Title:
COMPOSITIONS AND METHODS FOR ENHANCED AMINO ACID LEVELS IN PLANTS
Document Type and Number:
WIPO Patent Application WO/2010/002876
Kind Code:
A3
Abstract:
Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feedback-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
Inventors:
CROWLEY JAMES H
GOLDMAN BARRY S
HUANG JINTAI
QI QUNGANG
RAPP WILLIAM D
GOLDMAN BARRY S
HUANG JINTAI
QI QUNGANG
RAPP WILLIAM D
Application Number:
PCT/US2009/049233
Publication Date:
March 11, 2010
Filing Date:
June 30, 2009
Export Citation:
Assignee:
MONSANTO TECHNOLOGY LLC (US)
CROWLEY JAMES H
GOLDMAN BARRY S
HUANG JINTAI
QI QUNGANG
RAPP WILLIAM D
CROWLEY JAMES H
GOLDMAN BARRY S
HUANG JINTAI
QI QUNGANG
RAPP WILLIAM D
International Classes:
C12N15/54; C12N15/82
Domestic Patent References:
| WO1995015392A1 | 1995-06-08 | |||
| WO1993019190A1 | 1993-09-30 | |||
| WO2002094867A2 | 2002-11-28 |
Foreign References:
| EP0485970A2 | 1992-05-20 | |||
| US20030233675A1 | 2003-12-18 |
Other References:
KARCHI H ET AL: "SEED-SPECIFIC EXPRESSION OF A BACTERIAL DESENSITIZED ASPARTATE KINASE INCRASES THE PRODUCTION OF SEED THREONINE AND METHIONINE IN TRANSGENIC TOBACCO", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 3, no. 5, 1 January 1993 (1993-01-01), pages 721 - 727, XP002015695, ISSN: 0960-7412
Attorney, Agent or Firm:
HANSON, Robert, E. (7800 Sears Tower233 South Wacker Dr, Chicago IL, US)
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