Title:
COMPOSITIONS AND METHODS FOR EFFECTIVE DELIVERY OF POLYNUCLEOTIDES TO CELLS
Document Type and Number:
WIPO Patent Application WO/2023/001156
Kind Code:
A1
Abstract:
Provided are compositions and methods for effective delivery of polynucleotides into the nuclei of cells through electroporation. A new buffer system is provided that facilitates the delivery with greatly reduced cell toxicity. The buffer may include succinate, mannitol, a sugar, glutamine or an analog, and an antioxidant. Also provided are methods that are particularly suitable for delivering an RNA or protein into a cell by electrophoresis in a solution having an osmotic pressure greater than 310 mOsmol/kg.
Inventors:
ZHANG YING (CN)
YIN HAO (CN)
ZHANG CHUANPING (CN)
JING AN (CN)
YIN HAO (CN)
ZHANG CHUANPING (CN)
JING AN (CN)
Application Number:
PCT/CN2022/106565
Publication Date:
January 26, 2023
Filing Date:
July 19, 2022
Export Citation:
Assignee:
UNIV WUHAN (CN)
International Classes:
C12N15/85; C12N13/00
Domestic Patent References:
| WO2018098376A1 | 2018-05-31 | |||
| WO2001093836A2 | 2001-12-13 |
Foreign References:
| US20050064596A1 | 2005-03-24 | |||
| CN111117967A | 2020-05-08 | |||
| CN113583953A | 2021-11-02 | |||
| CN1078261A | 1993-11-10 |
Other References:
EUSÉBIO DALINDA, NEVES ANA R., COSTA DIANA, BISWAS SWATI, ALVES GILBERTO, CUI ZHENGRONG, SOUSA ÂNGELA: "Methods to improve the immunogenicity of plasmid DNA vaccines", DRUG DISCOVERY TODAY, ELSEVIER, AMSTERDAM, NL, vol. 26, no. 11, 1 November 2021 (2021-11-01), AMSTERDAM, NL , pages 2575 - 2592, XP093026640, ISSN: 1359-6446, DOI: 10.1016/j.drudis.2021.06.008
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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