Title:
COMPOSITIONS AND METHODS FOR INCREASING DELETION EFFICIENCY OF NUCLEIC ACID SEGMENT BY MODULATION OF NHEJ REPAIR PATHWAY
Document Type and Number:
WIPO Patent Application WO/2023/172115
Kind Code:
A1
Abstract:
The present invention relates to a composition and method for increasing deletion efficiency of nucleic acid segments in a target gene by modulation of non-homologous end joining (NHEJ) repair pathway. Specifically, the present invention relates to a CRISPR/Cas system (for example, CRISPR/Cas12f1 system) for increasing deletion efficiency of nucleic acid segments in a target gene by modulation of non-homologous end joining repair pathway.
Inventors:
KIM YONG SAM (KR)
KIM DO YON (KR)
CHIN HYUN JUNG (KR)
KIM DO YON (KR)
CHIN HYUN JUNG (KR)
Application Number:
PCT/KR2023/003336
Publication Date:
September 14, 2023
Filing Date:
March 10, 2023
Export Citation:
Assignee:
GENKORE INC (KR)
International Classes:
C12N9/12; A61K48/00; A61P21/04; C12N9/22; C12N15/10; C12N15/113; C12N15/86
Foreign References:
| KR20210053228A | 2021-05-11 | |||
| KR20190058358A | 2019-05-29 | |||
| US20190338311A1 | 2019-11-07 |
Other References:
ZHU LI, MON HIROAKI, XU JIAN, LEE JAE MAN, KUSAKABE TAKAHIRO: "CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells", SCIENTIFIC REPORTS, vol. 5, no. 1, 10 December 2015 (2015-12-10), XP093067283, DOI: 10.1038/srep18103
SUPRIYA V. VARTAK, SATHEES C. RAGHAVAN: "Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing", THE FEBS JOURNAL, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 282, no. 22, 1 November 2015 (2015-11-01), GB , pages 4289 - 4294, XP055342300, ISSN: 1742-464X, DOI: 10.1111/febs.13416
SUPRIYA V. VARTAK, SATHEES C. RAGHAVAN: "Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing", THE FEBS JOURNAL, WILEY-BLACKWELL PUBLISHING LTD., GB, vol. 282, no. 22, 1 November 2015 (2015-11-01), GB , pages 4289 - 4294, XP055342300, ISSN: 1742-464X, DOI: 10.1111/febs.13416
Attorney, Agent or Firm:
SEUM IP (KR)
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