Title:
COMPOSITIONS AND METHODS FOR DETECTING A SEQUENCE MUTATION IN THE CINNAMYL ALCOHOL DEHYDROGENASE GENE ASSOCIATED WITH ALTERED LIGNIFICATION IN LOBLOLLY PINE
Document Type and Number:
WIPO Patent Application WO2004072237
Kind Code:
A3
Abstract:
Loblolly pine (Pinus taeda L.) is the most important commercial tree species in the USA harvested for pulp and solid wood products. Increasing the efficiency of chemical pulping may be achieved through the manipulation of genes involved in the lignin biosynthetic pathway. A null allele of cinnamyl alcohol dehydrogenase (CAD) has been discovered in the loblolly pine clone 7-56 which displays altered lignin composition. During identification of single nucleotide polymorphisms (SNPs) in the cad gene, a two-base pair adenosine insertion located in exon five and unique to clone 7-56 was discovered. The sequence mutation causes a frame-shift predicted to result in premature termination of the protein. For routine detection of the mutation, a diagnostic assay was developed utilizing Template-directed Dye-terminator Incorporation and Fluorescence Polarization detection (FT-TDI).
Inventors:
GILL GEOFFREY P (US)
BROWN GARTH R (US)
NEALE DAVID B (US)
BROWN GARTH R (US)
NEALE DAVID B (US)
Application Number:
PCT/US2004/003112
Publication Date:
June 09, 2005
Filing Date:
February 04, 2004
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
US AGRICULTURE (US)
GILL GEOFFREY P (US)
BROWN GARTH R (US)
NEALE DAVID B (US)
US AGRICULTURE (US)
GILL GEOFFREY P (US)
BROWN GARTH R (US)
NEALE DAVID B (US)
International Classes:
A01H7/00; C07H21/04; C12Q1/68; C12N; C12Q; (IPC1-7): C07H21/04; C12Q/
Other References:
WU ET AL: "Average effect of mutation in lignin biosynthesis in loblolly pine", THEOR APPL GENET, vol. 99, 1999, pages 705 - 710, XP035065879, DOI: doi:10.1007/s001220051287
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