Title:
COMPOSITION FOR PRODUCING 3-HYDROXY PHLORETIN
Document Type and Number:
WIPO Patent Application WO/2019/066446
Kind Code:
A3
Abstract:
The present invention relates to a wild-type CYP102A1 mutant for producing 3-hydroxy phloretin among metabolites of phloretin, and can mass-produce 3-hydroxy phloretin by oxidizing phloretin by using, as catalysts, a point mutant of wild-type CYP102A1 which is a bacterial cytochrome P450 enzyme, CYP102A1 chimera obtained by fusing a heme domain of the point mutant of the wild-type CYP102A1 with a reductase of a natural variant of the wild-type CYP102A1, and a point mutant of the CYP102A1 chimera.
Inventors:
YUN CHUL-HO (KR)
NGUYEN NGOC ANH (KR)
LE THIEN KIM (KR)
KANG HYUNG-SIK (KR)
NGUYEN NGOC ANH (KR)
LE THIEN KIM (KR)
KANG HYUNG-SIK (KR)
Application Number:
PCT/KR2018/011350
Publication Date:
May 23, 2019
Filing Date:
September 27, 2018
Export Citation:
Assignee:
UNIV NAT CHONNAM IND FOUND (KR)
International Classes:
C12N9/02; C12P7/26
Foreign References:
| KR20170029272A | 2017-03-15 | |||
| KR101774940B1 | 2017-09-20 | |||
| KR20150001169A | 2015-01-06 |
Other References:
KANG, JI-YEON ET AL.: "Chimeric cytochromes P450 engineered by domain swapping and random mutagenesis for producing human metabolites of drugs", BIOTECHNOLOGY AND BIOENGINEERING, vol. 111, no. 7, July 2014 (2014-07-01), pages 1313 - 1322, XP055614345
EICHENBERGER, MICHAEL: "Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties", METABOLIC ENGINEERING, vol. 39, 31 October 2016 (2016-10-31), pages 80 - 89, XP029880939
VAN VUGT-LUSSENBURG, BARBARA M. A.: "Identification of critical residues in novel drug metabolizing mutants of cytochrome P450 BM3 using random mutagenesis", JOURNAL OF MEDICINAL CHEMISTRY, vol. 50, 2007, pages 455 - 461, XP002649499, DOI: doi:10.1021/jm0609061
EICHENBERGER, MICHAEL: "Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties", METABOLIC ENGINEERING, vol. 39, 31 October 2016 (2016-10-31), pages 80 - 89, XP029880939
VAN VUGT-LUSSENBURG, BARBARA M. A.: "Identification of critical residues in novel drug metabolizing mutants of cytochrome P450 BM3 using random mutagenesis", JOURNAL OF MEDICINAL CHEMISTRY, vol. 50, 2007, pages 455 - 461, XP002649499, DOI: doi:10.1021/jm0609061
Attorney, Agent or Firm:
PLUS INTERNATIONAL IP LAW FIRM (KR)
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