Title:
COMPOSITIONS AND METHODS FOR OVERPRODUCTION OF HOMOGENTISIC ACID AND PYOMELANIN ISOFORMS IN YEAST
Document Type and Number:
WIPO Patent Application WO/2023/049380
Kind Code:
A3
Abstract:
Provided herein are genetically modified yeast cells engineered for increased production amounts and rates of biosynthetic homogentisic acid and biosynthetic pyomelanin. Also provided are methods using the disclosed genetically modified yeast cells, biosynthetic homogentisic acid, and biosynthetic pyomelanin.
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Inventors:
ALPER HAL S (US)
MILLER KELLY K (US)
MILLER KELLY K (US)
Application Number:
PCT/US2022/044584
Publication Date:
November 09, 2023
Filing Date:
September 23, 2022
Export Citation:
Assignee:
UNIV TEXAS (US)
International Classes:
C12N15/52; A61K36/06; C12N9/00; C12N15/09
Foreign References:
| US20210180092A1 | 2021-06-17 | |||
| US20190058193A1 | 2019-02-21 | |||
| US5846531A | 1998-12-08 |
Other References:
TOKUHARA YASUNORI, SHUKUYA KENICHI, TANAKA MASAMI, MOURI MARIKO, OHKAWA RYUNOSUKE, FUJISHIRO MIDORI, TAKAHASHI TOMOO, OKUBO SHIGEO: "Detection of Novel Visible-Light Region Absorbance Peaks in the Urine after Alkalization in Patients with Alkaptonuria", PLOS ONE, vol. 9, no. 1, 23 January 2014 (2014-01-23), US , pages 1 - 7, XP093108230, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0086606
ALMEIDA-PAES RODRIGO, ALMEIDA-SILVA FERNANDO, PINTO GABRIELA COSTA MAIA, ALMEIDA MARCOS DE ABREU, MUNIZ MAURO DE MEDEIROS, PIZZINI: "L-tyrosine induces the production of a pyomelanin-like pigment by the parasitic yeast-form of Histoplasma capsulatum", MEDICAL MYCOLOGY, vol. 56, no. 4, 1 June 2018 (2018-06-01), Abingdon , pages 506 - 509, XP093108232, ISSN: 1369-3786, DOI: 10.1093/mmy/myx068
ARIAS-BARRAU ELSA, OLIVERA ELÍAS R., LUENGO JOSÉ M., FERNÁNDEZ CRISTINA, GALÁN BEATRIZ, GARCÍA JOSÉ L., DÍAZ EDUARDO, MIÑA: "The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida", JOURNAL OF BACTERIOLOGY, vol. 186, no. 15, 1 August 2004 (2004-08-01), US , pages 5062 - 5077, XP093108233, ISSN: 0021-9193, DOI: 10.1128/JB.186.15.5062-5077.2004
CARREIRA ALEXANDRA, FERREIRA LUÍSA M., LOUREIRO VIRGÍLIO: "Brown Pigments Produced by Yarrowia lipolytica Result from Extracellular Accumulation of Homogentisic Acid", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 8, 1 August 2001 (2001-08-01), US , pages 3463 - 3468, XP093108235, ISSN: 0099-2240, DOI: 10.1128/AEM.67.8.3463-3468.2001
GALEB HANAA A., LAMANTIA ANGELO, ROBSON ALEXANDER, KÖNIG KATJA, EICHHORN JONAS, BALDOCK SARA J., ASHTON MARK D., BAUM JOHN V., MOR: "The Polymerization of Homogentisic Acid In Vitro as a Model for Pyomelanin Formation", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 223, no. 6, 1 March 2022 (2022-03-01), DE , pages 1 - 10, XP093108239, ISSN: 1022-1352, DOI: 10.1002/macp.202100489
MILLER KELLY K., SPRINGTHORPE SARAH K., IMBROGNO JENNIFER, WALKER DAVID J. F., GADIYAR SHRUTI, KEITZ BENJAMIN K., ELLINGTON ANDREW: "Biocompatible Materials Enabled by Biobased Production of Pyomelanin Isoforms Using an Engineered Yarrowia lipolytica", ADVANCED FUNCTIONAL MATERIALS, vol. 32, no. 7, 1 February 2022 (2022-02-01), DE , pages 1 - 11, XP093108243, ISSN: 1616-301X, DOI: 10.1002/adfm.202109366
ALMEIDA-PAES RODRIGO, ALMEIDA-SILVA FERNANDO, PINTO GABRIELA COSTA MAIA, ALMEIDA MARCOS DE ABREU, MUNIZ MAURO DE MEDEIROS, PIZZINI: "L-tyrosine induces the production of a pyomelanin-like pigment by the parasitic yeast-form of Histoplasma capsulatum", MEDICAL MYCOLOGY, vol. 56, no. 4, 1 June 2018 (2018-06-01), Abingdon , pages 506 - 509, XP093108232, ISSN: 1369-3786, DOI: 10.1093/mmy/myx068
ARIAS-BARRAU ELSA, OLIVERA ELÍAS R., LUENGO JOSÉ M., FERNÁNDEZ CRISTINA, GALÁN BEATRIZ, GARCÍA JOSÉ L., DÍAZ EDUARDO, MIÑA: "The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida", JOURNAL OF BACTERIOLOGY, vol. 186, no. 15, 1 August 2004 (2004-08-01), US , pages 5062 - 5077, XP093108233, ISSN: 0021-9193, DOI: 10.1128/JB.186.15.5062-5077.2004
CARREIRA ALEXANDRA, FERREIRA LUÍSA M., LOUREIRO VIRGÍLIO: "Brown Pigments Produced by Yarrowia lipolytica Result from Extracellular Accumulation of Homogentisic Acid", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 8, 1 August 2001 (2001-08-01), US , pages 3463 - 3468, XP093108235, ISSN: 0099-2240, DOI: 10.1128/AEM.67.8.3463-3468.2001
GALEB HANAA A., LAMANTIA ANGELO, ROBSON ALEXANDER, KÖNIG KATJA, EICHHORN JONAS, BALDOCK SARA J., ASHTON MARK D., BAUM JOHN V., MOR: "The Polymerization of Homogentisic Acid In Vitro as a Model for Pyomelanin Formation", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 223, no. 6, 1 March 2022 (2022-03-01), DE , pages 1 - 10, XP093108239, ISSN: 1022-1352, DOI: 10.1002/macp.202100489
MILLER KELLY K., SPRINGTHORPE SARAH K., IMBROGNO JENNIFER, WALKER DAVID J. F., GADIYAR SHRUTI, KEITZ BENJAMIN K., ELLINGTON ANDREW: "Biocompatible Materials Enabled by Biobased Production of Pyomelanin Isoforms Using an Engineered Yarrowia lipolytica", ADVANCED FUNCTIONAL MATERIALS, vol. 32, no. 7, 1 February 2022 (2022-02-01), DE , pages 1 - 11, XP093108243, ISSN: 1616-301X, DOI: 10.1002/adfm.202109366
Attorney, Agent or Firm:
ROBERGE, Christopher M. et al. (US)
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