Title:
COMPOSITIONS AND METHODS FOR ENHANCING PRODUCTION OF A BIOLOGICAL PRODUCT
Document Type and Number:
WIPO Patent Application WO/2011/056911
Kind Code:
A3
Abstract:
Provided herein are methods, nucleic acids, polypeptides, compositions, and kits relating to the conjugation of a heterologous polypeptide to a molecule of interest during production of the polypeptide in cell culture. In various embodiments, the heterologous polypeptide is linked to a sortase ligation sequence and the molecule of interest is linked to a complementary sortase ligation sequence, such that expression of the heterologous protein in the presence of the molecule of interest and cells expressing a surface-associated sortase with the sortase catalytic domain exposed to the extracellular medium results in ligation of the heterologous polypeptide to the molecule of interest to form a conjugated polypeptide.
Inventors:
ROSSOMANDO ANTHONY (US)
Application Number:
PCT/US2010/055355
Publication Date:
October 06, 2011
Filing Date:
November 04, 2010
Export Citation:
Assignee:
ALNYLAM PHARMACEUTICALS INC (US)
ROSSOMANDO ANTHONY (US)
ROSSOMANDO ANTHONY (US)
International Classes:
C12N15/52; C07K19/00; C12N15/62; C12N15/63; C12R1/445
Domestic Patent References:
| WO2005051976A2 | 2005-06-09 |
Other References:
PARTHASARATHY, R. ET AL.: "Sortase A as a novel molecular ''stapler'' for seque nce-specific protein conjugation", BIOCONJUGATE CHEM., vol. 18, no. 2, 16 February 2007 (2007-02-16), pages 469 - 476
MAO, H. ET AL.: "Sortase-mediated protein ligation: A new method for protein engineering", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 9, 10 February 2004 (2004-02-10), pages 2670 - 2671, XP055060278, DOI: doi:10.1021/ja039915e
PRITZ, S. ET AL.: "Synthesis of biologically active peptide nucleic acid-pept ide conjugates by sortase-mediated ligation", J. ORG. CHEM., vol. 72, no. 10, 14 April 2007 (2007-04-14), pages 3909 - 3912
MAO, H. ET AL.: "Sortase-mediated protein ligation: A new method for protein engineering", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 9, 10 February 2004 (2004-02-10), pages 2670 - 2671, XP055060278, DOI: doi:10.1021/ja039915e
PRITZ, S. ET AL.: "Synthesis of biologically active peptide nucleic acid-pept ide conjugates by sortase-mediated ligation", J. ORG. CHEM., vol. 72, no. 10, 14 April 2007 (2007-04-14), pages 3909 - 3912
Attorney, Agent or Firm:
WARD, Donna, T. (P.C.292 Main Street, Suite, Groton Massachusetts, US)
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