Title:
COMPOSITION FOR REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
Document Type and Number:
WIPO Patent Application WO/2011/027722
Kind Code:
A1
Abstract:
A composition for a reverse transcription polymerase chain reaction, which comprises a thermostable DNA polymerase, a reverse transcriptase, a dye marker and a specific gravity-increasing agent; and a premix reagent for a one-step RT-PCR, which comprises the composition, is not frozen under usual storage conditions at -20 to -30˚C and has excellent handleability.
Inventors:
NAKABAYASHI YUKO (JP)
SATO YOSHIMI (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
SATO YOSHIMI (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
Application Number:
PCT/JP2010/064614
Publication Date:
March 10, 2011
Filing Date:
August 27, 2010
Export Citation:
Assignee:
TAKARA BIO INC (JP)
NAKABAYASHI YUKO (JP)
SATO YOSHIMI (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
NAKABAYASHI YUKO (JP)
SATO YOSHIMI (JP)
UEMORI TAKASHI (JP)
MUKAI HIROYUKI (JP)
International Classes:
C12N15/09; C12Q1/68; G01N27/447
Foreign References:
| JPH1175897A | 1999-03-23 | |||
| JP2006503564A | 2006-02-02 |
Other References:
MALLET F. ET AL: "Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures", BIOTECHNIQUES, vol. 18, no. 4, April 1995 (1995-04-01), pages 678 - 687
WU L.Q. ET AL: "Expression of cancer-testis antigen (CTA) in tumor tissues and peripheral blood of Chinese patients with hepatocellular carcinoma", LIFE SCIENCES, vol. 79, no. 8, 2006, pages 744 - 748
WU L.Q. ET AL: "Expression of cancer-testis antigen (CTA) in tumor tissues and peripheral blood of Chinese patients with hepatocellular carcinoma", LIFE SCIENCES, vol. 79, no. 8, 2006, pages 744 - 748
Attorney, Agent or Firm:
TANAKA, Mitsuo et al. (JP)
Mitsuo Tanaka (JP)
Mitsuo Tanaka (JP)
Download PDF:
Previous Patent: DETERGENT COMPOSITION
Next Patent: SEMICONDUCTOR DEVICE AND METHOD FOR MANUFACTURING THE SAME
Next Patent: SEMICONDUCTOR DEVICE AND METHOD FOR MANUFACTURING THE SAME