This invention relates to agriculture, more specifically, to processing of grain, pulse and cereal crop grains, in particular, to methods of their enzymatic treatment before processing to food products (grits, semolina, flakes, flour etc.) with the aim of increasing the output and quality of the processed and milled product.

Grain, pulse and cereal crops have grains quite differing in shape, size and structure. Basically a grain consists of two parts: the kernel (the endosperm with the germ) and the hull (glumes). The glumes covering the kernel can be either floral (as in millets, rice, oats or barley) or fruit (as in buckwheat, wheat or popcorn) or in the form of testa (as in peas).

A very important property of a grain is the strength of the bond between the glumes and the kernel. In the grains of cereals such as rice, millets, oats and buckwheat the glumes envelop the kernel without bonding to it. In wheat, peas, popcorn and barley the glumes are tightly bonded with the kernel over its whole surface.

The highest hull content is in oats (22-30%) and the lowest one is in barley and peas. It is these grain parameters that affect the output and quality of grits and flour: bran content, size, size uniformity, humidity and impurity content. Hull content is T/RU2015/000371

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determined in grains cleaned from any impurities. The higher the hull content the lower the grits and flour output of the factory.

Grain, pulse and cereal crops have found wide use, and there are a variety of their processing methods for producing high quality food and semi-finished food.

Known is (EP No. 231729, published 12.08.1987) a method of enzymatic decomposition of whole flour to starch-containing pieces of cereals. Flour is treated with a-amylase at 80-95°C or at 100- 1 10°C and with β-amylase at 55-60°C. The enzymes are denatured by adding chloric acid and heating to 90-95 °C.

Also known is (EP No. 731646, published 1 8.09.1996) a method of producing homogeneous and stable grain suspension comprising the treatment of oatmeal with β- and a-amylases.

Further known is (US Patent 4996063, published 26.02.1991) a method of producing water-soluble dietary fiber composition by treating oatmeal or oat bran with an α-amylase for decomposing oat starch.

Known is (JP Patent 3236787, published 10.12.2001) method of producing a composition of saccharides comprising treating potato starch with an α-amylase of the genus Bacillus or splitting the branched structure of the enzyme pullulanase at 50-70°within 3-50 hours. The enzyme is then inactivated, and potato starch is further treated with β-amylase at 50-70° for 3-50 hours.

Further known is (US Patent 5458893, published 17.10.1995) a method of producing a β-glucanase treated water-soluble dietary fiber composition comprising α-amylase treatment of an aqueous dispersion of a β-glucan containing grain-based substrate before β- glucanase treatment of the water-soluble fraction.

The above methods including the enzymes and enzyme combinations used therein yield a variety of starch-containing products modified with one or more enzymes which denature starch and/or glucan by any known mechanism and therefore yield a variety of products having different properties. The enzymes are combined following different treatment methods and used at different temperatures and for different durations.

However, the above methods implemented with the above enzymes do not increase the efficiency of hydrothermal grain treatment before processing and milling and thus do not allow the factory to increase the output, quality and dietary value of its products while retaining minimum operational costs.

The closest counterpart of the present technical solution can be considered (RU Patent 2538385, published 10.01.2015) a combination of enzyme preparations: cellulases (endoglucanase (l ,4- -glucan-4-glucanohydrolase, EC/IUB # 3.2.1.4) and exoglucanases: cellobiohydrolase (1 ,4-β-Ε>- glucancellobiohydrolase, EC/IUB # 3.2.1.91 ) and/or exo-l ,4-P- glucosidase (1 ,4- β -D-glucanglucohydrolase, EC/IUB # 3.2.1 .74)) and xylanases (hemicellulases (endo-l ,4^-xylanase, EC/IUB # 3.2.1.8, and/or a-L-arabinofuranosidase, EC/IUB # 3.2.1.55)) in an amount of 10 ¹ - 10 ⁴ enzymatic activity units, as well as dietary supplements: calcium peroxide (E930) and sodium (E281 ) and/or 00371

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calcium (E282) and/or potassium (E283) propionates and/or sedum (E262), and/or calcium (E263) and or potassium (E261) acetates.

However, the experience of using said method has shown that the above enzyme combination is not efficient for hydrothermal grain treatment before milling.

Therefore the object of the present technical solution is to increase the output of flour or grit mills with a concurrent gain in the quality and dietary value of the milled products.

It is suggested to achieve said object by using the herein- provided composition for the treatment of grain, pulse and cereal crop grains before processing.

Said composition comprises a combination of enzyme preparations comprising at least cellulases and/or cellobiohydrolases and/or xylanases and/or catalases and/or phytases and/or peroxidases and/or oxidoreductases and/or laccases and/or esterases and/or hydrolases.

Said composition may further comprise antioxidants.

Said antioxidants can be ascorbates and/or tocopherols and/or polyphenols and/or natural vegetable extracts and/or citric acid and/or ascorbic acid.

Depending on the type of the raw material and the target food product said composition may comprise said enzymes in various amounts taking into account their activity.

The enzymes suggested for use herein are preferably used in combinations due to the synergism of their action. However, some embodiments may use enzyme combinations not comprising the entire list of the above enzymes but still retaining the synergism. 00371

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The synergetic action mechanism of cellulases is that enzymes of this group break the polymer molecules of insoluble pentosans contained in the grain hull to soluble high-molecular fragmentary oligosaccharides. Cellobiohydrolase opens up the cellulose fiber by hydrolyzing the cellulose molecules at the non-reducing end of the polysaccharide to form cellobiose, and then endoglucanase hydrolyzes the bonds in the cellulose molecule in a random manner producing a set of poly- and oligomeric fragments of different lengths. The partial destruction of this fiber allows β-xylanases to hydrolyze arabinoxylanes resulting in the release of the covalently bonded proteins.

Combinations of such enzymes as peroxidases and/or oxidoreductases and/or laccases and/or esterases favor the oxidation and decolorization of grain hull pigments thus providing for a greater whiteness of the milled products and a lighter color of the crump in the baked products made from the flour produced using this method. Such enzymes as catalase and/or phytase acting synergistically with the abovementioned enzymes increase the proper antioxidant activity of the crushed and milled products thus preventing the oxidative deterioration and rancidity of semolina and flour made from grain, pulse and cereal crop grains during storage.

As a result of the partial biochemical destruction of the grain hulls the grain body becomes more permeable for humidity during conditioning, the bonds between the separated hulls and the endosperm are weakened, the endosperm structure loosens faster and the grain acquires elastic-plastic or plastic properties which is generally favorable for grain processing and increases the efficiency of hullers and finishers. The milling of grains treated as above yields naturally whiter flour, i.e. increases the top grade flour output and the overall graded flour output, and improves its dietary value, the gluten content increases due to the entering of starch-containing endosperm peripheral parts into the milled product and the gluten quality is improved. Furthermore, enzymatic hydrolysis of grain hulls reduces the content of heavy metals in the milled products due to the desorption of metal ions linked with non-starch-containing polysaccharides during the solubilization of the hull structure.

The treatment of grain, pulse and cereal crop grains with the present composition before processing to semolina or flour can be combined with conditioning or hydrothermal treatment thus being incorporated into the existing process routes of factories that process any grains, grit or beans equipped with standard vessels and batchers used in the flour milling industry without additional capital costs or water consumption.

The limit consumption rates of the present composition depend on the type of raw material, its quality and the activity of the constituent enzymes.

Exceeding the top consumption limits leads to excessive hydrolysis of the grain hulls which may cause grain aggregation. Reducing the consumption to below the lower limits does affects said technical result but slightly but increases the time required for hydrothermal treatment which hinders the target increase in the quality of the final product. The present composition can be use at mills equipped with expansion vessels which can be used for the preliminary dissolution of the present composition at the conditioning stage.

The present composition is dissolved in the preliminary expansion vessel equipped with a stirrer. After cleaning the product is delivered to a screw feeder for conveying to hydrothermal treatment or conditioning together with the prepared composition solution at 10-50°C. The screw feeds the uniformly treated raw material with the composition solution to a hopper for tempering.

Furthermore, the present composition can be used at factories not equipped with expansion vessels which can be used for the preliminary dissolution of the present composition.

After cleaning the treated raw material is delivered to a screw feeder for conveying to hydrothermal treatment or conditioning together with water at 10-50°C and the present composition in a dry state which is uniformly introduced by a batcher. The screw feeds the treated raw material, water and the present composition to a hopper for tempering, uniformly stirring the materials.

The present composition can be used at any stage of hydrothermal treatment or conditioning if the existing process route of the factory contemplates this treatment in multiple stages, in dry or solute forms, as described above.

In every embodiment of the present composition the cleaned and pretreated product is then fed to the milling stage.

Examples of grain, pulse and cereal crop grain treatment composition embodiments for use before processing are presented in Table 1 : 15 000371

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Table 1

Application examples for Compositions 1 , 2 and 4: wheat milling experiments were conducted using a Nagema laboratory mill. The laboratory wheat samples were moistened to 16.5%. The enzyme combinations were dissolved in water and introduced at the hydrothermal treatment stage. The time of moistening was 12 and 18 hours. Milling of three wheat samples treated as described above provided the following final product yields: 73.5%, 79% and 82% depending on enzyme content. The total yield of the reference wheat sample (without enzyme treatment) milled in Nagema was 60.3%.

Application examples for Compositions 1 , 2 and 4: industrial milling in a 50 tpd mill. The grain tempering time was 10 hours for 00371

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each of the batches. Visual inspection of each batch suggested that the colors of the grains moistened with the enzymes were by one grade lighter than those of the grains without the enzymes. Moreover, the hulls of the experimental batch grains were more loose and shriveled. After 30 minutes of mill work the whiteness and quality of the flour were examined in each of the streams. Based on the examination results it was considered appropriate to change the grade of 2 more flour streams to the top one. As a result, of the 1 1 flour streams qualified as top grade flour, 8 were permanent throughout milling, compared to 6 for conventional grain without the enzymes. The bran of the test grain batch was cleaner, lighter and coarser compared to that of the reference sample. The overall flour yield of the reference sample was 75.5% and that of the experimental enzyme treated wheat batch was 77.24%. The presence of antioxidant in the composition for popcorn grain treatment prevents the oxidative deterioration of the milled product.

Application examples for Compositions 5 and 7: industrial milling at a 500 tpd factory. The interest to experimenting with milling at that factory was that due to the deficiency of good grain the milling batch mainly comprised medium and low grade red- grained wheat of Kazakhstan origin with a large fraction of small and shrunk grain with low grain unit. The main problem with milling this crop was the low yield (45-50%) of top grade flour.

The introduction of the enzyme combination at the conditioning stage during 6-8 h increased the top grade flour yield to 62% while retaining all the flour quality parameters. Application examples for Compositions 6 and 9: industrial milling in a 150 tpd mill. As a result the huller efficiency increased thus reducing the overall weight percentage of hulls fed to milling. Inspection revealed a drastic (natural) increase in flour whiteness for each mill and each stream (by 3 to 18 units) for wheat treated with the enzymes at the moistening stage followed by grain tempering which increased the percentage of top grade flour without compromise in the quality (including whiteness) of 1 ^st grade flour. An examination of the antioxidant activity of the bread baked from the flour processed as described above proved that this figure in the test sample was 5.6 times the one for bread baked from standard flour.

Application examples for Composition 3 : experimental milling and rolling of whole oat grain yielded the following results: cleaned oat grit was steamed and moistened to 2.0-2.5% followed by tempering. Then the grit was rolled in smooth roll machines. As a result of grain treatment with the enzyme composition the grit acquired elastic-plastic or plastic properties which generally improved its rolling, the percentage of losses (broken grain or crumbs) reduced and the wholeness of the flakes was retained. The presence of ascorbates used as antioxidants in the grit treatment composition increases its antioxidant activity.

Application examples for Composition 8: the use of the enzymes at the popcorn grain conditioning or hydrothermal treatment stages is favorable not only for the subsequent crushing and polishing operations but also for germ separation and preservation. The germ is bound to the endosperm by the cementing 2015/000371

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layer having no cell structure and consisting of protein and pentosans. As a result of moistening and the action of the enzyme composition said cementing layer softens and its bond to the endosperm weakens. This treatment may be important if the germs are used for the production of oil. The germs should be separated from the grains as completely as possible, and the endosperm content in the separated product should be as low as possible. After the treatment the germs separate in the form of coarser pieces and contain less endosperm. This technology is of special importance for the production of grit for flakes and sticks because it facilitates hull separation during crushing, and the endosperm crushes into coarser pieces which are required for flakes. The presence of tocopherol as an antioxidant in the oat grain treatment composition prevents the oxidative deterioration of the final products.

The use of the present composition increases the output of flour or grit mills with a concurrent gain in the quality and dietary value of the milled products.