Title:
COMPOSITIONS COMPRISING A MITOFUSIN INHIBITOR FOR PROMOTING CELL REPROGRAMMING AND A USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/080608
Kind Code:
A1
Abstract:
The present invention relates to a composition comprising a repressor of mitofusin gene expression, an inhibitor of mitofusin protein activity, or a mixture thereof as an active ingredient for promoting reprogramming a differentiated cell into a pluripotent stem cell, and a use thereof. The composition according to the present invention increases the efficiency of reprogramming as well as reduces the time required for reprogramming to produce pluripotent stem cells. Therefore, the present composition can be beneficially used to develop the production technology of high efficiency pluripotent stem cell and secure a large-scale culture system. Further, the present composition can be beneficially used to maintain pluripotent stem cells and screen the compounds capable of promoting the reprogramming into pluripotent stem cells.
Inventors:
CHO YEE SOOK (KR)
SON MYUNG JIN (KR)
KWON YOU JEONG (KR)
SON MYUNG JIN (KR)
KWON YOU JEONG (KR)
Application Number:
PCT/KR2015/004067
Publication Date:
May 26, 2016
Filing Date:
April 23, 2015
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
International Classes:
C12N5/02; C07K16/40; C12N5/074
Other References:
VAZQUEZ-MARTIN, A. ET AL.: "Mitochondrial fusion by pharmacological manipul ation impedes somatic cell reprogramming to pluripotency: new insight into t he role of mitophagy in cell stemness", AGING, vol. 4, no. 6, June 2012 (2012-06-01), pages 393 - 401, XP055286341
TAKAHASHI, K. ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP008155962, DOI: doi:10.1016/j.cell.2007.11.019
BUKOWIECKI, R. ET AL.: "Mitochondrial function in pluripotent stem cells an d cellular reprogramming", GERONTOLOGY, vol. 60, no. 2, 2014, pages 174 - 182, XP055285446
CHERRY, ABC. ET AL.: "Induced pluripotent stem cells with a mitochondrial D NA deletion", STEM CELLS, vol. 31, no. 7, July 2013 (2013-07-01), pages 1287 - 1297, XP055285444, DOI: doi:10.1002/stem.1354
SON, MJ ET AL.: "Mitofusins deficiency elicits mitochondrial metabolic rep rogramming to pluripotency", CELL DEATH & DIFFERENTIATION, 17 April 2015 (2015-04-17), pages 1 - 10, XP055285430
TAKAHASHI K ET AL., CELL, vol. 126, no. 4, 2006, pages 663 - 676
BUGANIM Y ET AL., CELL, vol. 150, no. 6, 2012, pages 1209 - 1222
TAKAHASHI, K. ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP008155962, DOI: doi:10.1016/j.cell.2007.11.019
BUKOWIECKI, R. ET AL.: "Mitochondrial function in pluripotent stem cells an d cellular reprogramming", GERONTOLOGY, vol. 60, no. 2, 2014, pages 174 - 182, XP055285446
CHERRY, ABC. ET AL.: "Induced pluripotent stem cells with a mitochondrial D NA deletion", STEM CELLS, vol. 31, no. 7, July 2013 (2013-07-01), pages 1287 - 1297, XP055285444, DOI: doi:10.1002/stem.1354
SON, MJ ET AL.: "Mitofusins deficiency elicits mitochondrial metabolic rep rogramming to pluripotency", CELL DEATH & DIFFERENTIATION, 17 April 2015 (2015-04-17), pages 1 - 10, XP055285430
TAKAHASHI K ET AL., CELL, vol. 126, no. 4, 2006, pages 663 - 676
BUGANIM Y ET AL., CELL, vol. 150, no. 6, 2012, pages 1209 - 1222
Attorney, Agent or Firm:
SON, Min (6th Floor 163, YangJaeCheon-ro,,Gangnam-gu, Seoul 135-855, KR)
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