valves, surgical instruments, blood bags, sutures, prostheses, dental devices, and artificial organs.

[0068] The autoinducer analogs and compositions can be incorporated (e. g. , impregnated or infused) within the structure of the medical device (e. g., chemically incorporated within an outer layer of a medical device) or provided as a coating on the medical device. Techniques suitable for incorporating the autoinducer analogs and compositions of the present invention within a medical device depend upon the particular autoinducer analog/composition used and the medical device used. Techniques suitable for providing a coating on a medical device substrate are well known in the art and will not be discussed in detail herein.

[0069] The medical device may be further supplemented with any other desirable compound, for example, antibiotics, analgesics, anticoagulants, anti- inflammatory compounds, antimicrobial compositions, vitamins, minerals, and the like.

[0070] The autoinducer analogs and compositions can also be used to inhibit growth of an organism possessing a quorum sensing pathway on any product or device that comes into contact with human (or other mammalian) tissue, such as a toothbrush or dental floss.

[00711 Moreover, the autoinducer analogs and compositions of the present invention may also be used as immune suppressive agents (see Telford et al., Infect. Immun. 66 (1) : 36-42 (1998), which is hereby incorporated by reference in its entirety).

[0072] Yet another aspect of the present invention relates to a method of identifying an autoinducer agonist. This method involves providing an autoinducer analog having the structure

wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein Xi, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR', Suri, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or wherein X2 and X3 are independently selected from the group consisting of H, OH, SH, OR1, SR1, NH2, NHR1, NR1R2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+1, wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NH2, or SH and Xs is H, or X4 is H and Xs is OH, NH2, or SH. In one embodiment, n is 1 to 4. The autoinducer analog is contacted with an autoinducer receptor and activity of the receptor is measured in the presence of the autoinducer analog. The autoinducer analog can be provided as part of a combinatorial library, as described above.

[0073] In a preferred embodiment, an autoinducer analog is identified using a organism strain lacking the ability to produce one or more natural autoinducers, due to the disruption of any autoinducer synthase genes. Thus, activation of autoinducer receptors in such strains relies on the addition of exogenous autoinducer. Such strains include, but are not limited to, P. aeruginosa PAO-JP2 and E. coli MG4 (pKDT37).

[0074] Activity of the receptor can be measured using any suitable reporter assay. In particular, a reporter may be incorporated into the above strain as is known in the art, such that the strain is capable of providing a signal (e. g., luminescence or enzyme activity) in the presence of autoinducer or an autoinducer agonist. Suitable reporters include, but are not limited to, gfp and lacZ. Activity of the receptor in the presence of the autoinducer analog can be compared to known activities of other autoinducer agonists or the autoinducer itself.

[0075] A further aspect of the present invention relates to a method of identifying an autoinducer antagonist. This method involves providing an autoinducer analog having the structure

wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR', SR1, NH2, NHRI, NRIR2, COORI, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, ORI, Suri, NH2, NHRI, NRIR2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R'and W are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein Xl, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, SR1, NH2, NHR1, NRIR2, COORI, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or wherein Xa and X3 are independently selected from the group consisting of H, OH, SH, ORt, Sort, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+l, wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NH2, or SH and Xs is H, or X4 is H and Xs is OH, NH2, or SH. In one embodiment, n is 1 to 4. The autoinducer analog is contacted with an autoinducer receptor and an autoinducer, whereby competition between the autoinducer analog and the autoinducer for the autoinducer receptor is allowed to occur. Activity of the receptor in the presence of the autoinducer analog and autoinducer is then measured and compared with activity of the receptor in the presence of only the autoinducer. Suitable reporter assays for use in this method of the present invention are described above. The autoinducer analog can be provided as part of a combinatorial library, as described above.

[0076] Yet another aspect of the present invention relates to a method for regulating the activity of an autoinducer receptor. This method involves contacting an autoinducer receptor with an autoinducer analog, wherein the autoinducer analog is an autoinducer agonist or antagonist. In one embodiment, the autoinducer analog is an autoinducer-1 (All) agonist or antagonist compound.

In another embodiment, the autoinducer analog is an autoinducer-2 (AI2) agonist or antagonist compound. In a further embodiment, the autoinducer analog has the structure

wherein Xi is selected from the group consisting of H and OH, X2 is selected from the group consisting of H and OH, X3 is independently selected from the group consisting of H, OH, SH, OR', SR1, NH2, NHRI, NR'R2, COORI, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein R1 and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or wherein X3 is independently selected from the group consisting of H, OH, SH, OR1, Suri, MH NHRI, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and n is 0 to 4; or

wherein Xl, X2, and X3 are independently selected from the group consisting of H, OH, SH, OR1, SR1, NH2, NHR1, NR1R2, COOR1, alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent Xi, X2, and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl ; or wherein Xa and X3 are independently selected from the group consisting of H, OH, SH, OR1, SR1, NHz, NHR1, NRIR2, COOL', alkyl, cycloalkyl, alkenyl, alkynyl, aryl, heteroaryl, heteroalicyclic, acyl group, carbonyl, cyano, nitro, halogen, and, adjacent X2 and X3 substituents combined, a cycloalkyl of 3 to 8 carbon atoms, a heterocycloalkyl of 3 to 8 members comprising carbon atoms and from 1 to 6 heteroatoms selected from the group consisting of N, S, and O, and an aryl or heteroaryl ring having 3 to 8 members, wherein Rl and R2 are selected from the group consisting of H, alkyl, cycloalkyl, alkenyl, aryl, heteroaryl, carbonyl, and sulfonyl, and wherein R is selected from the group consisting of CmH2m+1, wherein m is 1 to 14, wherein p is 1 to 14, and

wherein q is 1 to 14, X4 is OH, NH2, or SH and X5 is H, or X4 is H and X5 is OH, NH2, or SH, whereby activity of the receptor is regulated. In one embodiment, n is 1 to 4.

[0077] In one embodiment of the present invention, the autoinducer receptor is found in a gram negative or gram positive bacterium, as described above, which may or may not be present within a host. In another embodiment of the present invention, the autoinducer receptor is LasR, LasI, RhlR, or RhlI of P. aeruginosa. However, other suitable receptors include, but are not limited to, LuxR and Luxl (of V fischer), and all LuxR and LuxI homologs (e. g. , LuxP and LuxQ) (see, e. g., Miller et al., Annu. Rev. Microbiol. 55: 165-199 (2001); Dunny et al. , "Cell-toCell Signaling in Bacteria,"American Socieyfof Nlicrobiology, Washington D. C. (1999), which are hereby incorporated by reference in their entirety). As used herein, regulating may comprise activating the receptor or inhibiting the receptor.

[0078] A further aspect of the present invention relates to a method of regulating biofilm formation. This method involves contacting a cell of an organism with an autoinducer analog as described above with regard to the method for regulating an autoinducer receptor, whereby biofilm formation on the cell is regulated. In one embodiment of the present invention, biofilm formation is inhibited. In another embodiment of the present invention, biofilm architecture is modified by contact with the autoinducer analog. As described above, the biofilm is a complex, polysaccharide-laden microniche that protects the bacterial from both antibiotics and the host's humoral and cell-mediated responses. Thus,

the inhibition of biofilm formation or the alteration of the biofilm may result in less infectious bacterial strains which would be easier targets for antibiotics and host defenses.

[0079] The present invention also relates to a method for regulating the growth or virulence of an organism in a subject. This method involves contacting a cell of an organism with an autoinducer analog as described above with regard to the method for regulating an autoinducer receptor, whereby growth or virulence of the organism is regulated. As described above, the autoinducer analog of the present invention can be used to enhance organism (e. g. , bacterial) growth by acting in concert with or instead of the wild-type autoinducer (i. e. , as an autoinducer agonist) or to inhibit organism (e. g. , bacterial) growth by competing with the wild-type autoinducer (i. e. , as an autoinducer antagonist). In addition, the autoinducer analog of the present invention can inhibit virulence through inhibition of the interaction between secreted quorum sensing molecules (autoinducers) of an organism and their cognate transcriptional regulator proteins, thereby inhibiting the production of virulence factors (see Figure 1 and the description in the Examples below).

[0080] Another aspect of the present invention relates to a method of inhibiting a quorum sensing mechanism in an organism. This method involves contacting a cell of an organism possessing a quorum sensing mechanism with an autoinducer analog as described above with regard to the method for regulating an autoinducer receptor, whereby the quorum sensing mechanism of the organism is inhibited.

[0081] The aforementioned autoinducer analogs and compositions are also useful in treating an infection in a subject caused by an organism possessing a quorum sensing mechanism by administering an effective amount of an autoinducer analog or composition including an autoinducer analog to the subject.

The autoinducer analog or composition can, for example, prevent the transcriptional activation of virulence factors, such as Elastase A, Elastase B, exotoxin A, pyoverdine, pyocyanin, and other virulence factors, such as those described in PCT International Publication No. WO 01/85664, which is hereby incorporated by reference in its entirety. In addition, the autoinducer analog or

composition can, for example, inhibit biofilm formation or alter biofilm architecture for an organism possessing the quorum sensing mechanism and/or kill the organism possessing the quorum sensing mechanism by, for example, a NO- mediated suicidal mechanism. Thus, this method of the present invention is useful for treating any subject capable of being infected by an organism possessing a quorum sensing mechanism, such as mammals and plants, and, in particular, humans. The autoinducer analog can be administered alone, or in combination with suitable pharmaceutical carriers or diluents. The diluent or carrier ingredients should be selected so that they do not diminish the therapeutic effects of the autoinducer analogs or compositions. Suitable pharmaceutical compositions include those which include a pharmaceutical carrier and, for example, one or more of an autoinducer analog, as described herein.

[0082] The autoinducer analogs and compositions herein can be made up in any suitable form appropriate for the desired use ; e. g. , oral, parenteral, or topical administration. Examples of parenteral administration are intraventricular, intracerebral, intramuscular, intravenous, intraperitoneal, rectal, and subcutaneous administration. The preferred route for administration is intravenous. In cases where the autoinducer analogs or compositions are administered topically or parenterally, it is preferred that they be pre-hydrolyzed.

[0083] Suitable dosage forms for oral use include tablets, dispersible powders, granules, capsules, suspensions, syrups, and elixirs. Inert diluents and carriers for tablets include, for example, calcium carbonate, sodium carbonate, lactose, and talc. Tablets may also contain granulating and disintegrating agents, such as starch and alginic acid; binding agents, such as starch, gelatin, and acacia; and lubricating agents, such as magnesium stearate, stearic acid, and talc. Tablets may be uncoated or may be coated by known techniques to delay disintegration and absorption. Inert diluents and carriers which may be used in capsules include, for example, calcium carbonate, calcium phosphate, and kaolin. Suspensions, syrups, and elixirs may contain conventional excipients, such as methyl cellulose, tragacanth, sodium alginate; wetting agents, such as lecithin and polyoxyethylene stearate; and preservatives, such as ethyl-p-hydroxybenzoate. Dosage forms suitable for parenteral administration include solutions, suspensions, dispersions,

emulsions, and the like. They may also be manufactured in the form of sterile solid compositions which can be dissolved or suspended in sterile injectable medium immediately before use. They may contain suspending or dispersing agents known in the art.

[0084] For oral administration either solid or fluid unit dosage forms can be prepared. For preparing solid compositions, such as tablets, a suitable autoinducer analog or composition, as disclosed above, is mixed with conventional ingredients, such as talc, magnesium stearate, dicalcium phosphate, magnesium aluminum silicate, calcium sulfate, starch, lactose, acacia methylcellulose, and functionally similar materials as pharmaceutical diluents or carriers. Capsules are prepared by mixing the disclosed autoinducer analogs or compositions with an inert pharmaceutical diluent and filling the mixture into a hard gelatin capsule of appropriate size. Soft gelatin capsules are prepared by machine encapsulation of a slurry of the autoinducer analog or composition with an acceptable vegetable oil, light liquid petrolatum, or other inert oil.

[0085] Fluid unit dosage forms for oral administration such as syrups, elixirs, and suspensions can be prepared. The water-soluble forms can be dissolved in an aqueous vehicle together with sugar, aromatic flavoring agents, and preservatives to form a syrup. An elixir is prepared by using a hydro- alcoholic (ethanol) vehicle with suitable sweeteners, such as sugar and saccharin, together with an aromatic flavoring agent. Suspensions can be prepared with a syrup vehicle with the aid of a suspending agent, such as acacia, tragacanth, methylcellulose, and the like.

[0086] For parenteral administration, fluid unit dosage forms are prepared utilizing the aforementioned autoinducer analogs or compositions and a sterile vehicle, water being preferred. The autoinducer analog or composition, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions, the autoinducer analog or composition can be dissolved in water for injection and filter sterilized before filling into a suitable vial or ampule and sealing. Advantageously, adjuvants, such as a local anesthetic, preservative, and buffering agents, can be dissolved in the vehicle. To enhance the stability, the fluid unit dosage form can be frozen after

filling into the vial, and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial, and an accompanying vial of water for injection is supplied to reconstitute the liquid prior to use. Parenteral suspensions are prepared in substantially the same manner except that the autoinducer analog or composition is suspended in the vehicle instead of being dissolved, and sterilization cannot be accomplished by filtration. The autoinducer analog or composition can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the parenteral suspension to facilitate uniform distribution of the autoinducer analog or composition.

[0087] Suitable daily dosages can be based on suitable doses of antibiotics (e. g. , carbapenems). Typically, suitable daily doses are from about 10 ng/mL to about 10 mg/mL of the autoinducer analog or composition (e. g. , about 300 mg total for a human). Alternatively, the autoinducer analogs or compositions can be administered orally in foodstuffs.

[0088] Alternatively, the autoinducer analog or composition can be incorporated into a sustained release formulation and surgically implanted using conventional methods. Suitable sustained release matrices include those made of ethylene vinyl acetate and other biocompatible polymers.

[0089] For topical administration, carriers, such as phospholipid vesicles, which contain the aforementioned autoinducer analogs or composition may facilitate uptake through the skin.

[0090] The present invention is further illustrated by the following examples.

EXAMPLES Example 1--Library Synthesis [0091] In previously reported works, autoinducer analogs were synthesized individually and modified mainly on the alky side chain of AI1 (Passador et al., J. Bacteriol. 178 : 5995-6000 (1996); Kline et al., Bioorg Med.

Chem. Lett. 9: 3447-3452 (1999), which are hereby incorporated by reference in

their entirety). Although these attempts revealed the importance of the side chain for activity, no potent antagonist was discovered. On the other hand, very few investigations were made altering the HSL moiety. The only significant findings were that substitution of HSL with homocysteine thiolactone is as active as All, whereas the corresponding lactam has nearly no QS activity (Passador et al., J.

Bacteriol. 178: 5995-6000 (1996), which is hereby incorporated by reference in its entirety). Again, no potent antagonists were found.

[0092] To this end, 96 different autoinducer analogs with various substituents at the HSL position were synthesized as shown in Figure 2 and as described below.

General Synthetic Methods [0093] 3,4-dihydro-2H-pyran-2-ylmethoxymethyl polystyrene (DHP resin) was purchased from Novabiochem (Laufelfmgen, SWI). All other chemicals were purchased from Sigma-Aldrich (Milwaukee, WI) or Acros. All the solvents were anhydrous grade, or were distilled before use. All reactions were carried out in oven-dried glassware under an argon atmosphere, except the ones containing water. Anhydrous MgS04 was used as drying agent for all products. Analytical thin layer chromatography was performed on EM Reagent 0.25 mm silica gel 60- F plate. Flash chromatography was performed using EM silica gel 60 (230-400 mesh). Parallel synthesis was performed using a reactor block from Robbins Scientific Inc. (Sunnyvale, CA). Mass spectra were recorded on a Finnigan MAT95 XL spectrometer (San Jose, CA). NMR spectra were taken on a Varian Gemini 300, Inova 400, or Inova 500 MHz spectrometer (Palo Alto, CA). 1H chemical shifts were referenced to tetramethylsilane (0.00 ppm). 13C chemical shifts were referenced to CDC13 (77.0 ppm). IR spectra were recorded on a Perkin-Elmer 1760 infrared spectrometer (Shelton, CT). All (Pearson et al., Proc. Nat'l. Acad. Sci. USA 91: 197-201 (1994), which is hereby incorporated by reference in its entirety) and AI2 (Eberhard et al., Arch. Microbiol. 146: 35-40 (1986), which is hereby incorporated by reference in its entirety) were synthesized according to literature procedures.

Synthesis of the Template Molecule : Ethyl 3-oxo-12-tridecenoate (1) [0094] To a stirred solution of 10-undecenoic acid (5.055 mL, 25 mmol) in CH2C12 (50 mL) was slowly added (COC1) 2 (4.364 mL, 50 mmol). The mixture was stirred at room temperature overnight. The solvent was removed under reduced pressure to give 10-undecenoyl chloride (25 mmol). The product was used for the next reaction without further purification. 1H NMR (500 MHz, CDC13) 6 1.29-1. 48 (m, 10H), 1.71 (m, 2H), 2.05 (m, 2H), 2.88 (t, J= 7 Hz, 2H), 4.98 (m, 2H), 5.80 (m, 1H).

[0095] A stirred solution of monoethyl malonate (5. 3 g, 40 mmol) in anhydrous THF (150 mL) was cooled to-78 °C under an argon atmosphere, and n-BuLi (2.5 M, 32 mL, 80 mmol) was added drop-wise via an air-tight syringe.

After the addition, the temperature was raised to 0 °C, and the stirring was continued for 1 hour. The reaction mixture was then re-cooled to-78 °C, and 10- undecenoyl chloride (25 mmol) was added drop-wise via an air-tight syringe. The mixture was stirred for one hour at-78 °C, 30 minutes at 0 °C, and 30 minutes at room temperature. The solvent was removed under reduced pressure, and the residue was re-dissolved in 50 mL ethyl acetate. The solution was washed with 1 M HC1 (100 mL), saturated aqueous NaHCO3 (50 mL), and brine (50 mL). The organic layer was dried over MgS04 and concentrated to give (1) (6.270 g). The crude product was used for the next reaction without further purification. 1H NMR (400 MHz, CDC13) 8 1.28-1. 40 (m, 13H), 1.58 (m, 2H), 2.03 (m, 2H), 2.53 (t, J= 7 Hz, 2H), 3.43 (s, 2H), 4.21 (q, J= 7 Hz, 2H), 4.97 (m, 2H), 5.80 (m, 1H).

Ethyl 3, 3-ethylenedioxo-13-hydroxy-tridecanoate (2) [0096] The mixture of (1) (6.270 g, ca. 24.7 mmol), ethylene glycol (13.67 mL, 247 mmol) and a catalytic amount of para-toluenesulfonic acid (p-TsOH) (475 mg, 2.5 mmol) in benzene (120 mL) was refluxed at 110 °C under an argon atmosphere overnight. The solvent was removed, and the residue was diluted with 50 mL ethyl acetate. The solution was washed with saturated aqueous NaHCO3

(50 mL) and brine (50 mL), and the organic phase was dried (MgS04) and concentrated to give ethyl 3,3-ethylenedioxo-12-tridecenoate (6.973 g). The crude product was used for the next reaction without further purification. 1H NMR (400 MHz, CDC13) 8 1.26-1. 50 (m, 15H), 1.80 (m, 2H), 2.02 (m, 2H), 2.64 (s, 2H), 3.98 (m, 4H), 4.16 (q, J= 7 Hz, 2H), 4.96 (m, 2H), 5.79 (m, 1H).

[0097] A stirred solution of ethyl 3, 3-ethylenedioxo-12-tridecenoate (6.973 g, ca. 23.4 mmol) in anhydrous THF (100 mL) was cooled to 0 °C under an argon atmosphere. BH3 THF (1 M, 117 mL, 117 mmol) was added drop-wise via an air-tight syringe. The mixture was stirred at 0 °C for three hours. Then, aqueous NaHC03 (2 M, 70 mL, 140 mmol) and 30% H202 (4.375 mL, 140 mmol) were added to the reaction slowly, and the mixture was stirred at room temperature for 1.5 hours. The organic solvent was evaporated under reduced pressure, and the residue was extracted with ethyl acetate (2 x 100 mL). The combined organic layers were washed with brine, then dried over MgS04 and concentrated. The residue was purified by flash chromatography (hexane/EtOAc 2: 1, Rf= 0.21) to give (2) (3.00 g, 9.5 mmol) as a colorless liquid. 1H NMR (500 MHz, CDC13) 8 1.26-1. 40 (m, 17H), 1.56 (m, 2H), 1.79 (m, 2H), 2.65 (s, 2H), 3.63 (t, J= 7 Hz, 2H), 3.96 (m, 4H), 4.16 (q, J= 7 Hz, 2H). The overall yield of the above three steps was 38%, and 1H NMR data of all molecules were consistent with the literature data (Pearson et al., Proc. Nat'l. Acad. Sci. USA 91: 197-201 (1994), which is hereby incorporated by reference in its entirety), except for the functionalities (olefin or hydroxyl group) newly introduced into the terminus of the acyl chain.

Solid Phase Reactions [0098] DHP resin (0.98 mmol/g, 5.1 g, 5 mmol) was soaked in 50 mL CHzClz for 1 hour. Then (2) (2.3 g, 7.28 mmol) and pyridium para- toluenesulfonate (PPTS) (1.83 g, 7.29 mmol) were added. The mixture was stirred gently at room temperature for 27 hours. The resin was washed with an excess amount of CH2Ck and dried under vacuum to give (3).

[0099] The mixture of (3) (ca. 5 mmol) and LiOH-H20 (21 g, 500 mmol) in 100 mL THF/H20 (1: 1) was gently stirred and heated at 75 °C for 20 hours.

The resin was sequentially washed with an excess amount of water, THF, and CHzClz and dried under vacuum to give (4).

[0100] The parallel couplings of (4) with 96 molecules (Figure 4) were performed on a 96 well reactor block by mixing the following compounds in each well: (4) (ca. 0.05 mmol), amine/alcohol (0.5 mmol), EDC (0.5 mmol), DMAP (0.5 mmol), iPr2NEt (0.5 mmol), DMF (1.5 mL). The reactor block was assembled and rotated end-to-end at room temperature for 70 hours to afford coupling products (5).

[0101] DHP resin, the intermediates (3) and (4), and some representative coupling products (5a-d) were characterized by on-resin FT-IR (Figure 8). In each case, a small amount of dried resin (1-2 mg) was mixed with anhydrous KBr (75 mg) to make a pellet, and FT-IR spectroscopy was recorded.

DHP resin. IR (KBr) 3020,2890, 1644,1491, 1446,1237, 1060,746, 662 cni 1.

(Resin bound) Ethyl 3, 3-ethylenedioxo-13- (tetrahydropyranyl) oxy- tridecanoate (3). IR (KBr) 3459,3026, 2931,1734, 1494,1455, 1029,758, 698 cris.

(Resin bound) Lithium 3, 3-ethylenedioxo-13- (tetrahydropyranyl) oxy- tridecanoate (4). IR (KBr) 3437,3025, 2927,1603, 1494,1454, 1073,759, 699 cm-l (Resin bound) N- (13-tetrahydropyranyloxy-3, 3-ethylenedioxo-tridecanoyl) -L- homoserine lactone (5a). IR (KBr) 3372,3026, 2920,1784, 1673,1494, 1454, 1028, 756,698 cm\ (Resin bound) 2- (13-tetrahydropyranyloxy-3, 3-ethylenedioxo-tridecanoyl) - 1,3, 4-thiadiazole (5b). IR (KBr) 3450,3025, 2920,1697, 1549,1494, 1454,1032, 757,698 cm~l.

(Resin bound) 2- (13-tetrahydropyranyloxy-3, 3-ethylenedioxo-tridecanoyl) - phenol (5c). IR (KBr) 3366,3025, 2930,1682, 1533,1494, 1454,1031, 749,697 cm.

(Resin bound) 3- (13-tetrahydropyranyloxy-3, 3-ethylenedioxo-tridecanoyl) - 2, 6-dimethoxypyridine (5d). IR (KBr) 3356,3026, 2927,1685, 1592,1521, 1455,1021, 757,698 ciif [0102] After the coupling reaction, the reactor block was disassembled and connected to a vacuum line. The resin was washed with a large amount of DMF and CH2C12 to remove excessive reactants, coupling reagents, and undesired byproducts. The vacuum was applied overnight to dry the resin. 1 mL TFA/H20 (95: 5) was added into each well, and the reactor block was reassembled and rotated at room temperature for 30 minutes. The cleavage products (6) were collected into a 96 deep-well plate. 1 mL CHUCK was added to each resin to wash off the products into the collection plate. The solvents were evaporated with a water pump, and the products were dried under vacuum. Four products (6a-d, see below) were arbitrarily chosen and characterized by 1H NMR and FAB mass spectrometry to confirm the formation of the desired product.

N-(13-trifluoroacetoxy-3-oxo-tridecanoyl)-L-homoserine lactone (6a). 9.9 mg was obtained in 51% yield. 1H NMR (500 MHz, CDC13) 8 7.66 (d, J= 5 Hz, 1H), 4.6 (m, 1H), 4.48 (t, J= 8 Hz, 1H), 4.35 (t, J= 7 Hz, 1H), 4.28 (m, 1H), 3.47 (s, 2H), 2.77 (m, 1H), 2.53 (t, J= 7 Hz, 2H), 2.23 (m, 1H), 1.76 (m, 2H), 1.58 (m, 2H), 1.20-1. 40 (broad, 12H); FAB-LRMS calc'd for Cl9H29NO6F3 (MH 424.2, found 424.5.

2- (13-trifluoroacetoxy-3-oxo-tridecanamido)-1, 3, 4-thiadiazole (6b). 13.1 mg was obtained in 67% yield. 1H NMR (500 MHz, CDCl3) 8 12.76 (s, 1H), 8.82 (s, 1H), 4.33 (t, J= 7 Hz, 2H), 3.87 (s, 2H), 2.63 (t, J= 7 Hz, 2H), 1.50-1. 80 (m, 4H), 1.18-1. 22 (broad, 12H) ; FAB-LRMS calc'd for Cl7H2sN304SF3 (NUT) 424.1, found 424.4.

2- (13-trifluoroacetoxy-3-oxo-tridecanamido)-phenol (6c). 10.4 mg was obtained in 52% yield. 1H NMR (500 MHz, CDC13) 8 9.82 (s, 1H), 8.68 (s, 1H), 7.15 (m, 1H), 7.04 (m, 2H), 6.89 (m, 1H), 4.34 (t, J= 7 Hz, 2 H), 3.64 (s, 2H), 2.42 (t, J= 7 Hz, 2H), 1.76 (m, 2H), 1.56 (m, 2H), 1.20-1. 40 (broad, 12H) ; FAB- LRMS calc'd for C2lH29NOsF3 (MH+) 432.2, found 432.4.

3- (13-trifluoroacetoxy-3-oxo-tridecanamido)-2, 6-dimethoxypyridine (6d).

10.8 mg was obtained in 49% yield. 1H NMR (500 MHz, CDC13) 8 9.05 (s, 1H), 8.43 (d, J= 8 Hz, 1H), 6.29 (d, J= 8 Hz, 1H), 4.34 (t, J= 7 Hz, 2 H), 4.03 (s, 3H), 3.89 (s, 3H), 3.56 (s, 2H), 2.58 (t, J= 7 Hz, 2H), 1.74 (m, 2H), 1.59 (m, 2H), 1.20- 1.40 (broad, 12H); FAB-LRMS calc'd for C22H32N206F3 (MH+) 477.2, found 477.3.

Synthesis of 3-oxo-C12-HSL and 3-oxo-CI2-D12 3, 3-ethylenedioxydodecanoic acid [0103] A stirred solution of monoethyl malonate (1.26 g, 9.6 mmol) in 25 mL anhydrous THF was cooled to-78 °C under an argon atmosphere, and n-BuLi (2.5 M, 8 mL, 20 mmol) was added drop-wise via an air-tight syringe. After the addition, the temperature was raised to 0 °C, and the stirring was continued for 1 hour. The mixture was then re-cooled to-78 °C, and decanoyl chloride (1.044 mL, 5 mmol) was added drop-wise via an air-tight syringe. The mixture was stirred for one hour at-78 °C, 30 minutes at 0 °C and 30 minutes at room temperature. The solvent was removed under reduced pressure, and the residue was re-dissolved in 50 mL ethyl acetate. The solution was washed with 1 M HC1 (100 mL), saturated aqueous NaHC03 (50 mL), and brine (50 mL). The organic layer was dried over MgS04 and concentrated to give ethyl 3-oxododecanoate (1.456 g). The crude product was used for the next reaction. 1H NMR (500 MHz, CDC13) 6 0.88 (t, J= 7 Hz, 3H), 1.20-1. 38 (m, 12H), 1.50-1. 68 (m, 2H), 2.53 (t, J = 7.5 Hz, 2H), 3.43 (s, 2H), 4.20 (q, J= 7 Hz, 2H); 13C NMR (125 MHz, CDC13) 6 13.975, 13. 988, 22.5, 23.4, 28.91, 29.14, 29.26, 29.29, 31. 8, 42.9, 49.2, 61.2, 167.2, 202.9.

[0104] The mixture of ethyl 3-oxododecanoate (1.456 g, ca. 5 mmol), ethylene glycol (2.78 mL, 50 mmol) and a catalytic amount of p-TsOH (95 mg, 0.5 mmol) in benzene (50 mL) was heated to 110 °C under an argon atmosphere overnight. The solvent was removed, and the residue was diluted with 50 mL ethyl acetate. The solution was washed with saturated aqueous NaHCO3 (50 mL) and brine (50 mL), and the organic phase was dried over MgS04 and concentrated. The residue was purified by flash chromatography (hexane/EtOAc

8: 1, Rf= 0.21) to give ethyl 3,3-ethylenedioxydodecanoate (1.131 g, 3.95 mmol) in 79% overall yield for the above two steps NMR (500 MHz, CDC13) 8 0.88 (t, J= 7 Hz, 3EI), 1.20-1. 44 (m, 17H), 1.76-1. 82 (m, 2H), 2.65 (s, 2H), 3.94-4. 04 (m, 4H), 4.16 (q, J= 7 Hz, 2H); 13C NMR (125 MHz, CDC13) 8 14.1, 22.6, 23.4, 29. 22, 29.43, 29.48, 29.62, 31.8, 37.7, 42.5, 60.4, 65.0, 109.4, 169.5.

[0105] To a 25 mL flask were added ethyl 3,3-ethylenedioxydodecanoate (1.131 g, 3.95 mmol), LiOH. H20 (840 mg, 20 mmol), THF (5 mL), and water (5 mL). The mixture was stirred at room temperature overnight. The solution was acidified by the addition of 1 M HC1 (50 mL), and extracted with ethyl acetate (3 x 50 mL). The organic layers were combined and washed once with brine. The organic phase was dried (MgS04) and concentrated to give 3,3- ethylenedioxydodecanoic acid (0.824 g, 3.19 mmol) in 81% yield. 1H NMR (500 MHz, CDC13) 8 0.88 (t, J= 7 Hz, 3H), 1.20-1. 44 (m, 14H), 1.72-1. 80 (m, 2H), 2.72 (s, 2H), 3. 98-4. 10 (m, 4H) ; 13C NMR (125 MHz, CDC13) 8 13.9, 22.5, 23.3, 29.13, 29.35, 29.39, 29.50, 31.7, 37.5, 42.2, 64.9 (2 C), 109.2, 174.8.

[0106] All data above are consistent with the literature data reported previously (Wierenga et al., J Org. Chem. 44: 310-311 (1979); Eberhard et al., J.

Biochem. 20: 2444-2449 (1981), which are hereby incorporated by reference in their entirety).

N-(3-oxododecanoyl)-L-homoseriae lactone (3-oxo-CI2-HSL, AI-1) [0107] To a 25 mL flask were added 3,3-ethylenedioxydodecanoic acid (824 mg, 3.2 mmol), L-homoserine lactone hydrobromide (1.135 g, 6.2 mmol), EDC (1.198 g, 6.2 mmol), DMAP (0.756 g, 6.2 mmol), i-Pr2NEt (1. 1 mL, 6.3 mmol) and DMF (5 mL). The reaction was stirred at room temperature overnight.

DMF was removed under reduced pressure, and the residue was re-dissolved into 1 M HC1 (25 mL). The aqueous solution was extracted with ethyl acetate (3 x 25 mL), and the combined extracts were washed sequentially with saturated aqueous NaHCO3 and brine. The organic phase was dried (MgSO4) and concentrated to give approximately 1 g crude intermediate.

[0108] The above intermediate (1 g, ca. 2.93 mmol) was dissolved in acetone (48 mL) and water (4 mL), and p-TsOH (0.285 g, 1.5 mmol) was added.

The mixture was refluxed at 65'C for 20 hours. The acetone was removed under reduced pressure, and the residue was dissolved into 25 mL ethyl acetate. The organic layer was washed sequentially with saturated aqueous NaHCO3 and brine, then dried (MgSO4) and concentrated to give the pure product (741 mg, 2.49 mmol) in 86% yield. IR (KBr) 3295,2921, 1782,1712, 1642,1549, 1179,1017 cnfl ; 1H NMR (CDC13, 500 MHz) 8 0.88 (t, J= 7.2 Hz, 3H), 1.20-1. 38 (broad, 12H), 1.50-1. 65 (m, 2H), 2.20-2. 30 (m, 1H), 2.54 (t, J= 7.2 Hz, 2H), 2.72-2. 84 (m, 1H), 3.47 (s, 2H), 4.24-4. 32 (m, 1H), 4.48 (t, J= 9.2 Hz, 1H), 4.58-4. 64 (m, 1H), 7.67 (d, J= 5.2 Hz, 1H) ; 13C NMR (125 MHz, CDC13) 6 14.0, 22.5, 23.4, 28.89, 29.14, 29.26, 29.29, 29.43, 31.7, 43.7, 28.4, 48.9, 65.9, 166.5, 175.0, 206.3 ; EI-HRMS calc'd for C16H27O4N (M+) 297.1935, found 297.1940.

N (trans-2-hydroxycyclohexyl)-3-oxododecanamide (3-oxo-C12-HSL-D12) [0109] The same procedure for the preparation of AI-1 was used. 38 mg crude product was obtained from trans-2-aminocyclohexanol hydrochloride.

Flash chromatography (EtOAc) gave 3-oxo-Cl2-HSL-D12 in the ketone form (23 mg, 0.074 mmol, Rf= 0.29) and enol form (11 mg, 0.035 mmol, Rf= 0.17).

Ketone form : IR (KBr) 3274,2927, 1715,1652, 1617,1564 cni 1 ; 1H NMR (500 MHz, CDCl3) 6 0.88 (t, J= 7.5 Hz, 3H), 1.18-1. 40 (m, 16H), 1.58 (m, 2H), 1.72 (m, 2H), 1.90-2. 10 (m, 2H), 2.53 (t, J= 7.5, 2H), 3.35 (m, 1H), 3.43 (s, 2H), 3.67 (m, 1H), 7.19 (d, J= 5 Hz, 1H) ; 13C NMR (125 MHz, CDCl3) 8 14.1, 22.6, 23.3, 23.9, 24.5, 28.9, 29.21, 29.31, 29.34, 31.2, 31.8, 34.2, 44.0, 48.3, 55.7, 75.2, 167.3, 207.5 ; EI-HRMS calc'd for C1sH33O3N (M) 311.2455, found 311.2463.

Enol form : IR (KBr) 2921,2853, 1668,1452, 1040 cm~l ; 1H NMR (500 MHz, CDC13) 8 0.88 (t, J= 7.5 Hz, 3H), 1. 16-1. 60 (m, 18H), 1.75 (m, 2H), 2.00-2. 24 (m 4H), 3.25 (m, 1H), 3.84 (m, 1H), 5.02 (d, J= 2 Hz), 5.60 (s, 1H) ; 13C NMR (125 MHz, CDC13) # 14. 1,22. 7,23. 2,23. 4,27. 3,28. 9,29. 27,29. 33,29. 44,31. 5,31. 9, 32.2, 36.8, 54.2, 82.7, 97.4, 166.1, 167.8 ; EI-HRMS calc'd for C18H3102N (M- H20) 293.2349, found 293.2357.

Example 2-Library Screening for Agonists [0110] Screening of individual molecules for their ability to activate LasR was conducted using P. aeruginosa PAO-JP2 (lasl, rAll) harboring plasmid plash LVAgfp (a gift from Dr. Barbara Iglewski at the University of Rochester) (de Kievit et al., Appl. Environ. Microbiol. 67: 865-1873 (2001), which is hereby incorporated by reference in its entirety). Since this strain lacks the ability to produce natural AIs due to disruption of both AI synthase genes, activation of LasR relies on the addition of exogenous All. Expression of gfp (encoding green fluorescent protein) is under control of the LasR-AIl inducible lasI promoter, allowing rapid screening for LasR agonists by measuring the level of GFP expression in their presence.

GFP Reporter Assays [0111] The procedure of screening and biological assay was similar to that reported by Kline et al. (Kline et al., Bioorganic & Medicinal Chemistry Letters, 9: 3447-3452 (1999), which is hereby incorporated by reference in its entirety) and the representative process is described here. P. aeruginosa strain PAO-JP2 (last, rAll) (Rahme et al., Science 268: 1899-1902 (1995), which is hereby incorporated by reference in its entirety) harboring plasI-LVAgfp (Pearson et al., Proc. Natl Acad. Sci. USA 91: 197-201 (1994), which is hereby incorporated by reference in its entirety) was grown at 37 °C in LB with 300 llg/rnl carbenicillin. For agonist assays, an overnight culture was diluted to an OD600 of 0.1 and transferred to wells of a 96-well plate on which test compounds had previously been added and dried.

The final concentration of each analog tested was roughly 400 uM, although this is an overestimate due to purity issues. Cells were then incubated for 6 hours at 37 °C with vigorous shaking. GFP expression was detected with a Molecular Imager (BioRad) (488 nm excitation and 695 nm bandpass filter) and quantified with ImageQuant software. The OD600 of cultures was determined to normalize GFP expression to cell density.

lacZ Reporter Assays [0112] E. coli strain MG4 (pKDT37) was grown overnight in Supplemented A media with 100 llg/mL ampicillin at 30 °C and diluted 1: 100 in the same media. 1 mL of this culture was added to a test tube containing All or 3-oxo-CI2-Dl2 that had been previously aliquoted and dried. Following a 6 hour incubation at 30 °C, ß-galactosidase activity was determined using standard methods (Platt et al., Assays of the lac operon Enzymes In: Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 352-355 (1972), which is hereby incorporated by reference in its entirety).

Results [01131 Results of screening are shown in Figure 5. Three strong agonists (D11, D12, H3) and two weaker agonists (C11, El l) were present in the library.

The corresponding unconjugated amines were assayed and no agonist activity was observed, indicating the side chain is crucial for activity. Among the three strong agonists identified is homocysteine thiolactone (D11), which was included in the library as an internal control. This confirmed the reliability of the assay system as well as the assumption that the OH modification at the terminus of the acyl side chain does not interfere with agonist activity. Interestingly, Bl 1 and G12, compounds very similar to D11, were not active. Bl 1 is likely inactive due to the y-lactam ring structure (which is known to be nearly inactive (Passador et al., J.

Bacteriol. 178: 5995-6000 (1996), which is hereby incorporated by reference in its entirety) ) and interference of the oxygen substitution with hydrophobic interactions between the inducer and LasR. G12, which has the HSL amide bond replaced with an ester, is also inactive. This indicates that the amino group is required for activity in P. aeruginosa. This data is supported by the recent TraR- 3-oxo-Cg-HSL crystal structure that shows Asp70 (a residue conserved in LasR) in a hydrogen (H) bond with the HSL amino group. This putative H bond cannot be maintained by compound G12.

[0114] One of the new agonist structures identified from the library, D12, is 2-aminocyclohexanol (Figure 5B). The hydroxyl group adjacent to the amino group in D12 is clearly important for activity, since similar molecules that lack the OH group at this position, such as compounds A12 and B12, showed no activity.

Interestingly, D12 shares two structural features with All : (1) the OH group mimics the carbonyl group of HSL which participates in a hydrogen bond in the TraR crystal structure, with the highly conserved residue Trp57 ; and (2) the cyclohexane ring mimics the saturated ring structure of HSL, presumably presenting appropriate hydrophobic contacts to LasR. 3-oxo-Cl2-Dl2, containing the natural side chain, was synthesized in solution phase, and tested with both the last-gap reporter as well as a lasB-lacZ reporter in Escherichia coli. E. coli strain MG4 harboring plasmid pKDT37 expresses lasR and contains a lacZ reporter gene controlled by a LasR-AIl inducible lasB promoter (Passador et al., J.

Bacteriol. 178: 5995-6000 (1996), which is hereby incorporated by reference in its entirety). Both of these reporter genes were activated in the presence of control All or 3-oxo-Cl2-Dl2 (Figure 9), demonstrating that this agonist has LasR inducing activity comparable to the natural All. It also provides strong evidence that the agonist activity in P. aeruginosa is LasR dependent.

[0115] The second strong agonist, H3 (Figure 5A), is a quinoline derivative (Figure 5B). Comparison of H3 with other quinoline derivatives in the library such as A2, B2, and G3, suggests that the positions of N2 and the 5-exo- amine on the quinoline ring dictate activity. Since H3 shares some structural features with another autoinducer called Pseudomonas quinolone signal (PQS, Figure 1) it appears that it may exert its agonist activity via the PQS pathway.

PQS has been shown to strongly induce rhll, and mildly induce both lasR and role, but not lasI (Pesci et al., Proc. Natl Acad. Sci. USA 96: 11229-11234 (1999), which is hereby incorporated by reference in its entirety). However, these studies were done in a lasR null background and might be drastically different in the presence of LasR. A PQS analog discovered in experiments activated the lasl-gfp reporter in strain PAO-JP2 (plasl-LVAgfp), suggesting that PQS may be able to induce the lasl promoter in the presence of LasR. Other protein (s) involved in PQS signaling have not yet been identified. Two weaker agonists, Cl 1 and E11,

were also identified. Although both compounds exhibited detectable levels of agonist activity, their activities were significantly lower than the other three agonists found in the library. It should be noted that 3-oxo-C6-Cl l was not active in V fischer reporter gene assays (Schaefer et al. , J. Bacteriol. 178 : 2897-2901 (1996), which is hereby incorporated by reference in its entirety).

Example 3--Library Screening for Antagonists [0116] Each compound in the library was tested for antagonist activity using the same reporter strain, PAO-JP2 (plasI-LVAgfp) (de Kievit et al., appel.

Environ. Microbiol. 67: 865-1873 (2001), which is hereby incorporated by reference in its entirety) to find compounds that could compete against All and reduce GFP expression. The methods were the same as described in Example 2 (GFP Reporter Assay), except that 1 uM All was added to each well in combination with the library compound. The fluorescence/OD600 of each compound competing against All was reported relative to the fluorescence/OD600 of the positive control of All alone, which was set to 1. When strain PAO-JP2 (prhll-LVAgfp) was used, the positive control was 1 M All and 10 , M AI2.

Eight compounds that inhibited reporter gene expression by greater than 50% were identified (see, e. g., Figure 6).

[0117] Among these antagonists, D10, H10, C10, F10 and G9 are aniline derivatives. This set of molecules also has an ortho-or meta-substituent of a hydroxyl, carboxyamide, or pyridyl group, which can act as H bond acceptors.

The position of these substituents seems important, and depends on the particular substituent, i. e. , ortho for hydroxyl or pyridyl and ortho/meta for carboxyamide.

Structurally similar compounds differing only in the position of these substituents, E10 and G10 (Figure 6), were inactive. This indicates that the hydroxyl group in D10 and H10, the pyridyl group in G9, and the carbonyl group in C10 and F10 maintain the putative H bond interaction observed between TraR Trp57 and the 4. tumefaciens AI (Zhang et al., Nature 417: 971-974 (2002), which is hereby incorporated by reference in its entirety) by acting as H bond acceptors. The importance of the putative H bond is consistent with the observation that agonists

D11, D12, C11, and El 1 (Figure 5B) all have a potential H bond acceptor group adjacent to the amino group. It was concluded, therefore, that the combination of an aniline structure and H bond acceptor dictates the antagonist activity of this set of molecules.

Example 4--3-oxo-Ci2- (2-aminophenol) and C4- (2-amino-phenol) Analysis [0118] Since DIG (2-aminophenol) was the simplest molecule among the aniline-based antagonists, and most interestingly it differed from the strong agonist D12 only by the unsaturated ring, D10 was used for further studies. 3- oxo-C12-D10 was synthesized in solution phase, where the natural side chain was coupled with D10 and purified by chromatography. To determine if the same structural motif can be applied to inhibition of the RhlR-AI2 interaction, C4-D10 was also synthesized. Assays were performed with both the lasI-gfp reporter PAO-JP2 (plasI-LVAgfp) and an RhlR-AI2 controlled rhlI-gfp reporter, PAO-JP2 (prhlI-LVAgfp) (Figures 10A-C) (de Kievit et al., appel. Environ. Microbiol.

67: 865-1873 (2001), which is hereby incorporated by reference in its entirety).

The lasI-gfp reporter assay clearly showed a concentration dependent inhibition of GFP expression by 1 pM 3-oxo-Cl2-Dl0, resulting in approximately 60 and 90% reduction of GFP expression by the addition of 10 and 100 uM 3-oxo-Cl2-Dl0, respectively (Figure 10B). A strong reduction of GFP expression by 3-oxo-Cl2- D10 was also observed with the rhll-gfp reporter (Figure 10C). The expression induced by 1 uM All and 10 uM AI2 was inhibited by more than 80% in the presence of 50 uM 3-oxo-Cl2-D 10. These results suggested that 3-oxo-Cl2-D 10 is a strong and specific inhibitor of QS, so assays of specific QS controlled virulence factors were pursued, as discussed below. C4-D10 did not inhibit rhll-gfp expression, indicating that C4-D10 does not antagonize the RhIR-AI2 interaction (Figure 10C). This compound was also tested in virulence factor assays, but consistently showed no effect on their expression.

[0119] Tofurtherconfimtheabilityof3-oxo-Cl2-DlOtospecifically interfere with QS controlled gene expression, its ability to reduce expression of elastase, pyocyanin, and bio film, three P. aeruginosa virulence factors controlled

by QS, was tested (de Kievit et al., Sci. Med. Nov/Dec: 42-50 (1999), which is hereby incorporated by reference in its entirety). Pyocyanin, elastase, and biofilm were assayed as previously described (Smith et al. , Chem. Biol. 10: 81-89 (2003), which is hereby incorporated by reference in its entirety). Elastase activity produced by P. aerugi7losa in the presence of AIs and competing 3-OXO-Cl2-D 10 was determined by measuring breakdown of the substrate Elastin Congo Red.

Using the lasIrAlIknockout strainPAO-JP2, 10-100, uM 3-oxo-Cl2-D10 reduced elastase activity by approximately 50% when competing against 5 uM All and 10 , uM AI2 supplied exogenously (Figure 11A). This antagonist also reduced elastase activity produced by wild type P. aeruginosa strain PAO1 (Figure 11B).

These results indicate that 3-oxo-C12-D10 inhibits elastase production by P. aeruginosa, and provides additional evidence that this compound specifically interferes with las QS-controlled gene expression.

[0120] Further testing of 3-oxo-Cl2-Dl0 for its effect on pyocyanin production is presented in Figures 11 C and D. This compound was not able to interfere with pyocyanin production by either PAO-JP2 (Figure 11C) or PAOl (Figure 11D). This indicates that it is necessary to interfere with both LasR and RHIR function in order to significantly inhibit pyocyanin production.

[0121] Static biofilm assays were also performed with 3-oxo-Cl2-D10 (Figures 12A-E). In POA-JP2, formation of biofilm was dependent on the presence of exogenous AIs (Figures 12A and B). When 3-oxo-C12-D10 was added to PAO-JP2 in combination with AIs (Figure 12C), biofilm formation was slightly enhanced rather than inhibited. Similarly, 3-oxo-C12-D 10 did not interfere with biofilm formation by wild type PAO1 cells (Figures 12D and E). However, the molecule clearly had an effect on biofilm morphology, resulting in a biofilm that appeared"fluffy". Therefore, although inhibition of biofilm formation was not observed, the molecule had some impact on biofilm architecture.

Example 5--Discussion [0122] An efficient synthetic sequence to generate a library of All analogs has been developed. Additional analog libraries can be easily and rapidly

synthesized and screened for activity, based on the above description. Screening of the initial library identified new agonists that follow the same trend as previously discovered structures, allowing the conclusion that the HSL keto group and saturated carbons on the ring are involved in the interaction between the inducer and LasR. This result is consistent with the X-ray structure of a LasR homolog, TraR, that identified an H bond interaction between TraR and the HSL oxo group (Zhang et al., Nature 417: 971-974 (20002); Vannini et al., EMBO J.

21: 4393-4401 (2002), which are hereby incorporated by reference in their entirety). Several new antagonists of the QS system were also identified. It has been shown that a common structural feature for antagonists is an aniline ring with an appropriate H bond acceptor at the ortho or meta position. It is striking that the only difference between the agonist 3-oxo-Cl2-Dl2 (2- aminocyclohexanol) and the antagonist 3-OXO-CI2-DlO (2-aminophenol) is an aromatic ring. It appears that an aromatic ring somehow interferes with the ability of the antagonists to activate the R protein for DNA binding.

[0123] The antagonist 3-oxo-Cl2-D10 displays potent inhibitory activity in reporter gene assays and interferes with elastase activity and normal biofilm formation. The promoter-specific differences in inhibitory activity result from the complexity of the P. aeruginosa QS signaling network.

[0124] The potential benefits of controlling QS are very significant both medically and economically. The parallel synthesis of AI analogs allowing discovery of several new analogs that activate or inhibit QS provides a significant advancement in the understanding ofthe LasR-AIl interaction. This results in alternative therapeutic options for the treatment of chronic P. aeruginosa and other bacterial infections.

Example 6--Antagonists Found From a Focused Library Based on the Agonists [0125] A library of P. ae7*uginosa autoinducer analogs with variation targeted to the homoserine lactone (HSL) moiety was synthesized (see Examples 1-5) and a new agonist, 3-oxo-Cl2-(2-aminocyclohexanol), capable of activating LasR as a transcription factor was discovered. Two sets of focused libraries

against the quorum sensing transcription factors LasR and RhlR, respectively, were constructed. Opposing the prediction that both proteins should have the same binding site for HSL, it was determined that these two related proteins respond to different structural motifs. This suggested that the HSL binding site differs in these proteins. Subtle structural modifications to the agonists yielded compounds with antagonist activity. A series of assays was performed to show that inhibition of quorum sensing by these antagonists significantly reduced the production of virulence factors and biofilm formation.

[0126] A new structural element at the HSL position, 2- aminocyclohexanol, was found that exhibits a nearly wild type QS activity (Examples 1-4). Superimposition of the amino and hydroxyl groups in this molecule with the amino and keto groups in HSL suggested similarities in structure. Thus, it was hypothesized that a 5-or 6-membered ring with a keto or hydroxyl group (or other hydrogen bond acceptor) adjacent to the amine is sufficient for both binding and activation of LasR To test this hypothesis, the series of molecules shown in Figure 13 (compounds 1-4) was synthesized. To determine whether analogs binding to one R protein could bind other R proteins simply by substituting the cognate side chain, a second series of molecules (Figure 13,5-8) with C4 side chains was synthesized, and their ability to activate RhIR using a rhll-gfp reporter strain, PAO-JP2 (prhll-LVAgfp), was tested (de Kievit et <BR> <BR> <BR> <BR> al. , Appl. Environ. Microbiol. 67: 865-1873 (2001), which is hereby incorporated by reference in its entirety).

N-(trans-2-hydroxycyclopentyl)-3-oxododecanaaide (2) [0127] To a solution of 3, 3-ethylenedioxydodecanoic acid (55 mg, 0.213 mmol) (Pearson et al., Proc. NatlAcad. Sci. USA 91: 197-201 (1994) ; Bu et al., Synthetic agonists of a Pseudomonas aeruginosa quorum sensing molecule. submitted for publication, which are hereby incorporated by reference in their entirety) and tra7Fs-2-amirlo-cyclopentanol hydrochloride (35 mg, 0.256 mmol, purchased from Aldrich, 52,586-3) in anhydrous DMF (2 mL) was added successively EDC (49 mg, 0.256 mmol), DMAP (32 mg, 0. 256 mmol), and i- Pr2NEt (45 LL, 0.256 mmol) at room temperature. The mixture was stirred for 18

hours. After removing DMF in vacuo, the residue was dissolved into ethyl acetate (20 mL) and washed with 0.1 M HCl saturated with NaCl (20 mL). The aqueous phase was extracted with ethyl acetate (3 x 40 mL). The combined organic extracts were dried over MgS04 and concentrated to give a crude intermediate (57 mg, 79%). The 3,3-ethylenedioxy protective group was deprotected by treatment of the intermediate dissolved in 1 mL CH2C12 with 1 mL 95% TFA for 2 hours at room temperature. The organic solvent was briefly removed under reduced pressure, and the residue was mixed with saturated aqueous NaHC03 followed by extraction with ethyl acetate (3 x 40 mL). The organic layers were combined and washed once with brine, and dried over MgS04. After concentration, the residue was purified by flash chromatography on silica gel to give N-(trans-2- hydroxycyclopentyl)-3-oxododecanamide in the ketone form (14.8 mg, 0.050 mmol, Rf= 0.42) and the enol form (19.2 mg, 0.065 mmol, Rf= 0.15) in a total of 54% yield. Ketone form : IR (KBr) 3270, 2921,1716, 1646,1613, 1561 cari ;'H NMR (500 MHz, CDC13) 8 0.88 (t, J= 7 Hz, 3H), 1.20-1. 35 (m, 12H), 1.48-1. 52 (m, 1H), 1.58 (m, 2H), 1.60-1. 78 (m, 2H), 1.81 (m, 1H), 2.05 (m, 1H), 2.16 (m, 1H), 2.52 (t, J= 7 Hz, 2H, ), 3.42 (s, 2H), 3.84 (m, 1H), 3.98 (m, 1H), 4.24 (s, 1H), 7.40 (s, 1H) ; 13C (125 MHz, CDC13) : 14.1, 21.3, 22.6, 23.3, 28.95, 29.20, 29.30, 29.35, 30.3, 31.8, 32.6, 44.0, 47.9, 60.7, 79.5, 167.5, 207.4 ; EI-HRMS calc'd for C17H31O3N (M+) 297.2298, found 297.2299. Enol form: IR (KBr) 2918,2952, 1655,1424, 1365, 832 cm~l 1; 1H NMR (500 MHz, CDCl3) 8 0.88 (t, J= 7.5 Hz, 3H), 1.20-1. 38 (m, 12H), 1.52 (m, 2H), 1.62 (m, 1H), 1.78-1. 96 (m, 3H), 2.15 (td, J= 7 Hz, 1.5 Hz, 2H), 2. 30 (m, 2H), 3.56 (m, 1H, 4.27 (m, 1H), 5.03 (d, J= 1.5 Hz, 1H), 6.82 (s, 1H) ; 13C (125 MHz, CDC13) 5 14.1, 21.1, 22.6, 27.6, 28.9, 29.24, 29.29, 29.42, 30.66, 30.69, 31.8, 36.6, 57.3, 87.8, 98.2, 165.6, 168.7 ; EI-HRMS calc'd for C17H2902N (M-2O) 279.2193, found 279.2197.

N-(2-oxocyclopentyl)-3-oxododecanamide (1) [0128] Oxalyl chloride (42. 5 µL, 0.486 mmol) in 1 mL anhydrous CH2Cl2 was cooled to-78 °C under an argon atmosphere, and anhydrous DMSO (69 pal, 0.974 mmol) was added via a syringe. The mixture was stirred for 2 minutes. 2

with the 3,3-ethylenedioxy protective group at the 2-oxo position (83 mg, 0.243 mmol) in 1 mL CH2C12 was added via a syringe, and the mixture was stirred for 15 minutes. Triethylamine (169 uL, 1.215 mmol) was added to the resulting solution, and the stirring was continued for 5 minutes. After warming to room temperature, the reaction was quenched by the addition of 20 mL 0.1 M HC1. The resulting mixture was extracted with ethyl acetate (3 x 30 mL), and the combined organic layers were washed sequentially with saturated aqueous NaHCO3 and brine. All the extracts were combined, dried (MgS04), and concentrated to give the crude intermediate 1 with the protective group (57 mg). This molecule was treated with 95% TFA in the same manner as in the synthesis of 2, and its chromatographic purification on silica gel afforded 1 (42 mg, 0.14 mmol) in 85% yield. IR (KBr) 3244,2927, 1752,116, 1651,1591, 1567 ;'HNMR (500 MHz, CDC13) 8 0.879 (t, J= 7.5 Hz, 3H), 1.20-1. 36 (m, 12H), 1.52-1. 64 (m, 2H), 1.64-1. 76 (m, 1H), 1.80-1. 92 (m, 1H), 2.04-2. 16 (m, 1H), 1.98-2. 08 (m, 1H), 2.36- 2.44 (m, 1H), 2.53 (t, J= 7.5 Hz, 2H), 2.55-2. 69 (m, 1H), 4.12-4. 20 (m, 1H), 7.40 (d, J= 5 Hz, 2H); 13C (125 MHz, CDC13) 5 14.0, 18.2, 22.6, 23.3, 28.92, 29.17, 29.28, 29.31, 29.61, 31. 8, 35.0, 43.8, 48.4, 57.8, 166.0, 206.6, 214.5 ; EI-HRMS calc'd for C17H2903N (M+) 295.2142, found 295.2145.

N-(trans-2-hydroxycyclohexyl)-3-oxododecanamide (4) [0129] The same procedure as the synthesis of 1 was performed, except that trans-2-amino-cyclohexanol hydrochloride was used instead of trans-2- amino-cyclopentanol hydrochloride. Column chromatography of the crude product gave 4 in the ketone form (23 mg, 0.074 mmol, Rf= 0.29) and enol form (11 mg, 0.035 mmol, Rf= 0.17) in a total of 51% yield. Ketone form : IR (KBr) 3274,2927, 1715,1652, 1617,1564 crrfl ;'H NMR (500 MHz, CDC13) 5 0.88 (t, J = 7.5 Hz, 3H), 1.18-1. 40 (m, 16H), 1.58 (m, 2H), 1.72 (m, 2H), 1.90-2. 10 (m, 2H), 2.53 (t, J= 7.5, 2H), 3.35 (m, 1H), 3.43 (s, 2H), 3.67 (m, 1H), 7.19 (d, J= 5 Hz, 1H) ; 13C (125 MHz, CDC13) 8 14.1, 22.6, 23.3, 23.9, 24.5, 28.9, 29.21, 29. 31, 29.34, 31.2, 31. 8, 34.2, 44.0, 48.3, 55.7, 75.2, 167.3, 207.5 ; EI-HRMS calc'd for C18H3303N (M+) 311.2455, found 311.2463. Enol form : IR (KBr) 2921,2853,

1668, 1452,1040 cari ; 1H NMR (500 MHz, CDC13) 8 0.88 (t, J= 7.5 Hz, 3H), 1.16-1. 60 (m, 18H), 1.75 (m, 2H), 2.00-2. 24 (m4H), 3.25 (m, 1H), 3.84 (m, 1H), 5.02 (d, J= 2 Hz), 5.60 (s, 1H) ; 13C (125 MHz, CDC13) 8 14.1, 22.7, 23.2, 23.4, 27.3, 28.9, 29.27, 29.33, 29.44, 31.5, 31.9, 32.2, 36. 8, 54.2, 82.7, 97.4, 166.1, 167.8 ; EI-HRMS calc'd for C18H3, 02N (M-H20) 293.2349, found 293.2357.

N-(2-oxocyclohexyl)-3-oxododecanamide (3) [0130] The same procedure for the preparation of 1 was used, except that the starting material was 4 with the 3,3-ethylenedioxy protective group at the 2- oxo position (117 mg, 0.38 mmol). The crude product was purified by flash chromatography (hexane/EtOAc 1: 2, Rf= 0.38) to give 3 (87 mg, 0.28 mmol) in 73% yield. IR (KBr) 3280,2935, 1712,1654, 1628, 1550 cm~l ; 1H NMR (500 MHz, CDC13) 8 0.877 (t, J= 7 Hz, 3H), 1.20-1. 34 (m, 12H), 1.36-1. 44 (m, 1H), 1.55-1. 70 (m, 3H), 1.76-1. 84 (m, 1H), 1.84-1. 95 (m, 1H), 2.11-2. 20 (m, 1H), 2.36- 2.44 (m, 1H), 2.50-2. 60 (m, 3H), 2.60-2. 65 (m, 1H), 3.41 (s, 2H), 4.45-4. 55 (m, 1H), 7.53 (d, J= 4 Hz, 1H) ; 13C (125 MHz, CDC13) 8 14.0, 22.6, 23.3, 24.0, 27.9, 28.96, 29.20, 29.31, 29.34, 31.8, 35.1, 41.1, 43.7, 49.2, 58.2, 165.3, 206.0, 207.0 ; EI-HRMS calc'd for Cl8H3lO3N (M) 309.2298, found 309.2284.

N-(trans-2-hydroxy) cyclopentylbutanamide (6) and N (trans-2- hydroxy)cyclohexylbutanamide (8) [0131] The procedure for the preparation of C4-HSL was used for this series of molecules. The EDC-mediated coupling of butyric acid and trans-2- amino-cyclopentanol or trans-2-amino-cyclohexanol afforded 6 in 66% yield or 8 in 82% yield. 6: IR (KBr) 3284,2960, 1643,1567, 1058 cm-1; 1H NMR (500 MHz, CDC13) 8 0.952 (t, J= 7.5 Hz, 3H), 1.40-1. 50 (m, 1H), 1.60-1. 74 (m, 4H), 1.75-1. 82 (m, 1H), 1.96-2. 08 (m, 1H), 2.08-2. 18 (m, 1H), 2.19 (t, J= 7 Hz, 2H), 3.80-3. 86 (m, 1H), 3.92-4. 00 (m, 1H), 4.90 (s, 1H), 6.28 (s, 1H); 13C (125 MHz, CDC13) 8 13.6, 19.0, 21.1, 30. 0,32. 3, 38. 0,60. 5,79. 5,175. 3; EI-HRMS calc'd for C9H17O2N (M+) 171.1254, found 171.1257. 8: IR (KBr) 3294,2930, 1638, 1567, 1086,1072 cm~l ; 1H NMR (500 MHz, CDC13) : 0.952 (t, J= 7.5 Hz, 3H), 1.15-

1.20 (m, 4H), 1.60-1. 80 (m, 4H), 1.98 (m, 2H), 2.20 (t, J= 7.5, 2H), 3. 30-3.34 (m, 1H), 3.54-3. 60 (m, 1H), 4.20 (s, 1H), 6.30 (d, J= 7 Hz, 1H) ; 13C (125 MHz, CDCl3) # 13.5, 19.1, 24.0, 24.4, 31.3, 34.3, 38. 4,55. 6,74. 8,174. 9; EI-HRMS calc'd for CloHl902N (M) 185.1410, found 185.1410.

N-2-oxocyclopentylbutanamide (5) and N-2-oxocyclohexylbutanamide (7) [0132] Swern oxidation, similar to the synthesis of 1, was performed to afford 5 in 60% yield and 7 in 48% yield. 5: IR (KBr) 3256,2963, 1744,1641, 1555 cm~l ; 1H NMR (500 MHz, CDCl3) # 0.954 (t, J= 7.5 Hz, 3H), 1.56-1. 70 (m, 3H), 1.82-1. 94 (m, 1H), 2.02-2. 10 (m, 1H), 2.18-2. 26 (m, 3H), 2. 38-2. 44 (m, 1H), 2.60-2. 68 (m, 1H), 4.10-4. 20 (m, 1H), 6.00 (s, 1H); 13C (125 MHz, CDC13) 6 13. 6, 18.0, 18.9, 30.1, 34.9, 38.1, 173.3, 215.4 EI-HRMS calc'd for C9Hl5O2N (M") 169.1097, found 169.1101. 7: IR (KBr) 3295,2943, 1719,1643, 1552 cm-1; 1H NMR (500 MHz, CDC13) 6 0.95 (t, J= 7 Hz, 3H), 1.25-1. 40 (m, 1H), 1.60-1. 70 (m, 3H), 1.78-1. 90 (m, 2H), 2.10-2. 19 (m, 1H), 2.20 (t, J= 7 Hz, 2H), 2.36-2. 44 (m, 1H), 2. 50-2. 55 (m, 1H), 2.64-2. 72 (m, 1H), 4.40-4. 60 (m, 1H), 6.42 (s, 1H) ; l3C (125 MHz, CDC13) 8 13. 7,19. 1,24. 0,28. 1,35. 6, 38. 6,41. 2,58. 0,172. 7, 208.0 ; EI-HRMS calc'd for CloHl702N (M+) 183.1254, found 183.1259.

Example 7--Reporter Gene Assays [0133] Reporter strains were grown overnight in LB plus 300 llg/ml carbenicillin at 37°C and diluted to an OD600 of 0.1. Following an incubation of 1 hour, 200 ul of cell culture was added to individual wells of a 96 well plate containing appropriate amount of test compound (s). Plates were incubated for 6 hours and then scanned for fluorescence emission with a Molecular Imager (488nm excitation and 695nm bandpass filter). Fluorescence was quantified with ImageQuant software.

[0134] Using the PAO-JP2 (plasI-LVAgfp reporter strain, activity of the c-oxo-C12 molecules, All, and 1-4 was tested. Strong QS induction by AI1 was evidenced by the high GFP signal observed at as low as 1 uM of All (Figures

14A and B, All). The previously identified agonist, 3-oxo-C12 (2- aminocyclohexanol) 4, showed strong agonist activity with GFP induction only 2- fold less than All at 1, uM, and comparable to All at higher concentrations (Figures 14A and B, 4). 3-oxo-Cl2-(2-aminocyclopentanone) 1 is structurally more similar to AI1 than 4, since the oxygen of HSL was simply replaced with carbon in 1. Hence, it was expected that 1 would have strong agonist activity.

Contrary to this prediction, 1 was able to induce the reporter only at a very high concentration (Figures 14A and B, 1). Even more surprising, compounds 2 and 3 were nearly unable to induce the reporter, even at high concentrations (Figures 14A and B, 2 and 3).

[0135] The C4 compounds (A12 and 5-8) were analyzed for agonist activity using PAO-JP2 (prAlI-LVAgfp) (Figures 14C and D). Induction of GFP expression in this reporter requires addition of both All and AI2 The AI2 control and agonist assays, therefore, were performed in the presence of 1 , M AI1 and increasing concentrations of AI2 or analog (Figures 14C and D). Both ketone derivatives, 5 and 7, showed strong agonist activity, inducing the reporter at just 10 uM (Figures 14C and D, 5 and 7). In contrast, the 2-aminocyclopentanol derivative 6 had no agonist activity. Moreover, the 2-aminocyclohexanol derivative 8 had very little AI2 agonist activity; it could induce only moderate GFP expression at 400 uM (Figures 14C and D, column 8).

[0136] As mentioned above, it was initially assumed that since 2- aminocyclopentanone (1 and 5) and 2-aminocyclohexanone (3 and 7) have a keto group, they would better mimic the HSL structure than the 2-aminocyclopentanol (2 and 6) and 2-aminocyclohexanol (4 and 8). In fact, in the rhl circuit the ketone derivates were better agonists of AI2 than the alcohol derivatives. This is also consistent with the crystal structure of the TraR-3-oxo-C8 HSL complex which shows the ketone participating in an H bond. Presumably, an alcohol substitution could maintain the H bond, but less efficient binding would be expected. The results for the AI1 analogs, however, do not fit this pattern. In the las circuit, 2- aminocyclohexanol 4 was the strongest agonist, 2-aminocyclopentanone 1 was a weak agonist, and 2-aminocyclohexanone 3 had no agonist activity. Thus, based

on these results, it is proposed that the microenvironment of the protein-HSL interface differs between LasR, RhIR, and TraR.

[0137] An intriguing observation was that a subtle structural change from hydroxyl to keto group on the cyclohexane ring (4 X 3), or from keto to hydroxyl group on the cyclopentane ring (1 o 2 or 5 o 6), drastically reduced agonist activity. It is unlikely that such a small structural difference would completely eliminate binding to the respective R protein. A more likely, alternative explanation is that the cyclopentanol (2) and cyclohexanone (3) compounds can still bind LasR with similar affinity but are unable to activate it. Therefore, it appears that compounds 2 and 3 might act as All antagonists. Similarly, compound 6 should maintain the ability to bind RhlR, and therefore should act as an AI2 antagonist.

Example 8--Antagonist Assays [0138] Based on the above hypothesis, a competition assay of 2 and 3 against All, using strain PAO-JP2 (plasl-LVAgfp), was performed. As predicted, compound 2 inhibited GFP expression approximately 70% at a 100-fold excess concentration over All (Figure 15A), and compound 3 had a lesser inhibitory effect (35% reduction) under the same conditions. It should be noted that this inhibition experiment was performed with competition against 1 uM AI1, a concentration typically produced by wild type P. aeruginosa (Pearson et al., Proc.

Natl Acad. Sci. USA 92: 1490-1494 (1995), which is hereby incorporated by reference in its entirety). These results indicate that both molecules bind LasR and prevent it from being activated by All. This view is supported by the compounds'inability to inhibit GFP expression when the lasl promoter is replaced with a lac promoter in strain PAO-JP2 (pTdK-GFP) (de Kievit et al., , 4ppL Environ. MicrobioL 67: 1865-1873 (2001), which is hereby incorporated by reference in its entirety). It should also be noted that cell growth was not affected by the addition of 2 or 3 at concentrations up to 400 uM. Thus, it appears that compounds 2 and 3 act as specific inhibitors of QS controlled promoters.

Compound 6 was also tested for its ability to compete against AI2, but no inhibition of the rhll-gfp reporter gene was seen.

[0139] Next the ability of 2 and 3 to inhibit the role-gap reporter strain PAO-JP2 (prhlI-LVAgfp) was tested, since this expression is also AIl-dependent.

The Iglewski group recently reported that the rhlIpromoter is induced primarily by the LasR-AI1 complex (de Kievit et al., FEMSMicrobiol. Lett. 212: 101-106 (2002), which is hereby incorporated by reference in its entirety), but no induction of this promoter was seen with the addition of only All. It is possible that the roll-lacez reporter used in their study is more sensitive than the rhlI-gfp construct used in this study, and the small amount of GFP induced by All is below this limit of detection. However, since both All and AI2 are required to induce GFP from the PAO-JP2 (prklI-LVAghp) reporter, the data is interpreted in the context of earlier experiments that showed predominantly RhIR-AI2 activation of the rhlI promoter (Latifi et al., Mol. Microbiol. 21: 1137-1146 (1996), which is hereby incorporated by reference in its entirety). Surprisingly, compound 2 showed no antagonist activity in this assay (Figure 15B). More promising results were obtained from the assay of compound 3, where GFP expression of the roll-gap reporter was inhibited by greater than 60% at 50 uM when competing against 1 aM All and 10 uM AI2 (Figure 15B).

[0140] Inhibition by compound 3 of the rhll-gfp reporter was greater than that seen with the lasl g fp reporter. Results from the lasl-gfp assay indicate that 3 inhibits LasR-AIl dependent activation of transcription (Figure 15A). Since the rhlR promoter is also activated by LasR-AIl, inhibition of rhlI-gfp in the presence of 3 is likely due to inhibition of LasR-AI1 dependent activation of rAlR expression. This then translates into reduced activation of RhlR-AI2 dependent rAlI-gfp expression. It is also possible, however, that 3 directly interacts with RhlR and prevents AI2 binding, since All is able to act as an antagonist against RBIR and prevent binding by AI2 (Pesci et al., J. Bacteriol. 179: 3127-3132 (1997), which is hereby incorporated by reference in its entirety). The AI2 analog with the same ring structure as compound 3 (compound 7,2- aminocyclohexanone) is a strong agonist of RhlR. This supports the speculation that 3 could enter the AI binding site of RhIR and antagonize the RhIR-AI2

interaction. The chain length of RhlR autoinducers appears to be the determining factor regulating RhlR activation, since C4HSL activates RhIR but 3-oxo-C12HSL antagonizes it, and no C4 analogs have been identified that can antagonize it.

Regardless of the mechanism of inhibition by compound 3, since the rhll target is further downstream in the LasR signaling pathway than lasl, a stronger inhibition of rhll than lasl would be expected.

Example 9--Virulence Factor Assays [0141] In order to provide further evidence that compounds 2 and 3 are disrupting QS, their ability to reduce expression of virulence factors in both the AI synthase knockout (PAO-JP2) and wild type (PAO1) P. aeruginosa strains was tested. Two important virulence factors in Pseudomonas infections were chosen for the assay. First, the pigment pyocyanin that is required for disease in the Arabidopsis thaliana (Rahme et al., Proc. Natl Acad. Sci. USA 94: 13245-13250 (1997), which is hereby incorporated by reference in its entirety), Caenorhabditis elegant (Mahajan-Miklos et al., Cell 96 : 47-56 (1999), which is hereby incorporated by reference in its entirety), and Galleria mellonella (Jander et al., J.

Bactes°iol. 182 : 3843-3845 (2000), which is hereby incorporated by reference in its entirety) infection models, and causes serious tissue damage in chronic lung infections (Wilson et al., Infect. Immu7Z. 56: 2515-2517 (1988), which is hereby incorporated by reference in its entirety). Second, the metalloprotease Elastase B, which degrades immune components and causes tissue damage (Kon et al., FEMS Immunol. Med. Microbial. 25: 313-321 (1999), which is hereby incorporated by reference in its entirety).

[0142] For the pyocyanin assay, cells were grown overnight in LB then washed in fresh media and diluted to an OD600 of 0.05. This culture was grown for 3-4 hours to mid-log phase (OD600 of 0.3-0. 5), then diluted to an OD600 of 0.05, and aliquoted to test tubes containing an appropriate amount of test compound (s). Following 18 hours of growth, pyocyanin was extracted from filtered culture supernatants and quantified using standard methods (Essar et al., J.

Bacteriol. 172: 884-900 (1990), which is hereby incorporated by reference in its entirety).

[0143] Strong induction of pyocyanin in P. aeruginosa PAO-JP2 requires addition of both All and AI2 (Figures 16A and 4B). When compound 3 was added in combination with exogenous AIs, there was nearly complete inhibition of pyocyanin expression (Figures 16A and B). A very similar inhibitory effect by 3 was observed when wild type P. aeruginosa (PAO1) was assayed (Figure 16C).

The concentration dependent inhibitory effect of 3 led us to believe that 3 is specifically competing against Al activation of pyocyanin expression. Compound 2, on the other hand, reduced pyocyanin expression by 50 % in PAO-JP2 (Figures 16A and B), but the inhibition was not concentration dependent over the range tested. Moreover, 2 had no significant effect on PAO1 (Figure 16C). Therefore, the inhibitory effect of 2 on pyocyanin expression is not as significant as that of 3.

[0144] For the elastase B assay, cells (PAO-JP2) were grown overnight in PTSB media (5% Peptone, 0. 1% Tryptic Soy Broth) at 37°C, washed and diluted to an OD600 of 0.05, grown to mid-log phase, washed again, and re-suspended to an OD600 of 0.05. The culture was then added to test tubes containing test compound (s) and grown for 6 hours. Elastase B activity was quantified by incubation of 100, ul of filtered culture supernatant with 5 mg Elastin Congo Red substrate and 1 ml of 10 mM Tris pH 7.2, 1 mM CaCl2 for 6 hours at 37 °C with agitation. Elastase B activity is represented by the OD49s of the enzyme assay following reaction quenching with EDTA and centrifugation to remove unreacted substrate, divided by the OD600 of the cell culture. The same assay was run with strain PAO-1, however no exogenous autoinducers were added. Thus, a colorimetric assay was conducted using Elastin Congo Red substrate, so the observed protease activity is predominantly due to Elastase B and not other proteases that might be present in culture supernatants (Figures 16D and E) (Caballero et al., AnalyticalBiochem. 290: 330-337 (2001), which is hereby incorporated by reference in its entirety). The control experiment showed that 5 uM All and 10 uM AI2 were required for consistent induction of elastase B activity in PAO-JP2. At 50 uM 3, elastase activity was reduced to 60% of the positive control level (Figure 16D). When the same assay was perfonned on wild type P. aeruginosa, elastase activity was reduced to 30% of the control level in the presence of 100 uM 3 (Figure 16E). These results indicate that 3 likely inhibits

elastase B expression, although the inhibition was not as strong as that observed for pyocyanin expression. On the other hand, compound 2 had no significant effect on elastase activity produced by either strain (Figures 16D and E). Since 2 showed weaker inhibition of pyocyanin and elastase production than 3, future studies focused solely on 3.

Example 10--Static Biofilm Assay [0145] There is evidence that the chronic nature of P. aeruginosa infections is due in part to biofilm growth (Singh et al. , Nature 407: 762-764 (2000); Drenkard et al., Nature 416: 740-743 (2002), which are hereby incorporated by reference in their entirety). Biofilm is an attached colony of bacteria protected from biocide treatment and the host immune response by a secreted polysaccharide matrix (O'Toole et al., Annu. Rev. Microbiol. 54: 49-79 (2000), which is hereby incorporated by reference in its entirety). Its development in P. aeruginosa is QS controlled, and disruption of QS has been shown to eliminate or reduce biofilm development (Davies et al., Science 280: 295-298 <BR> <BR> <BR> <BR> (1998) ; Wu et al., Microbiol. 147: 1105-1113 (2001); Shirtliff et al. , Chez. & Biol.

9: 859-871 (2002), which are hereby incorporated by reference in their entirety).

Prevention of biofilm growth continues to be a challenge, since treatment of biofilm colonies has proven extremely difficult.

[0146] Compound 3 was tested for its effect on biofilm development in a 24-hour assay that looks at the early stages of biofilm formation. The static biofilm assay is a simple yet reliable method to monitor QS-controlled steps of biofilm formation (de Kevit et al., Appl. Environ. Microbiol. 67: 1865-1873 (2001), which is hereby incorporated by reference in its entirety). Cells (strain PAO-JP2 (pTdK-GFP) (de Kevit et al., Appl. Environ. Microbiol. 67: 1865-1873 (2001), which is hereby incorporated by reference in its entirety), which contains a constitutively expressed GFP construct for visualization by scanning confocal laser microscopy) were grown in M9 media (47.7 mM Na2HP4-7H20, 21.7 mM KH2PO4, 8. 6 rnM NaCl, 18.7 mM NH4C1, 0.5% casamino acids, 11. 1 mM glucose, 1 mM MgS04) plus carbenicillin (300 Rg/ml) overnight, washed, and re- suspended to an OD600 of 0.05, grown to mid-log phase, then diluted to an OD600

of 0.05, and added to sterile shell vials containing glass coverslips (ViroMed Laboratories, Minneapolis, Minn. ) on which the test compounds had been applied.

Cultures were grown at 37 °C with shaking for 30 minutes, and then allowed to sit at 37 °C for an additional 23.5 hours. Coverslips were then rinsed and placed on slides for visualization using an MRC1024 Laser Scanning Microscope (BioRad) with a Nikon Fluor-60X oil emersion objective lens (NA=1. 4), 488nm Argon Laser and OG515 filter.

[0147] The PAO-JP2 autoinducer synthase knockout strain did not form a biofilm unless AIs were added (Figure 17A). When this strain was grown in the presence of AIs plus 3, biofilm formation was completely inhibited (Figure 17A, right panel). The same assay was run with strain PAO-1 (pTdK-GFP), and no exogenous autoinducers were added. Inhibition of biofilm formation in the wild type strain, PAO1 (pTdK-GFP) (Figure 17B), was less obvious, but there was a noticeable difference in biofilm morphology when grown in the presence of 3.

The above results provide additional evidence that compound 3 interferes with QS signaling and reduces the production of important virulence factors.

Example 11--Discussion [0148] The studies described in Examples 1-10 yielded several new compounds that agonize or antagonize the P. aeruginosa QS circuit. Interestingly, these molecules are structurally related to each other: all compounds have a hydroxyl or keto group adjacent to the amino group that connects to the 3-oxo-C12 side chain, and also have six-or five-membered ring structures. A particularly intriguing discovery was that a very subtle change of the agonist 4 makes it an antagonist; the hydroxyl group to ketone (3), saturated ring to unsaturated benzene ring (3-oxo-Cl2-Dl0), and six-membered ring to five (2).

[0149] Despite the structural similarity of antagonists, the observed activities were different. 3-oxo-Cl2- (2-aminocyclohexanone) 3 (Figure 18), nearly completely inhibited pyocyanin and biofilm, strongly inhibited both reporter genes, and moderately inhibited elastase activity. However, 3-oxo-Cl2-D10 (Figure 18), found from the library screening, showed very good inhibition of the

reporter genes (stronger than 3) and moderate inhibition of elastase, but no inhibition of pyocyanin or biofilm. A similar result was seen with 2, which inhibited the las reporter and pyocyanin expression from PAO-JP2, but could not inhibit the rhl reporter or elastase.

[0150] It is clear that the effect of QS transcription factors is dependent on the individual promoters, due to the affinity of the promoter sequence for LasR and RhlR, and any number of other regulatory proteins influencing the level of activation of each gene. This would explain why a specific promoter would be more or less susceptible to inhibition. The difference between the capacity of each compound to inhibit specific virulence factors, however, is less clear. It is proposed that it is due to the ability of 3 to antagonize both LasR-AIl and RhlR- AI2. 3-oxo-Cz2-D10 and 2, in contrast, can only disrupt the LasR-AIl interaction.

Therefore, the rAzl regulon can still be activated even though the las regulon is significantly inhibited, resulting in induction of virulence factors. Evidence supporting this hypothesis is provided by agonist assays of AI2 analogs with the corresponding ring structures. AI2, C4-HSL, is the activator of RhlR. AI1, 3-oxo- C12-HSL, was able to competitively inhibit AI2 binding to RhlR in E. coli. AI1 reduced binding of AI2 to RhlR by 86% when present in only 4-fold excess (Kline et al., Bioorg. Med. Chem. Lett. 9: 3447-3452 (1999), which is hereby incorporated by reference in its entirety). Apparently, the long chain-HSL binds the same site as the short chain-HSL, but does not activate RhIR. The 2-aminocylcohexanone analog also fits into the HSL binding site of both LasR and RhlR. This is supported by the finding that C4- (2-aminocylcohexanone) is a strong agonist of AI2, and 3 is a strong antagonist of All (Smith et al., Chemistry & Biology 10: 81- 89 (2003), which is hereby incorporated by reference in its entirety). It appears that 3 may also antagonize the RhlR-AI2 interaction. This is a plausible hypothesis, since this compound inhibited all the virulence factors tested. Since the genes encoding elastase, pyocyanin, and biofilm are controlled by both LasR- AI1 and RhIR-AI2, it is likely that both R-proteins need to be effected to see the strong inhibition observed in the presence of 3.

[0151] In contrast, both 3-oxo-Cl2-D10 and 2 inhibited the reporter genes but were less effective at inhibiting virulence factors. It is suspected that these

two compounds elicit their effects through LasR alone, and do not interact with RhIR. This is supported by the observation that neither C4- (2- aminocylcopentanol) (Smith et al., Chemist7y & Biology 10: 81-89 (2003), which is hereby incorporated by reference in its entirety) nor C4-D10 could activate the rail-gap reporter. Since these particular HSL analogs could not activate RhIR, it seems less likely that the same analog with a 3-oxo-C12 side chain could enter the RHIR HSL binding site.

[0152] Additional support for this hypothesis that compound 3-oxo-Cl2- D 10 can inhibit LasR but not RhIR function is provided by biofilm studies of P. aeruginosa lasl deletion strains. Davies et al. and Sauer et al. have shown that lasI mutant P. aeruginosa are still able to form a biofilm but maturation into the proper biofilm morphology is altered (Wilson et al., Effect. Immun. 56: 2515-2517 (1988); Sauer et al., J. Bacteriol. 184: 1140-1154 (2002), which are hereby incorporated by reference in their entirety). It is believed that compound D10 similarly disrupts normal biofilm development, although it cannot completely inhibit biofilm formation.

Example 12--Determination of the Chirality of A1 and Compounds 2-4 [0153] The agonist 4 and antagonists 2 and 3 (Figure 18) were synthesized in racemic mixtures. Although HSL in All is known to be the natural L-form based on the consideration of its biosynthetic mechanism, there appears to be no report describing that the D-form is inactive. Moreover, it is not necessarily true that the synthetic agonist and antagonists bear the same chirality to AI1 or that either chirality has greater activity. This is a particularly interesting issue for antagonists. Thus, both L-and D-forms (S-and R-configurations of the 2-amino carbon center) of each molecule were synthesized. Moreover, in 2 and 4, there are cis and trans geometry of the aminoalcohol's stereochemistry. Thus, the cis molecules were synthesized as well.

[0154] The chemistry was very straightforward. The highly enantioselective epoxidation catalysts developed by Jacobsen were used (Figure 19). The remaining procedures are known in the literature (Jacobsen, Ace. Chem.

Res. 33 (6): 421-431 (2000), which is hereby incorporated by reference in its entirety) or are described above. D-HSL was synthesized from D-homoserine, and coupled with the 3-oxo-Cl2-side chain. Reporter assays using the las reporter, PAO-JP2 (plasl-LVAgfp), can be performed as described above to determine the agonist or antagonist activity of each compound.

Example 13--Expansion of las Antagonist Focused Libraries Based on the Cyclohexane and Cyclopentane Skeleton [0155] In this example, the library of las antagonists is to be expanded based on the structure of 2 and 3. The synthesis of the library will be performed based on the previously developed solid phase approach described in Figure 2.

The hydroxyl or keto group adjacent to the amino group connecting the 3-oxo- Cl2-side chain will be kept intact and the substitutions on the ring will be modified.

[0156] To diversify the molecular structures in the library based on 3,3- aminoacyclohexene will be coupled with the resin-immobilized 3-oxo-Cl3-side chain (Figure 20A). 3-aminoacylhexene is commercially available in both R and S forms, so that the results obtained in Example 12 can be considered for the choice of molecule. Alternatively, the racemic form can be used to screen antagonist structure, and then the enantiomerically pure form can be re-synthesized by means of solution phase chemistry. The olefin will be oxidized to epoxide, which gives the blanching point material. Nucleophilic substitution of various organometallic alkyl and aryl groups or Lewis acid aided substitutions of various nucleophiles will yield various molecules in the library. Oxidation of the resulting molecules by Dess-Martin reagent will convert the corresponding ketones. The individual molecules can also be synthesized in solution phase, although such synthesis is more laborious but still feasible for smaller collections of molecules. Similarly, the same library construction can be performed for cyclopentane derivatives, starting from 3-amino-cyclopentene.

[0157] The individual molecules will be tested using the las reporter to determine the agonist or antagonist activities for the las circuit (for antagonist assays, 1 pM AI1 will be used for competition). Once hits are identified, these

molecules can then be re-synthesized in solution phase for further evaluation. The molecules will also be tested for inhibition of the rhl circuit using the rhl reporter.

Upon finding potent antagonists, they will be tested for their effect on virulence factor and biofilm production.

[0158] In addition to the competitive inhibitors described above, discovery suicide inhibitors is of interest. Therefore, the epoxide molecule as well as the chlorine-substituted ketone (Figure 20B) will also be tested.

Example 14--Expansion of the 3-oxo-Cl2 Library Based on 2-aminophenol or Other Aniline Derivatives [0159] It has been determined that 3-oxo-Cl2-D10 is more potent than 3 for las circuit inhibition. It has also been determined from the library screening that some other substituted aniline compounds possibly antagonize the las circuit.

Therefore, the library will be expanded with various anilines, particularly ortho- and meta-substituted anilines (Figure 21A). Also, expansion of ring size will be examined (Figure 21B).

[0160] Because a number of anilines are commercially available, the library can be initially constructed from the commercial molecules. Again, either solid phase as in Figure 2 or solution phase using a solid-immobilized dicarbodiimide reagent to carry on the coupling can be used. The remaining procedure is the same as described above.

Example 15--Synthesis and Screening of Libraries for the rhl Quorum Sensing Circuit [0161] Although the las circuit is the master regulatory system for QS, the above results indicate that the inhibition of both circuits may be critical to shut down the entire QS system to see the same effectiveness as the genetic knockout QS strains. This working hypothesis leads to the construction of a library for the rlzl circuit, i. e. C4-side chain library.

[0162] Unfortunately, the molecules in the C4-focused library constructed in Figure 13 did not antagonize the rhl QS circuit. However, the studies shown in

Figure 14 indicated that the microenvironment in the autoinducer binding sites of LasR and RhIR is slightly different. This finding in turn indicates that the structural elements that antagonize LasR may not be able to antagonize RhIR strongly. Thus it is necessary to construct C4-libraries and screen for rA/ antagonists. To this end, a C4-library similar to the original 3-oxo-Cl2-library (Figure 4) has been constructed. The synthesis of such a library is accomplished by coupling various amines with butyric chloride under aqueous basic conditions or anhydrous DMAP-Et3N conditions.

[0163] High throughput screening can be carried out for agonists and antagonists using the rAI reporter. For the antagonist assay, 1, uM AI1 and 10 uM AI2 can be used to compete for the rAI-controlled GFP expression, since this concentration likely mimics well the wild-type situation.

[0164] Once hits for antagonist activity from the library are identified, the double antagonist competing conditions using 3-oxo-Cl2-D10 and the C4- antagonist in the presence of All and AI2 can be used. The ability to attenuate pathogenicity and biofilm formation will be further evaluated with a combination of antagonists of both circuits.

[0165] Although preferred embodiments have been depicted and described in detail herein, it will be apparent to those skilled in the relevant art that various modifications, additions, substitutions, and the like can be made without departing from the spirit of the invention and these are therefore considered to be within the scope of the invention as defined in the claims which follow.