Title:
COMPOSITIONS CONTAINING BIOEMULSIFIERS AND A METHOD FOR THEIR PREPARATION
Document Type and Number:
WIPO Patent Application WO2002048327
Kind Code:
A3
Abstract:
A bioemulsifier composition useful for forming and stabilizing oil-in-water emulsions, comprising an esterase protein of 32.5 KD, found in association with emulsan in the bacteria Acinetobacter. The esterase, or parts of it, are isolated from cell extracts of various strains of Acinetobacter, or produced by any other means. The bioemulsifier composition is further comprised of a water-soluble polysaccharide polymer of any source. The present invention further discloses a method of forming and stabilizing oil-in-water emulsions, using the above-mentioned composition.
Inventors:
GUTNICK DAVID L (IL)
BACH HORACIO R (IL)
BACH HORACIO R (IL)
Application Number:
PCT/IL2001/001155
Publication Date:
January 03, 2003
Filing Date:
December 12, 2001
Export Citation:
Assignee:
UNIV RAMOT (IL)
GUTNICK DAVID L (IL)
BACH HORACIO R (IL)
GUTNICK DAVID L (IL)
BACH HORACIO R (IL)
International Classes:
C09K23/00; C12N9/16; (IPC1-7): B01F17/00; C02F1/52; C12N9/18
Foreign References:
| EP0016546A1 | 1980-10-01 | |||
| US5840547A | 1998-11-24 |
Other References:
REDDY P G ET AL: "CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF AN ESTERASE-CODING GENE FROM THE OIL-DEGRADING BACTERIUM ACINETOBACTER-CALCOACETICUS RAG-1", GENE (AMSTERDAM), vol. 76, no. 1, P18773, 1989, pages 145 - 152, XP001106518, ISSN: 0378-1119
ALON RINAT N ET AL: "Esterase from the oil-degrading Acinetobacter lwoffi RAG-1: Sequence analysis and over-expression in Escherichia coli.", FEMS (FEDERATION OF EUROPEAN MICROBIOLOGICAL SOCIETIES) MICROBIOLOGY, vol. 112, no. 3, 1993, 1993, pages 275 - 280, XP002216598, ISSN: 0378-1097
GUTNICK D L ET AL: "Engineering bacterial biopolymers for the biosorption of heavy metals;new products and novel formulations.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 54, no. 4, October 2000 (2000-10-01), pages 451 - 460, XP002216599, ISSN: 0175-7598
CHEN WILFRED ET AL: "Engineering of improved microbes and enzymes for bioremediation.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 10, no. 2, April 1999 (1999-04-01), pages 137 - 141, XP002216600, ISSN: 0958-1669
ALON RINAT N ET AL: "Esterase from the oil-degrading Acinetobacter lwoffi RAG-1: Sequence analysis and over-expression in Escherichia coli.", FEMS (FEDERATION OF EUROPEAN MICROBIOLOGICAL SOCIETIES) MICROBIOLOGY, vol. 112, no. 3, 1993, 1993, pages 275 - 280, XP002216598, ISSN: 0378-1097
GUTNICK D L ET AL: "Engineering bacterial biopolymers for the biosorption of heavy metals;new products and novel formulations.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 54, no. 4, October 2000 (2000-10-01), pages 451 - 460, XP002216599, ISSN: 0175-7598
CHEN WILFRED ET AL: "Engineering of improved microbes and enzymes for bioremediation.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 10, no. 2, April 1999 (1999-04-01), pages 137 - 141, XP002216600, ISSN: 0958-1669
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