Title:
COMPOSITIONS AND METHODS FOR TERMINATING A SEQUENCING REACTION AT A SPECIFIC LOCATION IN A TARGET DNA TEMPLATE
Document Type and Number:
WIPO Patent Application WO2006081426
Kind Code:
A3
Abstract:
Compositions and methods for sequencing a template polynucleotide comprising a sequence of interest are provided herein. The compositions and methods employ at least one blocking probe that is designed to bind in a sequence-specific manner to a blocking sequence such that primer extension beyond the site where the blocking probe binds is reduced or prevented.
Inventors:
CHEN SHIAW-MIN (US)
BRANDIS JOHN (US)
BRANDIS JOHN (US)
Application Number:
PCT/US2006/002941
Publication Date:
October 19, 2006
Filing Date:
January 27, 2006
Export Citation:
Assignee:
APPLERA CORP (US)
CHEN SHIAW-MIN (US)
BRANDIS JOHN (US)
CHEN SHIAW-MIN (US)
BRANDIS JOHN (US)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2003035671A2 | 2003-05-01 | |||
| WO1994017207A1 | 1994-08-04 | |||
| WO2005093101A1 | 2005-10-06 |
Other References:
GIDDINGS M C ET AL: "A software system for data analysis in automated DNA sequencing", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 8, no. 6, June 1998 (1998-06-01), pages 644 - 665, XP002255825, ISSN: 1088-9051
SANGER F ET AL: "DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, NEW YORK, NY, US, vol. 74, no. 12, 1 December 1977 (1977-12-01), pages 5463 - 5467, XP000603873
SANGER F ET AL: "DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, NEW YORK, NY, US, vol. 74, no. 12, 1 December 1977 (1977-12-01), pages 5463 - 5467, XP000603873
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