Title:
COMPOSITIONS AND METHODS FOR NUCLEIC ACID BASED DIAGNOSTIC ASSAYS
Document Type and Number:
WIPO Patent Application WO/2012/048207
Kind Code:
A3
Abstract:
The present invention relates to compositions and methods for nucleic acid based diagnostic assays. In particular, the present invention provides probes and non-amplifiable controls for asymmetric PCR and other amplification modalities. In some embodiments, the present invention provides probe design criteria for probes for use in amplification/detection assays. Further embodiments of the present invention provide non-amplifiable controls for use in generating reference probe signal ratios in amplification detection assays.
Inventors:
WANGH LAWRENCE J (US)
SANCHEZ J AQUILES (US)
SANCHEZ J AQUILES (US)
Application Number:
PCT/US2011/055237
Publication Date:
July 19, 2012
Filing Date:
October 07, 2011
Export Citation:
Assignee:
UNIV BRANDEIS (US)
WANGH LAWRENCE J (US)
SANCHEZ J AQUILES (US)
WANGH LAWRENCE J (US)
SANCHEZ J AQUILES (US)
International Classes:
C12N15/11; C12Q1/68
Foreign References:
| US20060177841A1 | 2006-08-10 | |||
| US20040053254A1 | 2004-03-18 |
Other References:
SANCHEZ, J. A. ET AL.: "Two-temperature LATE-PCR endpoint genotyping.", BMC BIOTECHNOLOGY., vol. 6, no. 44, 4 December 2006 (2006-12-04), pages 1 - 14
SANCHEZ, J. A. ET AL.: "Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 101, no. 7, 17 February 2004 (2004-02-17), pages 1933 - 8, XP002990854, DOI: doi:10.1073/pnas.0305476101
SANCHEZ, J. A. ET AL.: "Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 101, no. 7, 17 February 2004 (2004-02-17), pages 1933 - 8, XP002990854, DOI: doi:10.1073/pnas.0305476101
Attorney, Agent or Firm:
BOND, Jason, R. (Suite 310Middleton, Wisconsin, US)
Download PDF:
Previous Patent: METHOD AND SYSTEM FOR DYNAMICALLY OBSCURING ADDRESSES IN IPV6
Next Patent: DRY CLEANING SOLVENT
Next Patent: DRY CLEANING SOLVENT