Title:
COMPOSITION FOR INHIBITING CANCER METASTASIS USING APOPTOTIC CELLS
Document Type and Number:
WIPO Patent Application WO/2017/135660
Kind Code:
A1
Abstract:
The present invention relates to a composition for inhibiting cancer metastasis using apoptotic cells. Particularly, when a conditioned medium was prepared by co-cultivating macrophages and the apoptotic cells, and a mouse lung adenocarcinoma cell 344SQ with EMT induced through TGF-β1 was treated with the conditioned medium, inhibition of EMT, migration, and penetration was confirmed, and inhibition of EMT, migration, and penetration in an A549 cell, which is a human non-small cell lung cancer (NSCLS) cell line, was confirmed. In addition, the same results of cancer metastasis and invasion inhibition were confirmed in various human cancer cell lines such as breast cancer, colon cancer, and prostate cancer.
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Inventors:
LEE JIHEE (KR)
KIM YONG-BAE (KR)
AHN YOUNG-HO (KR)
KIM YONG-BAE (KR)
AHN YOUNG-HO (KR)
Application Number:
PCT/KR2017/001049
Publication Date:
August 10, 2017
Filing Date:
February 01, 2017
Export Citation:
Assignee:
EWHA UNIV - IND COLLABORATION FOUND (KR)
International Classes:
A61K35/15; A23L33/10; A61K35/12
Other References:
SCHEFFER, S. R ET AL.: "Apoptotic, but Not Necrotic, Tumor Cell Vaccines Induce a Potent Immune Response In Vivo", INTERNATIONAL JOURNAL OF CANCER, vol. 103, no. 2, 26 November 2002 (2002-11-26), pages 205 - 211, XP055405789, Retrieved from the Internet
WEIGERT, A. ET AL.: "Apoptotic Cells Promote Macrophage Survival by Releasing the Antiapoptotic Mediator Sphingosine-1-phosphate", BLOOD, vol. 108, no. 5, 2006, pages 1635 - 1642, XP055405791, Retrieved from the Internet
CHOI, J. -J. ET AL.: "The Role of Macrophages in the In Vitro Generation of Extracellular DNA from Apoptotic and Necrotic Cells", IMMUNOLOGY, vol. 115, no. 1, May 2005 (2005-05-01), pages 55 - 62, XP055543908, Retrieved from the Internet
ZHOU. N. ET AL.: "Exposure of Tumor-associated Macrophages to Apoptotic MCF-7 Cells Promotes Breast Cancer Growth and Metastasis", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 16, no. 6, 26 May 2015 (2015-05-26), pages 11966 - 11982, XP055405795, Retrieved from the Internet
FADOK, V. A. ET AL.: "Macrophages that Have Ingested Apoptotic Cells In Vitro Inhibit Proinflammatory Cytokine Production through Autocrine/paracrine Mechanisms Involving TGF-beta, PGE2, and PAF", JOURNAL OF CLINICAL INVESTIGATION, vol. 101, no. 4, 15 February 1998 (1998-02-15), pages 890 - 898, XP055540343, Retrieved from the Internet
WEIGERT, A. ET AL.: "Apoptotic Cells Promote Macrophage Survival by Releasing the Antiapoptotic Mediator Sphingosine-1-phosphate", BLOOD, vol. 108, no. 5, 2006, pages 1635 - 1642, XP055405791, Retrieved from the Internet
CHOI, J. -J. ET AL.: "The Role of Macrophages in the In Vitro Generation of Extracellular DNA from Apoptotic and Necrotic Cells", IMMUNOLOGY, vol. 115, no. 1, May 2005 (2005-05-01), pages 55 - 62, XP055543908, Retrieved from the Internet
ZHOU. N. ET AL.: "Exposure of Tumor-associated Macrophages to Apoptotic MCF-7 Cells Promotes Breast Cancer Growth and Metastasis", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 16, no. 6, 26 May 2015 (2015-05-26), pages 11966 - 11982, XP055405795, Retrieved from the Internet
FADOK, V. A. ET AL.: "Macrophages that Have Ingested Apoptotic Cells In Vitro Inhibit Proinflammatory Cytokine Production through Autocrine/paracrine Mechanisms Involving TGF-beta, PGE2, and PAF", JOURNAL OF CLINICAL INVESTIGATION, vol. 101, no. 4, 15 February 1998 (1998-02-15), pages 890 - 898, XP055540343, Retrieved from the Internet
Attorney, Agent or Firm:
LEE, Won Hee (KR)
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