Title:
COMPOSITIONS AND METHODS FOR GENOME EDITING
Document Type and Number:
WIPO Patent Application WO/2018/226972
Kind Code:
A3
Abstract:
The present disclosure provides compositions, methods, and systems for targeted plant genome editing. In some embodiments, the present disclosure provides recombinant self-replicating RNAs derived from a plant virus vector, such as those derived from Tobacco Mosaic Virus (TMV). In some embodiments, the recombinant self-replicating RNAs direct the expression of both a CRISPR endonuclease and a guide RNA capable of directing sequence-specific binding of the CRISPR endonuclease to a target DNA in the genome of plant cell.
Inventors:
LINDBO JOHN (US)
Application Number:
PCT/US2018/036480
Publication Date:
February 20, 2020
Filing Date:
June 07, 2018
Export Citation:
Assignee:
VILMORIN & CIE (FR)
International Classes:
C12N15/82; C12N9/22; C12N15/113; C12N15/86; C12N15/90
Domestic Patent References:
| WO2014144155A1 | 2014-09-18 | |||
| WO2016094872A1 | 2016-06-16 |
Other References:
LINDBO, JA: "TRBO: A High-Efficiency Tobacco Mosaic Virus RNA-Based Overexpression Vector", PLANT PHYSIOLOGY, vol. 145, no. 4, 24 August 2007 (2007-08-24), pages 1232 - 1240, XP055687168, DOI: 10.1104/pp.107.106377
OLEA, C ET AL.: "Real-Time Detection of a Self-Replicating RNA Enzyme", MOLECULES, vol. 21, no. 10, 30 September 2016 (2016-09-30), pages 1 - 12, XP055687177, DOI: 10.3390/molecules21101310
OLEA, C ET AL.: "Real-Time Detection of a Self-Replicating RNA Enzyme", MOLECULES, vol. 21, no. 10, 30 September 2016 (2016-09-30), pages 1 - 12, XP055687177, DOI: 10.3390/molecules21101310
Attorney, Agent or Firm:
VEITENHEIMER, Erich et al. (US)
Download PDF: