Title:
COMPLEX CONTAINING PROTEIN WITH LIGAND BINDING CAPACITY, AND METHOD OF SCREENING SUBSTANCE CAPABLE OF BINDING TO PROTEIN WITH LIGAND BINDING CAPACITY
Document Type and Number:
WIPO Patent Application WO/2005/111071
Kind Code:
A1
Abstract:
A complex comprising (1) a protein with ligand binding capacity, or derivative thereof, selected from the group consisting of a protein capable of specific binding to a ligand, a fragment of the protein containing a ligand binding site, and a substance containing a polypeptide containing the above protein or fragment, and (2) a partner substance capable of specific binding to, at sites other than the ligand binding site, the protein with ligand binding capacity or derivative thereof. Further, there is provided a method of screening with the use of this complex.
Inventors:
OMI HIROYUKI (JP)
TAKEDA SHIGEKI (JP)
TAKEDA SHIGEKI (JP)
Application Number:
PCT/JP2005/008766
Publication Date:
November 24, 2005
Filing Date:
May 13, 2005
Export Citation:
Assignee:
PEPTIDE DOOR CO LTD (JP)
UNIV GUNMA NAT UNIV CORP (JP)
OMI HIROYUKI (JP)
TAKEDA SHIGEKI (JP)
UNIV GUNMA NAT UNIV CORP (JP)
OMI HIROYUKI (JP)
TAKEDA SHIGEKI (JP)
International Classes:
G01N33/50; C07K14/705; C07K19/00; C12N15/09; G01N33/15; G01N33/53; (IPC1-7): C07K14/705; C07K19/00; C12N15/09; G01N33/50; G01N33/53
Foreign References:
| JP2001524816A | 2001-12-04 | |||
| US5599681A | 1997-02-04 | |||
| JP2001513995A | 2001-09-11 |
Other References:
TAKEDA S. ET AL: "Identification of surrogate ligands for orphan G protein-coupled receptors.", LIFE SCI., vol. 74, no. 2-3, 2003, pages 367 - 377, XP002990364
TAKEDA S. ET AL: "Receptor-Galpha fusion proteins as a tool ligand screening.", LIFE SCI., vol. 68, no. 19-20, 2001, pages 2319 - 2327, XP002990365
TSAI S.C. ET AL: "Immunological and biochemical differentiation of guanyl nucleotide binding proteins: interaction of Go alpha with rhodopsin, anti-Go alpha polyclonal antibodies, and a monoclonal antibody against transducin alpha subunit and Gi alpha.", BIOCHEMISTRY., vol. 26, no. 15, 1987, pages 4728 - 4733, XP002990366
ROBAS N. ET AL: "MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion.", J.BIOL.CHEM., vol. 278, no. 45, 2003, pages 44400 - 44404, XP002306358
TAKEDA S. ET AL: "Receptor-Galpha fusion proteins as a tool ligand screening.", LIFE SCI., vol. 68, no. 19-20, 2001, pages 2319 - 2327, XP002990365
TSAI S.C. ET AL: "Immunological and biochemical differentiation of guanyl nucleotide binding proteins: interaction of Go alpha with rhodopsin, anti-Go alpha polyclonal antibodies, and a monoclonal antibody against transducin alpha subunit and Gi alpha.", BIOCHEMISTRY., vol. 26, no. 15, 1987, pages 4728 - 4733, XP002990366
ROBAS N. ET AL: "MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion.", J.BIOL.CHEM., vol. 278, no. 45, 2003, pages 44400 - 44404, XP002306358
Attorney, Agent or Firm:
Morita, Kenichi (Itabashi-chuo Bldg. 67-8, Itabashi 2-chom, Itabashi-ku Tokyo 04, JP)
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