Title:
COMBINED LIGAND, AS WELL AS COMBINED BIOMIMETIC CHROMATOGRAPHY MEDIUM, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/127686
Kind Code:
A1
Abstract:
The present invention relates to a combined ligand, as well as a combined biomimetic chromatography medium, a preparation method therefor, and application thereof. Regarding the combined biomimetic chromatography medium, a hydrophilic porous microsphere is taken as a chromatography medium, activation is performed using allyl bromide, bromination alcoholization is performed using N-bromosuccinimide, and then coupling is performed with the combined ligand. A sequence of the combined ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole. The combined biomimetic chromatography medium has two functional groups, i.e., phenylalanine-tyrosine-glutamine tripeptide and aminobenzimidazole, at the same time. In the retention of the high selectivity of a polypeptide ligand to an antibody, the introduction of a hydrophobic charge induction ligand makes an elution condition milder and enables efficient antibody isolation.
Inventors:
YAO SHANJING (CN)
LIN DONGQIANG (CN)
ZHANG QILEI (CN)
ZOU XUJUN (CN)
LU HUILI (CN)
LIN DONGQIANG (CN)
ZHANG QILEI (CN)
ZOU XUJUN (CN)
LU HUILI (CN)
Application Number:
PCT/CN2018/072780
Publication Date:
July 04, 2019
Filing Date:
January 16, 2018
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C07K5/087; B01D15/08; C07K1/107; C07K1/22
Foreign References:
| CN104096544A | 2014-10-15 |
Other References:
LU , HUILI ET AL.: "Affinity Biomimetic Chromatography and its Applications for Antibody Purification", CIESC JOURNAL, vol. 67, no. 9, 30 September 2016 (2016-09-30), pages 3523 - 3535
Attorney, Agent or Firm:
HANGZHOU TIANQIN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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