Title:
COMPOSITIONS COMPRISING CELLOBIOSE DEHYDROGENASE FROM PYCNOPORUS CINNABARINUS AND THEIR USE FOR THE DEGRADATION OF LIGNOCELLULOSIC BIOMASS
Document Type and Number:
WIPO Patent Application WO/2013/004377
Kind Code:
A3
Abstract:
The invention relates to enzyme compositions comprising at least a) a cocktail of cellulases from Trichoderma reesei, and b) an enzymatic cocktail from Pycnoporus cinnabarinus comprising a cellobiose dehydrogenase (CDH) or c) a recombinant cellobiose dehydrogenase (CDH) from Pycnoporus cinnabarinus, preferably also comprising a Family 61 glycoside hydrolase. The invention also relates to methods for degrading a lignocellulosic biomass, for producing a fermentation product, for producing gluconic acid, xylonic acid and/or xylobionic acid, for enhancing the production of gluconic acid, xylonic acid and/or xylobionic acid or for increasing the yield of sugars from a lignocellulosic biomass.
Inventors:
SIGOILLOT JEAN-CLAUDE (FR)
BERRIN JEAN-GUY (FR)
BEY MATHIEU (FR)
BERRIN JEAN-GUY (FR)
BEY MATHIEU (FR)
Application Number:
PCT/EP2012/002808
Publication Date:
April 04, 2013
Filing Date:
July 04, 2012
Export Citation:
Assignee:
AGRONOMIQUE INST NAT RECH (FR)
INST FRANCAIS DU PETROLE EN NOUVELLE (FR)
AGRO IND RECH S ET DEV (FR)
SIGOILLOT JEAN-CLAUDE (FR)
BERRIN JEAN-GUY (FR)
BEY MATHIEU (FR)
INST FRANCAIS DU PETROLE EN NOUVELLE (FR)
AGRO IND RECH S ET DEV (FR)
SIGOILLOT JEAN-CLAUDE (FR)
BERRIN JEAN-GUY (FR)
BEY MATHIEU (FR)
International Classes:
C12P7/10; C12N9/04; C12N9/42; C12P7/58; C12P19/02; C12P19/12; C12P19/14; C13K1/02; D21C5/00; C07C59/105; C07H7/033
Domestic Patent References:
| WO2010080532A1 | 2010-07-15 | |||
| WO2009033071A2 | 2009-03-12 |
Other References:
MATHIEU BEY ET AL: "Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 10, no. 1, 113, 28 December 2011 (2011-12-28), pages 1 - 15, XP021093669, ISSN: 1475-2859, DOI: 10.1186/1475-2859-10-113
WENJUN BAO ET AL: "Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases", FEBS LETTERS, vol. 302, no. 1, 1 May 1992 (1992-05-01), pages 77 - 80, XP055042144, ISSN: 0014-5793, DOI: 10.1016/0014-5793(92)80289-S
MOUKHA S M ET AL: "Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase", GENE, ELSEVIER, AMSTERDAM, NL, vol. 234, no. 1, 24 June 1999 (1999-06-24), pages 23 - 33, XP004176885, ISSN: 0378-1119, DOI: 10.1016/S0378-1119(99)00189-4
SIGOILLOT C ET AL: "Lignocellulolytic and hemicellulolytic system of Pycnoporus cinnabarinus: Isolation and characterization of a cellobiose dehydrogenase and a new xylanase", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 31, no. 6, 1 November 2002 (2002-11-01), pages 876 - 883, XP002295721, ISSN: 0141-0229, DOI: 10.1016/S0141-0229(02)00208-9
DATABASE UniProt [online] 3 May 2011 (2011-05-03), "SubName: Full=Cellobiose dehydrogenase; EC=1.1.99.18; Flags: Fragment;", XP002685961, retrieved from EBI accession no. UNIPROT:F1DFN2 Database accession no. F1DFN2
ZAMOCKY M ET AL: "Cellobiose dehydrogenase - A flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi", CURRENT PROTEIN AND PEPTIDE SCIENCE, BENTHAM SCIENCE PULBISHERS, NL, vol. 7, no. 3, 1 January 2006 (2006-01-01), pages 255 - 280, XP009115339, ISSN: 1389-2037, DOI: 10.2174/138920306777452367
"Reference ID no. 80764", BRENDA ONLINE DATABASE, TU BRAUNSCHWEIG, 2010, pages 1 - 1, XP002685962, Retrieved from the Internet [retrieved on 20121025]
IGARASHI KIYOHIKO ET AL: "Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition", EUROPEAN JOURNAL OF BIOCHEMISTRY, BLACKWELL PUBLISHING, BERLIN, DE, vol. 253, no. 1, 1 April 1998 (1998-04-01), pages 101 - 106, XP002572510, ISSN: 0014-2956, DOI: 10.1046/J.1432-1327.1998.2530101.X
WENJUN BAO ET AL: "Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases", FEBS LETTERS, vol. 302, no. 1, 1 May 1992 (1992-05-01), pages 77 - 80, XP055042144, ISSN: 0014-5793, DOI: 10.1016/0014-5793(92)80289-S
MOUKHA S M ET AL: "Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase", GENE, ELSEVIER, AMSTERDAM, NL, vol. 234, no. 1, 24 June 1999 (1999-06-24), pages 23 - 33, XP004176885, ISSN: 0378-1119, DOI: 10.1016/S0378-1119(99)00189-4
SIGOILLOT C ET AL: "Lignocellulolytic and hemicellulolytic system of Pycnoporus cinnabarinus: Isolation and characterization of a cellobiose dehydrogenase and a new xylanase", ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 31, no. 6, 1 November 2002 (2002-11-01), pages 876 - 883, XP002295721, ISSN: 0141-0229, DOI: 10.1016/S0141-0229(02)00208-9
DATABASE UniProt [online] 3 May 2011 (2011-05-03), "SubName: Full=Cellobiose dehydrogenase; EC=1.1.99.18; Flags: Fragment;", XP002685961, retrieved from EBI accession no. UNIPROT:F1DFN2 Database accession no. F1DFN2
ZAMOCKY M ET AL: "Cellobiose dehydrogenase - A flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi", CURRENT PROTEIN AND PEPTIDE SCIENCE, BENTHAM SCIENCE PULBISHERS, NL, vol. 7, no. 3, 1 January 2006 (2006-01-01), pages 255 - 280, XP009115339, ISSN: 1389-2037, DOI: 10.2174/138920306777452367
"Reference ID no. 80764", BRENDA ONLINE DATABASE, TU BRAUNSCHWEIG, 2010, pages 1 - 1, XP002685962, Retrieved from the Internet
IGARASHI KIYOHIKO ET AL: "Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition", EUROPEAN JOURNAL OF BIOCHEMISTRY, BLACKWELL PUBLISHING, BERLIN, DE, vol. 253, no. 1, 1 April 1998 (1998-04-01), pages 101 - 106, XP002572510, ISSN: 0014-2956, DOI: 10.1046/J.1432-1327.1998.2530101.X
Attorney, Agent or Firm:
BARDOT, Willy (Parc Cézanne II Bat., 290 Avenue Galilée Aix-en-Provence Cedex 3, FR)
Download PDF: