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Patent Searching and Data


Title:
COMPOSITIONS AND METHODS FOR RAPID AND DYNAMIC FLUX CONTROL USING SYNTHETIC METABOLIC VALVES
Document Type and Number:
WIPO Patent Application WO/2016/053397
Kind Code:
A3
Abstract:
This invention relates to metabolically engineered microorganisms, such as bacterial and or fungal strains, and bioprocesses utilizing such strains. These strains enable the dynamic control of metabolic pathways, which can be used to optimize production. Dynamic control over metabolism is accomplished via a combination of methodologies including but not limited to transcriptional silencing and controlled enzyme proteolysis. These microbial strains are utilized in a multi-stage bioprocess encompassing at least two stages, the first stage in which microorganisms are grown and metabolism can be optimized for microbial growth and at least one other stage in which growth can be slowed or stopped, and dynamic changes can be made to metabolism to improve the production of desired product, such as a chemical or fuel.

Inventors:
LYNCH MICHAEL DAVID (US)
TRAHAN ASHLEY DELANIE (US)
RODRIGUEZ DANIEL (US)
YE ZHIXIA (US)
COOPER CHARLIE (US)
BOZDAG AHMET (US)
Application Number:
PCT/US2015/035306
Publication Date:
June 16, 2016
Filing Date:
June 11, 2015
Export Citation:
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Assignee:
UNIV DUKE (US)
LYNCH MICHAEL DAVID (US)
TRAHAN ASHLEY DELANIE (US)
RODRIGUEZ DANIEL (US)
YE ZHIXIA (US)
COOPER CHARLIE (US)
BOZDAG AHMET (US)
International Classes:
C12N15/63; C12N1/15; C12N1/21; C12N9/22; C12N15/113
Domestic Patent References:
WO2008141174A22008-11-20
Foreign References:
US20120052547A12012-03-01
EP2562249A12013-02-27
US20120107892A12012-05-03
Other References:
FANG, SHI-MING ET AL.: "A practical strategy to discover new antitumor comp ounds by activating silent metabolite production in fungi by diethyl sulphat e mutagenesis", MARINE DRUGS, vol. 12, no. 4, 27 March 2014 (2014-03-27), pages 1788 - 1814, XP055327793
Attorney, Agent or Firm:
ERGENZINGER, Edward R. et al. (LLC125 Edinburgh South Drive,Suite 22, Cary NC, US)
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