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Title:
COMPOSITION PRODUCTION METHOD, OLIGOSACCHARIDE-CONTAINING COMPOSITION OBTAINED THEREBY, AND UTILIZATION OF SAID METHOD AND COMPOSITION
Document Type and Number:
WIPO Patent Application WO/2024/043297
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for effectively utilizing a by-product generated when a dairy product is produced. Further, the present invention addresses the problem of providing a method for producing galactosyl kojibiose which has an excellent prebiotic capability. The present invention also addresses the problem of providing a composition and a solution which contain galactosyl kojibiose, the composition and the solution retaining, even after subjected to heating, at least 80% of the galactosyl kojibiose. The present invention provides a method for producing an oligosaccharide-containing composition, the method comprising a step of causing glucansucrase acting on a composition comprising sucrose and lactose to obtain a composition containing galactosyl kojibiose. It is preferable to use a lactic acid bacterium that steadily expresses membrane-bound glucansucrase. The present invention provides: a solution containing galactosyl kojibiose and having a pH of less than 7; and a composition containing galactosyl kojibiose, the composition realizing a pH of less than 7 when rendered to a solution having a solid content concentration of 10-80%.

Inventors:
ISHIDA JUNYA (JP)
ICHIKAWA AYUMI (JP)
Application Number:
PCT/JP2023/030454
Publication Date:
February 29, 2024
Filing Date:
August 24, 2023
Export Citation:
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Assignee:
MEIJI CO LTD (JP)
International Classes:
C12P19/00; C12N9/10; C12P19/44
Domestic Patent References:
WO2022039249A12022-02-24
Other References:
GARCÍA-CAYUELA TOMÁS, DÍEZ-MUNICIO MARINA, HERRERO MIGUEL, MARTÍNEZ-CUESTA M. CARMEN, PELÁEZ CARMEN, REQUENA TERESA, MORENO F. JAV: "Selective fermentation of potential prebiotic lactose-derived oligosaccharides by probiotic bacteria", INTERNATIONAL DAIRY JOURNAL, ELSEVIER APPLIED SCIENCE, BARKING,, GB, vol. 38, no. 1, 1 September 2014 (2014-09-01), GB , pages 11 - 15, XP093142685, ISSN: 0958-6946, DOI: 10.1016/j.idairyj.2014.03.012
DATABASE UniProtKB 20 January 2016 (2016-01-20), ANONYMOUS : "Full=dextransucrase {ECO:0000256|ARBA:ARBA00012592}; DE EC=2.4.1.5 {ECO:0000256|ARBA:ARBA00012592};", XP093142687, retrieved from UniProt Database accession no. A0A0R1V031
PHAM HIEN T.T., DIJKHUIZEN LUBBERT, VAN LEEUWEN SANDER S.: "Structural characterization of glucosylated lactose derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes", CARBOHYDRATE RESEARCH, PERGAMON, GB, vol. 449, 1 September 2017 (2017-09-01), GB , pages 59 - 64, XP093142686, ISSN: 0008-6215, DOI: 10.1016/j.carres.2017.07.002
Attorney, Agent or Firm:
SIKS & CO. (JP)
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