A composition and a Process for preparation of Nano Bio-Nutrient Processed Organic Spray Background of the invention:

The present invention relates to field of processed organic fertilizers.

Fertilizer is any organic or inorganic material of natural or synthetic origin (other than liming materials) that is added to a soil to supply one or more plant nutrients essential to the growth of plants.

Fertilizers typically provide, in varying proportions: six macronutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S) for the growth of plants. The growth of the plant is directly proportional to the amount of nitrogen (N), phosphorus (P), potassium (K) provided to them. These fertilizers can be classified into Organic and Inorganic Fertilizers.

Organic fertilizers are naturally occurring fertilizers (e.g. compost, manure) wherein the macronutrients nitrogen (N), phosphorus (P), potassium (K) are provided from natural source. Naturally occurring organic fertilizers include manure, slurry, worm castings, peat, seaweed, humic acid, and guano.

Processed organic fertilizers include compost, blood meal, bone meal, humic acid, amino acids, and seaweed extracts. Other examples are natural enzyme-digested proteins, fishmeal, and feather meal. Decomposing crop residue (green manure) from prior years is another source of fertility.

Organic fertilizers also have the advantage of avoiding certain problems associated with the regular heavy use of artificial fertilizers. The necessity of reapplying artificial fertilizers regularly (and perhaps in increasing quantities) to maintain fertility; Extensive runoff of soluble nitrogen and phosphorus, leading to eutrophication of bodies of water are some of them. Amino acids are molecules containing an amine group, a carboxylic acid group, and a side-chain that is specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen.

The use of amino acids in the field of organic fertilizers is also known through ages. The chelating ability of amino acids has been used in fertilizers for agriculture to facilitate the delivery of minerals to plants in order to correct mineral deficiencies, deficiencies from occurring and improving the overall health of the plants.

However Organic fertilizers have certain limitations. Major limitations of organic are that they generally require large amounts to have desired effects; smaller quantities of organic fertilizers provide lesser amount of the macronutrients nitrogen (N), phosphorus (P), potassium (K); Quality for most has to be enhanced by combining with expensive mineral fertilizers.

It is therefore the object of the present invention to prepare a processed organic fertilizer for enhancing plant growth wherein the final product contains higher percentage of organic matter and so that desired effect (in the final yield) can be achieved by applying smaller quantities.

Applying smaller quantities of processed fertilizers can be achieved when the processed organic fertilizer is processed in such a manner that in the final solution obtained each nano-particulate material contains higher number of main amino acids, higher amount of macronutrients nitrogen (N), phosphorus (P), potassium (K) and higher percentage of organic matter.

Prior Art

The use of amino acids gathered either from animal organs or parts of plants to formulate organic fertilizers is a known art. Some patents like CN102040408 (China), CN101973809 (China), CN101880203 (China), CN101343201 (China) and CA 2363479 (Canada) mention about the processing of amino acids gathered either from animal organs or parts of plants to formulate organic fertilizers wherein only a small amount of main amino acids, macronutrients nitrogen (N), phosphorus (P), potassium (K) and organic matter is found and hence they are to be applied in large amount to have desired effects.

Other patents such as CN101973820 (China), CN 101913941 (China), CN 101880200 (China) and CN 101475416 (China) are all attempts wherein amino acids are gathered either from animal organs or parts of plants or in compound form and are mixed with synthetic chemicals in order to achieve the desired result.

There have been some isolated attempts such as PT103322 wherein extraction of a protein from the seeds cotyledons or plantlets of Lupinus genus has been made to formulate a growth promoter or fertilizers wherein again only a small amount of main amino acids, macronutrients nitrogen (N), phosphorus (P), potassium (K) and-organic matter is found and hence they are to be applied in large amount to have desired effects. Particular reference is made here in respect of WO200013502 wherein fertilizer compositions and methods for their use are provided. However teachings of the said patent uses more of Co Enzymes (A non-proteinaceous organic substance that usually contains a vitamin or mineral and combines with a ^" specific protein, the apoenzyme, to form an active enzyme system). Also the teachings of the said patent discusses usage of Carbohydrates such as Mono & Di Saccharides and chelating or complex agents and merely adding rather than extracting of ingredients and making a product. The said patent has limitations wherein again only a small amount of main amino acids, macronutrients nitrogen (N), phosphorus (P), potassium (K) and organic matter is found and hence they are to be applied in large amount to have desired effects. As a result more concentrate his required for less of water for application and the concentrate is applied daily or weekly or in 2 weeks or monthly depending on crop, which increases the cost of the product. Description of the Invention:

According to the present invention animal organs and/or plant parts of various types which are rich in amino acids are gathered, washed with hot water for removal of debris, dirt etc. and even helps as a sterilizing agent.

To these, water and more preferably Reverse Osmosis membrane-technology filtered (RO) water is added in a ratio between 1 :2 and 1 :8. Reverse Osmosis (RO) is a membrane-technology filtration method that removes many types of large molecules and ions from solutions by applying pressure to the solution when it is on one side of a selective membrane.

Enzymes (any of numerous proteins or conjugated proteins produced by living organisms and functioning as biochemical catalysts) mainly of plant origin and more preferable of organic origin are added in the composition. This composition is placed in a reactor and heated up to 100 degree centigrade for time between 12 to 48 hours. The obtained solution is centrifuged and a clear extract is collected. This extract is passed through filter bed or filters, which is between 0.5 and 2.5 microns thick.

The clear solution after passing through the filters is again taken in to reactor and heated till solution reduces to half (e.g. if 2 kg of solution is taken in reactor, it is heated up to 105 degree centigrade till the solution becomes 1 kg).

To the obtained concentrated solution preservatives, preferably food grade preservatives are added. It is pertinent to mention that in the present invention there is neither usage of any carbohydrates nor any chelating or complex agents. The final solution obtained is nano-particulate material (20 nanometres in size) and each nano-particulate material contains up to 17 main amino acids and up to 70% organic matter. The application of the final product can be done in any one or the combination of the following three ways: 1 ) The obtained final product is to be mixed with water wherein with every 1.25 ml of solution one liter of water is mixed and sprayed directly over the leaves of the crops. The quantity of solution is to be applied depending upon the plant species. For example in an area of 1 acre of paddy filed we require 250 ml of the spray to be mixed with 200 liters of water; 2) The obtained final product is to be mixed with water wherein with every 1.25 ml of solution one liter of water is mixed and can be applied to the roots of the plants; 3) The obtained final product is to be mixed with water wherein with every 1 .25 ml of solution one liter of water. Seeds of the corps such as paddy then can be soaked in to the diluted product and thereafter sprayed to the fields for cultivation.

As the in final product each nano-particulate material contains higher number of majn amino acids, higher amount of macronutrients nitrogen (N), phosphorus (P), potassium (K) and higher percentage of organic matter, application of the product is required for lesser frequent duration than any existing market product (10- 15 days time interval) depending on crop hence making the product more economical. This technology has shown promising results enhance plant growth on all crop varieties where farmers has more than 45% of extra yield when compared to routine cultivation procedures.