Title:
COMPOSITIONS CONTAINING PROTEIN LOADED EXOSOME AND METHODS FOR PREPARING AND DELIVERING THE SAME
Document Type and Number:
WIPO Patent Application WO/2018/062973
Kind Code:
A1
Abstract:
The present invention relates to a method for the mass-production of exosome comprising a cargo protein, a vector for preparing the exosome, exosome including a cargo protein prepared by the method, and a method for loading the cargo protein to cytosol by using the exosome prepared thereby. According to the method for preparing an exosome comprising a cargo protein provided by the present invention, the exosome loaded with a cargo protein can be produced with a high yield, so that it can be used broadly in the treatment of disease using the exosome.
Inventors:
CHOI CHULHEE (KR)
CHOI KYUNGSUN (KR)
RYU SEUNG-WOOK (KR)
YIM NAMBIN (KR)
CHOI HOJUN (KR)
CHOI KYUNGSUN (KR)
RYU SEUNG-WOOK (KR)
YIM NAMBIN (KR)
CHOI HOJUN (KR)
Application Number:
PCT/KR2017/011070
Publication Date:
April 05, 2018
Filing Date:
September 29, 2017
Export Citation:
Assignee:
CELLEX LIFE SCIENCES INCORPORATED (KR)
International Classes:
C12N9/24; A61K9/127; A61K38/47; C12N9/22; C12N15/113; C12N15/63; C12N15/85; C12N15/88
Domestic Patent References:
| WO2014168548A2 | 2014-10-16 | |||
| WO2009015357A1 | 2009-01-29 |
Foreign References:
| KR20120081972A | 2012-07-20 | |||
| KR20160130949A | 2016-11-15 | |||
| KR20100127768A | 2010-12-06 | |||
| KR20040015508A | 2004-02-19 |
Other References:
KENNEDY, MATTHEW J. ET AL.: "Rapid blue-light-mediated induction of protein interactions in living cells", NATURE METHODS, vol. 7, no. 12, 2010, pages 973 - 975, XP055039821
LIU, ZHIQING ET AL.: "Direct activation of Bax protein for cancer therapy", MEDICINAL RESEARCH REVIEWS, vol. 36, no. 2, March 2016 (2016-03-01), pages 313 - 341, XP055501005
"Catalog No. 631449", CLONTECH LABORATORIES, 29 September 2015 (2015-09-29), Retrieved from the Internet
NAMBIN Y. ET AL., NAT. COMMUN., vol. 7, 2016, pages 1 - 7
NAMBIN Y.: "Supplementary Information filed for: Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module", NAT. COMMUN., 22 July 2016 (2016-07-22)
STEVEN R ET AL.: "Protein transduction: unrestricted delivery into all cells", TRENDS IN CELL BIOLOGY, 2000
MUNISH CHANANA ET AL.: "Physicochemical properties of protein-coated gold nanoparticles in biological fluids and cells before and after proteolytic digestion", ANGEW. CHEM. INT. ED., 2013
GRACA RAPOSOWILLEM STOORVOGEL: "Extracellular vesicles: Exosomes, microvesicles, and friends", CELL BIOLOGY, vol. 200, no. 4, 2013, pages 373 - 383
ALVAREZ-ERVITI, L. ET AL.: "Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes", NATURE BIOTECHNOLOGY, vol. 29, 2011, pages 341 - 345, XP055554900, DOI: 10.1038/nbt.1807
LAPPAS ET AL., BIOL. REPROD., vol. 67, 2002, pages 668673
See also references of EP 3356522A4
LIU, ZHIQING ET AL.: "Direct activation of Bax protein for cancer therapy", MEDICINAL RESEARCH REVIEWS, vol. 36, no. 2, March 2016 (2016-03-01), pages 313 - 341, XP055501005
"Catalog No. 631449", CLONTECH LABORATORIES, 29 September 2015 (2015-09-29), Retrieved from the Internet
NAMBIN Y. ET AL., NAT. COMMUN., vol. 7, 2016, pages 1 - 7
NAMBIN Y.: "Supplementary Information filed for: Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module", NAT. COMMUN., 22 July 2016 (2016-07-22)
STEVEN R ET AL.: "Protein transduction: unrestricted delivery into all cells", TRENDS IN CELL BIOLOGY, 2000
MUNISH CHANANA ET AL.: "Physicochemical properties of protein-coated gold nanoparticles in biological fluids and cells before and after proteolytic digestion", ANGEW. CHEM. INT. ED., 2013
GRACA RAPOSOWILLEM STOORVOGEL: "Extracellular vesicles: Exosomes, microvesicles, and friends", CELL BIOLOGY, vol. 200, no. 4, 2013, pages 373 - 383
ALVAREZ-ERVITI, L. ET AL.: "Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes", NATURE BIOTECHNOLOGY, vol. 29, 2011, pages 341 - 345, XP055554900, DOI: 10.1038/nbt.1807
LAPPAS ET AL., BIOL. REPROD., vol. 67, 2002, pages 668673
See also references of EP 3356522A4
Attorney, Agent or Firm:
LEE, Won Hee (KR)
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