Title:
COMPOSITION COMPRISING STEM CELL-DERIVED EXTRACELLULAR VESICLE AS ACTIVE INGREDIENT
Document Type and Number:
WIPO Patent Application WO/2023/022473
Kind Code:
A1
Abstract:
The present invention relates to a pharmaceutical composition comprising pineal gland hormone-treated mesenchymal stem cell-derived extracellular vesicles as an active ingredient. The extracellular vesicles extracted from pineal gland hormone-treated mesenchymal stem cells or a culture thereof according to the present invention are observed to have increased expression levels of miR-29b-3p, let-7a-3p, let-7b-5p, let-7c-3p, miR-153-3p, miR-26a-2-3p, and miR-486-5p, which are micro RNAs associated with anti-fibrosis and anti-inflammation. When administered into mouse models with chronic renal failure, the extracellular vesicles exhibited anti-inflammatory and anti-fibrotic effects, proliferated fibroblasts and inhibited apoptosis in kidney tissues, and recovered renal functions and thus can be advantageously used for preventing or treating inflammatory or fibrotic diseases or chronic renal failure.
Inventors:
LEE SANG HUN (KR)
Application Number:
PCT/KR2022/012195
Publication Date:
February 23, 2023
Filing Date:
August 16, 2022
Export Citation:
Assignee:
STEMBIO CO LTD (KR)
International Classes:
A61K35/28; A61K31/7105; A61P13/12; A61P29/00; A61P43/00; C12N5/0775; C12N15/10
Domestic Patent References:
| WO2017129977A1 | 2017-08-03 |
Foreign References:
| KR102222826B1 | 2021-03-05 |
Other References:
YOON YEO MIN, LEE JUN HEE, SONG KEON‐HYOUNG, NOH HYUNJIN, LEE SANG HUN: "Melatonin‐stimulated exosomes enhance the regenerative potential of chronic kidney disease‐derived mesenchymal stem/stromal cells via cellular prion proteins", JOURNAL OF PINEAL RESEARCH, MUNKSGAARD, COPENHAGEN, DK, vol. 68, no. 3, 1 April 2020 (2020-04-01), DK , XP093036131, ISSN: 0742-3098, DOI: 10.1111/jpi.12632
BIRTWISTLE LUCY, CHEN XIN-MING, POLLOCK CAROL: "Mesenchymal Stem Cell-Derived Extracellular Vesicles to the Rescue of Renal Injury", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 22, no. 12, 1 January 2021 (2021-01-01), pages 6596, XP093036129, DOI: 10.3390/ijms22126596
LV WENSHAN, FAN FAN, WANG YANGANG, GONZALEZ-FERNANDEZ EZEKIEL, WANG CHEN, YANG LILI, BOOZ GEORGE W., ROMAN RICHARD J.: "Therapeutic potential of microRNAs for the treatment of renal fibrosis and CKD", PHYSIOLOGICAL GENOMICS, AMERICAN PHYSIOLOGICAL SOCIETY, US, vol. 50, no. 1, 1 January 2018 (2018-01-01), US , pages 20 - 34, XP093036128, ISSN: 1094-8341, DOI: 10.1152/physiolgenomics.00039.2017
YEA JI-HYE, YEO MIN YOON, JUN HEE LEE, CHUL WON YUN, SANG HUN LEE : "Exosomes isolated from melatonin-stimulated mesenchymal stem cells improve kidney function by regulating inflammation and fibrosis in a chronic kidney disease mouse model", JOURNAL OF TISSUE ENGINEERING, vol. 12, 29 November 2021 (2021-11-29), XP093036127, DOI: 10.1177/20417314211059624
BIRTWISTLE LUCY, CHEN XIN-MING, POLLOCK CAROL: "Mesenchymal Stem Cell-Derived Extracellular Vesicles to the Rescue of Renal Injury", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 22, no. 12, 1 January 2021 (2021-01-01), pages 6596, XP093036129, DOI: 10.3390/ijms22126596
LV WENSHAN, FAN FAN, WANG YANGANG, GONZALEZ-FERNANDEZ EZEKIEL, WANG CHEN, YANG LILI, BOOZ GEORGE W., ROMAN RICHARD J.: "Therapeutic potential of microRNAs for the treatment of renal fibrosis and CKD", PHYSIOLOGICAL GENOMICS, AMERICAN PHYSIOLOGICAL SOCIETY, US, vol. 50, no. 1, 1 January 2018 (2018-01-01), US , pages 20 - 34, XP093036128, ISSN: 1094-8341, DOI: 10.1152/physiolgenomics.00039.2017
YEA JI-HYE, YEO MIN YOON, JUN HEE LEE, CHUL WON YUN, SANG HUN LEE : "Exosomes isolated from melatonin-stimulated mesenchymal stem cells improve kidney function by regulating inflammation and fibrosis in a chronic kidney disease mouse model", JOURNAL OF TISSUE ENGINEERING, vol. 12, 29 November 2021 (2021-11-29), XP093036127, DOI: 10.1177/20417314211059624
Attorney, Agent or Firm:
WIE, Byoung Gap (KR)
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